Cloning and sequence determination of a phi AAU2 gene whose product aborts the phage lytic cycle.

Abstract:

:This communication describes the cloning of a 1.8-kb fragment from the genome of the corynephage phi AAU2, which aborts the phage lytic cycle when cloned on a high-copy shuttle vector. The associated phenotype, called Apld (aborting phage lytic development), was revealed by noting the reduced plaque size and lower efficiencies of plaquing of phi AAU2 cp, a virulent derivative of phi AAU2, on "Arthrobacter aureus"-C70 Apld+ cells. Adsorption and phage DNA transfection experiments showed evidence that Apld acted once the phage DNA had entered into the cell; apld was confined to a single open reading frame (ORF), encoding a putative 63-aa polypeptide which did not show any homology to proteins contained in the databanks; apld is followed by an ORF the product of which shows homology with a protein expressed by the early region of the Streptomyces phage phi C31.

journal_name

Res Microbiol

journal_title

Research in microbiology

authors

Le Marrec C,Moreau S,Leret V,Schäffer A,Blanco C,Trautwetter A

doi

10.1016/s0923-2508(98)80078-8

subject

Has Abstract

pub_date

1998-03-01 00:00:00

pages

177-88

issue

3

eissn

0923-2508

issn

1769-7123

pii

S0923-2508(98)80078-8

journal_volume

149

pub_type

杂志文章
  • Identification of a methicillin-resistant Staphylococcus aureus inhibitory compound isolated from Serratia marcescens.

    abstract::In this study, we identified an antimicrobial compound produced by the Gram-negative bacterium Serratia marcescens. Colonies of S. marcescens inhibited the growth of nine different methicillin-resistant Staphylococcus aureus (MRSA) isolates and several other tested Gram-positive bacterial species, but not Gram-negativ...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2013.06.002

    authors: Kadouri DE,Shanks RM

    更新日期:2013-10-01 00:00:00

  • Fluorescence enlightens RND pump activity and the intrabacterial concentration of antibiotics.

    abstract::To understand antibiotic resistance in Gram-negative bacteria, a key point is to investigate antibiotic accumulation, which is defined by influx and efflux. Several methods exist to evaluate membrane permeability and efflux pump activity, but they present disadvantages and limitations. An optimized spectrofluorimetric...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/j.resmic.2017.11.005

    authors: Masi M,Dumont E,Vergalli J,Pajovic J,Réfrégiers M,Pagès JM

    更新日期:2018-01-01 00:00:00

  • Mesophilic Aeromonas strains from different serogroups: the influence of growth temperature and osmolarity on lipopolysaccharide and virulence.

    abstract::Growth of mesophilic Aeromonas sp. strains from serogroups O:13, O:33 and O:44 at different temperatures and osmolarity resulted in changes in the lipopolysaccharide (LPS) and virulence of the strains tested, as we had previously reported for strains from serogroup O:34. The effect of osmolarity could be observed when...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/s0923-2508(98)80323-9

    authors: Merino S,Aguilar A,Rubires X,Tomás JM

    更新日期:1998-06-01 00:00:00

  • Evolution of mollicutes: down a bumpy road with twists and turns.

    abstract::Mollicute evolution has been marked by significant changes in genome structure and use of their genetic information. These include a reduction in their genome G+C content and the use by most mollicutes of the UGA universal stop codon as tryptophan. More striking is the size reduction in their genome which, for some sp...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2007.09.007

    authors: Sirand-Pugnet P,Citti C,Barré A,Blanchard A

    更新日期:2007-12-01 00:00:00

  • Second-order selection in bacterial evolution: selection acting on mutation and recombination rates in the course of adaptation.

    abstract::The increase in genetic variability of a population can be selected during adaptation, as demonstrated by the selection of mutator alleles. The dynamics of this phenomenon, named second-order selection, can result in an improved adaptability of bacteria through regulation of all facets of mutation and recombination pr...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/s0923-2508(00)01163-3

    authors: Tenaillon O,Taddei F,Radmian M,Matic I

    更新日期:2001-01-01 00:00:00

  • A century of typhus, lice and Rickettsia.

    abstract::At the beginning of the 20th century, it was discovered at the Pasteur Institute in Tunis that epidemic typhus is transmitted by the human body louse. The complete genome sequence of its causative agent, Rickettsia prowazekii, was determined at Uppsala University in Sweden at the end of the century. In this mini-revie...

