DNA fingerprinting by random amplified polymorphic DNA and restriction fragment length polymorphism is useful for yeast typing.

abstract：:The trehalose operon of Pseudomonas fluorescens ATCC 17400 consists of treP, treA and treR. The gene treP codes for a putative enzyme II subunit of the phosphotransferase system that catalyzes the phosphorylation of trehalose together with its translocation across the cell membrane and treA encodes a putative phosphot...

journal_title：Research in microbiology

authors： Matthijs S,Koedam N,Cornelis P,De Greve H

更新日期：2000-12-01 00:00:00

abstract：:In recent years there has been a resurgence of research to develop new and improved attenuated strains of Salmonella typhi to function as live oral vaccines against typhoid fever and to serve as "carrier" vaccines to express foreign antigens of other pathogens and deliver them to the immune system. Strain Ty21a has se...

journal_title：Research in microbiology

authors： Levine MM,Hone D,Tacket C,Ferreccio C,Cryz S

更新日期：1990-09-01 00:00:00

abstract：:Viruses play important roles in aquatic ecosystems, but deep-sea bacteriophages remain largely unexplored. A temperate bacteriophage (termed vB_PstS-1) was identified from the psychrotolerant gammaproteobacterium Pseudomonas stutzeri 1-1-1b, which was isolated from hadopelagic water (depth of 7000 m) of the Japan Tren...

journal_title：Research in microbiology

authors： Yoshida M,Yoshida-Takashima Y,Nunoura T,Takai K

更新日期：2015-11-01 00:00:00

abstract：:Cell-free extracts of various strains belonging to Mycobacterium paratuberculosis (Ptb) and "wood-pigeon" (WP) mycobacteria were compared by polyacrylamide gel electrophoresis and the various protein bands obtained were tested for peroxidase enzyme activity. One strain of Mycobacterium avium served as a control. Bacte...

journal_title：Research in microbiology

authors： Thorel MF,Blom-Potar MC,Rastogi N

更新日期：1990-06-01 00:00:00

abstract：:Chinoike Jigoku ("Blood Pond Hell") is located in the hot spring town of Beppu on the southern island of Kyushu in Japan, and is the site of a red-colored acidic geothermal pond. This study aimed to investigate the microbial population composition in this extremely acidic environment and to isolate/characterize acidop...

journal_title：Research in microbiology

authors： Masaki Y,Tsutsumi K,Hirano S,Okibe N

更新日期：2016-09-01 00:00:00

abstract：:Ribosomal DNA (rDNA) polymorphism was compared to enzyme polymorphism and DNA/DNA hybridization data for the intraspecies differentiation of Providencia alcalifaciens. DNA from 27 strains previously classified into two zymotypes A1 and A2 and discriminated by two levels of DNA/DNA hybridization (delta Tm values of 0 t...

journal_title：Research in microbiology

authors： Picard B,Picard-Pasquier N,Krishnamoorthy R,Goullet P

更新日期：1991-11-01 00:00:00

abstract：:The effects of tunicamycin, an inhibitor of N-asparagine-linked glycosylation, on the secretion, adhesion and activities of the cellulase complex produced by Clostridium cellulolyticum have been studied. Tunicamycin at 0.1 micrograms/ml slightly inhibited growth on cellobiose. Endoglucanase, p-nitrophenylcellobiosidas...

journal_title：Research in microbiology

authors： Gehin A,Petitdemange H

更新日期：1995-03-01 00:00:00

abstract：:Eight strains of acidophilic bacteria, isolated from mine-impacted and geothermal sites from different parts of the world, were shown to form a distinct clade (proposed genus "Acidibacillus") within the phylum Firmicutes, well separated from the acidophilic genera Sulfobacillus and Alicyclobacillus. Two of the strains...

journal_title：Research in microbiology

authors： Holanda R,Hedrich S,Ňancucheo I,Oliveira G,Grail BM,Johnson DB

更新日期：2016-09-01 00:00:00

abstract：:In this study, we identified an antimicrobial compound produced by the Gram-negative bacterium Serratia marcescens. Colonies of S. marcescens inhibited the growth of nine different methicillin-resistant Staphylococcus aureus (MRSA) isolates and several other tested Gram-positive bacterial species, but not Gram-negativ...

journal_title：Research in microbiology

authors： Kadouri DE,Shanks RM

更新日期：2013-10-01 00:00:00

abstract：:Growth of fluoroquinolone (FQ)-susceptible methicillin-resistant Staphylococcus aureus (MRSA) in the presence of sub-MIC FQ has been shown to enhance methicillin resistance in traditional nosocomial MRSA isolates. We aimed to confirm this phenomenon in nine community-associated MRSA (CA-MRSA) clinical isolates, and to...

