Isolation, purification and partial analysis of the lipopolysaccharide antigenic determinant recognized by a monoclonal antibody to Legionella pneumophila serogroup 1.

abstract：:Replicon typing is the identification of plasmids by hybridization with specific DNA probes which contain the genes involved in plasmid maintenance. This new method has been used to classify plasmids into replicon (rep) groups which can often be correlated with incompatibility (Inc) groups. We studied 71 multiresistan...

journal_title：Research in microbiology

authors： Llanes C,Couturier M,Asfeld L,Grimont F,Michel-Briand Y

更新日期：1994-01-01 00:00:00

abstract：:Bacteria of the Bacillus cereus group are environmental Gram-positive spore-forming bacteria ubiquitously distributed. Despite the high degree of genetic similarity among the different strains, they show strong phenotypic variability, from mammal or entomopathogen strains to soil-dwelling saprophytes, and from psychro...

journal_title：Research in microbiology

authors： Patiño-Navarrete R,Sanchis V

更新日期：2017-05-01 00:00:00

abstract：:Viruses play important roles in aquatic ecosystems, but deep-sea bacteriophages remain largely unexplored. A temperate bacteriophage (termed vB_PstS-1) was identified from the psychrotolerant gammaproteobacterium Pseudomonas stutzeri 1-1-1b, which was isolated from hadopelagic water (depth of 7000 m) of the Japan Tren...

journal_title：Research in microbiology

authors： Yoshida M,Yoshida-Takashima Y,Nunoura T,Takai K

更新日期：2015-11-01 00:00:00

abstract：:The expression of the feruloyl esterase gene faeA, the alpha-glucuronidase gene aguA, the endoxylanase gene xlnB, and the beta-xylosidase gene xlnD from Aspergillus niger on xylose was studied in a wild-type strain and in a CreA mutant. A decrease in expression of all four genes was observed with increasing xylose con...

journal_title：Research in microbiology

authors： de Vries RP,Visser J,de Graaff LH

更新日期：1999-05-01 00:00:00

abstract：:Ninety-nine strains of Gram-negative black-pigmented anaerobic rods, grown on Todd-Hewitt blood agar plates, were identified and characterized according to a typing scheme including UV fluorescence, catalase, trypsin-like and haemagglutinating activities, biochemical tests with the ATB 32A kit, and gas-liquid chromato...

journal_title：Research in microbiology

authors： Fournier D,Mouton C

更新日期：1993-07-01 00:00:00

abstract：:A strain of Bacteroides fragilis, isolated from the marmoset Callithrix penicillata, produced protein(s) with bacteriocin activity (fragicilin). Two active fractions (36 and 150 kDa) were isolated by chromatography. The bacteriocin exhibited iso- and heteroantagonism. It remained stable between pH 3 and 10 and at 60 d...

journal_title：Research in microbiology

authors： Farias LM,Totola AH,Miranda CM,Carvalho MA,Damasceno CA,Tavares CA,Cisalpino EO,Vieira EC

更新日期：1994-01-01 00:00:00

abstract：:A common effluent treatment plant (CETP) is a biological wastewater treatment facility that receives wastewater from different industries. The activated biomass in the CETP survives on a wide range of chemicals with no fixed wastewater characteristics. We carried out a diversity analysis of this activated biomass usin...

journal_title：Research in microbiology

authors： Kapley A,De Baere T,Purohit HJ

更新日期：2007-07-01 00:00:00

abstract：:Campylobacter spp. is the most common cause of bacterial diarrhoea in humans worldwide. Therefore, rapid and reliable methods for detection and quantification of this pathogen are required. In this study, we have developed a reverse transcription quantitative real-time PCR (RT-qPCR) for detection and quantification of...

journal_title：Research in microbiology

authors： Bui XT,Wolff A,Madsen M,Bang DD

更新日期：2012-01-01 00:00:00

abstract：:Bordetella isolates in the Saint Petersburg region have been monitored since 1998. Over the past ten years, concomitant with the increase in pertussis whole-cell vaccine coverage, the incidence of whooping cough has decreased. However, this decrease exists only for Bordetella pertussis infections, as the incidence of ...

journal_title：Research in microbiology

authors： Kurova N,Njamkepo E,Brun D,Tseneva G,Guiso N

更新日期：2010-12-01 00:00:00

abstract：:Monopolization of the vaginal econiche by a limited number of Lactobacillus species, resulting in low pH of 3.5-4.5, has been shown to protect women against vaginal dysbiosis, sexually transmitted infections and adverse pregnancy outcomes. Still, controversy exists as to which characteristics of lactobacilli are most ...

