Abstract:
:Paralytic shellfish toxins (PSTs) are sodium channel blocking (SCB) toxins, produced by cyanobacteria, as well as by marine dinoflagellates and their associated bacteria, and cause serious health and economic concern worldwide. In a previous study, approximately 70% of the bacteria enriched from PST-contaminated shellfish tissue and isolated on marine agar medium were observed to produce SCB toxins. In the study reported here, the high percentage of cultivable toxigenic bacteria is demonstrated to be obtained through a marked selection on marine agar medium. The cultivable as well as the total bacterial diversity associated with PST-contaminated shellfish collected from the Magallanes region in the south of Chile has been analysed. Approximately 80% of bacterial isolates, analysed by restriction analysis of PCR amplified ribosomal DNA (i.e., ARDRA fingerprinting), were limited to only two genotypic OTUs (operational taxonomic unit). Sequence determination and analysis of the 16S rDNA from representative isolates of both OTUs established them to be closely related to species of the Psychrobacter genus of the gamma-subclass of the Proteobacteria. The total bacterial diversity in the shellfish was further analysed, using a cultivation-independent strategy of extraction of total DNA from contaminated tissue, PCR-amplification of bacterial 16S rRNA genes, cloning of the PCR products and analysis of the cloned 16S rDNA sequence types by fingerprinting and sequencing. Only 2% of the cloned sequence types corresponded to species of the Psychrobacter genus. The 16S rDNA sequence types detected clustered with species of the y-Proteobacteria subclass, the Cytophaga-Flexibacter-Bacteroides (CFB), the Fusobacteria and the Firmicutes phyla. The level of diversity observed within the libraries of cloned 16S rDNA was markedly greater than that observed among isolates obtained through marine agar enrichment cultures from the same shellfish tissue. Additionally the predominant cloned 16S rDNA sequence types detected from samples of the surrounding seawater demonstrated no correlation with those observed in the PST-contaminated mussels.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Vásquez M,Grüttner C,Möeller B,Moore ERdoi
10.1016/s0923-2508(02)01328-1subject
Has Abstractpub_date
2002-07-01 00:00:00pages
333-8issue
6eissn
0923-2508issn
1769-7123pii
S0923-2508(02)01328-1journal_volume
153pub_type
杂志文章abstract::Association with a surface in a structure known as biofilm is the prevailing microbial lifestyle. Here we show the kinetics of biofilm formation of Escherichia coli W3110 in static cultures growing under aerobic or anaerobic conditions. Aerobically growing cells in LB medium started to produce detectable amounts of bi...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.03.004
更新日期:2004-09-01 00:00:00
abstract::The aim of the present work was to develop polymerase chain reactions (PCRs) based on the conserved nucleotide sequence of the 16S rRNA gene for detection of bacteria of the Helicobacter genus in human antral biopsy samples. The assay for Helicobacter spp was developed by amplifying a 399-bp 16S rRNA gene sequence spe...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/S0923-2508(97)81587-2
更新日期:1997-05-01 00:00:00
abstract::During the past twenty-nine years, not a single class of antimicrobial agents has been discovered that has led to new approved human drugs. Despite a dramatic increase in the potency of existing classes, the need for new effective antimicrobial agents continues. The bacterial phosphotransferase system (PTS) offers the...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/0923-2508(92)90089-7
更新日期:1992-06-01 00:00:00
abstract::FliA is known to be a sigma factor that regulates bacterial flagella gene expression. Accumulating evidence suggests that FliA is involved in bacterial behavior other than motility. To elucidate the contribution of FliA to Pseudomonas aeruginosa pathophysiology, we analyzed the biological properties and gene expressio...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2018.02.001
更新日期:2018-04-01 00:00:00
abstract::Bacteriophages must destroy the bacterial cell wall to lyse their host and release their progeny into the environment. There are at least two distinct mechanisms by which phages destroy the cell wall. Bacteriophages with large genomes use a holin-endolysin system, while bacteriophages with small genomes encode a singl...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(02)01330-x
更新日期:2002-10-01 00:00:00
abstract::We have used a mouse Peyer's patch gut loop model to investigate the role of the intestinal membranous epithelial (M) cells in the pathogenesis of Salmonella typhimurium. These specialized antigen sampling cells are located in the follicle-associated epithelium (FAE) overlying the isolated and aggregated lymphoid foll...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(94)90031-0
更新日期:1994-09-01 00:00:00