journal_title：Research in microbiology

authors： Tattevin P,Basuino L,Chambers HF

更新日期：2009-04-01 00:00:00

abstract：:A novel acidophilic member of the phylum Actinobacteria was isolated from an acidic stream draining an abandoned copper mine in north Wales. The isolate (PY-F3) was demonstrated to be a heterotroph that catalyzed the oxidation of ferrous iron (but not of sulfur or hydrogen) under aerobic conditions, and the reduction ...

journal_title：Research in microbiology

authors： Jones RM,Johnson DB

更新日期：2015-02-01 00:00:00

abstract：:Bacteria of the Bacillus cereus group are environmental Gram-positive spore-forming bacteria ubiquitously distributed. Despite the high degree of genetic similarity among the different strains, they show strong phenotypic variability, from mammal or entomopathogen strains to soil-dwelling saprophytes, and from psychro...

journal_title：Research in microbiology

authors： Patiño-Navarrete R,Sanchis V

更新日期：2017-05-01 00:00:00

abstract：:Metagenomic libraries represent subsamples of the total DNA found at a study site and offer unprecedented opportunities to study ecological and functional aspects of microbial communities. To examine the depth of a community sequencing effort, rarefaction analysis of the ribosomal small subunit (SSU/16S/18S) gene in t...

journal_title：Research in microbiology

authors： Bengtsson J,Hartmann M,Unterseher M,Vaishampayan P,Abarenkov K,Durso L,Bik EM,Garey JR,Eriksson KM,Nilsson RH

更新日期：2012-07-01 00:00:00

abstract：:The removal of water through air drying damages membranes, proteins and nucleic acids and is lethal to the majority of organisms. Nevertheless, some vegetative cells of bacteria and cyanobacteria survive extreme desiccation. Understanding the mechanisms of their desiccation tolerance is an important issue in cell biol...

journal_title：Research in microbiology

authors： Billi D,Potts M

更新日期：2002-01-01 00:00:00

abstract：:The human gastric pathogen Helicobacter pylori possesses an enormous genomic plasticity and diversity that facilitates host adaptation. Despite the ancient association with its human host, this epsilon-proteobacterium can cause gastritis, ulcers and gastric cancer. Here we focus on multiple aspects of the genome level...

journal_title：Research in microbiology

authors： Linz B,Schuster SC

更新日期：2007-12-01 00:00:00

abstract：:There is still an urgent requirement for more sensitive, cost-effective methods for detection and susceptibility testing of mycobacteria in clinical samples. We have been investigating a simple bacteriophage-based system which could be used for both purposes. As this depends upon the detection of phages which have suc...

journal_title：Research in microbiology

authors： McNerney R,Wilson SM,Sidhu AM,Harley VS,al Suwaidi Z,Nye PM,Parish T,Stoker NG

更新日期：1998-07-01 00:00:00

abstract：:Several specific primers for the nifH gene were tested with different pure telluric N2-fixing strains. A PolF/PolR primer set provided successful amplification of 19 representative N2-fixing strains. Three restriction enzymes, HaeIII, NdeII and MnlI, chosen for restriction fragment length polymorphism (RFLP) analyses,...

journal_title：Research in microbiology

authors： Poly F,Monrozier LJ,Bally R

更新日期：2001-01-01 00:00:00

abstract：:General and regulated proteolysis in bacteria is crucial for cellular homeostasis and relies on high substrate specificity of the executing AAA+ proteases. Here we summarize the various strategies that tightly control substrate degradation from both sides: the generation of accessible degrons and their specific recogn...

journal_title：Research in microbiology

authors： Schmidt R,Bukau B,Mogk A

更新日期：2009-11-01 00:00:00

abstract：:The insertion of proteins into membranes generally requires the assistance of membrane proteins. A protein, designated Omp85 in Neisseria meningitidis, was shown to be required for the assembly of bacterial outer-membrane proteins. The protein is essential for the viability of the bacteria and is ubiquitous among Gram...

journal_title：Research in microbiology

authors： Voulhoux R,Tommassen J

更新日期：2004-04-01 00:00:00

abstract：:Evidence of increasing resistance to antibiotics in soil and other natural isolates highlights the importance of horizontal transfer of resistance genes in facilitating gene flux in bacteria. Horizontal gene transfer in bacteria is favored by the presence of mobile genetic elements and by the organization of bacterial...

