Abstract：
:The interest of probiotics as remedies for a broad number of gastrointestinal and other infectious diseases has gained wide interest over the last few years, but little is known about their underlying mechanism of action. In this study, the probiotic activities of a human isolate of Lactobacillus casei subsp. rhamnosus strain (Lcr35) were investigated. Using intestinal Caco-2 cell line in an in vitro model, we demonstrated that this strain exhibited adhesive properties. The inhibitory effects of Lcr35 organisms on the adherence of three pathogens, enteropathogenic Escherichia coli (EPEC), enterotoxigenic E. coli (ETEC) and Klebsiella pneumoniae, were determined. A decrease in the number of adhering pathogens was observed, using either preincubation, postincubation or coincubation of the pathogens with Lcr35. Moreover, the antibacterial activities of cell-free Lcr35 supernatant was examined against nine human pathogenic bacteria, ETEC, EPEC, K. pneumoniae, Shigella flexneri, Salmonella typhimurium, Enterobacter cloacae, Pseudomonas aeruginosa, Enterococcus faecalis and Clostridium difficile. The growth of all strains was inhibited, as measured by determining the number of viable bacteria over time, but no bactericidal activity was detected in this in vitro assay. Together, these findings suggest that this probiotic strain could be used to prevent colonization of the gastrointestinal tract by a large variety of pathogens.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Forestier C,De Champs C,Vatoux C,Joly Bdoi
10.1016/s0923-2508(01)01188-3subject
Has Abstractpub_date
2001-03-01 00:00:00pages
167-73issue
2eissn
0923-2508issn
1769-7123pii
S0923250801011883journal_volume
152pub_type
杂志文章abstract：:The trehalose operon of Pseudomonas fluorescens ATCC 17400 consists of treP, treA and treR. The gene treP codes for a putative enzyme II subunit of the phosphotransferase system that catalyzes the phosphorylation of trehalose together with its translocation across the cell membrane and treA encodes a putative phosphot...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)01151-7
更新日期：2000-12-01 00:00:00
abstract：:Chinoike Jigoku ("Blood Pond Hell") is located in the hot spring town of Beppu on the southern island of Kyushu in Japan, and is the site of a red-colored acidic geothermal pond. This study aimed to investigate the microbial population composition in this extremely acidic environment and to isolate/characterize acidop...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.04.011
更新日期：2016-09-01 00:00:00
abstract：:During the past twenty-nine years, not a single class of antimicrobial agents has been discovered that has led to new approved human drugs. Despite a dramatic increase in the potency of existing classes, the need for new effective antimicrobial agents continues. The bacterial phosphotransferase system (PTS) offers the...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(92)90089-7
更新日期：1992-06-01 00:00:00
abstract：:There are several occasions when enumeration of Escherichia coli cells is needed. These include examination of urine specimens and water or food samples. Present methods rely on growth in more or less selective media (colony-forming units on agar or the most probable number method using liquid media). Unfortunately, n...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)00222-9
更新日期：2000-09-01 00:00:00
abstract：:We showed in 1988 that there are two strains of Chlamydia psittaci which infect the koala (Phascolarctos cinereus). In order to further investigate the role of these chlamydial strains in pathogenesis, we have attempted to identify genes of koala type I strain chlamydia which are involved in the immunogenic response. ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(97)83872-7
更新日期：1997-06-01 00:00:00
abstract：:Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.08.001
更新日期：2006-04-01 00:00:00
abstract：:This project focused on the effects of aflatoxin B1 (AFB1), a food-contaminating mycotoxin produced by fungi, genus Aspergillus, on the release and genetic expression of some important cytokines, i.e., (interleukin-1 alpha (IL-1 alpha), IL-6, tumor necrosis factor-alpha (TNF alpha)) by human monocytes. Monocytes, prei...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80042-4
更新日期：1999-01-01 00:00:00
