Abstract：
:Azo dye derivatives of azobenzene constitute the largest group of dyes used in the textile industry and possess recalcitrant chemical groups, such as those of azo and sulphonic acid. Some microorganisms are able to degrade these aromatic compounds. In the present work, decolourisation of culture media containing azo dyes by the ligninolytic fungus Phanerochaete chrysosporium was achieved under nitrogen-limited conditions. The dyes used in the study are derivatives of meta- or para-aminosulphonic or aminobenzoic acids and include in their structures groups such as guaiacol or syringol, which are bioaccessible to the lignin degrading fungus P. chrysosporium. The aim of this study was to pre-adapt the microorganism to the structure of the dyes and to establish the relationships of the chemical structure of the dye present in the pre-adaptation medium with the chemical structure of the dye to be degraded. The azo dye used in the pre-adaptation medium that gave the best overall decolourisation performance was a meta-aminosulphonic acid and guaiacol derivative. The azo dye derivative of a meta-aminobenzoic acid and syringol showed a better performance in the decolourisation assays. Preliminary GC-MS studies indicated the formation of a nitroso substituted catechol metabolite, a precursor of aromatic ring cleavage, which was confirmed to occur by an enzymatic assay. The presence of this type of metabolite allows the establishment of a possible metabolic pathway towards mineralisation.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Martins MA,Queiroz MJ,Silvestre AJ,Lima Ndoi
10.1016/s0923-2508(02)01332-3subject
Has Abstractpub_date
2002-07-01 00:00:00pages
361-8issue
6eissn
0923-2508issn
1769-7123pii
S0923-2508(02)01332-3journal_volume
153pub_type
杂志文章abstract：:Plasmids that express an enzymatically active beta-galactosidase in an Escherichia coli delta lac strain were isolated from libraries of recombinant plasmids containing Vibrio cholerae chromosomal DNA. Deletion analysis localized the gene responsible for beta-galactosidase activity on a 3.1-kb DNA fragment, and the ge...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90031-i
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abstract：:Spirochetes have a unique motility system that is characterized by flagellar filaments contained within the outer membrane sheath. Direct evidence using video microscopy has recently been obtained which indicates that these periplasmic flagella (PF) rotate in several spirochetal species. This rotation generates thrust...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(92)90117-7
更新日期：1992-07-01 00:00:00
abstract：:Total genomic DNA isolated by concentrating seawater and mercury-resistant bacteria were hybridized with a mer probe to detect the presence of homologous DNA sequences in marine coastal waters of the Bay of Bengal, India. Coastal water extracts induced with mercury hybridized with the mer operon of transposon Tn501. M...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90026-k
更新日期：1992-03-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.01.009
更新日期：2013-05-01 00:00:00
abstract：:While the scientific contributions of François Jacob were outstanding, I also consider that his conception of science, and of its place among other forms of knowledge, is also highly original, and important for the future of science in our societies. His contributions to the history and philosophy of science were neit...