journal_title：Research in microbiology

authors： Nwosu VC

更新日期：2001-06-01 00:00:00

abstract：:Pseudomonas mendocina carrying a novel class 1 integron containing an IMP-8 gene was isolated from an inanimate surface in a female ward sanitary facility of the Hospital Infante D. Pedro, Aveiro, Portugal. Hybridization with the integrase gene (intI1) and 16S rDNA revealed that the integron is chromosomally located. ...

journal_title：Research in microbiology

authors： Santos C,Caetano T,Ferreira S,Mendo S

更新日期：2010-05-01 00:00:00

abstract：:The major 25-kDa outer membrane protein (Omp25) of Brucella abortus was purified and antigenically characterized by use of monoclonal antibodies (mAbs). Purification was achieved from the sodium dodecyl sulphate-insoluble (SDS-I) cell wall (CW) fraction of vaccine strain B. abortus B19 which was shown by use of mAbs t...

journal_title：Research in microbiology

authors： Cloeckaert A,Zygmunt MS,Bézard G,Dubray G

更新日期：1996-05-01 00:00:00

abstract：:Subtractive differential hybridization was used to identify genes in Bacillus subtilis that are induced by nutrient limitation. Several transcription units were identified. They exhibited increased transcription when cells were deprived of certain nutrients, such as glucose, ammonium, or phosphate, or when cells were ...

journal_title：Research in microbiology

authors： Mueller JP,Mathiopoulos C,Slack FJ,Sonenshein AL

更新日期：1991-09-01 00:00:00

abstract：:The structure of DspF, a type III secretion system (T3SS) chaperone required for virulence of the fruit tree pathogen Erwinia amylovora, was modeled based on predicted structural homology to characterized T3SS chaperones. This model guided the selection of 11 amino acid residues that were individually mutated to alani...

journal_title：Research in microbiology

authors： Triplett LR,Wedemeyer WJ,Sundin GW

更新日期：2010-09-01 00:00:00

abstract：:We showed in 1988 that there are two strains of Chlamydia psittaci which infect the koala (Phascolarctos cinereus). In order to further investigate the role of these chlamydial strains in pathogenesis, we have attempted to identify genes of koala type I strain chlamydia which are involved in the immunogenic response. ...

journal_title：Research in microbiology

authors： Girjes AA,Carrick FN,Lavin MF

更新日期：1997-06-01 00:00:00

abstract：:Although great strides have been made in the development of recombinant attenuated Vibrio cholerae vaccine strains, the task has not been as simple as once imagined. The initial vaccine candidates proved to be unexpectedly reactogenic but further derivatives, such as CVD103-HgR, are well-tolerated, immunogenic and pro...

journal_title：Research in microbiology

authors： Kaper JB,Levine MM

更新日期：1990-09-01 00:00:00

abstract：:Association with a surface in a structure known as biofilm is the prevailing microbial lifestyle. Here we show the kinetics of biofilm formation of Escherichia coli W3110 in static cultures growing under aerobic or anaerobic conditions. Aerobically growing cells in LB medium started to produce detectable amounts of bi...

journal_title：Research in microbiology

authors： Colón-González M,Méndez-Ortiz MM,Membrillo-Hernández J

更新日期：2004-09-01 00:00:00

abstract：:Soil is one of the most complex and challenging environments for microbiologists. In fact, although it contains the largest microbial diversity on the planet, the majority of these microbes are still uncharacterized and represent an enormous unexplored reservoir of genetic and metabolic diversity. Metagenomics, the st...

journal_title：Research in microbiology

authors： Mocali S,Benedetti A

更新日期：2010-07-01 00:00:00

abstract：:Genome analyses have revealed that the genomes of non-photosynthetic bacteria including Bacillus subtilis code for proteins similar to the large subunit of RuBisCO (called RuBisCO-like protein (RLP)). This raises a fundamental question as to their functional relationship to photosynthetic RuBisCO. Recently, we identif...

journal_title：Research in microbiology

authors： Ashida H,Danchin A,Yokota A

更新日期：2005-06-01 00:00:00

abstract：:Three primers derived from the lap gene were used to distinguish Listeria monocytogenes from L.innocua and other Listeria species. L. monocytogenes and L. innocua yielded a PCR product of 600 and 300 bp, respectively, whereas a typical pattern of three amplimers was observed with L. ivanovii, L. seeligeri and L. welsh...

journal_title：Research in microbiology

authors： Manzano M,Cocolin L,Ferroni P,Gasparini V,Narduzzi D,Cantoni C,Comi G

更新日期：1996-10-01 00:00:00