Abstract:
:Translation termination in eukaryotes is mediated by two polypeptide chain-release factors, eRF1 and eRF3. eRF1 recognizes stop signals, while eRF3 is a ribosome-dependent and eRF1-dependent GTPase. eRF1 forms a stable complex with eRF3 in vivo and in vitro. In the present study, a variety of truncated forms of Euplotes octocarinatus eRF3 were created, and systematic analysis of the interaction between E. octocarinatus eRF1a and these eRF3 mutants was performed by employing both in vivo a yeast two-hybrid assay and in vitro a pull-down assay. The results demonstrated that a short portion of the C-terminal domain of eRF3 is sufficient for eRF1a binding in E. octocarinatus. Specifically, the eRF1a-binding sites on eRF3 are located at a region containing amino acid residues 640-723 in E. octocarinatus eRF3. Amino acid sequence analysis of eRF3 from E. octocarinatus, humans and yeast showed that the eRF1a binding domain on E. octocarinatus eRF3 was similar to that of yeast eRF3 but different from that of human eRF3.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Song L,Chai BF,Wang W,Liang AHdoi
10.1016/j.resmic.2006.07.005subject
Has Abstractpub_date
2006-11-01 00:00:00pages
842-50issue
9eissn
0923-2508issn
1769-7123pii
S0923-2508(06)00153-7journal_volume
157pub_type
杂志文章abstract::Twenty-one Branhamella catarrhalis strains selected for their diversity by esterase electrophoretic polymorphism, and belonging to 20 distinct zymotypes, were studied by restriction fragment length polymorphism (RFLP) of total DNA after ethidium bromide staining and of ribosomal DNA regions (ribotyping). The former an...
journal_title:Research in microbiology
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journal_title:Research in microbiology
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abstract::By employing flow cytometry, the DNA content and cell size of individual bacterial cells may be determined rapidly and with high precision. Also, the number of DNA replication origins in Escherichia coli cells can be measured after treating the cells with rifampicin together with the cell division inhibitor cephalexin...
journal_title:Research in microbiology
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doi:10.1016/s0923-2508(00)00222-9
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pub_type: 杂志文章
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更新日期:2010-03-01 00:00:00
abstract::A highly effective chlorpyrifos-degrading bacterium strain Dsp-2 was isolated from the polluted treatment system of a chlorpyrifos manufacturer. This strain was preliminarily identified as Sphingomonas sp. based on its morphological, physiological and biochemical tests as well as 16S rDNA analysis. It utilized chlorpy...
journal_title:Research in microbiology
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更新日期:2007-03-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
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更新日期:1995-09-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90031-5
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.05.008
更新日期:2004-11-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(98)80304-5
更新日期:1998-04-01 00:00:00
abstract::A collection of 57 strains of hyperthermophilic Archaea from the order Thermococcales was screened for the presence of plasmids; 9 plasmids present in six of these strains were isolated and characterized in terms of size and cross-hybridization. The Notl macrorestriction patterns of genomic DNA of strains harbouring t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(97)82452-7
更新日期:1997-12-01 00:00:00
abstract::The mechanism by which TrwB acts as a DNA transporter in bacterial conjugation is analyzed. Based on a parallelism between TrwB and F(1)-ATPase, TrwB would use the energy derived from ATP hydrolysis to pump DNA through its central channel, in a manner similar to that used by F(1)-ATPase to produce a rotary movement of...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2005.12.002
更新日期:2006-05-01 00:00:00
abstract::Sub-MIC antibiotics differentially modulate transcription of subsets of genes by unknown mechanisms. Paradoxically, the RNA polymerase inhibitor rifampicin is able to both upmodulate as well as downmodulate transcription when present at sub-MIC levels. In this study, we analyzed DNA sequences required for transcriptio...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.02.003
更新日期:2013-06-01 00:00:00
abstract::Magnetotactic bacteria (MTB) are a group of Gram-negative bacteria synthesizing magnetic crystals that allow them aligning along magnetic field lines. They have diverse morphologies including cocci, rods, vibrio, spirilla, and multicellular magnetotactic prokaryotes (MMPs). MMPs are composed of 10-57 cells with peritr...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2014.07.012
更新日期:2014-09-01 00:00:00
