In-frame fusion of a His-Cys motif into the Pseudomonas aeruginosa outer membrane OprI lipoprotein results in increased metal binding capacity by Escherichia coli.

abstract：:The gene encoding a lipoprotein of 67 kDa, named P67, was cloned from Mycoplasma sp. bovine group 7 strain PG50 and expressed in Escherichia coli K12. Analysis of the amino acid sequence derived from the DNA sequence of the P67 gene revealed a typical prokaryotic signal peptidase II membrane lipoprotein lipid attachme...

journal_title：Research in microbiology

authors： Frey J,Cheng X,Monnerat MP,Abdo EM,Krawinkler M,Bölske G,Nicolet J

更新日期：1998-01-01 00:00:00

abstract：:Bacterial membranes are known to form domains with specific lipid compositions and functions. Recently, using membrane binding fluorescent dyes, lipid spiral structures extending along the long axis of the cell were detected. These spirals were absent when the synthesis of phosphatidylglycerol and cardiolipin was disr...

journal_title：Research in microbiology

authors： Muchová K,Jamroškovič J,Barák I

更新日期：2010-11-01 00:00:00

abstract：:Several cDNA fragments corresponding to transcripts differentially expressed under conditions that favor mycelial growth of Candida albicans were identified by the "differential display" technique. One of these was cloned and used as a probe to rescue the full gene from a genomic library of the fungus. The sequence id...

journal_title：Research in microbiology

authors： Orozco I,Ortiz L,Elorza MV,Ruiz-Herrera J,Sentandreu R

更新日期：2002-11-01 00:00:00

abstract：:Monoclonal antibody II-6-18 recognizes a serogroup-1-specific Legionella pneumophila antigenic determinant which has been shown to be virulence-associated. We previously reported the physicochemical characterization by means of a quantitative fluorometric assay of monoclonal antibody II-6-18 binding to L. pneumophila,...

journal_title：Research in microbiology

authors： Petitjean F,Dournon E,Strosberg AD,Hoebeke J

更新日期：1990-11-01 00:00:00

abstract：:Microcin E492 (MccE492) is an antibacterial protein whose activity on target cells requires ManYZ, the inner membrane component of the mannose permease. We show here that MceA, the polypeptide core of MccE492, stably associates with ManYZ both in the presence and in the absence of MceB, the MccE492 immunity protein. T...

journal_title：Research in microbiology

authors： Biéler S,Silva F,Belin D

更新日期：2010-10-01 00:00:00

abstract：:The psychotrophic strain Pseudomonas fluorescens MFO is known to express several enzymatic activities in milk, including extracellular proteolytic activity, optimally when cells are grown at 17.5 degrees C. In order to study the nature of the mechanisms controlling the production of the extracellular protease, we devi...

journal_title：Research in microbiology

authors： Hellio FC,Orange N,Guespin-Michel JF

更新日期：1993-10-01 00:00:00

abstract：:Bacillus laterosporus is a spore-forming bacterium characterized by its ability to produce a canoe-shaped lamellar parasporal inclusion, adjacent to the spore. In some B. laterosporus strains crystalline inclusions of various shapes and sizes, which are released separately from spores during the lysis of the sporangiu...

journal_title：Research in microbiology

authors： Smirnova TA,Minenkova IB,Orlova MV,Lecadet MM,Azizbekyan RR

更新日期：1996-06-01 00:00:00

abstract：:In contrast to bacteria, mitochondria contain only a few ATP binding cassette (ABC) transporters in their inner membrane. The known mitochondrial ABC proteins fall into two major classes that, in the yeast Saccharomyces cerevisiae, are represented by the half-transporter Atm1p and the two closely homologous proteins M...

journal_title：Research in microbiology

authors： Lill R,Kispal G

更新日期：2001-04-01 00:00:00

abstract：:The removal of water through air drying damages membranes, proteins and nucleic acids and is lethal to the majority of organisms. Nevertheless, some vegetative cells of bacteria and cyanobacteria survive extreme desiccation. Understanding the mechanisms of their desiccation tolerance is an important issue in cell biol...

journal_title：Research in microbiology

authors： Billi D,Potts M

更新日期：2002-01-01 00:00:00

abstract：:Spirochetes have a unique motility system that is characterized by flagellar filaments contained within the outer membrane sheath. Direct evidence using video microscopy has recently been obtained which indicates that these periplasmic flagella (PF) rotate in several spirochetal species. This rotation generates thrust...

