Abstract：
:A highly effective chlorpyrifos-degrading bacterium strain Dsp-2 was isolated from the polluted treatment system of a chlorpyrifos manufacturer. This strain was preliminarily identified as Sphingomonas sp. based on its morphological, physiological and biochemical tests as well as 16S rDNA analysis. It utilized chlorpyrifos as its sole source of carbon for growth, by hydrolyzing chlorpyrifos to 3,5,6-trichloro-2-pyridinol (TCP). It could also utilize parathion, parathion-methyl, fenitrothion and profenofos, but not phoxin and triazophos. Bioremediation of chlorpyrifos-contaminated soil was examined using Dsp-2. Dsp-2 addition to soil treated with 100mgkg(-1) chlorpyrifos resulted in a higher degradation rate than control soils without inoculation. The moderate pH, moisture and inoculum density could have promoted degradation. The gene encoding the chlorpyrifos hydrolytic enzyme was cloned by PCR. Although BLAST sequence search results indicated that this gene has 99% similarity to mpd (a gene encoding the parathion-methyl hydrolyzing enzyme in Plesiomonas sp. M6), its hydrolytic efficiency for chlorpyrifos was significantly greater than the wild-type mpd from strain M6.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Li X,He J,Li Sdoi
10.1016/j.resmic.2006.11.007subject
Has Abstractpub_date
2007-03-01 00:00:00pages
143-9issue
2eissn
0923-2508issn
1769-7123pii
S0923-2508(06)00253-1journal_volume
158pub_type
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