Abstract：
:The adsorption complex of filamentous phage fd consists of two minor coat proteins, g3p and g6p, and is considered to be not only a structural entity, but also a functional unit to terminate phage assembly. Cells were infected with phage M13am8H1, which cannot assemble because it lacks the major coat protein g8p, although producing all of the other minor coat proteins. The membranes of infected cells were solubilized and analysed by non-denaturing PAGE and gel filtration. The data suggest the presence of the adsorption complex in these membranes. Furthermore, the non-polar gene 3 amber-mutant phage R171 was shown to lack g6p in the phage coat as well. The termination of assembly of this phage is disturbed, resulting in synthesis of polyphages. Electron micrographs and transient electrical birefringence show that these polyphages are eight times longer as compared to unit length phage. From these results, we conclude that the formation of the g3p-g6p complex is essential for correct termination of filamentous phage assembly.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Gailus V,Ramsperger U,Johner C,Kramer H,Rasched Idoi
10.1016/0923-2508(94)90042-6subject
Has Abstractpub_date
1994-11-01 00:00:00pages
699-709issue
9eissn
0923-2508issn
1769-7123pii
0923-2508(94)90042-6journal_volume
145pub_type
杂志文章abstract：:Haemophilus parainfluenzae and Haemophilus influenzae, upper respiratory tract microbiota representatives, are able to colonize natural and artificial surfaces as biofilm. The aim of the present study was to assay the effect of ten 1,2,4-triazole-ciprofloxacin hybrids on planktonic or biofilm-forming haemophili cells ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.05.009
更新日期：2016-10-01 00:00:00
abstract：:Bacillus cereus is widely distributed in the environment and is responsible for foodborne illnesses, causing diarrhea and vomiting. In this study, the prevalence of B. cereus bacteriophages in Korean fermented foods was studied using 6 reference strains, and JBP901 was further characterized. In a total of 47 samples, ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.07.001
更新日期：2011-10-01 00:00:00
abstract：:Some of the present problems in ribotyping are associated with a lack of uniform reactivity of probes when bacterial DNAs are of phylogenetically diverse origins. To overcome these problems, a set of five oligonucleotides (referred to as OligoMix5) was selected to react with conserved sequences located near both extre...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(99)80064-3
更新日期：1997-11-01 00:00:00
abstract：:Most recently published complete bacterial genomes have revealed unexpectedly high numbers of long strict repeats. In this article we discuss the various functional and evolutionary roles of these repeats, focusing in particular on their role in terms of genome stability, gene transfer, and antigenic variation. ...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(99)00120-5
更新日期：1999-11-01 00:00:00
abstract：:Energy-coupling factor (ECF) transporters mediate the uptake of micronutrients in prokaryotes. They consist of two ATP-binding-cassette family ATPases, a transmembrane coupling protein (T component) and a substrate-binding membrane protein (S component). ECF transporters for Co2+ and Ni2+ ions have one or two addition...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2019.06.007
更新日期：2019-01-01 00:00:00
abstract：:The information collected in national and international libraries on nucleotide and protein sequences cannot be directly treated for proper handling by existing software. Therefore we evaluated the feasibility of constructing a data base for Escherichia coli using the data present in the banks. The knowhow thus acquir...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(91)90073-j
更新日期：1991-09-01 00:00:00
abstract：:The insertion of proteins into membranes generally requires the assistance of membrane proteins. A protein, designated Omp85 in Neisseria meningitidis, was shown to be required for the assembly of bacterial outer-membrane proteins. The protein is essential for the viability of the bacteria and is ubiquitous among Gram...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2003.11.007
更新日期：2004-04-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90024-7
更新日期：1989-05-01 00:00:00
abstract：:5-Mycoloyl-beta-arabinofuranosyl-(1-->2)-5-mycoloyl-alpha-ar abinofuranosyl-(1-->1')-glycerol, an antigenic glycolipid from the Mycobacterium avium-intracellulare complex (MAC) was examined for its applicability to the serodiagnosis of MAC infection by ELISA. Serum IgM antibody titres against this glycolipid in health...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(93)90048-7
更新日期：1993-03-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)80565-1
更新日期：1995-09-01 00:00:00
abstract：:Production of aerobactin has been reported to be a virulence factor in members of the family Enterobacteriaceae. To investigate the protection afforded by humoral immunity directed towards aerobactin in infectious diseases caused by aerobactin-producing strains, we tested the efficacy of mAbAERO1, a murine monoclonal ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)80894-1
更新日期：1995-02-01 00:00:00
abstract：:Several cDNA fragments corresponding to transcripts differentially expressed under conditions that favor mycelial growth of Candida albicans were identified by the "differential display" technique. One of these was cloned and used as a probe to rescue the full gene from a genomic library of the fungus. The sequence id...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(02)01373-6
更新日期：2002-11-01 00:00:00
abstract：:Pathogenic Vibrio cholerae strains were compared by fingerprinting with arbitrarily primed polymerase chain reaction (AP-PCR). They were O1 classical and El Tor strains and recent non-O1 Bengal strains. Ten oligonucleotides from a total of fifty-two tested gave distinctive patterns, and these strains were separated in...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)81064-3
更新日期：1995-10-01 00:00:00
abstract：:The trehalose operon of Pseudomonas fluorescens ATCC 17400 consists of treP, treA and treR. The gene treP codes for a putative enzyme II subunit of the phosphotransferase system that catalyzes the phosphorylation of trehalose together with its translocation across the cell membrane and treA encodes a putative phosphot...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)01151-7
更新日期：2000-12-01 00:00:00