journal_title：Research in microbiology

authors： Charon NW,Greenberg EP,Koopman MB,Limberger RJ

更新日期：1992-07-01 00:00:00

abstract：:Paralytic shellfish toxins (PSTs) are sodium channel blocking (SCB) toxins, produced by cyanobacteria, as well as by marine dinoflagellates and their associated bacteria, and cause serious health and economic concern worldwide. In a previous study, approximately 70% of the bacteria enriched from PST-contaminated shell...

journal_title：Research in microbiology

authors： Vásquez M,Grüttner C,Möeller B,Moore ER

更新日期：2002-07-01 00:00:00

abstract：:Most recently published complete bacterial genomes have revealed unexpectedly high numbers of long strict repeats. In this article we discuss the various functional and evolutionary roles of these repeats, focusing in particular on their role in terms of genome stability, gene transfer, and antigenic variation. ...

journal_title：Research in microbiology

authors： Rocha EP,Danchin A,Viari A

更新日期：1999-11-01 00:00:00

abstract：:The Escherichia coli mannitol permease (EIIMtl) of the phosphoenolpyruvate-dependent phosphotransferase system is a 68-kDa membrane protein that carries out the concomitant transport and phosphorylation of D-mannitol. Previous studies indicated that there are ca. 6 membrane-spanning helices within the N-terminal half ...

journal_title：Research in microbiology

authors： Briggs CE,Khandekar SS,Jacobson GR

更新日期：1992-02-01 00:00:00

abstract：:Mercury methylation and demethylation processes govern the fate of methylmercury in aquatic ecosystems. Under anoxic conditions, methylation activity is mainly of biological origin and is often the result of sulfate-reducing bacteria. In this study, the use of a luminescent biosensor for screening methylmercury produc...

journal_title：Research in microbiology

authors： Colin Y,Gury J,Monperrus M,Gentes S,Ayala Borda P,Goni-Urriza M,Guyoneaud R

更新日期：2018-01-01 00:00:00

abstract：:Bacillus cereus is widely distributed in the environment and is responsible for foodborne illnesses, causing diarrhea and vomiting. In this study, the prevalence of B. cereus bacteriophages in Korean fermented foods was studied using 6 reference strains, and JBP901 was further characterized. In a total of 47 samples, ...

journal_title：Research in microbiology

authors： Shin H,Bandara N,Shin E,Ryu S,Kim KP

更新日期：2011-10-01 00:00:00

abstract：:The major 25-kDa outer membrane protein (Omp25) of Brucella abortus was purified and antigenically characterized by use of monoclonal antibodies (mAbs). Purification was achieved from the sodium dodecyl sulphate-insoluble (SDS-I) cell wall (CW) fraction of vaccine strain B. abortus B19 which was shown by use of mAbs t...

journal_title：Research in microbiology

authors： Cloeckaert A,Zygmunt MS,Bézard G,Dubray G

更新日期：1996-05-01 00:00:00

abstract：:We have used a mouse Peyer's patch gut loop model to investigate the role of the intestinal membranous epithelial (M) cells in the pathogenesis of Salmonella typhimurium. These specialized antigen sampling cells are located in the follicle-associated epithelium (FAE) overlying the isolated and aggregated lymphoid foll...

journal_title：Research in microbiology

authors： Clark MA,Jepson MA,Simmons NL,Hirst BH

更新日期：1994-09-01 00:00:00

abstract：:Several specific primers for the nifH gene were tested with different pure telluric N2-fixing strains. A PolF/PolR primer set provided successful amplification of 19 representative N2-fixing strains. Three restriction enzymes, HaeIII, NdeII and MnlI, chosen for restriction fragment length polymorphism (RFLP) analyses,...

journal_title：Research in microbiology

authors： Poly F,Monrozier LJ,Bally R

更新日期：2001-01-01 00:00:00

abstract：:Translation termination in eukaryotes is mediated by two polypeptide chain-release factors, eRF1 and eRF3. eRF1 recognizes stop signals, while eRF3 is a ribosome-dependent and eRF1-dependent GTPase. eRF1 forms a stable complex with eRF3 in vivo and in vitro. In the present study, a variety of truncated forms of Euplot...

journal_title：Research in microbiology

authors： Song L,Chai BF,Wang W,Liang AH

更新日期：2006-11-01 00:00:00

abstract：:To understand antibiotic resistance in Gram-negative bacteria, a key point is to investigate antibiotic accumulation, which is defined by influx and efflux. Several methods exist to evaluate membrane permeability and efflux pump activity, but they present disadvantages and limitations. An optimized spectrofluorimetric...