abstract::Sequence analysis of the 16S ribosomal RNA gene was used to study bacterial diversity of a pristine forest soil and of two cultures of the same soil enriched with cellulolytic bacteria. Our analysis revealed high bacterial diversity in the native soil sample, evidencing at least 10 phyla, in which Actinobacteria, Prot...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2011.12.001
更新日期:2012-04-01 00:00:00
abstract::Bacillus species as a group offer several advantages over other bacteria for protection against root pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. The objectives of this work were to determine the ability of strains of Bacillus to inhibit Fusari...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.03.001
更新日期:2005-06-01 00:00:00
abstract::Plasmids that express an enzymatically active beta-galactosidase in an Escherichia coli delta lac strain were isolated from libraries of recombinant plasmids containing Vibrio cholerae chromosomal DNA. Deletion analysis localized the gene responsible for beta-galactosidase activity on a 3.1-kb DNA fragment, and the ge...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(92)90031-i
更新日期:1992-01-01 00:00:00
abstract::Translation termination in eukaryotes is mediated by two polypeptide chain-release factors, eRF1 and eRF3. eRF1 recognizes stop signals, while eRF3 is a ribosome-dependent and eRF1-dependent GTPase. eRF1 forms a stable complex with eRF3 in vivo and in vitro. In the present study, a variety of truncated forms of Euplot...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.07.005
更新日期:2006-11-01 00:00:00
abstract::The promoter probe mini-Tn5-luxAB-tet was used to create a luxAB transcriptional fusion responding to fluorene in the fluorene utilising bacterium Sphingomonas sp. LB126. The mutant strain, named L-132, was impaired in fluorene utilisation and strongly emitted light upon addition of fluorene to the growth medium. L-13...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(01)01268-2
更新日期:2001-12-01 00:00:00
abstract::Bacteria of the Bacillus cereus group are environmental Gram-positive spore-forming bacteria ubiquitously distributed. Despite the high degree of genetic similarity among the different strains, they show strong phenotypic variability, from mammal or entomopathogen strains to soil-dwelling saprophytes, and from psychro...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2016.07.002
更新日期:2017-05-01 00:00:00
abstract::Ammonia-oxidizing archaea (AOA) and bacteria (AOB) are widely distributed in the natural environment and play crucial roles in the nitrification process and the removal of nitrogen (N). Although planktonic microbial community plays an important role in river biogeochemical cycles, few studies have attempted to address...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2014.08.003
更新日期:2014-10-01 00:00:00
abstract::Pseudomonas mendocina carrying a novel class 1 integron containing an IMP-8 gene was isolated from an inanimate surface in a female ward sanitary facility of the Hospital Infante D. Pedro, Aveiro, Portugal. Hybridization with the integrase gene (intI1) and 16S rDNA revealed that the integron is chromosomally located. ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2010.03.004
更新日期:2010-05-01 00:00:00
abstract::Scedosporium apiospermum, a recently isolated phenol-degrading hyphomycete, was shown to be able to productively utilise the diaryl ester phenylbenzoate as its sole source of carbon and energy. The characterisation of degradation intermediates together with the detection of the corresponding catabolic enzymes in crude...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80077-1
更新日期:1999-07-01 00:00:00
abstract::The role of protein-tyrosine kinases in bacterial polymyxin resistance was assessed by both genetic and biochemical approaches. Each of the two genes, wzc and etk, encoding protein-tyrosine kinases in Escherichia coli, was knocked out by using the PCR-based method of one-step inactivation of chromosomal genes, and the...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.01.003
更新日期:2006-09-01 00:00:00
abstract::Spirochetes have a unique motility system that is characterized by flagellar filaments contained within the outer membrane sheath. Direct evidence using video microscopy has recently been obtained which indicates that these periplasmic flagella (PF) rotate in several spirochetal species. This rotation generates thrust...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/0923-2508(92)90117-7
更新日期:1992-07-01 00:00:00
abstract::Thirty mosquitocidal strains of Bacillus sphaericus isolated from different sources and localities in Brazil were characterized phenotypically and genetically to determine their relationship. Among the strains tested, 93.3% were shown to be resistant to lincomycin, 96.6% to novobiocin, 60% to chloramphenicol and all s...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80032-1
更新日期:1999-03-01 00:00:00