journal_title：Research in microbiology

authors： Vaneechoutte M

更新日期：2017-01-01 00:00:00

abstract：:Quorum sensing is a cell-density-dependent regulatory mechanism which, in Gram-negative bacteria, usually involves the production and detection of N-acyl homoserine lactones (HSLs). In the last four years HSL-dependent quorum sensing has been identified in members of the Burkholderia cepacia complex, and this mini-rev...

journal_title：Research in microbiology

authors： Venturi V,Friscina A,Bertani I,Devescovi G,Aguilar C

更新日期：2004-05-01 00:00:00

abstract：:The chitinolytic activity of Verticillium cfr. lecanii A3, a strain isolated from continental Antarctica, was compared to those of two selected strains of Trichoderma harzianum. After 72 h of incubation at 25 degrees C in media containing chitin as the sole carbon source, all strains showed the same enzyme activity (c...

journal_title：Research in microbiology

authors： Fenice M,Selbmann L,Di Giambattista R,Federici F

更新日期：1998-04-01 00:00:00

abstract：:Ribosomal DNA fingerprints from 103 pathogenic Leptospira strains were examined using EcoRI restriction and fragment length polymorphisms of rRNA genes. Sixty-nine new leptospiral ribotypes were described, in addition to 49 previously observed. Except for 5 strains, a good correlation between DNA homology data and rib...

journal_title：Research in microbiology

authors： Perolat P,Lecuyer I,Postic D,Baranton G

更新日期：1993-01-01 00:00:00

abstract：:The adsorption complex of filamentous phage fd consists of two minor coat proteins, g3p and g6p, and is considered to be not only a structural entity, but also a functional unit to terminate phage assembly. Cells were infected with phage M13am8H1, which cannot assemble because it lacks the major coat protein g8p, alth...

journal_title：Research in microbiology

authors： Gailus V,Ramsperger U,Johner C,Kramer H,Rasched I

更新日期：1994-11-01 00:00:00

abstract：:The results of a multiple locus variable number of tandem repeat (VNTR) analysis (MLVA)-based study designed to understand the genetic diversity of soil and manure-borne Pseudomonas aeruginosa isolates, and the relationship between these isolates and a set of clinical and environmental isolates, are hereby reported. F...

journal_title：Research in microbiology

authors： Youenou B,Brothier E,Nazaret S

更新日期：2014-01-01 00:00:00

abstract：:We present evidence that there are at least three Aspergillus nidulans enzymes which catalyze in vitro the reaction of O-acetylserine (OAS) with sulfide forming cysteine. This activity is shared by cysteine synthase (CS) encoded by the cysB gene, homocysteine synthase encoded by cysD and by at least one more enzyme. M...

journal_title：Research in microbiology

authors： Brzywczy J,Natorff R,Sieńko M,Paszewski A

更新日期：2007-06-01 00:00:00

abstract：:By employing flow cytometry, the DNA content and cell size of individual bacterial cells may be determined rapidly and with high precision. Also, the number of DNA replication origins in Escherichia coli cells can be measured after treating the cells with rifampicin together with the cell division inhibitor cephalexin...

journal_title：Research in microbiology

authors： Boye E,Løbner-Olesen A

更新日期：1991-02-01 00:00:00

abstract：:The aim of the present work was to develop polymerase chain reactions (PCRs) based on the conserved nucleotide sequence of the 16S rRNA gene for detection of bacteria of the Helicobacter genus in human antral biopsy samples. The assay for Helicobacter spp was developed by amplifying a 399-bp 16S rRNA gene sequence spe...

journal_title：Research in microbiology

authors： Germani Y,Dauga C,Duval P,Huerre M,Levy M,Pialoux G,Sansonetti P,Grimont PA

更新日期：1997-05-01 00:00:00

abstract：:Mesophilic iron and sulfur-oxidizing acidophiles are readily found in acid mine drainage sites and bioleaching operations, but relatively little is known about their activities at suboptimal temperatures and in cold environments. The purpose of this work was to characterize the oxidation of elemental sulfur (S(0)), te...

journal_title：Research in microbiology

authors： Kupka D,Liljeqvist M,Nurmi P,Puhakka JA,Tuovinen OH,Dopson M

更新日期：2009-12-01 00:00:00

abstract：:Spirochetes have a unique motility system that is characterized by flagellar filaments contained within the outer membrane sheath. Direct evidence using video microscopy has recently been obtained which indicates that these periplasmic flagella (PF) rotate in several spirochetal species. This rotation generates thrust...

