Abstract：
:The promoter probe mini-Tn5-luxAB-tet was used to create a luxAB transcriptional fusion responding to fluorene in the fluorene utilising bacterium Sphingomonas sp. LB126. The mutant strain, named L-132, was impaired in fluorene utilisation and strongly emitted light upon addition of fluorene to the growth medium. L-132 was initially characterised and examined for its potential use as a whole-cell biosensor in the perspective of quantifying fluorene in environmental samples. Activity of the reporter gene as a response to fluorene was detectable after 30 min and was optimal after 4 h. A linear response to fluorene concentrations within the water solubility range was achieved, with a detection limit of 200 microg per litre. Besides fluorene, L-132 weakly responded to the polycyclic aromatic hydrocarbons phenanthrene and dibenzothiophene, whereas strong responses were obtained with 9-fluorenone, 9-hydroxyfluorene, phthalic acid and protocatechuic acid. The latter four compounds are metabolites formed in course of fluorene degradation, which suggested that a fluorene metabolite rather than fluorene itself was the true inducer of the luxAB fusion in L-132.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Bastiaens L,Springael D,Dejonghe W,Wattiau P,Verachtert H,Diels Ldoi
10.1016/s0923-2508(01)01268-2subject
Has Abstractpub_date
2001-12-01 00:00:00pages
849-59issue
10eissn
0923-2508issn
1769-7123pii
S0923-2508(01)01268-2journal_volume
152pub_type
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