    journal_title:Research in microbiology

    pub_type: 传,历史文章,杂志文章,评审

    doi:10.1016/s0923-2508(00)00116-9

    authors: Andersson JO,Andersson SG

    更新日期:2000-03-01 00:00:00

  • Diversity of ribosomal DNA fingerprints of Leptospira serovars provides a database for subtyping and species assignation.

    abstract::Ribosomal DNA fingerprints from 103 pathogenic Leptospira strains were examined using EcoRI restriction and fragment length polymorphisms of rRNA genes. Sixty-nine new leptospiral ribotypes were described, in addition to 49 previously observed. Except for 5 strains, a good correlation between DNA homology data and rib...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(93)90210-s

    authors: Perolat P,Lecuyer I,Postic D,Baranton G

    更新日期:1993-01-01 00:00:00

  • Phenotypic characterization of human and animal biotypes within the species Porphyromonas gingivalis.

    abstract::Ninety-nine strains of Gram-negative black-pigmented anaerobic rods, grown on Todd-Hewitt blood agar plates, were identified and characterized according to a typing scheme including UV fluorescence, catalase, trypsin-like and haemagglutinating activities, biochemical tests with the ATB 32A kit, and gas-liquid chromato...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(93)90051-3

    authors: Fournier D,Mouton C

    更新日期:1993-07-01 00:00:00

  • Adhesion of bacteria to resected human colonic tissue: quantitative analysis of bacterial adhesion and viability.

    abstract::Adhesion to the intestinal mucosa is considered to be one of the main selection criteria of lactic acid bacteria for probiotic use. Adhesive probiotics are, for example, considered to provide better antagonism against pathogenic bacteria when compared to non-adhesive strains. Here a new model is described for studying...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2004.08.012

    authors: Vesterlund S,Paltta J,Karp M,Ouwehand AC

    更新日期:2005-03-01 00:00:00

  • The bacterial phosphotransferase system as a potential vehicle for the entry of novel antibiotics.

    abstract::During the past twenty-nine years, not a single class of antimicrobial agents has been discovered that has led to new approved human drugs. Despite a dramatic increase in the potency of existing classes, the need for new effective antimicrobial agents continues. The bacterial phosphotransferase system (PTS) offers the...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/0923-2508(92)90089-7

    authors: Parr TR Jr,Saier MH Jr

    更新日期:1992-06-01 00:00:00

  • Differentiation of Candida guilliermondii varieties by lectin-like substances from marine algae.

    abstract::Lectin-like substances of 54 marine algae, corresponding to 30 red algae and 24 brown algae, were studied to estimate the agglutination activity against Candida guilliermondii. Extracts of Dictyopteris membranacea, Bifurcaria bifurcata, Halidrys siliquosa, Ascophyllum nodosum, Fucus serratus and F. spiralis algae show...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(89)90013-2

    authors: Fabregas J,Muñoz A,Llovo J,Villa TG

    更新日期:1989-07-01 00:00:00

  • Cra and the control of carbon flux via metabolic pathways.

    abstract::The catabolite repressor-activator (Cra) protein controls the direction of carbon flux through metabolic pathways in enteric bacteria. Cra binds to the control regions of target genes and exerts a negative effect on the expression of genes encoding glycolytic and Entner-Doudoroff enzymes, while exerting a positive eff...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/0923-2508(96)84003-4

    authors: Ramseier TM

    更新日期:1996-07-01 00:00:00

  • Bacterial growth control studied by flow cytometry.

    abstract::By employing flow cytometry, the DNA content and cell size of individual bacterial cells may be determined rapidly and with high precision. Also, the number of DNA replication origins in Escherichia coli cells can be measured after treating the cells with rifampicin together with the cell division inhibitor cephalexin...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(91)90020-b

    authors: Boye E,Løbner-Olesen A

    更新日期:1991-02-01 00:00:00

  • Biocontrol of Bacillus subtilis against Fusarium verticillioides in vitro and at the maize root level.

    abstract::Bacillus species as a group offer several advantages over other bacteria for protection against root pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. The objectives of this work were to determine the ability of strains of Bacillus to inhibit Fusari...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2005.03.001

    authors: Cavaglieri L,Orlando J,Rodríguez MI,Chulze S,Etcheverry M

    更新日期:2005-06-01 00:00:00

  • Nucleotide sequence of nifH regions from Methanobacterium ivanovii and Methanosarcina barkeri 227 and characterization of glnB-like genes.