journal_title：Research in microbiology

authors： Charon NW,Greenberg EP,Koopman MB,Limberger RJ

更新日期：1992-07-01 00:00:00

abstract：:The tolerance of the lactic acid bacterium Oenococcus oeni to hostile wine conditions is essential for the success of malolactic fermentation (MLF). In this study, reverse transcription quantitative PCR (RT-qPCR) was used to quantify the transcript level of 13 genes that could play a role in adaptation of O. oeni in w...

journal_title：Research in microbiology

authors： Beltramo C,Desroche N,Tourdot-Maréchal R,Grandvalet C,Guzzo J

更新日期：2006-04-01 00:00:00

abstract：:The chromosome of Escherichia coli is negatively supercoiled. This favours processes that unwind the two DNA strands, such as DNA replication. In this paper, we have investigated the effect of changed levels of overall chromosomal supercoiling on the initiation of DNA replication. Specifically, we have used flow cytom...

journal_title：Research in microbiology

authors： von Freiesleben U,Rasmussen KV

更新日期：1992-09-01 00:00:00

abstract：:Macroscopic and microscopic measurements of the growth of Trichoderma viride and Rhizopus oligosporus were compared at different values of water activity of the solid medium. The relationship between radial extension rate and hyphal growth rate was found to be dependent on water activity. The observed difference betwe...

journal_title：Research in microbiology

authors： Molin P,Gervais P,Lemière JP,Davet T

更新日期：1992-10-01 00:00:00

abstract：:The marine psychrophilic bacterium Moritella viscosa is the causative agent of winter ulcer in farmed Atlantic salmon and cod. In this study, the growth requirements of the pathogen were established. The effects of changes in salinity and temperature on growth, surface features and proteomic regulation were also inves...

journal_title：Research in microbiology

authors： Tunsjø HS,Paulsen SM,Mikkelsen H,L'abée-Lund TM,Skjerve E,Sørum H

更新日期：2007-04-01 00:00:00

abstract：:The sensitivity and specificity of 7 PCR assays described for the identification of Campylobacter jejuni and Campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of C. jejuni, C. coli, Campylobacter lari and related Campylobacter, Helicobacter and A...

journal_title：Research in microbiology

authors： Debruyne L,Samyn E,De Brandt E,Vandenberg O,Heyndrickx M,Vandamme P

更新日期：2008-03-01 00:00:00

abstract：:The neurotoxin gene from a strain of Clostridium botulinum type A causing infant botulism was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated using primers designed to conserved regions of published botulinal toxin (BoNT) sequences. Translation of the nucleotide sequence derived f...

journal_title：Research in microbiology

authors： Willems A,East AK,Lawson PA,Collins MD

更新日期：1993-09-01 00:00:00

abstract：:The naturally transformable bacterium Haemophilus influenzae Rd contains 1471 copies of the DNA uptake signal sequence (USS) 5'-AAGTGCGGT in its genome. Neisseria meningitidis contains 1891 copies of the USS sequence 5'-GCCGTCTGAA. The USSs are often found in the base paired stem of transcription terminators. ...

journal_title：Research in microbiology

authors： Smith HO,Gwinn ML,Salzberg SL

更新日期：1999-11-01 00:00:00

abstract：:In Neisseria gonorrhoeae, all penicillinase-specifying plasmids isolated so far share homology with each other and carry a 60% deleted sequence of TnA. Plasmid pHD131, an element isolated from Haemophilus ducreyi and carrying an intact copy of the ampicillin resistance transposable element, was introduced from Escheri...

journal_title：Research in microbiology

authors： Piffaretti JC,Soldati L

更新日期：1990-06-01 00:00:00

abstract：:Six thermophilic extremophiles, Anoxybacillus amylolyticus, Geobacillus thermoleovorans, Geobacillus thermoleovorans subspecies stromboliensis, Geobacillus toebii subspecies decanicus, Bacillus thermantarcticus and Thermus oshimai, isolated from different environmental sites, were studied for their heavy metal resista...

journal_title：Research in microbiology

authors： Poli A,Salerno A,Laezza G,di Donato P,Dumontet S,Nicolaus B

更新日期：2009-03-01 00:00:00

abstract：:Four monoclonal antibodies against chlamydial lipopolysaccharide (LPS) were used to study their localization and distribution in the Chlamydia psittaci AB7 abortion-causing strain by immunoelectron microscopy. A non-embedding technique on whole chlamydiae, together with a post-embedding technique on McCoy cells infect...

journal_title：Research in microbiology

authors： Salinas J,Sanchez J,Buendia AJ,Souriau A,Rodolakis A,Bernabé A,Cuello F

更新日期：1994-10-01 00:00:00