Abstract：
:Cholecystokinins in rat cerebral cortex were studied by radioimmunoassay using an antiserum specific for the COOH-terminus of CCK8. Total concentrations of immunoreactive CCK in cortex were 3-4 fold higher than in jejunum. Rat cerebral CCK was purified by immuno-affinity adsorption to the IgG fraction of CCK antisera conjugated to Sepharose beads, and by gel filtration and ion exchange chromatography. Over 90% of immunoreactive CCK in cortex was accounted for by a factor with the properties of synthetic CCK8, and by a closely related slightly less acidic peptide. In contrast, intestinal CCK consisted of about equal amounts of CCK8-like activity and a larger less acidic immunoreactive component. In both cortex and intestine CCK8-like activity was obtained in substantially higher yield after extracting at neutral pH in boiling water than after extracting in either 0.2 M HCl or 0.5 M acetic acid. However, the larger molecular weight forms of CCK in the intestine were recovered in similar yield by acid and neutral extraction. The principal large form of rat CCK was distinguishable from porcine CCK33 on the basis of both optimum extraction conditions and chromatographic properties. It is suggested that the different distribution of immunoreactive CCK in rat brain and gut can be explained in terms of different biosynthetic processing pathways.
journal_name
Brain Resjournal_title
Brain researchauthors
Dockray GJdoi
10.1016/0006-8993(80)90564-8subject
Has Abstractpub_date
1980-04-21 00:00:00pages
155-65issue
1eissn
0006-8993issn
1872-6240pii
0006-8993(80)90564-8journal_volume
188pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:We electrically stimulated individual Mauthner (M-) cells to determine their motor contribution to C-starts of swimming goldfish. In comparison with sensory-evoked C-starts, M-reflexes triggered by electrical stimulation of single M-cells were significantly weaker and less variable. Stage 1 turns were both longer in d...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91012-6
更新日期：1990-05-28 00:00:00
abstract：:The neurophysiological correlates of autoinhibition at the terminals of nigrostriatal dopaminergic neurons were studied by measuring the changes in antidromic excitability of nigrostriatal neurons following local infusions of various catecholamine agonists and antagonists into the neostriatum. Infusions of apomorphine...
journal_title：Brain research
pub_type： 杂志文章
doi：
更新日期：1984-09-10 00:00:00
abstract：:The distribution of creatine kinase BB-isoenzyme in the human central nervous system (CNS) was investigated immunohistochemically and the findings were compared with the distributions of tubulin and astroprotein. Creatine kinase BB-isoenzyme existed both in neurons and astrocytes, and was universally distributed withi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)91338-0
更新日期：1983-04-11 00:00:00
abstract：:L-Tryptophan produces a significant antihypertensive effect in spontaneously hypertensive rats while D-tryptophan does not change blood pressure. Both isomers of tryptophan significantly increase brain serotonin to the same extent at a time when the antihypertensive effect of L-tryptophan is maximal. Thus, the antihyp...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90985-5
更新日期：1984-03-19 00:00:00
abstract：:Rats reared on a diet in which choline is replaced with N-aminodeanol (NADE), undergo > 50% replacement of brain acetylcholine with acetylated NADE, a false cholinergic transmitter. We examined amounts of sleep and wakefulness in 7 littermate pairs of rats fed either NADE-substituted, or a choline control diet for > 1...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90492-6
更新日期：1993-11-26 00:00:00
abstract：:In this study, we examined the immunohistochemical occurrence and distribution of glial cell line-derived neurotrophic factor (GDNF) in autoptic specimens of normal human hippocampus at different ages, from 22 weeks of gestation (w.g.) to adult life. Two different anti-GDNF polyclonal antibodies were used. Western blo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)03377-7
更新日期：2002-02-22 00:00:00
abstract：:A special feature of the extracellular matrix in adult brains of various species is the concentration of certain components around different sub-populations of neurones, giving rise to net-like structures termed perineuronal nets. Recently, some of these components have been identified but the function of these nets h...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01596-6
更新日期：1999-07-10 00:00:00