journal_title：Research in microbiology

authors： Masi M,Dumont E,Vergalli J,Pajovic J,Réfrégiers M,Pagès JM

更新日期：2018-01-01 00:00:00

abstract：:Rhizobium leguminosarum bv. trifolii synthesizes extracellular polysaccharide (EPS) that is postulated to be a biologically active signalling molecule in clover symbiosis. A group of seven exopolysaccharide-deficient (Exo), non-nitrogen-fixing mutants of R. leguminosarum bv. trifolii strain 24.1 isolated by transposon...

journal_title：Research in microbiology

authors： Janczarek M,Skorupska A

更新日期：2003-07-01 00:00:00

abstract：:Both Neisseria meningitidis and Streptococcus pneumoniae are naturally transformable species and are known to be freely recombining in the wild. Large multilocus sequence typing (MLST) datasets have been generated for these species. Here we outline an approach which exploits these data sets in order to quantify the ex...

journal_title：Research in microbiology

authors： Feil EJ,Enright MC,Spratt BG

更新日期：2000-07-01 00:00:00

abstract：:The structure of DspF, a type III secretion system (T3SS) chaperone required for virulence of the fruit tree pathogen Erwinia amylovora, was modeled based on predicted structural homology to characterized T3SS chaperones. This model guided the selection of 11 amino acid residues that were individually mutated to alani...

journal_title：Research in microbiology

authors： Triplett LR,Wedemeyer WJ,Sundin GW

更新日期：2010-09-01 00:00:00

abstract：:Some of the present problems in ribotyping are associated with a lack of uniform reactivity of probes when bacterial DNAs are of phylogenetically diverse origins. To overcome these problems, a set of five oligonucleotides (referred to as OligoMix5) was selected to react with conserved sequences located near both extre...

journal_title：Research in microbiology

authors： Regnault B,Grimont F,Grimont PA

更新日期：1997-11-01 00:00:00

abstract：:A strain of Bacteroides fragilis, isolated from the marmoset Callithrix penicillata, produced protein(s) with bacteriocin activity (fragicilin). Two active fractions (36 and 150 kDa) were isolated by chromatography. The bacteriocin exhibited iso- and heteroantagonism. It remained stable between pH 3 and 10 and at 60 d...

journal_title：Research in microbiology

authors： Farias LM,Totola AH,Miranda CM,Carvalho MA,Damasceno CA,Tavares CA,Cisalpino EO,Vieira EC

更新日期：1994-01-01 00:00:00

abstract：:A new type of nitrogen-fixing, oxalate-oxidizing Azospirillum sp. was isolated from the roots of Oxalis pes-caprae. Polyphasic taxonomy was performed, including auxanography using API galleries, physiological tests and 16S rRNA sequence comparison. Optimum growth occurred at 30 degrees C, pH 7.5. Growth was observed a...

journal_title：Research in microbiology

authors： Sahin N

更新日期：2005-04-01 00:00:00

abstract：:FliA is known to be a sigma factor that regulates bacterial flagella gene expression. Accumulating evidence suggests that FliA is involved in bacterial behavior other than motility. To elucidate the contribution of FliA to Pseudomonas aeruginosa pathophysiology, we analyzed the biological properties and gene expressio...

journal_title：Research in microbiology

authors： Lo YL,Chen CL,Shen L,Chen YC,Wang YH,Lee CC,Wang LC,Chuang CH,Janapatla RP,Chiu CH,Chang HY

更新日期：2018-04-01 00:00:00

abstract：:A method which enables selection of protein mutants impaired in their ability to interact with their normal protein partners is presented here. The method is the two-phage two-hybrid assay adapted for mutant selection. In the two-phage assay, the interaction between two proteins enables the formation of a functional h...

journal_title：Research in microbiology

authors： Barbati S,Grenga L,Luzi G,Paolozzi L,Ghelardini P

更新日期：2010-03-01 00:00:00

abstract：:Urease biogenesis was monitored in the lactic acid bacterium Streptococcus thermophilus during the growth cycle using in-gel detection and a phenol-hypochloride assay. Zymogram analysis, performed in a non-denaturing polyacrylamide gel, enabled visualization of a complex profile of bands whose number and intensity wer...

journal_title：Research in microbiology

authors： Mora D,Monnet C,Parini C,Guglielmetti S,Mariani A,Pintus P,Molinari F,Daffonchio D,Manachini PL

更新日期：2005-11-01 00:00:00

abstract：:This study evaluated two generic polymerase chain reaction (PCR) protocols, and nine subtyping protocols and three PCR-restriction fragment length polymorphism (RFLP) protocols for detection of stx genes. The PCR protocols were evaluated by testing 12 reference isolates and 496 field strains of Shiga toxin-producing E...

journal_title：Research in microbiology

authors： Ziebell KA,Read SC,Johnson RP,Gyles CL

更新日期：2002-06-01 00:00:00