journal_title：Research in microbiology
pub_type： 传,历史文章,杂志文章
doi：10.1016/j.resmic.2014.05.001
更新日期：2014-06-01 00:00:00
abstract：:Some of the present problems in ribotyping are associated with a lack of uniform reactivity of probes when bacterial DNAs are of phylogenetically diverse origins. To overcome these problems, a set of five oligonucleotides (referred to as OligoMix5) was selected to react with conserved sequences located near both extre...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(99)80064-3
更新日期：1997-11-01 00:00:00
abstract：:There are several occasions when enumeration of Escherichia coli cells is needed. These include examination of urine specimens and water or food samples. Present methods rely on growth in more or less selective media (colony-forming units on agar or the most probable number method using liquid media). Unfortunately, n...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)00222-9
更新日期：2000-09-01 00:00:00
abstract：:Species of Alicyclobacillus, Bacillus and Thermus genera were selected in order to study the possible presence of the (ADP-ribosyl)ation system. These bacteria are thermophilic, aerobic, and were isolated from different geothermal sources. Both activity and expression of (ADP-ribosyl)ating proteins were tested in cell...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.01.004
更新日期：2006-07-01 00:00:00
abstract：:Differential immunoproteomics was applied to identify proteins secreted by Metarhizium anisopliae induced by the Rhipicephalus microplus cuticle. In addition, IgG anti-spore surface proteins were used for searching for proteins possibly involved in early stages of fungus versus tick infection. LC-MS/MS of differential...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.09.012
更新日期：2009-12-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.04.007
更新日期：2004-07-01 00:00:00
abstract：:The use of plasmid pKK3535 (a pBR322-derived plasmid containing a ribosomal RNA operon) is described for determining rRNA gene restriction patterns. The plasmid allowed the use of both radioactive as well as nonradioactive procedures and provided features that are necessary for the inclusion of internal and/or externa...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90024-7
更新日期：1989-05-01 00:00:00
abstract：:RNase E is an endoribonuclease and plays a central role in RNA metabolism. Cyanobacteria, as ancient oxygen-producing photosynthetic bacteria, also contain RNase E homologues. Here, we introduced mutations into the S1 subdomain (F53A), the 5'-sensor subdomain (R160A), and the DNase I subdomain (D296A) according to the...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2020.06.001
更新日期：2020-01-01 00:00:00
abstract：:A total of 28 species of Legionella could be differentiated by rRNA gene restriction patterns generated after cleavage of total DNA with either EcoRV or HindIII restriction endonucleases, and hybridization of fragments with 32P-labelled Escherichia coli 16 + 23S rRNA. Different species gave different fragment patterns...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90193-9
更新日期：1989-11-01 00:00:00
abstract：:Formation of organized colony morphology is clearly a result of organized, coordinated behavior of cells within a colony, which reflects changes in the cell environment, nutrient availability, inter- and intracolony signaling and others. Under standard conditions, colony morphology is specific to the particular yeast ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.05.012
更新日期：2005-11-01 00:00:00
abstract：:Ferric reductase catalyzes the reduction of ferric iron into ferrous iron and plays an essential role in high-affinity iron acquisition. In this study, we found that the cfl1Δ/Δ (orf19.1263) mutant was not defective in iron acquisition. However, deletion of CFL1 increased cellular iron accumulation by elevating surfac...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.03.001
更新日期：2014-04-01 00:00:00
abstract：:The rRNA gene restriction patterns of 89 Vibrio cholerae O1 isolates from different geographic origins were studied. The probe was Escherichia coli 16 + 23S rRNA labelled with "ECL Gene detection system". A total of 17 rRNA gene restriction patterns were observed after BglI cleavage. Four patterns (B1 to B4) were only...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(90)90059-y
更新日期：1990-07-01 00:00:00
abstract：:Heterotrophic microaerophilic bacteria, a diverse and ubiquitous group, are specialized for growth in O2 limited environments. Until recently, microaerophilic bacteria have been grouped with obligate aerobic organisms whose metabolic rates slow in response to physiological O2 limitation. In contrast, microaerophilic b...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2003.11.001
更新日期：2004-03-01 00:00:00
abstract：:The endo-N-acetyl-beta-D-glucosaminidases (ENGase) acting on the N-N'-diacetylchitobiosyl core of N-glycosylproteins are essential reagents for the investigation of the structure and the functions of glycoproteins. These enzymes were largely studied with the aim of offering more tools with new and broader substrate sp...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(96)80289-0