abstract：:To understand antibiotic resistance in Gram-negative bacteria, a key point is to investigate antibiotic accumulation, which is defined by influx and efflux. Several methods exist to evaluate membrane permeability and efflux pump activity, but they present disadvantages and limitations. An optimized spectrofluorimetric...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2017.11.005
更新日期：2018-01-01 00:00:00
abstract：:Subtractive differential hybridization was used to identify genes in Bacillus subtilis that are induced by nutrient limitation. Several transcription units were identified. They exhibited increased transcription when cells were deprived of certain nutrients, such as glucose, ammonium, or phosphate, or when cells were ...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(91)90059-j
更新日期：1991-09-01 00:00:00
abstract：:Fungal dimorphism is the capacity of certain species of fungi to grow in the form of budding yeasts or mycelium depending on the environmental conditions. This characteristic is a complex phenomenon that involves modifications of the molecular machinery in response to different environmental signals. Through the use o...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2012.03.002
更新日期：2012-06-01 00:00:00
abstract：:The promoter probe mini-Tn5-luxAB-tet was used to create a luxAB transcriptional fusion responding to fluorene in the fluorene utilising bacterium Sphingomonas sp. LB126. The mutant strain, named L-132, was impaired in fluorene utilisation and strongly emitted light upon addition of fluorene to the growth medium. L-13...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(01)01268-2
更新日期：2001-12-01 00:00:00
abstract：:In this study, the occurrence and diversity of integrons were evaluated in 697 isolates belonging to Enterobacteriaceae and Aeromonas spp. isolated from urban wastewaters. Screening of integrons was performed by dot blot hybridization and intI-positive strains were further characterized. The global prevalence of integ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.10.010
更新日期：2012-02-01 00:00:00
abstract：:The expression of the feruloyl esterase gene faeA, the alpha-glucuronidase gene aguA, the endoxylanase gene xlnB, and the beta-xylosidase gene xlnD from Aspergillus niger on xylose was studied in a wild-type strain and in a CreA mutant. A decrease in expression of all four genes was observed with increasing xylose con...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80053-9
更新日期：1999-05-01 00:00:00
abstract：:Soil is one of the most complex and challenging environments for microbiologists. In fact, although it contains the largest microbial diversity on the planet, the majority of these microbes are still uncharacterized and represent an enormous unexplored reservoir of genetic and metabolic diversity. Metagenomics, the st...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2010.04.010
更新日期：2010-07-01 00:00:00
abstract：:Sub-MIC antibiotics differentially modulate transcription of subsets of genes by unknown mechanisms. Paradoxically, the RNA polymerase inhibitor rifampicin is able to both upmodulate as well as downmodulate transcription when present at sub-MIC levels. In this study, we analyzed DNA sequences required for transcriptio...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.02.003
更新日期：2013-06-01 00:00:00
abstract：:In contrast to bacteria, mitochondria contain only a few ATP binding cassette (ABC) transporters in their inner membrane. The known mitochondrial ABC proteins fall into two major classes that, in the yeast Saccharomyces cerevisiae, are represented by the half-transporter Atm1p and the two closely homologous proteins M...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(01)01204-9
更新日期：2001-04-01 00:00:00
abstract：:Some pathogenic species belonging to the Vibrionaceae family have been shown to regulate virulence through a complicated network of quorum sensing systems. In this study, three kinds of N-acylhomoserine lactone (AHL) signal molecules were detected in Vibrio ichthyoenteri DA3, a pathogen of cultured turbot (Scophthalmu...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.10.004
更新日期：2010-01-01 00:00:00
abstract：:Although this volume is dedicated to honoring François Jacob, I would like my contribution to broaden the context by recalling the background within which the scientists of those times operated. The specific scientific accomplishments of Jacob will certainly be covered by the other contributors who collaborated with h...