abstract::This project focused on the effects of aflatoxin B1 (AFB1), a food-contaminating mycotoxin produced by fungi, genus Aspergillus, on the release and genetic expression of some important cytokines, i.e., (interleukin-1 alpha (IL-1 alpha), IL-6, tumor necrosis factor-alpha (TNF alpha)) by human monocytes. Monocytes, prei...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80042-4
更新日期:1999-01-01 00:00:00
abstract::Heterotrophic microaerophilic bacteria, a diverse and ubiquitous group, are specialized for growth in O2 limited environments. Until recently, microaerophilic bacteria have been grouped with obligate aerobic organisms whose metabolic rates slow in response to physiological O2 limitation. In contrast, microaerophilic b...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2003.11.001
更新日期:2004-03-01 00:00:00
abstract::(51)V NMR spectroscopy was used for detection and identification of cell-associated vanadate (V(5+)) species after exposure of Phycomyces blakesleeanus mycelium, in exponential phase of growth, to sodium orthovanadate. Complete disappearance of monomer and dimer signals and decreased intensity of the tetramer signal w...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.04.012
更新日期:2016-07-01 00:00:00
abstract::A total of 176 strains of Salmonella enterica representing 116 serotypes were tested for the presence of the T6 epitope of the alpha-1,2-linked N-acetyl-D-glucosamine residue by reaction with a murine monoclonal antibody T6 specific for this structure in the Salmonella Ra core lipopolysaccharide (LPS). All 20 serotype...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90185-d
更新日期:1991-06-01 00:00:00
abstract::Urease biogenesis was monitored in the lactic acid bacterium Streptococcus thermophilus during the growth cycle using in-gel detection and a phenol-hypochloride assay. Zymogram analysis, performed in a non-denaturing polyacrylamide gel, enabled visualization of a complex profile of bands whose number and intensity wer...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.04.005
更新日期:2005-11-01 00:00:00
abstract::Sequences of the most conserved proteins encoded by transposable (pro)phages were used to search recently sequenced Firmicute genomes for candidate transposable prophages. One complete Mu-like prophage, SglyMu-1, was identified, in four copies, in the Syntrophobotulus glycolicus DSM 8271 chromosome sequence. Related p...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.01.008
更新日期:2013-05-01 00:00:00
abstract::Microbial transformation of arsenic species and their interaction with the carbon cycle play a major role in the mobility of this toxic metalloid in the environment. The influence of simple or complex organic substrates on arsenic bio-oxidation was studied using two bacterial strains: one - the arsenivorans strain of ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2019.09.006
更新日期:2020-01-01 00:00:00
abstract::The presence of viral dsRNA (L and M fractions) in the cryophilic yeast Saccharomyces bayanus var. uvarum is documented here for the first time. Sixty-eight strains of different origins were analyzed. Most of them did not carry dsRNA; the L fraction was found in seven strains, while 11 strains had both L and M fractio...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2007.07.008
更新日期:2007-10-01 00:00:00
abstract::Although it seems obvious that fever has some important general adaptive value, it is still not clear by what means this function is manifested. One postulate is that febrile conditions result in the sequestration of soluble iron, effectively starving some pathogens of that essential nutrient. On the basis of our rece...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/0923-2508(94)90182-1
更新日期:1994-05-01 00:00:00
abstract::KlADH4 is a gene of Kluyveromyces lactis encoding a mitochondrial alcohol dehydrogenase activity, which is specifically induced by ethanol and insensitive to glucose repression. In this work, we report the molecular analysis of UAS(E), an element of the KlADH4 promoter which is essential for the induction of KlADH4 in...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(00)00131-5
更新日期:2000-01-01 00:00:00
abstract::Ninety-nine strains of Gram-negative black-pigmented anaerobic rods, grown on Todd-Hewitt blood agar plates, were identified and characterized according to a typing scheme including UV fluorescence, catalase, trypsin-like and haemagglutinating activities, biochemical tests with the ATB 32A kit, and gas-liquid chromato...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(93)90051-3
更新日期:1993-07-01 00:00:00
abstract::Helicobacter spp. have been the focus of considerable research because of the role of this genus in gastrointestinal diseases. We infected NIH germ-free mice with Helicobacter trogontum, a recently described intestinal bacterium of rats, in order to study the distribution of this bacterium in the gastrointestinal trac...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80037-0
更新日期:1999-04-01 00:00:00