Molecular genetic characterisation of whiB3, a mycobacterial homologue of a Streptomyces sporulation factor.

abstract：:This communication describes the cloning of a 1.8-kb fragment from the genome of the corynephage phi AAU2, which aborts the phage lytic cycle when cloned on a high-copy shuttle vector. The associated phenotype, called Apld (aborting phage lytic development), was revealed by noting the reduced plaque size and lower eff...

journal_title：Research in microbiology

authors： Le Marrec C,Moreau S,Leret V,Schäffer A,Blanco C,Trautwetter A

更新日期：1998-03-01 00:00:00

abstract：:Vegetative incompatibility limits heterokaryon formation in fungi. It results from genetic differences at specific loci (het loci). Characterization of het genes and, more recently, of incompatibility reaction suppressors has, provided insight into the mechanisms involved. A link between development, vegetative incomp...

journal_title：Research in microbiology

authors： Loubradou G,Turcq B

更新日期：2000-05-01 00:00:00

abstract：:Amplified fragment length polymorphism (AFLP), a novel method for molecular typing, was evaluated for its ability to differentiate among a group of highly related Chlamydia psittaci strains isolated from ruminants and belonging to serotype 1. A total set of 12 strains were included in this study, 10 strains inducing a...

journal_title：Research in microbiology

authors： Boumedine KS,Rodolakis A

更新日期：1998-11-01 00:00:00

abstract：:A new actinomycete strain designated US24 producing antibacterial activities against Gram-positive and Gram-negative bacteria was isolated from Tunisian soil. Culture characteristic studies strongly suggested that the US24 strain belonged to the genus Streptomyces. Analysis of the nucleotide sequence of the 16S rRNA g...

journal_title：Research in microbiology

authors： Mellouli L,Ben Ameur-Mehdi R,Sioud S,Salem M,Bejar S

更新日期：2003-06-01 00:00:00

abstract：:Differential immunoproteomics was applied to identify proteins secreted by Metarhizium anisopliae induced by the Rhipicephalus microplus cuticle. In addition, IgG anti-spore surface proteins were used for searching for proteins possibly involved in early stages of fungus versus tick infection. LC-MS/MS of differential...

journal_title：Research in microbiology

authors： Santi L,Silva WO,Pinto AF,Schrank A,Vainstein MH

更新日期：2009-12-01 00:00:00

abstract：:Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli...

journal_title：Research in microbiology

authors： Chai BF,Wang W,Liang AH

更新日期：2006-04-01 00:00:00

abstract：:Riemerella anatipestifer (RA) causes major economic losses to the duck industry. Autoinducer-2 (AI-2) is a quorum-sensing signal that regulates bacterial physiology. The luxS and pfs genes are required for AI-2 synthesis in many bacterial species. pfs encodes Pfs, which functions upstream of LuxS in the biosynthesis o...

journal_title：Research in microbiology

authors： Han X,Liu L,Fan G,Zhang Y,Xu D,Zuo J,Wang S,Wang X,Tian M,Ding C,Yu S

更新日期：2015-07-01 00:00:00

abstract：:Monopolization of the vaginal econiche by a limited number of Lactobacillus species, resulting in low pH of 3.5-4.5, has been shown to protect women against vaginal dysbiosis, sexually transmitted infections and adverse pregnancy outcomes. Still, controversy exists as to which characteristics of lactobacilli are most ...

journal_title：Research in microbiology

authors： Vaneechoutte M

更新日期：2017-01-01 00:00:00

abstract：:The work described here continues our studies of the effects of subinhibitory concentrations of antibiotics on SOS and DNA repair gene expression in Staphylococcus aureus. Mitomycin C and the new-generation fluoroquinolone moxifloxacin induced expression of SOS response genes (lexA, recA, sosA, and umuC) in a ciproflo...

journal_title：Research in microbiology

authors： Mesak LR,Davies J

更新日期：2009-12-01 00:00:00

abstract：:Metagenomic libraries represent subsamples of the total DNA found at a study site and offer unprecedented opportunities to study ecological and functional aspects of microbial communities. To examine the depth of a community sequencing effort, rarefaction analysis of the ribosomal small subunit (SSU/16S/18S) gene in t...

journal_title：Research in microbiology

authors： Bengtsson J,Hartmann M,Unterseher M,Vaishampayan P,Abarenkov K,Durso L,Bik EM,Garey JR,Eriksson KM,Nilsson RH

