Abstract：
:In this study, we examined the immunohistochemical occurrence and distribution of glial cell line-derived neurotrophic factor (GDNF) in autoptic specimens of normal human hippocampus at different ages, from 22 weeks of gestation (w.g.) to adult life. Two different anti-GDNF polyclonal antibodies were used. Western blot analysis on homogenates of human and rat brain and recombinant human GDNF resulted in differential detection of monomeric and dimeric forms of the proteins. The ABC immunohistochemical technique revealed that in the Ammon's horn, numerous positive cell bodies occurred in the pyramidal layer, the majority of them being present in the proximal CA1 and in CA2. Sparse positive neurons could be observed in the stratum oriens and moleculare. In the fascia dentata many granule cells showed a light punctate staining, whereas more heavily labelled neurons occurred in the polymorphic layer and, occasionally, in the molecular layer. The distribution pattern of GDNF-like immunoreactivity appeared consistently similar throughout life stages from 29 w.g. to adult age. However, intensity of labelling and frequency of neuronal cell bodies was highest in the neonate and decreased in adulthood. The present data provide a comprehensive map of the localization of GDNF-like immunoreactive neurons in the human archicortex at developmental ages and in the mature tissue and represent a first step towards the identification of hippocampal neurons which express the protein and/or are responsive to it. They further suggest that GDNF may play a role in the development of intrahippocampal circuitry and in neuronal function and maintenance throughout life.
journal_name
Brain Resjournal_title
Brain researchauthors
Pina Serra M,Quartu M,Ambu R,Follesa P,Del Fiacco Mdoi
10.1016/s0006-8993(01)03377-7subject
Has Abstractpub_date
2002-02-22 00:00:00pages
138-46issue
1-2eissn
0006-8993issn
1872-6240pii
S0006899301033777journal_volume
928pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Electrophysiological studies suggest that an increase in excitatory amino acid release may occur in the locus coeruleus during opiate withdrawal. The present study examined directly by microdialysis in anesthetized rats the effect of naltrexone-precipitated opiate withdrawal on the efflux of excitatory amino acids in ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90186-4
更新日期：1994-02-04 00:00:00
abstract：:alpha(1)-Adrenoceptors are concentrated in the locus coeruleus (LC) where they appear to regulate various active behaviors but have been difficult to stimulate effectively. The present study examined the behavioral, pharmacological and neural effects of possible stimulation of these receptors with 6-fluoronorepinephri...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.07.049
更新日期：2009-09-29 00:00:00
abstract：:In the developing vertebrate retina, precise coordination of retinal progenitor cell proliferation and cell-cycle exit is essential for the formation of a functionally mature retina. Unregulated or disrupted cell proliferation may lead to dysplasia, retinal degeneration or retinoblastoma. Both cell-intrinsic and -extr...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2007.04.076
更新日期：2008-02-04 00:00:00
abstract：:Cocaine was injected intravenously to non-anesthetized, freely moving adult male rats and caused dose-dependent elevations in plasma adrenocorticotropin (ACTH) levels. The observation that this stimulatory effect was completely abolished by pretreatment with a corticotropin-releasing factor (CRF) antiserum, coupled wi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90953-x
更新日期：1987-10-06 00:00:00
abstract：:Astrocytes cultured from the brains of neonatal rat pups acquire at least two specializations of intramembrane particle distribution: 'assemblies' and gap junctions. The number and appearance of assemblies in the cultured astrocytes is not markedly influenced by the presence or absence of a collagen substrate, and the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90545-0
更新日期：1983-10-03 00:00:00
abstract：:The independent and combined effects of Ca2+, Mg2+, Zn2+, Al3+ and Li+ on [3H]MK-801 binding in human cerebral cortical membranes were studied to further characterize the modulatory effects of metal ions on the N-methyl-D-aspartate (NMDA) receptor-ionophore. Glycine, in the presence of glutamate, significantly intensi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91290-0
更新日期：1989-05-01 00:00:00
abstract：:The ganglion cells are the output neurons of the retina. There is, however, relatively little known about the neurotransmitters used by these cells. In the present study, ganglion cells identified with a ganglion cell-specific monoclonal antibody (AB5) are shown in separate double-label experiments to be gamma-aminobu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91498-9
