Abstract：
:Haemophilus parainfluenzae and Haemophilus influenzae, upper respiratory tract microbiota representatives, are able to colonize natural and artificial surfaces as biofilm. The aim of the present study was to assay the effect of ten 1,2,4-triazole-ciprofloxacin hybrids on planktonic or biofilm-forming haemophili cells in vitro under stationary conditions on the basis of MICs (minimal inhibitory concentrations) and MBICs (minimal biofilm inhibitory concentrations). In addition, anti-adhesive properties of these compounds were examined. The reference strains of H. parainfluenzae and H. influenzae were included. The broth microdilution microtiter plate (MTP) method with twofold dilution of the compounds, or ciprofloxacin (reference agent) in 96-well polystyrene microplates, was used. The optical density (OD) reading was made spectrophotometrically at a wavelength of 570 nm (OD570) both to measure bacterial growth and to detect biofilm-forming cells under the same conditions with 0.1% crystal violet. The following values of parameters were estimated for 1,2,4-triazole-ciprofloxacin hybrids - MIC = 0.03-15.63 mg/L, MBIC = 0.03-15.63 mg/L, MBIC/MIC = 0.125-8, depending on the compound, and for ciprofloxacin - MIC = 0.03-0.06 mg/L, MBIC = 0.03-0.12 mg/L, MBIC/MIC = 1-2. The observed strong anti-adhesive properties (95-100% inhibition) of the tested compounds were reversible during long-term incubation at subinhibitory concentrations. Thus, 1,2,4-triazole-ciprofloxacin hybrids may be considered as starting compounds for designing improved agents not only against planktonic but also against biofilm-forming Haemophilus spp. cells.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Kosikowska U,Andrzejczuk S,Plech T,Malm Adoi
10.1016/j.resmic.2016.05.009subject
Has Abstractpub_date
2016-10-01 00:00:00pages
647-654issue
8eissn
0923-2508issn
1769-7123pii
S0923-2508(16)30049-3journal_volume
167pub_type
杂志文章abstract：:Formation of organized colony morphology is clearly a result of organized, coordinated behavior of cells within a colony, which reflects changes in the cell environment, nutrient availability, inter- and intracolony signaling and others. Under standard conditions, colony morphology is specific to the particular yeast ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.05.012
更新日期：2005-11-01 00:00:00
abstract：:The gene encoding a lipoprotein of 67 kDa, named P67, was cloned from Mycoplasma sp. bovine group 7 strain PG50 and expressed in Escherichia coli K12. Analysis of the amino acid sequence derived from the DNA sequence of the P67 gene revealed a typical prokaryotic signal peptidase II membrane lipoprotein lipid attachme...
journal_title：Research in microbiology
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doi：10.1016/s0923-2508(97)83624-8
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abstract：:A method which enables selection of protein mutants impaired in their ability to interact with their normal protein partners is presented here. The method is the two-phage two-hybrid assay adapted for mutant selection. In the two-phage assay, the interaction between two proteins enables the formation of a functional h...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2010.01.001
更新日期：2010-03-01 00:00:00
abstract：:Pathogenic Vibrio cholerae strains were compared by fingerprinting with arbitrarily primed polymerase chain reaction (AP-PCR). They were O1 classical and El Tor strains and recent non-O1 Bengal strains. Ten oligonucleotides from a total of fifty-two tested gave distinctive patterns, and these strains were separated in...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)81064-3
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.06.004
更新日期：2011-11-01 00:00:00
abstract：:The Escherichia coli mannitol permease (EIIMtl) of the phosphoenolpyruvate-dependent phosphotransferase system is a 68-kDa membrane protein that carries out the concomitant transport and phosphorylation of D-mannitol. Previous studies indicated that there are ca. 6 membrane-spanning helices within the N-terminal half ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90003-7
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abstract：:The biological complexity of trade-offs has been a major obstacle in understanding bacterial diversity and coexistence. Here we reduce the biological complexity by using isogenic Escherichia coli strains differing only in a multiplication-survival trade-off regulated by RpoS. The contribution of trade-off characterist...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2015.12.001
更新日期：2016-04-01 00:00:00
abstract：:Sequences of the most conserved proteins encoded by transposable (pro)phages were used to search recently sequenced Firmicute genomes for candidate transposable prophages. One complete Mu-like prophage, SglyMu-1, was identified, in four copies, in the Syntrophobotulus glycolicus DSM 8271 chromosome sequence. Related p...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.01.008
更新日期：2013-05-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80053-9
更新日期：1999-05-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(94)90182-1
更新日期：1994-05-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.06.004
更新日期：2006-11-01 00:00:00
abstract：:Members of the genus Cronobacter are responsible for cases of meningitis and bacteremia with high fatality rates in neonates. Macrophage uptake of invading microbes is an innate process, and it has been proposed that macrophage infectivity potentiator (Mip) like proteins enhance the ability of pathogens to survive wit...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2015.02.005
更新日期：2015-04-01 00:00:00
abstract：:Cell death induced by adherence of Staphylococcus aureus was observed in Cryptococcus neoformans. To investigate this death mechanism, we examined three factors: actin dynamics using rhodamine-phalloidin staining, accumulation of reactive oxygen species (ROS) via conversion of 2',7'-dichlorofluorescein diacetate to 2'...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.07.006
更新日期：2008-11-01 00:00:00