更新日期：1995-07-01 00:00:00
abstract：:Ammonia-oxidizing archaea (AOA) and bacteria (AOB) are widely distributed in the natural environment and play crucial roles in the nitrification process and the removal of nitrogen (N). Although planktonic microbial community plays an important role in river biogeochemical cycles, few studies have attempted to address...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.08.003
更新日期：2014-10-01 00:00:00
abstract：:FliA is known to be a sigma factor that regulates bacterial flagella gene expression. Accumulating evidence suggests that FliA is involved in bacterial behavior other than motility. To elucidate the contribution of FliA to Pseudomonas aeruginosa pathophysiology, we analyzed the biological properties and gene expressio...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2018.02.001
更新日期：2018-04-01 00:00:00
abstract：:The effects of tunicamycin, an inhibitor of N-asparagine-linked glycosylation, on the secretion, adhesion and activities of the cellulase complex produced by Clostridium cellulolyticum have been studied. Tunicamycin at 0.1 micrograms/ml slightly inhibited growth on cellobiose. Endoglucanase, p-nitrophenylcellobiosidas...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)80281-6
更新日期：1995-03-01 00:00:00
abstract：:Mrd1 is one of the trans-acting proteins and plays an important role in precursor ribosomal RNA processing. Here we characterized the Mamrd1 gene from Metarhizium acridum and studied its function in growth, conidiation and virulence using RNA interference. The Mamrd1 gene was identified as participating in the process...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.05.004
更新日期：2011-09-01 00:00:00
abstract：:Klebsiella pneumoniae is one of the major Gram-negative bacterial pathogens causing hospital-acquired multidrug-resistant infections, and the antimicrobial treatment options are scarce. The lack of available antimicrobials has prompted the development of alternative strategies for the treatment of these infections. In...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2019.01.003
更新日期：2019-01-01 00:00:00
abstract：:Mesophilic iron and sulfur-oxidizing acidophiles are readily found in acid mine drainage sites and bioleaching operations, but relatively little is known about their activities at suboptimal temperatures and in cold environments. The purpose of this work was to characterize the oxidation of elemental sulfur (S(0)), te...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.08.022
更新日期：2009-12-01 00:00:00
abstract：:Sporulation histidine kinases, which sense sporulation-specific signals and initiate phosphorelay reactions, are poorly conserved among Bacillus species. We found several putative genes for sporulation histidine kinases in the genome sequence of Paenibacillus polymyxa E681 and assayed the genes for complementation of ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2012.02.003
更新日期：2012-05-01 00:00:00
abstract：:Nisin production by Lactococcus lactis subsp. lactis has been investigated using lactose as carbon source. Whether or not continuous cultures were lactose-limited, maximum nisin titre was observed at an intermediate mu value with a sharp peak of activity between 0.2 and 0.3/h. The maximum specific growth rate obtained...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90075-y
更新日期：1992-11-01 00:00:00
abstract：:The response of Acidithiobacillus ferrooxidans ATCC 23270 to copper was analyzed in sulfur-grown cells by using quantitative proteomics. Forty-seven proteins showed altered levels in cells grown in the presence of 50 mM copper sulfate. Of these proteins, 24 were up-regulated and 23 down-regulated. As seen before in fe...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.07.005
更新日期：2014-11-01 00:00:00
abstract：:Bacteria of the genus Klebsiella are important opportunistic pathogens responsible for nosocomial infections that are increasingly resistant to antimicrobial agents. Distinctive identification of the species K. oxytoca, K. pneumoniae, K. planticola, K. ornithinolytica and K. terrigena is difficult based on phenotypic ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(03)00148-7
更新日期：2003-10-01 00:00:00
abstract：:The increase in genetic variability of a population can be selected during adaptation, as demonstrated by the selection of mutator alleles. The dynamics of this phenomenon, named second-order selection, can result in an improved adaptability of bacteria through regulation of all facets of mutation and recombination pr...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(00)01163-3
更新日期：2001-01-01 00:00:00
abstract：:Translation termination in eukaryotes is mediated by two polypeptide chain-release factors, eRF1 and eRF3. eRF1 recognizes stop signals, while eRF3 is a ribosome-dependent and eRF1-dependent GTPase. eRF1 forms a stable complex with eRF3 in vivo and in vitro. In the present study, a variety of truncated forms of Euplot...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.07.005
更新日期：2006-11-01 00:00:00
