Abstract：
:Chinoike Jigoku ("Blood Pond Hell") is located in the hot spring town of Beppu on the southern island of Kyushu in Japan, and is the site of a red-colored acidic geothermal pond. This study aimed to investigate the microbial population composition in this extremely acidic environment and to isolate/characterize acidophilic microorganism with metal-reducing ability. Initially, PCR (using bacteria- and archaea-specific primers) of environmental DNA samples detected the presence of bacteria, but not archaea. This was followed by random sequencing analysis, confirming the presence of wide bacterial diversity at the site (123 clones derived from 18 bacterial and 1 archaeal genera), including those closely related to known autotrophic and heterotrophic acidophiles (Acidithiobacillus sp., Sulfobacillus sp., Alicyclobacillus sp.). Nevertheless, successive culture enrichment with Fe(III) under micro-aerobic conditions led to isolation of an unknown archaeal organism, Sulfolobus sp. GA1 (with 99.7% 16S rRNA gene sequence identity with Sulfolobus shibatae). Unlike many other known Sulfolobus spp., strain GA1 was shown to lack sulfur oxidation ability. Strain GA1 possessed only minor Fe(II) oxidation ability, but readily reduced Fe(III) during heterotrophic growth under micro-aerobic conditions. Strain GA1 was capable of reducing highly toxic Cr(VI) to less toxic/soluble Cr(III), demonstrating its potential utility in bioremediation of toxic metal species.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Masaki Y,Tsutsumi K,Hirano S,Okibe Ndoi
10.1016/j.resmic.2016.04.011subject
Has Abstractpub_date
2016-09-01 00:00:00pages
595-603issue
7eissn
0923-2508issn
1769-7123pii
S0923-2508(16)30013-4journal_volume
167pub_type
杂志文章abstract：:Heterotrophic microaerophilic bacteria, a diverse and ubiquitous group, are specialized for growth in O2 limited environments. Until recently, microaerophilic bacteria have been grouped with obligate aerobic organisms whose metabolic rates slow in response to physiological O2 limitation. In contrast, microaerophilic b...
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pub_type： 杂志文章,评审
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doi：10.1016/j.resmic.2008.05.004
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pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80037-0
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pub_type： 杂志文章
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更新日期：1998-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.resmic.2011.06.004
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pub_type： 杂志文章
doi：10.1016/0923-2508(90)90082-2
更新日期：1990-11-01 00:00:00
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journal_title：Research in microbiology
pub_type： 临床试验,杂志文章,评审
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更新日期：1990-09-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.07.008
更新日期：2007-10-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(94)90078-7
更新日期：1994-10-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(98)80003-x
更新日期：1998-07-01 00:00:00
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doi：10.1016/j.resmic.2010.09.013
更新日期：2010-12-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
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更新日期：1997-01-01 00:00:00
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journal_title：Research in microbiology
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更新日期：2014-11-01 00:00:00
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更新日期：2010-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0923-2508(89)90086-7
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abstract：:Vegetative incompatibility limits heterokaryon formation in fungi. It results from genetic differences at specific loci (het loci). Characterization of het genes and, more recently, of incompatibility reaction suppressors has, provided insight into the mechanisms involved. A link between development, vegetative incomp...
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pub_type： 杂志文章,评审
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更新日期：2000-05-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
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pub_type： 杂志文章
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更新日期：1995-03-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
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更新日期：2003-11-01 00:00:00
abstract：:Although great strides have been made in the development of recombinant attenuated Vibrio cholerae vaccine strains, the task has not been as simple as once imagined. The initial vaccine candidates proved to be unexpectedly reactogenic but further derivatives, such as CVD103-HgR, are well-tolerated, immunogenic and pro...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(90)90128-d
更新日期：1990-09-01 00:00:00