abstract：:Macroscopic and microscopic measurements of the growth of Trichoderma viride and Rhizopus oligosporus were compared at different values of water activity of the solid medium. The relationship between radial extension rate and hyphal growth rate was found to be dependent on water activity. The observed difference betwe...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90106-x
更新日期：1992-10-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)90130-x
更新日期：2000-10-01 00:00:00
abstract：:During the past twenty-nine years, not a single class of antimicrobial agents has been discovered that has led to new approved human drugs. Despite a dramatic increase in the potency of existing classes, the need for new effective antimicrobial agents continues. The bacterial phosphotransferase system (PTS) offers the...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(92)90089-7
更新日期：1992-06-01 00:00:00
abstract：:Random amplified polymorphic DNA (RAPD) analysis was applied to genomic DNA from nineteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces. Results obtained with five primers indicated that this technique is a powerful tool for yeast differentiation and identification. The data were consistent...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)80565-1
更新日期：1995-09-01 00:00:00
abstract：:(51)V NMR spectroscopy was used for detection and identification of cell-associated vanadate (V(5+)) species after exposure of Phycomyces blakesleeanus mycelium, in exponential phase of growth, to sodium orthovanadate. Complete disappearance of monomer and dimer signals and decreased intensity of the tetramer signal w...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.04.012
更新日期：2016-07-01 00:00:00
abstract：:FliA is known to be a sigma factor that regulates bacterial flagella gene expression. Accumulating evidence suggests that FliA is involved in bacterial behavior other than motility. To elucidate the contribution of FliA to Pseudomonas aeruginosa pathophysiology, we analyzed the biological properties and gene expressio...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2018.02.001
更新日期：2018-04-01 00:00:00
abstract：:Tellurite-containing media are widely used for the screening and isolation of Shiga toxin-producing Escherichia coli (STEC) O157:H7, but tellurite resistance among non-O157 STEC is poorly characterized. Therefore, we investigated 202 STEC strains representing 61 different serotypes from humans, animals or food for the...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.10.007
更新日期：2007-03-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(01)01226-8
更新日期：2001-07-01 00:00:00
abstract：:Ninety-nine strains of Gram-negative black-pigmented anaerobic rods, grown on Todd-Hewitt blood agar plates, were identified and characterized according to a typing scheme including UV fluorescence, catalase, trypsin-like and haemagglutinating activities, biochemical tests with the ATB 32A kit, and gas-liquid chromato...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(93)90051-3
更新日期：1993-07-01 00:00:00
abstract：:The adsorption complex of filamentous phage fd consists of two minor coat proteins, g3p and g6p, and is considered to be not only a structural entity, but also a functional unit to terminate phage assembly. Cells were infected with phage M13am8H1, which cannot assemble because it lacks the major coat protein g8p, alth...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(94)90042-6
更新日期：1994-11-01 00:00:00
abstract：:Mercury methylation and demethylation processes govern the fate of methylmercury in aquatic ecosystems. Under anoxic conditions, methylation activity is mainly of biological origin and is often the result of sulfate-reducing bacteria. In this study, the use of a luminescent biosensor for screening methylmercury produc...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2017.09.005
更新日期：2018-01-01 00:00:00
abstract：:Although chromosomal replication is an essential feature of the bacterial life cycle, the replication mechanism and involved molecular players have never been properly characterized in the Acetobacter genera. Thanks to whole-genome sequencing, the unknown replication proteins from Acetobacter pasteurianus and Acetobac...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.06.010
更新日期：2016-10-01 00:00:00
abstract：:As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revea...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.11.002
更新日期：2009-03-01 00:00:00
abstract：:Legume-nodulating rhizobia use N-acyl homoserine lactones (AHLs) to regulate several physiological traits related to the symbiotic plant-microbe interaction. In this work, we show that Sinorhizobium fredii SMH12, Rhizobium etli ISP42 and Rhizobium sullae IS123, three rhizobial strains with different nodulation ranges,...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.05.002
更新日期：2011-09-01 00:00:00
abstract：:Deinococcus radiodurans is a bacterium best known for its extreme resistance to high levels of ionizing radiation. Gene expression profiles of D. radiodurans exposed to 0.3 M NaCl revealed that at least 389 genes were induced and 415 were repressed by twofold or more. A general down-regulation of the central metabolic...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.07.005
更新日期：2013-11-01 00:00:00
abstract：:Soil is one of the most complex and challenging environments for microbiologists. In fact, although it contains the largest microbial diversity on the planet, the majority of these microbes are still uncharacterized and represent an enormous unexplored reservoir of genetic and metabolic diversity. Metagenomics, the st...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2010.04.010
更新日期：2010-07-01 00:00:00
abstract：:The kinetics of colicin N binding to the Escherichia coli surface, comprising the recognition of and association with cell-surface-exposed sites of OmpF porin, is a rapid event taking place during the first seconds of incubation. Immunogold labelling demonstrates the membrane localization of the colicin N bound to sen...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80012-6
更新日期：1998-10-01 00:00:00