    abstract::This brief note reports the nucleotide sequences of the second nifH region of Methanobacterium ivanovii and of the two nifH regions of Methanosarcina barkeri 227. In the three cases, nifH is followed by two ORF (open reading frames) similar to ORF105 and ORF128 respectively, which were previously found downstream of M...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(91)90091-n

    authors: Sibold L,Henriquet M,Possot O,Aubert JP

    更新日期:1991-01-01 00:00:00

  • Cross-correlating analyses of mineral-associated microorganisms in an unsaturated packed bed flow-through column test; cell number, activity and EPS.

    abstract::In heap bioleaching and waste-rock dumps, complex microbial communities exist in the flowing and interstitial liquid phases and mineral surface-associated biofilms, often embedded in extracellular polymeric substances (EPS). Microbial activity in the interstitial phase and mineral ore surface facilitates mineral degra...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2020.09.002

    authors: Makaula DX,Huddy RJ,Fagan-Endres MA,Harrison STL

    更新日期:2020-01-01 00:00:00

  • Isolation, purification and partial characterization of antibacterial activities produced by a newly isolated Streptomyces sp. US24 strain.

    abstract::A new actinomycete strain designated US24 producing antibacterial activities against Gram-positive and Gram-negative bacteria was isolated from Tunisian soil. Culture characteristic studies strongly suggested that the US24 strain belonged to the genus Streptomyces. Analysis of the nucleotide sequence of the 16S rRNA g...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/S0923-2508(03)00077-9

    authors: Mellouli L,Ben Ameur-Mehdi R,Sioud S,Salem M,Bejar S

    更新日期:2003-06-01 00:00:00

  • Genetic organisation of the lipopolysaccharide O-antigen biosynthesis region of brucella melitensis 16M (wbk).

    abstract::Brucella spp. are Gram-negative, facultative intracellular bacteria that cause a zoonotic world-wide disease. As in other Gram-negative bacteria, its S-LPS (smooth lipopolysaccharide) is a major determinant of virulence. The Brucella melitensis 16M LPS O-antigen is a homopolymer of 4-formamido-4,6, dideoxymannose. In ...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/s0923-2508(00)90130-x

    authors: Godfroid F,Cloeckaert A,Taminiau B,Danese I,Tibor A,de Bolle X,Mertens P,Letesson JJ

    更新日期:2000-10-01 00:00:00

  • Bacterial and H2O2 stress-induced apoptosis-like events in Cryptococcus neoformans.

    abstract::Cell death induced by adherence of Staphylococcus aureus was observed in Cryptococcus neoformans. To investigate this death mechanism, we examined three factors: actin dynamics using rhodamine-phalloidin staining, accumulation of reactive oxygen species (ROS) via conversion of 2',7'-dichlorofluorescein diacetate to 2'...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2008.07.006

    authors: Ikeda R,Sawamura K

    更新日期:2008-11-01 00:00:00

  • Real-time PCR for characterizing the stress response of Oenococcus oeni in a wine-like medium.

    abstract::The tolerance of the lactic acid bacterium Oenococcus oeni to hostile wine conditions is essential for the success of malolactic fermentation (MLF). In this study, reverse transcription quantitative PCR (RT-qPCR) was used to quantify the transcript level of 13 genes that could play a role in adaptation of O. oeni in w...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2005.07.006

    authors: Beltramo C,Desroche N,Tourdot-Maréchal R,Grandvalet C,Guzzo J

    更新日期:2006-04-01 00:00:00

  • Life and death of dried prokaryotes.

    abstract::The removal of water through air drying damages membranes, proteins and nucleic acids and is lethal to the majority of organisms. Nevertheless, some vegetative cells of bacteria and cyanobacteria survive extreme desiccation. Understanding the mechanisms of their desiccation tolerance is an important issue in cell biol...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/s0923-2508(01)01279-7

    authors: Billi D,Potts M

    更新日期:2002-01-01 00:00:00

  • How François Jacob bridged the gap between the "two cultures".

    abstract::While the scientific contributions of François Jacob were outstanding, I also consider that his conception of science, and of its place among other forms of knowledge, is also highly original, and important for the future of science in our societies. His contributions to the history and philosophy of science were neit...

    journal_title:Research in microbiology

    pub_type: 传,历史文章,杂志文章

    doi:10.1016/j.resmic.2014.05.001

    authors: Morange M

    更新日期:2014-06-01 00:00:00

  • Microbial community profiling of the Chinoike Jigoku ("Blood Pond Hell") hot spring in Beppu, Japan: isolation and characterization of Fe(III)-reducing Sulfolobus sp. strain GA1.

