Abstract：
:The use of plasmid pKK3535 (a pBR322-derived plasmid containing a ribosomal RNA operon) is described for determining rRNA gene restriction patterns. The plasmid allowed the use of both radioactive as well as nonradioactive procedures and provided features that are necessary for the inclusion of internal and/or external standards that facilitate comparison of strains. The patterns generated are identical to those obtained by hybridization with 32P-labelled rRNA.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Altwegg M,Mayer LWdoi
10.1016/0923-2508(89)90024-7subject
Has Abstractpub_date
1989-05-01 00:00:00pages
325-33issue
4-5eissn
0923-2508issn
1769-7123pii
0923-2508(89)90024-7journal_volume
140pub_type
杂志文章abstract：:OprI, a small outer membrane lipoprotein from Pseudomonas aeruginosa, can be produced in large amounts and anchored at the surface on Escherichia coli cells. A four-time repeated (His-Cys) motif was fused to the C-terminal part of OprI. After induction, E. coli cells harbouring the recombinant oprI gene became more se...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(01)01263-3
更新日期：2001-11-01 00:00:00
abstract：:Production of aerobactin has been reported to be a virulence factor in members of the family Enterobacteriaceae. To investigate the protection afforded by humoral immunity directed towards aerobactin in infectious diseases caused by aerobactin-producing strains, we tested the efficacy of mAbAERO1, a murine monoclonal ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)80894-1
更新日期：1995-02-01 00:00:00
abstract：:The phylogenetic diversity of a sediment microbial community from two ponds having different salinities, 150-200 g/l (M2) and 250-300 g/l (TS38), of an Sfax (Tunisia) solar saltern, was investigated using 16S rRNA clone libraries. The 16S rRNA genes from 135 bacterial clones and 105 archaeal clones were sequenced and ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2010.05.009
更新日期：2010-09-01 00:00:00
abstract：:A variety of pathways for carbon and electron flow in the bacterium Escherichia coli and in other enteric bacteria are differentially expressed depending on whether molecular oxygen is present in the cell environment. This review briefly summarizes the metabolic pathways operative during aerobic versus anaerobic cell ...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(94)90092-2
更新日期：1994-06-01 00:00:00
abstract：:The biological complexity of trade-offs has been a major obstacle in understanding bacterial diversity and coexistence. Here we reduce the biological complexity by using isogenic Escherichia coli strains differing only in a multiplication-survival trade-off regulated by RpoS. The contribution of trade-off characterist...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2015.12.001
更新日期：2016-04-01 00:00:00
abstract：:Four monoclonal antibodies against chlamydial lipopolysaccharide (LPS) were used to study their localization and distribution in the Chlamydia psittaci AB7 abortion-causing strain by immunoelectron microscopy. A non-embedding technique on whole chlamydiae, together with a post-embedding technique on McCoy cells infect...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(94)90078-7
更新日期：1994-10-01 00:00:00
abstract：:A novel acidophilic member of the phylum Actinobacteria was isolated from an acidic stream draining an abandoned copper mine in north Wales. The isolate (PY-F3) was demonstrated to be a heterotroph that catalyzed the oxidation of ferrous iron (but not of sulfur or hydrogen) under aerobic conditions, and the reduction ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2015.01.003
更新日期：2015-02-01 00:00:00
abstract：:Random amplified polymorphic DNA (RAPD) analysis was applied to genomic DNA from nineteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces. Results obtained with five primers indicated that this technique is a powerful tool for yeast differentiation and identification. The data were consistent...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)80565-1
更新日期：1995-09-01 00:00:00
abstract：:Rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. We evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA) as an identification tool for distinguishin...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2012.10.017
更新日期：2013-02-01 00:00:00
abstract：:Lipoic acid is a covalently-bound enzyme cofactor required for central metabolism all three domains of life. In the last 20 years the pathway of lipoic acid synthesis and metabolism has been established in Escherichia coli. Expression of the genes of the lipoic acid biosynthesis pathway was believed to be constitutive...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.04.005
更新日期：2014-07-01 00:00:00
abstract：:Mrd1 is one of the trans-acting proteins and plays an important role in precursor ribosomal RNA processing. Here we characterized the Mamrd1 gene from Metarhizium acridum and studied its function in growth, conidiation and virulence using RNA interference. The Mamrd1 gene was identified as participating in the process...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.05.004
更新日期：2011-09-01 00:00:00
abstract：:FliA is known to be a sigma factor that regulates bacterial flagella gene expression. Accumulating evidence suggests that FliA is involved in bacterial behavior other than motility. To elucidate the contribution of FliA to Pseudomonas aeruginosa pathophysiology, we analyzed the biological properties and gene expressio...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2018.02.001
更新日期：2018-04-01 00:00:00
abstract：:The development of nascent cell division sites was studied in Escherichia coli strains containing ftsAts and ftsZts mutations in which septal development is arrested after shift to the restrictive temperature. Division sites were studied by measuring positions of plasmolysis bays, a visible marker for periseptal annul...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(91)90047-e
更新日期：1991-02-01 00:00:00
abstract：:In this study, we identified an antimicrobial compound produced by the Gram-negative bacterium Serratia marcescens. Colonies of S. marcescens inhibited the growth of nine different methicillin-resistant Staphylococcus aureus (MRSA) isolates and several other tested Gram-positive bacterial species, but not Gram-negativ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.06.002
更新日期：2013-10-01 00:00:00
abstract：:Bacillus species as a group offer several advantages over other bacteria for protection against root pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. The objectives of this work were to determine the ability of strains of Bacillus to inhibit Fusari...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.03.001
更新日期：2005-06-01 00:00:00
abstract：:The endo-N-acetyl-beta-D-glucosaminidases (ENGase) acting on the N-N'-diacetylchitobiosyl core of N-glycosylproteins are essential reagents for the investigation of the structure and the functions of glycoproteins. These enzymes were largely studied with the aim of offering more tools with new and broader substrate sp...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(96)80289-0
更新日期：1995-07-01 00:00:00
abstract：:The trehalose operon of Pseudomonas fluorescens ATCC 17400 consists of treP, treA and treR. The gene treP codes for a putative enzyme II subunit of the phosphotransferase system that catalyzes the phosphorylation of trehalose together with its translocation across the cell membrane and treA encodes a putative phosphot...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)01151-7
更新日期：2000-12-01 00:00:00
abstract：:While the scientific contributions of François Jacob were outstanding, I also consider that his conception of science, and of its place among other forms of knowledge, is also highly original, and important for the future of science in our societies. His contributions to the history and philosophy of science were neit...