更新日期：1988-01-26 00:00:00
abstract：:We used triple-labeling immunohistochemistry in rat midbrain sections to identify dopaminergic neurons that contain either one or both of the calcium-binding proteins, calretinin (CR) and calbindin-D28k (CB). Midbrain dopaminergic neurons were immunohistochemically labeled for tyrosine hydroxylase (TH), CR, and CB. In...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01950-2
更新日期：1999-10-30 00:00:00
abstract：:Extracellular ATP can influence cells via activation of P2X purinoceptors, the distribution of which can be altered in the central and peripheral nervous systems following injury or tissue damage. Here we have investigated the effect of a unilateral section of the cervical vagus nerve on the distribution of P2X(1), P2...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)02797-5
更新日期：2003-07-04 00:00:00
abstract：:The responses of single alpha motoneurons to various "natural' stimuli were recorded from dissected ventral root filaments in lightly anesthetized control and recently decerebellate cats. The identity of the motoneurons was established by recording the unitary responses to orthodromic stimulation of nerves innervating...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(75)90208-5
更新日期：1975-09-12 00:00:00
abstract：OBJECTIVE:To investigate the effect of salvinorin A (SA) on brain injury and neurologic function post-brain ischemia/reperfusion (I/R) using a rat forebrain ischemia model and further explore the effect of kappa opioid receptor (KOR) inhibition by SA on aquaporin-4 (AQP4) expression in the hippocampus, cortex and stria...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.02.024
更新日期：2016-04-15 00:00:00
abstract：:Gonadal steroids are thought to act by activation of the genome following cytoplasmic binding, translocation of the hormone--receptor complex to the nucleus and chromatin binding at specific acceptor sites. This study examined the kinetics of chromatin binding of estradiol by hypothalamic and cortical cells of adult g...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(78)90138-5
更新日期：1978-08-18 00:00:00
abstract：:Dexmedetomidine (Dex) has the neuroprotective effect on cerebral ischemia-reperfusion injury (CIRI). But the mechanism is not yet clear. In this study, we established a model of middle cerebral artery occlusion (MCAO) and treated primary cortical neurons with oxygen glucose deprivation (OGD), followed by Dex treatment...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2018.09.007
更新日期：2018-12-15 00:00:00
abstract：:After a moderate global cerebral ischemia, two hypothetical populations of pyramidal neurons are present among the hippocampal CA1 pyramidal neurons: one that will die and another one that will survive. Prior analysis of dissected hippocampal CA1 regions has shown a reduction of the GluR1-3 mRNA following ischemia. In...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)01985-0
更新日期：2001-03-09 00:00:00
abstract：:The expression of adenylyl cyclase (AC) isoforms in the adult rat vestibular periphery was investigated using reverse transcription polymerase chain reaction (RT-PCR). AC II, IV and V mRNAs were expressed in both Scarpa's ganglion and vestibular end organs. In addition, in the vestibular end organs, an AC mRNA not pre...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02007-2
更新日期：2000-03-24 00:00:00
abstract：:The effects of the 8-alpha-amino-ergoline CU 32-085 on central dopamine neuronal systems was investigated. Two h after administration of CU 32-085 a slight increase of dopamine levels was observed in the nucleus caudatus-putamen. Radioligand binding studies in vitro have shown that CU 32-085 has a low affinity for str...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)91044-3
更新日期：1985-03-04 00:00:00
abstract：:In this microdialysis study we measured the extracellular neuropeptide Y (NPY) levels in the dorsal hippocampus of conscious rats. During potassium-induced depolarisations, a 93% increase in extracellular levels of NPY was observed. NPY has been demonstrated to reduce kainic acid-induced convulsions in rats, but it is...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)01374-7
更新日期：1998-01-19 00:00:00
abstract：:The exposure of cultured rat striatal neurons to L-DOPA caused marked cell death. The L-DOPA cytotoxicity was inhibited by the addition of Mg2+ to and by the removal of Ca2+ from the culture medium, and also by the application of tetrodotoxin. Moreover, prolonged application of L-DOPA increased the glutamate content i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00908-6
更新日期：1997-10-10 00:00:00