    abstract::Chinoike Jigoku ("Blood Pond Hell") is located in the hot spring town of Beppu on the southern island of Kyushu in Japan, and is the site of a red-colored acidic geothermal pond. This study aimed to investigate the microbial population composition in this extremely acidic environment and to isolate/characterize acidop...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2016.04.011

    authors: Masaki Y,Tsutsumi K,Hirano S,Okibe N

    更新日期:2016-09-01 00:00:00

  • A triclosan-ciprofloxacin cross-resistant mutant strain of Staphylococcus aureus displays an alteration in the expression of several cell membrane structural and functional genes.

    abstract::Triclosan is an antimicrobial agent found in many consumer products. Triclosan inhibits the bacterial fatty acid biosynthetic enzyme, enoyl-ACP reductase (FabI). Decreased susceptibility to triclosan correlates with ciprofloxacin resistance in several bacteria. In these bacteria, resistance to both drugs maps to genes...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2007.09.003

    authors: Tkachenko O,Shepard J,Aris VM,Joy A,Bello A,Londono I,Marku J,Soteropoulos P,Peteroy-Kelly MA

    更新日期:2007-10-01 00:00:00

  • Structure and role of coupling proteins in conjugal DNA transfer.

    abstract::Type IV secretory systems are transmembrane bacterial multiprotein complexes. They are pivotal for conjugation, bacterial-induced plant tumour formation, toxin secretion and mammalian pathogen intracellular activity. These systems are involved in the spread of antibiotic resistance genes among bacteria by enabling con...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/s0923-2508(02)01313-x

    authors: Gomis-Rüth FX,de la Cruz F,Coll M

    更新日期:2002-05-01 00:00:00

  • Transcriptome analysis of salt-stressed Deinococcus radiodurans and characterization of salt-sensitive mutants.

    abstract::Deinococcus radiodurans is a bacterium best known for its extreme resistance to high levels of ionizing radiation. Gene expression profiles of D. radiodurans exposed to 0.3 M NaCl revealed that at least 389 genes were induced and 415 were repressed by twofold or more. A general down-regulation of the central metabolic...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2013.07.005

    authors: Im S,Joe M,Kim D,Park DH,Lim S

    更新日期:2013-11-01 00:00:00

  • Shaping bacterial genomes with integrative and conjugative elements.

    abstract::Integrative and conjugative elements (ICEs) are self-transmissible mobile genetic elements that are increasingly recognized to contribute to lateral gene flow in prokaryotes. ICEs, like most temperate bacteriophages integrate into the genome and like conjugative plasmids disseminate by conjugative transfer to new host...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/j.resmic.2004.01.012

    authors: Burrus V,Waldor MK

    更新日期:2004-06-01 00:00:00

  • Antiproliferative activity of Actinobacillus (Haemophilus) actinomycetemcomitans and Fusobacterium nucleatum in peripheral blood mononuclear cells.

    abstract::Several studies indicate Actinobacillus (Haemophilus) actinomycetemcomitans and Fusobacterium nucleatum as etiologic agents of periodontal disease. Immunosuppressive factors produced by microorganisms probably contribute to the initiation and evolution of this disease. This study evaluated the antiproliferative activi...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2004.05.008

    authors: do Vale CH,de Oliveira Fraga LA,Costa AS,Tavares CA,Martins-Filho OA,de Macedo Farias L,Roque de Carvalho MA

    更新日期:2004-11-01 00:00:00

  • Microbiological and histological study of the gastrointestinal tract of germ-free mice infected with Helicobacter trogontum.

    abstract::Helicobacter spp. have been the focus of considerable research because of the role of this genus in gastrointestinal diseases. We infected NIH germ-free mice with Helicobacter trogontum, a recently described intestinal bacterium of rats, in order to study the distribution of this bacterium in the gastrointestinal trac...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/s0923-2508(99)80037-0

    authors: Moura SB,Mendes EN,Queiroz DM,Nicoli JR,Cabral MM,Magalhaes PP,Rocha GA,Vieira EC

    更新日期:1999-04-01 00:00:00

  • Protective and therapeutic application of the depolymerase derived from a novel KN1 genotype of Klebsiella pneumoniae bacteriophage in mice.

    abstract::Klebsiella pneumoniae is one of the major Gram-negative bacterial pathogens causing hospital-acquired multidrug-resistant infections, and the antimicrobial treatment options are scarce. The lack of available antimicrobials has prompted the development of alternative strategies for the treatment of these infections. In...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2019.01.003

    authors: Wang C,Li P,Niu W,Yuan X,Liu H,Huang Y,An X,Fan H,Zhangxiang L,Mi L,Zheng J,Liu Y,Tong Y,Mi Z,Bai C

    更新日期:2019-01-01 00:00:00