Abstract:
:The effects of tunicamycin, an inhibitor of N-asparagine-linked glycosylation, on the secretion, adhesion and activities of the cellulase complex produced by Clostridium cellulolyticum have been studied. Tunicamycin at 0.1 micrograms/ml slightly inhibited growth on cellobiose. Endoglucanase, p-nitrophenylcellobiosidase and avicelase activities of the "Avicel"-adsorbed fraction from a culture grown with this drug were decreased 4.4-, 1.4- and 12.2-fold, respectively. During growth on cellulose, tunicamycin considerably inhibited growth and adhesion of cells on their substrate (only 28% of the cells were bound to cellulose). SDS-PAGE mobilities of some proteins excreted during growth with the drug were different from those of proteins from control cultures; the native Avicel-adsorbed fraction (PH2O) consisted of three major components of molecular weights about 135, 90 and 68 kDa, whereas in the presence of tunicamycin (0.1 micrograms/ml), the Avicel-adsorbed fraction (PH2OT) contained only a major band of 105 kDa, and the proteins of 135 and 68 kDa appeared weakly. By using the "Dig Glycan Detection" kit, some proteins appeared to be glycosylated, such as the 135-, 95-, 47- and 40-kDa proteins. Moreover, the affinity for Avicel and the avicelase activity decreased dramatically for the Avicel-adsorbed fraction from a culture grown with the drug. The remaining avicelase activity of the PH2O fraction in the presence of specific P135 antiserum was 50% of the initial activity, whereas CMCase and pNPCbase were not affected. The glycosylated protein of 135 kDa played a prominent role in the adhesion and avicelase activity of C. cellulolyticum. Moreover, the endoglucanase activity in a culture broth from tunicamycin-grown cells was more thermolabile and protease-sensitive than that from control cultures.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Gehin A,Petitdemange Hdoi
10.1016/0923-2508(96)80281-6subject
Has Abstractpub_date
1995-03-01 00:00:00pages
251-62issue
3eissn
0923-2508issn
1769-7123pii
0923-2508(96)80281-6journal_volume
146pub_type
杂志文章abstract::At the beginning of the 20th century, it was discovered at the Pasteur Institute in Tunis that epidemic typhus is transmitted by the human body louse. The complete genome sequence of its causative agent, Rickettsia prowazekii, was determined at Uppsala University in Sweden at the end of the century. In this mini-revie...
journal_title:Research in microbiology
pub_type: 传,历史文章,杂志文章,评审
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abstract::Campylobacter spp. is the most common cause of bacterial diarrhoea in humans worldwide. Therefore, rapid and reliable methods for detection and quantification of this pathogen are required. In this study, we have developed a reverse transcription quantitative real-time PCR (RT-qPCR) for detection and quantification of...
journal_title:Research in microbiology
pub_type: 杂志文章
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abstract::Measuring gene expression at the single cell and single molecule level has recently made possible the quantitative measurement of stochasticity of gene expression. This enables identification of the probable sources and roles of noise. Gene expression noise can result in bacterial population heterogeneity, offering sp...
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journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/S0923-2508(02)00013-X
更新日期:2003-03-01 00:00:00
abstract::Macroscopic and microscopic measurements of the growth of Trichoderma viride and Rhizopus oligosporus were compared at different values of water activity of the solid medium. The relationship between radial extension rate and hyphal growth rate was found to be dependent on water activity. The observed difference betwe...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(92)90106-x
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abstract::General and regulated proteolysis in bacteria is crucial for cellular homeostasis and relies on high substrate specificity of the executing AAA+ proteases. Here we summarize the various strategies that tightly control substrate degradation from both sides: the generation of accessible degrons and their specific recogn...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2009.08.018
更新日期:2009-11-01 00:00:00
abstract::Development of effective strategies for treatment of candidiasis and other fungal diseases has posed a challenge, considering the increase in opportunistic fungal infections in HIV-positive and immunocompromised patients. The in vitro antifungal activity of essential oil from Ocimum gratissimum was investigated in ord...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.04.004
更新日期:2004-09-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/S0923-2508(03)00148-7
更新日期:2003-10-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(00)90130-x
更新日期:2000-10-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2012.08.001
更新日期:2012-09-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.10.009
更新日期:2005-04-01 00:00:00
abstract::Randomly cloned fragments (in a size range 1 to 2.5 kb) of DNA from Bifidobacterium longum ATCC 15707, B. adolescentis CIP 64.59T, B. bifidum CIP 64.65 and B. animalis ATCC 25527 were used as hybridization probes to characterize strains of these species and distinguish them from closely related Bifidobacterium species...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)80271-3
