Chromatin binding of estradiol in the hypothalamus and cortex of male and female rats.

Abstract:

:Gonadal steroids are thought to act by activation of the genome following cytoplasmic binding, translocation of the hormone--receptor complex to the nucleus and chromatin binding at specific acceptor sites. This study examined the kinetics of chromatin binding of estradiol by hypothalamic and cortical cells of adult gonadectomized male and female rats. In both sexes binding was found to be limited to hypothalamic cells. The kinetics of binding differed between the sexes, estradiol being retained less well in males. In females hypothalamic chromatin binding was shown to be of limited capacity and to be competitively inhibited by unlabeled estradiol. Unlabeled testosterone inhibited estradiol binding when administered subcutaneously 3 h before the estradiol, but not by testosterone when administered either intravenously or concurrently with the estradiol. It was suggested that the observed sex difference in hypothalamic chromatin binding might underlie the relative insensitivity of the male to the lordosis-inducing properties of estradiol.

journal_name

Brain Res

journal_title

Brain research

authors

Whalen RE,Olsen KL

doi

10.1016/0006-8993(78)90138-5

subject

Has Abstract

pub_date

1978-08-18 00:00:00

pages

121-31

issue

1

eissn

0006-8993

issn

1872-6240

pii

0006-8993(78)90138-5

journal_volume

152

pub_type

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