更新日期：2012-07-01 00:00:00

abstract：:Plasmids that express an enzymatically active beta-galactosidase in an Escherichia coli delta lac strain were isolated from libraries of recombinant plasmids containing Vibrio cholerae chromosomal DNA. Deletion analysis localized the gene responsible for beta-galactosidase activity on a 3.1-kb DNA fragment, and the ge...

journal_title：Research in microbiology

authors： Parsot C

更新日期：1992-01-01 00:00:00

abstract：:Mrd1 is one of the trans-acting proteins and plays an important role in precursor ribosomal RNA processing. Here we characterized the Mamrd1 gene from Metarhizium acridum and studied its function in growth, conidiation and virulence using RNA interference. The Mamrd1 gene was identified as participating in the process...

journal_title：Research in microbiology

authors： Cao Y,Li K,Xia Y

更新日期：2011-09-01 00:00:00

abstract：:The catabolite repressor-activator (Cra) protein controls the direction of carbon flux through metabolic pathways in enteric bacteria. Cra binds to the control regions of target genes and exerts a negative effect on the expression of genes encoding glycolytic and Entner-Doudoroff enzymes, while exerting a positive eff...

journal_title：Research in microbiology

authors： Ramseier TM

更新日期：1996-07-01 00:00:00

abstract：:Rhodococcus strains are important biocatalysts used for biotechnological production of acrylamide catalysed by a nitrile hydratase (NHase) containing cobalt. This metalloenzyme is present at high intracellular concentrations representing up to 50% of the soluble proteins in Rhodococcus rhodochrous M8 strain. Cobalt io...

journal_title：Research in microbiology

authors： Lavrov KV,Shemyakina AO,Grechishnikova EG,Novikov AD,Derbikov DD,Kalinina TI,Yanenko AS

更新日期：2018-01-01 00:00:00

abstract：:Magnetotactic bacteria (MTB) are a group of Gram-negative bacteria synthesizing magnetic crystals that allow them aligning along magnetic field lines. They have diverse morphologies including cocci, rods, vibrio, spirilla, and multicellular magnetotactic prokaryotes (MMPs). MMPs are composed of 10-57 cells with peritr...

journal_title：Research in microbiology

authors： Zhang R,Chen YR,Du HJ,Zhang WY,Pan HM,Xiao T,Wu LF

更新日期：2014-09-01 00:00:00

abstract：:Campylobacter spp. is the most common cause of bacterial diarrhoea in humans worldwide. Therefore, rapid and reliable methods for detection and quantification of this pathogen are required. In this study, we have developed a reverse transcription quantitative real-time PCR (RT-qPCR) for detection and quantification of...

journal_title：Research in microbiology

authors： Bui XT,Wolff A,Madsen M,Bang DD

更新日期：2012-01-01 00:00:00

abstract：:A Gram-negative, short rod, aerobic bacterium, designated W11(T), was isolated from seawater. Heterotrophic growth was observed at 10-45 °C and pH 6-10. Optimal growth was observed at 30-37 °C and pH 7-9. It can grow in the presence of 0.5-12% NaCl (w/v), and the optimal NaCl required for growth was 5-6%. 16S rRNA gen...

journal_title：Research in microbiology

authors： Poddar A,Lepcha RT,Mukherjee D,Bhattacharyya D,Das SK

更新日期：2014-09-01 00:00:00

abstract：:Haemophilus parainfluenzae and Haemophilus influenzae, upper respiratory tract microbiota representatives, are able to colonize natural and artificial surfaces as biofilm. The aim of the present study was to assay the effect of ten 1,2,4-triazole-ciprofloxacin hybrids on planktonic or biofilm-forming haemophili cells ...

journal_title：Research in microbiology

authors： Kosikowska U,Andrzejczuk S,Plech T,Malm A

更新日期：2016-10-01 00:00:00

abstract：:The neurotoxin gene from a strain of Clostridium botulinum type A causing infant botulism was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated using primers designed to conserved regions of published botulinal toxin (BoNT) sequences. Translation of the nucleotide sequence derived f...

journal_title：Research in microbiology

authors： Willems A,East AK,Lawson PA,Collins MD

更新日期：1993-09-01 00:00:00

abstract：:Streptococcus intermedius, an oral commensal and a cause of systemic pyogenic disease, expresses fimbriae. To identify the gene(s) encoding these fimbriae, we used a serum raised against purified fimbriae to screen libaries of recombinant lambda phages. The cloned gene cluster encoding S. intermedius fimbriae, (saliva...