更新日期:1995-01-01 00:00:00
abstract::The [URE3] element of the yeast Saccharomyces cerevisiae results from the presence of an altered form of the nitrogen regulatory protein Ure2. This altered form acts as an infectious protein (prion). Genes affecting [URE3] initiation and propagation should give valuable information about prion diseases as well as othe...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(01)01239-6
更新日期:2001-09-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.09.013
更新日期:2009-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.resmic.2004.11.001
更新日期:2005-04-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2018.02.001
更新日期:2018-04-01 00:00:00
abstract::Mycotoxins are toxic secondary metabolites produced by filamentous fungi that have been detected in food commodities, including grapes and wine. A survey was conducted to assess mycotoxin-producing fungi in grapes destined for wine production. The mycotoxigenic capacity of the isolates was tested in culture media. Gra...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.12.005
更新日期:2005-05-01 00:00:00
abstract::The major 25-kDa outer membrane protein (Omp25) of Brucella abortus was purified and antigenically characterized by use of monoclonal antibodies (mAbs). Purification was achieved from the sodium dodecyl sulphate-insoluble (SDS-I) cell wall (CW) fraction of vaccine strain B. abortus B19 which was shown by use of mAbs t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)81383-0
更新日期:1996-05-01 00:00:00
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journal_title:Research in microbiology
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doi:10.1016/j.resmic.2010.05.015
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pub_type: 杂志文章,评审
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更新日期:1994-05-01 00:00:00
abstract::A bacteriolytic enzyme excreted by Pseudomonas aeruginosa Paks I was purified: samples were found to be homogeneous by gel filtration chromatography, ion exchange chromatography using CM-cellulose, immunoelectrophoresis, PAGE and SDS-PAGE. The molecular weight of the lytic enzyme was estimated to be 15,000-19,000. The...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90046-6
更新日期:1989-02-01 00:00:00
abstract::The sequence of a 4.6-kb region of DNA from Corynebacterium glutamicum ATCC 13869 lying upstream from the ftsQ-ftsZ region has been determined. The region contains four genes with high similarity to the murD, ftsW, murG, and murC genes from different microorganisms. The products of these mur genes probably catalyse se...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2003.11.003
更新日期:2004-04-01 00:00:00
abstract::In this study, we identified an antimicrobial compound produced by the Gram-negative bacterium Serratia marcescens. Colonies of S. marcescens inhibited the growth of nine different methicillin-resistant Staphylococcus aureus (MRSA) isolates and several other tested Gram-positive bacterial species, but not Gram-negativ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.06.002
更新日期:2013-10-01 00:00:00
abstract::Benzene is biodegraded in the absence of oxygen under a variety of terminal electron-accepting conditions. However, the mechanism by which anaerobic benzene degradation occurs is unclear. Phenol and benzoate have been consistently detected as intermediates of anaerobic benzene degradation, suggesting that the hydroxyl...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(02)01378-5
更新日期:2002-12-01 00:00:00
abstract::The removal of water through air drying damages membranes, proteins and nucleic acids and is lethal to the majority of organisms. Nevertheless, some vegetative cells of bacteria and cyanobacteria survive extreme desiccation. Understanding the mechanisms of their desiccation tolerance is an important issue in cell biol...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(01)01279-7
更新日期:2002-01-01 00:00:00
abstract::As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revea...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2008.11.002
更新日期:2009-03-01 00:00:00
abstract::An unusual structural component of plasmid pUC18 was observed in transformants of Escherichia coli strains. This molecule migrated faster than superhelical topoisomers in agarose gel electrophoresis and was difficult to stain with ethidium bromide. This unusual topology appeared independently of gyrase mutation, and t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(92)90061-r
更新日期:1992-09-01 00:00:00
abstract::DNA-DNA hybridization showed that the Brucella strains recently isolated from marine mammals belong to the monospecific genus Brucella (more than 77% DNA relatedness). Ribotyping (HindIII rDNA restriction patterns) showed that they may represent a separate subgroup (marine type) specifically associated with marine mam...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(00)01145-1
更新日期:2000-11-01 00:00:00
abstract::Lectin-like substances of 54 marine algae, corresponding to 30 red algae and 24 brown algae, were studied to estimate the agglutination activity against Candida guilliermondii. Extracts of Dictyopteris membranacea, Bifurcaria bifurcata, Halidrys siliquosa, Ascophyllum nodosum, Fucus serratus and F. spiralis algae show...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90013-2
更新日期:1989-07-01 00:00:00