Abstract：
:Adhesion to the intestinal mucosa is considered to be one of the main selection criteria of lactic acid bacteria for probiotic use. Adhesive probiotics are, for example, considered to provide better antagonism against pathogenic bacteria when compared to non-adhesive strains. Here a new model is described for studying adhesion and interaction of probiotic and pathogenic bacteria in the intestinal mucus in which the intestinal microbiota is present. The model is based on the use of human intestinal tissue, fluorescent-tagged bacteria and confocal laser scanning microscopy (CLSM) in adhesion measurements as well as human intestinal mucus and bioluminescent-tagged bacteria in viability measurements. Use of CLSM enabled, for the first time, real-time three-dimensional observations of live probiotic bacteria in their natural environment, the intestinal mucosa. When the real-time measurement of bacterial adhesion was combined with the real-time sensitive measurement of bacterial viability, it could be studied whether or not the adherent pathogens were alive. The model was used to study the interaction between Lactobacillus rhamnosus GG and Salmonella enterica serovar Typhimurium. We show that L. rhamnosus GG did not affect the adhesion or the viability of S. enterica serovar Typhimurium. Instead S. enterica serovar Typhimurium was shown to decrease the adhesion of L. rhamnosus GG in displacement assays. Moreover, the method is suitable for studies in which the interaction of two or more bacteria is examined in an environment in which other bacteria are present.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Vesterlund S,Paltta J,Karp M,Ouwehand ACdoi
10.1016/j.resmic.2004.08.012subject
Has Abstractpub_date
2005-03-01 00:00:00pages
238-44issue
2eissn
0923-2508issn
1769-7123pii
S0923-2508(04)00215-3journal_volume
156pub_type
杂志文章abstract：:Ferric reductase catalyzes the reduction of ferric iron into ferrous iron and plays an essential role in high-affinity iron acquisition. In this study, we found that the cfl1Δ/Δ (orf19.1263) mutant was not defective in iron acquisition. However, deletion of CFL1 increased cellular iron accumulation by elevating surfac...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.03.001
更新日期：2014-04-01 00:00:00
abstract：:At the beginning of the 20th century, it was discovered at the Pasteur Institute in Tunis that epidemic typhus is transmitted by the human body louse. The complete genome sequence of its causative agent, Rickettsia prowazekii, was determined at Uppsala University in Sweden at the end of the century. In this mini-revie...
journal_title：Research in microbiology
pub_type： 传,历史文章,杂志文章,评审
doi：10.1016/s0923-2508(00)00116-9
更新日期：2000-03-01 00:00:00
abstract：:During the past twenty-nine years, not a single class of antimicrobial agents has been discovered that has led to new approved human drugs. Despite a dramatic increase in the potency of existing classes, the need for new effective antimicrobial agents continues. The bacterial phosphotransferase system (PTS) offers the...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(92)90089-7
更新日期：1992-06-01 00:00:00
abstract：:The present mini-review summarizes data in selected fields of basic genetics which were exclusively obtained in agreement with the principles of SOS Chromotest methodology and with Escherichia coli PQ37 sfiA::lacZ as a tester strain. ...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(02)01342-6
更新日期：2002-09-01 00:00:00
abstract：:The atmospheric concentration of methane (CH(4)), a major greenhouse gas, is mainly controlled by the activities of methane-producing (methanogens) and methane-consuming (methanotrophs) microorganisms. Freshwater lakes are identified as one of the main CH(4) sources, as it was estimated that they contribute to 6-16% o...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.06.004
更新日期：2011-11-01 00:00:00
abstract：:Although it seems obvious that fever has some important general adaptive value, it is still not clear by what means this function is manifested. One postulate is that febrile conditions result in the sequestration of soluble iron, effectively starving some pathogens of that essential nutrient. On the basis of our rece...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(94)90182-1
更新日期：1994-05-01 00:00:00
abstract：:Lectin-like substances of 54 marine algae, corresponding to 30 red algae and 24 brown algae, were studied to estimate the agglutination activity against Candida guilliermondii. Extracts of Dictyopteris membranacea, Bifurcaria bifurcata, Halidrys siliquosa, Ascophyllum nodosum, Fucus serratus and F. spiralis algae show...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90013-2
更新日期：1989-07-01 00:00:00
abstract：:Sequences of the lacSZ operon of 29 Streptococcus thermophilus strains from different dairy products were determined. Differences in sequence among the strains were detected within LacS more often than in the LacZ gene. The sequences were aligned and compared and it was possible to gather the strains into three groups...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.09.005
更新日期：2005-03-01 00:00:00
abstract：:The rRNA gene restriction patterns of 89 Vibrio cholerae O1 isolates from different geographic origins were studied. The probe was Escherichia coli 16 + 23S rRNA labelled with "ECL Gene detection system". A total of 17 rRNA gene restriction patterns were observed after BglI cleavage. Four patterns (B1 to B4) were only...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(90)90059-y
更新日期：1990-07-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.07.005
更新日期：2013-11-01 00:00:00
abstract：:The possibility of using kaolinite-immobilized plasmodium fragments of Physarella oblonga (Berk. & Curt.) Morgan to maintain their metabolic activity was examined. The immobilization process was carried out with 1 mg of plasmodium of P. oblonga entrapped in 10 g of kaolinite. Sodium acetate (1 mM) was used as a metabo...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(02)00008-6
更新日期：2003-01-01 00:00:00
abstract：:Randomly cloned fragments (in a size range 1 to 2.5 kb) of DNA from Bifidobacterium longum ATCC 15707, B. adolescentis CIP 64.59T, B. bifidum CIP 64.65 and B. animalis ATCC 25527 were used as hybridization probes to characterize strains of these species and distinguish them from closely related Bifidobacterium species...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)80271-3
更新日期：1995-01-01 00:00:00