abstract：:Helicobacter spp. have been the focus of considerable research because of the role of this genus in gastrointestinal diseases. We infected NIH germ-free mice with Helicobacter trogontum, a recently described intestinal bacterium of rats, in order to study the distribution of this bacterium in the gastrointestinal trac...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80037-0
更新日期：1999-04-01 00:00:00
abstract：:Ammonia-oxidizing archaea (AOA) and bacteria (AOB) are widely distributed in the natural environment and play crucial roles in the nitrification process and the removal of nitrogen (N). Although planktonic microbial community plays an important role in river biogeochemical cycles, few studies have attempted to address...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.08.003
更新日期：2014-10-01 00:00:00
abstract：:From the nose of a patient with aspiration pneumonia, we isolated a new bacterium by amoebal co-culture using Acanthamoeba polyphaga. Based on phenotypic, 16S rRNA and phylogenetic analysis, we show that this isolate represents a new species within the genus Rhodobacter and and we have named it Rhodobacter massiliensi...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2003.08.002
更新日期：2003-11-01 00:00:00
abstract：:The mechanism by which TrwB acts as a DNA transporter in bacterial conjugation is analyzed. Based on a parallelism between TrwB and F(1)-ATPase, TrwB would use the energy derived from ATP hydrolysis to pump DNA through its central channel, in a manner similar to that used by F(1)-ATPase to produce a rotary movement of...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2005.12.002
更新日期：2006-05-01 00:00:00
abstract：:Identification and classification of bacteriophages remains a cumbersome process even with the use of genotypic approaches, due to the lack of genes present in all phages. Restriction fragment length polymorphism analysis (RFLP) of the viral genome is a universal approach, but RFLP fingerprints obtained on agarose gel...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.06.002
更新日期：2007-09-01 00:00:00
abstract：:Although chromosomal replication is an essential feature of the bacterial life cycle, the replication mechanism and involved molecular players have never been properly characterized in the Acetobacter genera. Thanks to whole-genome sequencing, the unknown replication proteins from Acetobacter pasteurianus and Acetobac...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.06.010
更新日期：2016-10-01 00:00:00
abstract：:The development of nascent cell division sites was studied in Escherichia coli strains containing ftsAts and ftsZts mutations in which septal development is arrested after shift to the restrictive temperature. Division sites were studied by measuring positions of plasmolysis bays, a visible marker for periseptal annul...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(91)90047-e
更新日期：1991-02-01 00:00:00
abstract：:In this study, we identified an antimicrobial compound produced by the Gram-negative bacterium Serratia marcescens. Colonies of S. marcescens inhibited the growth of nine different methicillin-resistant Staphylococcus aureus (MRSA) isolates and several other tested Gram-positive bacterial species, but not Gram-negativ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.06.002
更新日期：2013-10-01 00:00:00
abstract：:A method which enables selection of protein mutants impaired in their ability to interact with their normal protein partners is presented here. The method is the two-phage two-hybrid assay adapted for mutant selection. In the two-phage assay, the interaction between two proteins enables the formation of a functional h...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2010.01.001
更新日期：2010-03-01 00:00:00
abstract：:Rhizobium leguminosarum bv. trifolii synthesizes extracellular polysaccharide (EPS) that is postulated to be a biologically active signalling molecule in clover symbiosis. A group of seven exopolysaccharide-deficient (Exo), non-nitrogen-fixing mutants of R. leguminosarum bv. trifolii strain 24.1 isolated by transposon...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(03)00113-X
更新日期：2003-07-01 00:00:00
abstract：:A variety of pathways for carbon and electron flow in the bacterium Escherichia coli and in other enteric bacteria are differentially expressed depending on whether molecular oxygen is present in the cell environment. This review briefly summarizes the metabolic pathways operative during aerobic versus anaerobic cell ...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(94)90092-2
更新日期：1994-06-01 00:00:00
abstract：:The chitinolytic activity of Verticillium cfr. lecanii A3, a strain isolated from continental Antarctica, was compared to those of two selected strains of Trichoderma harzianum. After 72 h of incubation at 25 degrees C in media containing chitin as the sole carbon source, all strains showed the same enzyme activity (c...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(98)80304-5
更新日期：1998-04-01 00:00:00
abstract：:The major 25-kDa outer membrane protein (Omp25) of Brucella abortus was purified and antigenically characterized by use of monoclonal antibodies (mAbs). Purification was achieved from the sodium dodecyl sulphate-insoluble (SDS-I) cell wall (CW) fraction of vaccine strain B. abortus B19 which was shown by use of mAbs t...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)81383-0
更新日期：1996-05-01 00:00:00
abstract：:There is still an urgent requirement for more sensitive, cost-effective methods for detection and susceptibility testing of mycobacteria in clinical samples. We have been investigating a simple bacteriophage-based system which could be used for both purposes. As this depends upon the detection of phages which have suc...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(98)80003-x
更新日期：1998-07-01 00:00:00
abstract：:Species of Alicyclobacillus, Bacillus and Thermus genera were selected in order to study the possible presence of the (ADP-ribosyl)ation system. These bacteria are thermophilic, aerobic, and were isolated from different geothermal sources. Both activity and expression of (ADP-ribosyl)ating proteins were tested in cell...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.01.004
更新日期：2006-07-01 00:00:00
abstract：:Tellurite-containing media are widely used for the screening and isolation of Shiga toxin-producing Escherichia coli (STEC) O157:H7, but tellurite resistance among non-O157 STEC is poorly characterized. Therefore, we investigated 202 STEC strains representing 61 different serotypes from humans, animals or food for the...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.10.007
更新日期：2007-03-01 00:00:00