abstract::A total of 28 species of Legionella could be differentiated by rRNA gene restriction patterns generated after cleavage of total DNA with either EcoRV or HindIII restriction endonucleases, and hybridization of fragments with 32P-labelled Escherichia coli 16 + 23S rRNA. Different species gave different fragment patterns...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90193-9
更新日期:1989-11-01 00:00:00
abstract::A novel acidophilic member of the phylum Actinobacteria was isolated from an acidic stream draining an abandoned copper mine in north Wales. The isolate (PY-F3) was demonstrated to be a heterotroph that catalyzed the oxidation of ferrous iron (but not of sulfur or hydrogen) under aerobic conditions, and the reduction ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2015.01.003
更新日期:2015-02-01 00:00:00
abstract::In contrast to bacteria, mitochondria contain only a few ATP binding cassette (ABC) transporters in their inner membrane. The known mitochondrial ABC proteins fall into two major classes that, in the yeast Saccharomyces cerevisiae, are represented by the half-transporter Atm1p and the two closely homologous proteins M...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(01)01204-9
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abstract::Chinoike Jigoku ("Blood Pond Hell") is located in the hot spring town of Beppu on the southern island of Kyushu in Japan, and is the site of a red-colored acidic geothermal pond. This study aimed to investigate the microbial population composition in this extremely acidic environment and to isolate/characterize acidop...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.04.011
更新日期:2016-09-01 00:00:00
abstract::For nearly one hundred years, researchers have attempted to categorize botulinum neurotoxin-producing clostridia and the toxins that they produce according to biochemical characterizations, serological comparisons, and genetic analyses. Throughout this period the bacteria and their toxins have defied such attempts at ...
journal_title:Research in microbiology
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.resmic.2014.09.007
更新日期:2015-05-01 00:00:00
abstract::In this study, the use of flow cytometry to analyze phage-mediated killing of Enterobacter aerogenes under varying conditions of temperature and nutrient availability was assessed. Bacteriophage UZ1, specific for an E. aerogenes strain, was applied at a multiplicity of infection (MOI) of 1 and 1000 to a Teflon surface...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.02.007
更新日期:2006-09-01 00:00:00
abstract::Most recently published complete bacterial genomes have revealed unexpectedly high numbers of long strict repeats. In this article we discuss the various functional and evolutionary roles of these repeats, focusing in particular on their role in terms of genome stability, gene transfer, and antigenic variation. ...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(99)00120-5
更新日期:1999-11-01 00:00:00
abstract::The sensitivity and specificity of 7 PCR assays described for the identification of Campylobacter jejuni and Campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of C. jejuni, C. coli, Campylobacter lari and related Campylobacter, Helicobacter and A...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2007.11.020
更新日期:2008-03-01 00:00:00
abstract::In this study, we identified an antimicrobial compound produced by the Gram-negative bacterium Serratia marcescens. Colonies of S. marcescens inhibited the growth of nine different methicillin-resistant Staphylococcus aureus (MRSA) isolates and several other tested Gram-positive bacterial species, but not Gram-negativ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.06.002
更新日期:2013-10-01 00:00:00
abstract::rRNA gene restriction patterns (ribotyping) were compared with phage typing, serotyping, enterotoxins and exfoliatin production in the analysis of 26 Staphylococcus aureus strains isolated from two different nosocomial outbreaks. Total DNA was cleaved by EcoRI restriction endonuclease. After agarose gel electrophoresi...
journal_title:Research in microbiology
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(98)80078-8
更新日期:1998-03-01 00:00:00
abstract::From the nose of a patient with aspiration pneumonia, we isolated a new bacterium by amoebal co-culture using Acanthamoeba polyphaga. Based on phenotypic, 16S rRNA and phylogenetic analysis, we show that this isolate represents a new species within the genus Rhodobacter and and we have named it Rhodobacter massiliensi...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2003.08.002
更新日期:2003-11-01 00:00:00
abstract::The adsorption complex of filamentous phage fd consists of two minor coat proteins, g3p and g6p, and is considered to be not only a structural entity, but also a functional unit to terminate phage assembly. Cells were infected with phage M13am8H1, which cannot assemble because it lacks the major coat protein g8p, alth...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(94)90042-6
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