abstract：:(2E)-(5-Hydroxy-5,7,8,9-tetrahydro-6H-benzo[a][7]annulen-6-ylidene) ethanoic acid (NCS-382) is an antagonist for gamma-hydroxybutyric acid (GHB) at GHB receptor sites. Advantages of using [(3)H]NCS-382 over [(3)H]GHB in radioligand binding studies are that unlike GHB, NCS-382 does not appear to bind to, activate, or i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)02865-8
更新日期：2003-07-25 00:00:00
abstract：:Simulated networks of excitatory and inhibitory neurons have previously been shown to reproduce critical features of experimental data regarding neural coding in V1, such as a positive relationship between thalamic input spike rate and the power of gamma frequency oscillations. This effect, referred to as modulated ga...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2011.08.070
更新日期：2012-01-24 00:00:00
abstract：:The immunocytological study of LH-RH producing neurons was carried out on 3 newborns and 14 fetuses (from 10 to 36 weeks of age). Perikarya and fibers which were immunoreactive to anti-LH-RH IS were revealed by IF or IE in all the hypothalamus beginning with the 13th week. Important variations in neurons staining may ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(77)90992-1
更新日期：1977-06-10 00:00:00
abstract：:The present study using near-infrared spectroscopy (NIRS) examined prefrontal activation associated with maze-solving performance in adult humans. The participants were required to solve a plus-shaped maze, comparable to the one used for pigeons and human children to behaviorally assess planning processes, by moving a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.12.047
更新日期：2011-02-16 00:00:00
abstract：:Parkinson's disease (PD) is the second most common neurodegenerative disease worldwide. It is typically associated with motor symptoms originating from the degeneration of nigrostriatal dopamine (DA) neurons. Early stages of PD have been associated with an alteration in DA production in intestinal DAergic neurons alon...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2019.146460
更新日期：2019-12-15 00:00:00
abstract：:A role for glutamatergic signaling in the nucleus accumbens (NA) in both the expression and extinction of cocaine seeking has been suggested. The effects of extinction following cocaine self-administration on the expression and synaptosomal distribution of GluR1 and NMDAR1 glutamate receptor subunits in the NA shell a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.01.047
更新日期：2009-04-24 00:00:00
abstract：:The dtsz mutant hamster represents a model of primary paroxysmal dystonia, in which dystonic episodes occur in response to stress. Previous examinations demonstrated striatal dysfunctions in dtsz hamsters. In the present study, in situ hybridization was used to examine preproenkephalin and prodynorphin expression as p...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.04.046
更新日期：2004-07-23 00:00:00
abstract：:Heterotopic transplantations of brain vesicles of a quail embryo into a chick embryo were carried out in order to elucidate if CNS anlagen have plasticity in differentiation at the 7-10 somite stage. Quail cells are distinguished from chick cells due to the difference in nuclear morphology. The prosencephalon did not ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90231-y
更新日期：1990-03-12 00:00:00
abstract：:The response of trunk neural crest cells taken from precise levels of the neural axis and cultured together with adjacent somites to Brain-derived neurotrophic factor (BDNF), was examined in cultures grown in a chemically defined medium. In control cultures, the number of neural crest-derived neurons expressing the HN...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(88)90171-x
更新日期：1988-06-01 00:00:00
abstract：:Three priming injections with the D1/D2 dopamine agonist apomorphine permits a challenge with the D2 agonist quinpirole to elicit robust contralateral rotation and ipsilateral striatal Fos expression in 6-hydroxydopamine lesioned rats. Pretreatment with NMDA glutamate antagonists MK-801 or CPP dose-dependently attenua...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02086-8
更新日期：2001-04-06 00:00:00
abstract：:Alcohols and volatile anesthetics modulate the function of cys-loop ligand-gated ion channels, binding to a putative site between transmembrane domains two and three. The extracellular linker between these two domains is important in the transduction of the gating signal from the glycine binding site to the channel ga...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.03.031
更新日期：2007-06-04 00:00:00
abstract：:A comparison of morphological and biochemical characteristics of the D1-type dopamine receptor has been assessed in two experimental tissues, the superior cervical ganglion (SCG) and caudate nucleus of the rat. Correlation of the distribution of this dopaminergic binding site using in vitro autoradiographic localizati...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)91294-7