abstract：:In the present experiment we examined the effect of progesterone upon dopamine (DA) release induced by a direct infusion of unlabeled L-dihydroxyphenylalanine (L-DOPA) into the caudate nucleus of freely behaving rats. Ovariectomized rats were implanted with a push-pull cannula directed at the caudate nucleus and subje...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90150-9
更新日期：1989-08-07 00:00:00
abstract：:The density of protein gene product 9.5- and tyrosine hydroxylase-immunoreactive nerve fibers innervating circumvallate papillae of the tongue was substantially increased in transgenic mice that overexpressed nerve growth factor (NGF) when compared with age-matched controls. The fiber density was age-dependent. Only t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)01336-9
更新日期：1996-01-29 00:00:00
abstract：:The effects of alpha-bungarotoxin from Bungarus multicinctus and Bungarus caeruleus were studied on three types of cholinergic response in Aplysia central neurones. These responses are the result of three distinct changes in ionic permeability: selective increases in permeabiliyt to Na, Cl and K, respectively. It was ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(76)90142-6
更新日期：1976-05-14 00:00:00
abstract：:Fluorescence immunohistochemistry was performed to characterize the distribution and phenotype of GLUT8-positive neurons in rat brain and to compare the cellular distribution of GLUT8 with GLUT3 in the hippocampus. Based upon the absence of co-localization with the non-neuronal markers GFAP (astroglial) and OX42 (micr...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02308-9
更新日期：2002-04-05 00:00:00
abstract：:Variables influencing decision-making in real settings, as in the case of voting decisions, are uncontrollable and in many times even unknown to the experimenter. In this case, the experimenter has to study the intention to decide (vote) as close as possible in time to the moment of the real decision (election day). H...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.06.046
更新日期：2010-09-10 00:00:00
abstract：:The neurons that produce gonadotrophin-releasing hormone (GnRH) are mainly found in the medial preoptic area (MPOA) and constitute a common final pathway to the control of luteinizing hormone (LH) surge on proestrus. The control of GnRH secretion depends on several neurotransmitters, such as serotonin (5-HT), noradren...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.01.045
更新日期：2007-04-20 00:00:00
abstract：:Slices of the molecular layer of the dentate gyrus of the hippocampal formation were incubated with either [14C]glucose, [14C]pyruvate or 14C glutamine and the efflux of endogenous and radioactive glutamate was monitored under various conditions. After prelabeling with either [14C]glutamine or [14C]glucose elevation o...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90306-8
更新日期：1979-06-08 00:00:00
abstract：:Despite the significant advances that have been made in understanding the pathophysiology of cerebral ischemia on the cellular and molecular level, only one drug, the thrombolytic tissue plasminogen activator (rt-PA), is approved by the FDA for use in patients with acute ischemic stroke. Therefore, there is a critical...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.07.061
更新日期：2007-10-10 00:00:00
abstract：:The double-labeling, fluorescent dye technique was used in order to demonstrate the extent to which the central processes of renal afferent neurons directly project to the brainstem. One injection of a fluorescent dye was placed in the cortex of the left kidney of male rats, and a second injection of a different dye w...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)91154-5
更新日期：1984-04-23 00:00:00
abstract：:Cyclooxygenase-2 (COX-2) is the predominant isoform of cyclooxygenase in brain. COX-2 activity produces oxidative stress and results in the production of prostaglandins that have many injurious effects. COX-2 transcription is induced by synaptic activity; therefore, COX-2 activity could contribute to epileptic neurona...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.05.038
更新日期：2005-07-19 00:00:00
abstract：:Nitric oxide (NO), a potent vasodilator and nontraditional neurotransmitter, is an important mediator of the changes in cerebral blood flow (CBF) associated with increased neuronal activity (neurovascular coupling). In the present work, we investigated the role of NO and of its newly recognized precursor, nitrite, in ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2011.06.045
更新日期：2011-08-17 00:00:00
abstract：:The mismatch negativity is an electrophysiological marker of auditory change detection in the event-related brain potential and has been proposed to reflect an automatic comparison process between an incoming stimulus and the representation of prior items in a sequence. There is evidence for two main functional subcom...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.07.079
更新日期：2008-07-18 00:00:00