journal_title：Research in microbiology
pub_type： 传,历史文章,杂志文章
doi：10.1016/j.resmic.2014.05.027
更新日期：2014-06-01 00:00:00
abstract：:This communication describes the cloning of a 1.8-kb fragment from the genome of the corynephage phi AAU2, which aborts the phage lytic cycle when cloned on a high-copy shuttle vector. The associated phenotype, called Apld (aborting phage lytic development), was revealed by noting the reduced plaque size and lower eff...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(98)80078-8
更新日期：1998-03-01 00:00:00
abstract：:The reproducibility of random amplified polymorphic DNA (RAPD) was tested using two different thermal cyclers and three brands of Taq DNA polymerase. Three different oligonucleotides were used to obtain patterns of amplified fragments from three DNA samples (Escherichia coli, Bacillus subtilis, and Thermococcus littor...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(93)90194-7
更新日期：1993-06-01 00:00:00
abstract：:Bacteria of the Bacillus cereus group are environmental Gram-positive spore-forming bacteria ubiquitously distributed. Despite the high degree of genetic similarity among the different strains, they show strong phenotypic variability, from mammal or entomopathogen strains to soil-dwelling saprophytes, and from psychro...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2016.07.002
更新日期：2017-05-01 00:00:00
abstract：:Bacillus species as a group offer several advantages over other bacteria for protection against root pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. The objectives of this work were to determine the ability of strains of Bacillus to inhibit Fusari...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.03.001
更新日期：2005-06-01 00:00:00
abstract：:Triclosan is an antimicrobial agent found in many consumer products. Triclosan inhibits the bacterial fatty acid biosynthetic enzyme, enoyl-ACP reductase (FabI). Decreased susceptibility to triclosan correlates with ciprofloxacin resistance in several bacteria. In these bacteria, resistance to both drugs maps to genes...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.09.003
更新日期：2007-10-01 00:00:00
abstract：:The role of protein-tyrosine kinases in bacterial polymyxin resistance was assessed by both genetic and biochemical approaches. Each of the two genes, wzc and etk, encoding protein-tyrosine kinases in Escherichia coli, was knocked out by using the PCR-based method of one-step inactivation of chromosomal genes, and the...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.01.003
更新日期：2006-09-01 00:00:00
abstract：:Translation termination in eukaryotes is mediated by two polypeptide chain-release factors, eRF1 and eRF3. eRF1 recognizes stop signals, while eRF3 is a ribosome-dependent and eRF1-dependent GTPase. eRF1 forms a stable complex with eRF3 in vivo and in vitro. In the present study, a variety of truncated forms of Euplot...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.07.005
更新日期：2006-11-01 00:00:00
abstract：:Paralytic shellfish toxins (PSTs) are sodium channel blocking (SCB) toxins, produced by cyanobacteria, as well as by marine dinoflagellates and their associated bacteria, and cause serious health and economic concern worldwide. In a previous study, approximately 70% of the bacteria enriched from PST-contaminated shell...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(02)01328-1
更新日期：2002-07-01 00:00:00
abstract：:Tellurite-containing media are widely used for the screening and isolation of Shiga toxin-producing Escherichia coli (STEC) O157:H7, but tellurite resistance among non-O157 STEC is poorly characterized. Therefore, we investigated 202 STEC strains representing 61 different serotypes from humans, animals or food for the...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.10.007
更新日期：2007-03-01 00:00:00
abstract：:Lactobacillus plantarum LA 318 is a potential probiotic strain isolated from normal human intestinal tissue that shows high adhesion to human colonic mucin mediated by the bacterial cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We report the adhesion mechanism of the lactobacilli is in part due to GAP...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.07.005
更新日期：2008-11-01 00:00:00
abstract：:Cell-free extracts of various strains belonging to Mycobacterium paratuberculosis (Ptb) and "wood-pigeon" (WP) mycobacteria were compared by polyacrylamide gel electrophoresis and the various protein bands obtained were tested for peroxidase enzyme activity. One strain of Mycobacterium avium served as a control. Bacte...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(90)90019-m
更新日期：1990-06-01 00:00:00
abstract：:(51)V NMR spectroscopy was used for detection and identification of cell-associated vanadate (V(5+)) species after exposure of Phycomyces blakesleeanus mycelium, in exponential phase of growth, to sodium orthovanadate. Complete disappearance of monomer and dimer signals and decreased intensity of the tetramer signal w...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.04.012
更新日期：2016-07-01 00:00:00