abstract：:Long polar fimbriae (LPF) are related to type I fimbriae in genetic organization and were first identified in Salmonella enterica serovar Typhimurium. Four lpfA genetic variants designated lpfA(O157/OI-141), lpfA(O157/OI-154), lpfA(O26) and lpfA(O113) have been identified in Shiga toxin-producing Escherichia coli (STE...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.06.009
更新日期：2006-03-01 00:00:00
abstract：:Bacillus cereus is widely distributed in the environment and is responsible for foodborne illnesses, causing diarrhea and vomiting. In this study, the prevalence of B. cereus bacteriophages in Korean fermented foods was studied using 6 reference strains, and JBP901 was further characterized. In a total of 47 samples, ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.07.001
更新日期：2011-10-01 00:00:00
abstract：:Ecologists have documented the process of plant succession for centuries, yet the successional patterns exhibited by microbial communities have received relatively little attention. We examine recent work on microbial succession and show how, despite some key differences, studies of plant succession can serve as a tem...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2010.06.002
更新日期：2010-10-01 00:00:00
abstract：:Mollicute evolution has been marked by significant changes in genome structure and use of their genetic information. These include a reduction in their genome G+C content and the use by most mollicutes of the UGA universal stop codon as tryptophan. More striking is the size reduction in their genome which, for some sp...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.09.007
更新日期：2007-12-01 00:00:00
abstract：:Bacillus species as a group offer several advantages over other bacteria for protection against root pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. The objectives of this work were to determine the ability of strains of Bacillus to inhibit Fusari...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.03.001
更新日期：2005-06-01 00:00:00
abstract：:The sensitivity and specificity of 7 PCR assays described for the identification of Campylobacter jejuni and Campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of C. jejuni, C. coli, Campylobacter lari and related Campylobacter, Helicobacter and A...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.11.020
更新日期：2008-03-01 00:00:00
abstract：:(51)V NMR spectroscopy was used for detection and identification of cell-associated vanadate (V(5+)) species after exposure of Phycomyces blakesleeanus mycelium, in exponential phase of growth, to sodium orthovanadate. Complete disappearance of monomer and dimer signals and decreased intensity of the tetramer signal w...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.04.012
更新日期：2016-07-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(03)00113-X
更新日期：2003-07-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2017.03.009
更新日期：2017-01-01 00:00:00
abstract：:Vegetative incompatibility limits heterokaryon formation in fungi. It results from genetic differences at specific loci (het loci). Characterization of het genes and, more recently, of incompatibility reaction suppressors has, provided insight into the mechanisms involved. A link between development, vegetative incomp...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(00)00145-5
更新日期：2000-05-01 00:00:00
abstract：:Three primers derived from the lap gene were used to distinguish Listeria monocytogenes from L.innocua and other Listeria species. L. monocytogenes and L. innocua yielded a PCR product of 600 and 300 bp, respectively, whereas a typical pattern of three amplimers was observed with L. ivanovii, L. seeligeri and L. welsh...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)84020-4
更新日期：1996-10-01 00:00:00
abstract：:The major 25-kDa outer membrane protein (Omp25) of Brucella abortus was purified and antigenically characterized by use of monoclonal antibodies (mAbs). Purification was achieved from the sodium dodecyl sulphate-insoluble (SDS-I) cell wall (CW) fraction of vaccine strain B. abortus B19 which was shown by use of mAbs t...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)81383-0
更新日期：1996-05-01 00:00:00
abstract：:Several cDNA fragments corresponding to transcripts differentially expressed under conditions that favor mycelial growth of Candida albicans were identified by the "differential display" technique. One of these was cloned and used as a probe to rescue the full gene from a genomic library of the fungus. The sequence id...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(02)01373-6
更新日期：2002-11-01 00:00:00
abstract：:In order to simplify diagnostic susceptibility testing of mycobacteria against thiacetazone, hydroxylamine, p-nitrobenzoic acid and picric acid, we modified the standard procedure by using Middlebrook 7H11 agar in a single quadrant petri dish. This plate can be inoculated and read in parallel with routine susceptibili...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90078-8
更新日期：1989-03-01 00:00:00
abstract：:Growth of fluoroquinolone (FQ)-susceptible methicillin-resistant Staphylococcus aureus (MRSA) in the presence of sub-MIC FQ has been shown to enhance methicillin resistance in traditional nosocomial MRSA isolates. We aimed to confirm this phenomenon in nine community-associated MRSA (CA-MRSA) clinical isolates, and to...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.03.003
更新日期：2009-04-01 00:00:00
abstract：:The psychotrophic strain Pseudomonas fluorescens MFO is known to express several enzymatic activities in milk, including extracellular proteolytic activity, optimally when cells are grown at 17.5 degrees C. In order to study the nature of the mechanisms controlling the production of the extracellular protease, we devi...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(93)90064-9
更新日期：1993-10-01 00:00:00
abstract：:A method which enables selection of protein mutants impaired in their ability to interact with their normal protein partners is presented here. The method is the two-phage two-hybrid assay adapted for mutant selection. In the two-phage assay, the interaction between two proteins enables the formation of a functional h...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2010.01.001
更新日期：2010-03-01 00:00:00
abstract：:Clostridium tyrobutyricum is an anaerobic bacterium responsible for late blowing defects during cheese ripening and it is of scientific interest for biological hydrogen production. A scanning electron microscopy (SEM) coating technique and X-ray microanalysis were developed to analyze the architecture and chemical com...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.04.003
更新日期：2009-06-01 00:00:00