journal_title：Research in microbiology
pub_type： 传,历史文章,杂志文章
doi：10.1016/j.resmic.2014.05.001
更新日期：2014-06-01 00:00:00
abstract：:DNA-DNA hybridization showed that the Brucella strains recently isolated from marine mammals belong to the monospecific genus Brucella (more than 77% DNA relatedness). Ribotyping (HindIII rDNA restriction patterns) showed that they may represent a separate subgroup (marine type) specifically associated with marine mam...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)01145-1
更新日期：2000-11-01 00:00:00
abstract：:During an epidemiologic survey, an unidentified strain of Legionella was isolated from water of a thermal spa in France. The strain (Lyon 8420412) had the cultural and biochemical characteristics typical of the genus Legionella. In direct immunofluorescence tests, the strain reacted weakly with fluorescein-conjugated ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90086-7
更新日期：1989-10-01 00:00:00
abstract：:The tolerance of the lactic acid bacterium Oenococcus oeni to hostile wine conditions is essential for the success of malolactic fermentation (MLF). In this study, reverse transcription quantitative PCR (RT-qPCR) was used to quantify the transcript level of 13 genes that could play a role in adaptation of O. oeni in w...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.07.006
更新日期：2006-04-01 00:00:00
abstract：:As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revea...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.11.002
更新日期：2009-03-01 00:00:00
abstract：:The Pseudomonas aeruginosa protein AlgR2 (AlgQ) was originally identified as a regulatory protein implicated in alginate production. It also regulates the synthesis of polyphosphate as well as of a variety of secretable virulence factors, upregulating neuraminidase and siderophore synthesis and downregulating rhamnoli...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(03)00024-X
更新日期：2003-04-01 00:00:00
abstract：:Energy-coupling factor (ECF) transporters mediate the uptake of micronutrients in prokaryotes. They consist of two ATP-binding-cassette family ATPases, a transmembrane coupling protein (T component) and a substrate-binding membrane protein (S component). ECF transporters for Co2+ and Ni2+ ions have one or two addition...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2019.06.007
更新日期：2019-01-01 00:00:00
abstract：:The increase in genetic variability of a population can be selected during adaptation, as demonstrated by the selection of mutator alleles. The dynamics of this phenomenon, named second-order selection, can result in an improved adaptability of bacteria through regulation of all facets of mutation and recombination pr...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(00)01163-3
更新日期：2001-01-01 00:00:00
abstract：:When grown in batch cultures in fermentors with 23.4 mM cellobiose, Clostridium cellulolyticum displayed biphasic growth kinetics not associated with sequential substrate consumption and which led to a twofold higher production of biomass than previously reported. In the first growth phase, acetate was the major produ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)00110-2
更新日期：1999-09-01 00:00:00
abstract：:Legume-nodulating rhizobia use N-acyl homoserine lactones (AHLs) to regulate several physiological traits related to the symbiotic plant-microbe interaction. In this work, we show that Sinorhizobium fredii SMH12, Rhizobium etli ISP42 and Rhizobium sullae IS123, three rhizobial strains with different nodulation ranges,...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.05.002
更新日期：2011-09-01 00:00:00
abstract：:Bacteriophages must destroy the bacterial cell wall to lyse their host and release their progeny into the environment. There are at least two distinct mechanisms by which phages destroy the cell wall. Bacteriophages with large genomes use a holin-endolysin system, while bacteriophages with small genomes encode a singl...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(02)01330-x
更新日期：2002-10-01 00:00:00
abstract：:Brucella spp. are Gram-negative, facultative intracellular bacteria that cause a zoonotic world-wide disease. As in other Gram-negative bacteria, its S-LPS (smooth lipopolysaccharide) is a major determinant of virulence. The Brucella melitensis 16M LPS O-antigen is a homopolymer of 4-formamido-4,6, dideoxymannose. In ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)90130-x
更新日期：2000-10-01 00:00:00
abstract：:Differential immunoproteomics was applied to identify proteins secreted by Metarhizium anisopliae induced by the Rhipicephalus microplus cuticle. In addition, IgG anti-spore surface proteins were used for searching for proteins possibly involved in early stages of fungus versus tick infection. LC-MS/MS of differential...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.09.012
更新日期：2009-12-01 00:00:00