更新日期：1987-09-22 00:00:00
abstract：:Tourette syndrome (TS) is a childhood-onset tic disorder associated with abnormal development of brain networks involved in the sensory and motor processing. An involvement of immune mechanisms in its pathophysiology has been proposed. Animal models based on active immunization with bacterial or viral mimics, direct i...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2014.04.027
更新日期：2015-08-18 00:00:00
abstract：:In the adult brain, γ-amino butyric acid (GABA) is an inhibitory neurotransmitter, whereas it acts as an excitatory transmitter in the immature brain, and may be involved in morphogenesis. In the present study, we immunohistochemically examined the developmental changes in GABA signaling in the embryonic mouse cervica...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.10.003
更新日期：2012-11-27 00:00:00
abstract：:The distribution of hypocretin-1 (hcrt-1) and hypocretin-2 (hcrt-2) immunoreactivities in the cat brainstem was examined using immunohistochemical techniques. Hcrt-1- and hcrt-2-positive fibers with varicosities were detected in almost all brainstem regions. However, no hcrt-1- or hcrt-2-immunoreactive neuronal somata...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2003.10.004
更新日期：2004-01-09 00:00:00
abstract：:Following single injections of horseradish peroxidase (HRP) in the superior colliculus (SC) and [3H]proline in the striate cortex of rats, a close correspondence was observed in the topographical arrangements of extrastriate cortical fields of HRP retrograde label and of isotope anterograde label. These results suppor...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90318-3
更新日期：1982-06-24 00:00:00
abstract：:The effects of VIP and of peptides of the VIP family: secretin, glucagon, the porcine histidine isoleucine containing peptide (PHI) and the rat hypothalamic growth hormone-releasing hormone (rhGRF) on the cyclic AMP and inositol phosphate contents of isolated rat superior cervical ganglia were investigated. We demonst...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90200-3
更新日期：1986-06-25 00:00:00
abstract：:The enduring effects of postweaning subchronic methylphenidate (MP) treatment and/or previous early preweaning methamphetamine (MA) application on dopamine (DA) fiber density were investigated in multiple cortical and subcortical areas of the gerbil brain. The study aimed to explore three questions: (1) is the develop...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.06.107
更新日期：2007-10-24 00:00:00
abstract：:The intra-axonal transport of horseradish peroxidase (HRP) has been used to trace the nodose ganglion and brain stem projections of a physiologically distinct nerve - the aortic depressor nerve - following electrophysiological identification. Tetramethyl benzidine (TMB) has been used as the substrate for demonstrating...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90553-3
更新日期：1980-04-21 00:00:00
abstract：:When rat striatal membranes, photolabeled with [3H]dopamine under assay conditions similar to those used for dopamine-sensitive adenylate cyclase, were subjected to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, several radioactively labeled bands appeared. Labeling of these bands was reduced in the ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90426-1
更新日期：1981-12-28 00:00:00
abstract：:Classical anatomical depictions of the cervical sympathetic trunk label it as a cholinergic preganglionic structure. We studied the cervical sympathetic trunk of the rat following daily injection for 5 weeks of guanethidine monosulphate, a regimen known to selectively destroy adrenergic neurons outside of the blood-br...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91101-3
更新日期：1989-10-02 00:00:00
abstract：:Partial sciatic nerve ligation in mice caused a marked and persistent decrease in the latency of paw withdrawal from a thermal stimulus only on the ipsilateral side. This thermal hyperalgesia was abolished by repeated intrathecal pretreatment with a specific antibody to brain-derived neurotrophic factor (BDNF), but no...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03666-1
更新日期：2002-12-27 00:00:00
abstract：:The immunohistochemical distribution of substance P (SP) in the hypothalamic suprachiasmatic nucleus (SCN) was studied in adult male and female Djungarian hamsters (Phodopus sungorus) held under either long or short photoperiods. Intact animals were killed by perfusion with a fixative at the middle of the light or dar...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90649-1
更新日期：1994-02-28 00:00:00
