Colicin N interaction with sensitive Escherichia coli cells: in situ and kinetic approaches.

Abstract:

:The kinetics of colicin N binding to the Escherichia coli surface, comprising the recognition of and association with cell-surface-exposed sites of OmpF porin, is a rapid event taking place during the first seconds of incubation. Immunogold labelling demonstrates the membrane localization of the colicin N bound to sensitive cells. Analyses of colicin-induced efflux indicate a short lag before the onset of cytoplasmic K+ release. This delay reflects the time necessary for translocation from the external side and pore-forming insertion into the cytoplasmic membrane.

journal_name

Res Microbiol

journal_title

Research in microbiology

authors

el Kouhen R,Bernadac A,Pagès JM

doi

10.1016/s0923-2508(99)80012-6

subject

Has Abstract

pub_date

1998-10-01 00:00:00

pages

645-51

issue

9

eissn

0923-2508

issn

1769-7123

pii

S0923-2508(99)80012-6

journal_volume

149

pub_type

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