Abstract：
:KlADH4 is a gene of Kluyveromyces lactis encoding a mitochondrial alcohol dehydrogenase activity, which is specifically induced by ethanol and insensitive to glucose repression. In this work, we report the molecular analysis of UAS(E), an element of the KlADH4 promoter which is essential for the induction of KlADH4 in the presence of ethanol. UAS(E) contains five stress response elements (STREs), which have been found in many genes of Saccharomyces cerevisiae involved in the response of cells to conditions of stress. Whereas KlADH4 is not responsive to stress conditions, the STREs present in UAS(E) seem to play a key role in the induction of the gene by ethanol, a situation that has not been observed in the related yeast S. cerevisiae. Gel retardation experiments showed that STREs in the KlADH4 promoter can bind factor(s) under non-inducing conditions. Moreover, we observed that the RAP1 binding site present in UAS(E) binds KlRap1p.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Mazzoni C,Santori F,Saliola M,Falcone Cdoi
10.1016/s0923-2508(00)00131-5subject
Has Abstractpub_date
2000-01-01 00:00:00pages
19-28issue
1eissn
0923-2508issn
1769-7123pii
S0923-2508(00)00131-5journal_volume
151pub_type
杂志文章abstract：:Genome analyses have revealed that the genomes of non-photosynthetic bacteria including Bacillus subtilis code for proteins similar to the large subunit of RuBisCO (called RuBisCO-like protein (RLP)). This raises a fundamental question as to their functional relationship to photosynthetic RuBisCO. Recently, we identif...
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doi：10.1016/j.resmic.2005.06.009
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doi：10.1016/0923-2508(93)90048-7
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