journal_title：Research in microbiology

authors： Yamaguchi T,Matsumoto M,Sugimoto Y,Soutome S,Oho T

更新日期：2009-12-01 00:00:00

abstract：:A highly effective chlorpyrifos-degrading bacterium strain Dsp-2 was isolated from the polluted treatment system of a chlorpyrifos manufacturer. This strain was preliminarily identified as Sphingomonas sp. based on its morphological, physiological and biochemical tests as well as 16S rDNA analysis. It utilized chlorpy...

journal_title：Research in microbiology

authors： Li X,He J,Li S

更新日期：2007-03-01 00:00:00

abstract：:Eight strains of acidophilic bacteria, isolated from mine-impacted and geothermal sites from different parts of the world, were shown to form a distinct clade (proposed genus "Acidibacillus") within the phylum Firmicutes, well separated from the acidophilic genera Sulfobacillus and Alicyclobacillus. Two of the strains...

journal_title：Research in microbiology

authors： Holanda R,Hedrich S,Ňancucheo I,Oliveira G,Grail BM,Johnson DB

更新日期：2016-09-01 00:00:00

abstract：:Growth of fluoroquinolone (FQ)-susceptible methicillin-resistant Staphylococcus aureus (MRSA) in the presence of sub-MIC FQ has been shown to enhance methicillin resistance in traditional nosocomial MRSA isolates. We aimed to confirm this phenomenon in nine community-associated MRSA (CA-MRSA) clinical isolates, and to...

journal_title：Research in microbiology

authors： Tattevin P,Basuino L,Chambers HF

更新日期：2009-04-01 00:00:00

abstract：:The mechanism by which TrwB acts as a DNA transporter in bacterial conjugation is analyzed. Based on a parallelism between TrwB and F(1)-ATPase, TrwB would use the energy derived from ATP hydrolysis to pump DNA through its central channel, in a manner similar to that used by F(1)-ATPase to produce a rotary movement of...

journal_title：Research in microbiology

authors： Cabezon E,de la Cruz F

更新日期：2006-05-01 00:00:00

abstract：:The diversity and temporal dynamics of bacterial communities in pristine snow around two Russian Antarctic stations was investigated. Taxonomic analysis of rDNA libraries revealed that snow communities were dominated by bacteria from a small number of operational taxonomic units (OTUs) that underwent dramatic swings i...

journal_title：Research in microbiology

authors： Lopatina A,Krylenkov V,Severinov K

更新日期：2013-11-01 00:00:00

abstract：:Subtractive differential hybridization was used to identify genes in Bacillus subtilis that are induced by nutrient limitation. Several transcription units were identified. They exhibited increased transcription when cells were deprived of certain nutrients, such as glucose, ammonium, or phosphate, or when cells were ...

journal_title：Research in microbiology

authors： Mueller JP,Mathiopoulos C,Slack FJ,Sonenshein AL

更新日期：1991-09-01 00:00:00

abstract：:Viruses play important roles in aquatic ecosystems, but deep-sea bacteriophages remain largely unexplored. A temperate bacteriophage (termed vB_PstS-1) was identified from the psychrotolerant gammaproteobacterium Pseudomonas stutzeri 1-1-1b, which was isolated from hadopelagic water (depth of 7000 m) of the Japan Tren...

journal_title：Research in microbiology

authors： Yoshida M,Yoshida-Takashima Y,Nunoura T,Takai K

更新日期：2015-11-01 00:00:00

abstract：:DNA-DNA hybridization showed that the Brucella strains recently isolated from marine mammals belong to the monospecific genus Brucella (more than 77% DNA relatedness). Ribotyping (HindIII rDNA restriction patterns) showed that they may represent a separate subgroup (marine type) specifically associated with marine mam...

journal_title：Research in microbiology

authors： Verger JM,Grayon M,Cloeckaert A,Lefèvre M,Ageron E,Grimont F

更新日期：2000-11-01 00:00:00

abstract：:KlADH4 is a gene of Kluyveromyces lactis encoding a mitochondrial alcohol dehydrogenase activity, which is specifically induced by ethanol and insensitive to glucose repression. In this work, we report the molecular analysis of UAS(E), an element of the KlADH4 promoter which is essential for the induction of KlADH4 in...

journal_title：Research in microbiology

authors： Mazzoni C,Santori F,Saliola M,Falcone C

更新日期：2000-01-01 00:00:00

abstract：:Most recently published complete bacterial genomes have revealed unexpectedly high numbers of long strict repeats. In this article we discuss the various functional and evolutionary roles of these repeats, focusing in particular on their role in terms of genome stability, gene transfer, and antigenic variation. ...

journal_title：Research in microbiology

authors： Rocha EP,Danchin A,Viari A

更新日期：1999-11-01 00:00:00