Abstract：
:The binding interactions of three naphthalimide derivatives with heteropoly nucleic acids have been evaluated using fluorescence, absorption and circular dichroism spectroscopies. Mono- and bifunctionalized naphthalimides exhibit sequence-dependent variations in their affinity toward DNA. The heteropoly nucleic acids, [Poly(dA-dT)]2 and [Poly(dG-dC)]2, as well as calf thymus (CT) DNA, were used to understand the factors that govern binding strength and selectivity. Sequence selectivity was addressed by determining the binding constants as a function of polynucleotide composition according to the noncooperative McGhee-von Hippel binding model. Binding affinities toward [poly(dA-dT)](2) were the largest for spermine-substituted naphthalimides (Kb = 2-6 x 10(6) M(-1)). The association constants for complex formation between the cationic naphthalimides and [poly(dG-dC)]2 or CT DNA (58% A-T content) were 2-500 times smaller, depending on the naphthalimide-polynucleotide pair. The binding modes were also assessed using a combination of induced circular dichroism and salt effects to determine whether the naphthalimides associate with DNA through intercalative, electrostatic or groove-binding. The results show that the monofunctionalized spermine and pyridinium-substituted naphthalimides associate with DNA through electrostatic interactions. In contrast, intercalative interactions are predominant in the complex formed between the bifunctionalized spermine compound and all of the polynucleotides.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
McMasters S,Kelly LAdoi
10.1111/j.1751-1097.2007.00155.xsubject
Has Abstractpub_date
2007-07-01 00:00:00pages
889-96issue
4eissn
0031-8655issn
1751-1097pii
PHP155journal_volume
83pub_type
杂志文章abstract：:Generating accurate population-specific public health messages regarding sun protection requires knowledge about seasonal variation in sun exposure in different environments. To address this issue for a subtropical area of Australia, we used polysulphone badges to measure UVR for the township of Nambour (26 degrees la...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2009.00686.x
更新日期：2010-03-01 00:00:00
abstract：:The progressive loss of visual faculties with advancing age is attributed, sometimes, mainly to allegedly inevitable optical deterioration, such as clouding of the media, or again to neurosenescence, as typified by cell-death. The relevant factors are assessed, and it is not thought likely that changes in normal ocula...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.1989.tb04181.x
更新日期：1989-09-01 00:00:00
abstract：:Although histochemical and immunohistochemical methods are the standard procedures in diagnosis of lymphoproliferative disorders, useful improvements in evidencing histopathologic manifestations can be obtained with the introduction of tissue autofluorescence analyses. We used microspectrofluorometry and a Multispectr...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)071<0737:miamft>2.0.co;2
更新日期：2000-06-01 00:00:00
abstract：:This paper reviews briefly the evidence for an association between various measures of UV radiation (UVR) exposure and the development of cancer. Issues such as data quality, study design, measurement variation, comparability of studies, and quantification of UV exposure in relation to skin cancer are discussed. A ran...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1562/2005-02-18-IR-445
更新日期：2005-11-01 00:00:00
abstract：:The cellular uptake, localization and efflux of meso-tetra-(4-hydroxyphenyl)porphyrin (p-THPP)-loaded nanoparticles have been studied in EMT-6 tumor cells. The effect of blood serum on photocytotoxicity has also been evaluated. Sub-130 nm nanoparticles based on poly(D,L-lactide-co-glycolide) (PLGA) (50:50 PLGA and 75:...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2003)077<0638:eopinc>2.0.co;2
更新日期：2003-06-01 00:00:00
abstract：:The kinetics of transients formed under photoexcitation of benzophenone (B) dissolved in three different polymers was studied by ns laser flash photolysis. These polymers were the soft rubbers poly (ethylene-co-butylene) (EB), polystyrene block-poly(ethylene-ran-butylene)-block-polystyrene (SEBS) and hard polystyrene ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12170
更新日期：2014-03-01 00:00:00
abstract：:A noninvasive in situ fluorescence-based method for the quantification of the photosensitizer chloroaluminum disulfonated phthalocyanine was compared to the highly accurate but nonreal time ex vivo spectrofluorometry method. Our in vivo fluorescence technique is designed to allow real-time assessment of photosensitize...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2001)074<0453:copaqt>2.0.co;2
更新日期：2001-09-01 00:00:00
abstract：:A solution of iodouracil (0.2 mg/mL) and KI (0.1 M) in 0.23 M borate buffer (pH 7) forms triiodide upon exposure to UVB. In the presence of thyodene, the blue starch-iodine complex is formed, the intensity of which is proportional to the amount of UVB exposure. Studies were conducted using either a sunlamp or solar ir...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-08-01 00:00:00
abstract：:The retinal pigment epithelium (RPE) is a highly metabolic layer of postmitotic cells lining Bruch's membrane in the retina. While these cells contain endogenous photosensitizers that mediate blue light-induced damage, it has also been shown that blue light exposure damages mitochondrial DNA in RPE cells resulting in ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12683
更新日期：2017-03-01 00:00:00
abstract：:We present an in vivo study of the optical properties of melanin present in melanocytic nevi of human subjects with Fitzpatrick skin type III (Caucasian descent) using optical spectroscopy. We show that the melanin absorption spectrum exhibits an exponential dependence on wavelength with a decay constant which follows...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2008.00436.x
更新日期：2009-01-01 00:00:00
abstract：:The activity of the bimodal fluorescent protein (bmFP) (lambda max, 488 and 517 nm) in the in vitro luciferase reaction has been studied. The bmFP that is produced by Photobacterium phosphoreum strain bmFP is a dimer of two homologous subunits binding four riboflavin 5'-phosphate (FMN)-myristate chromophores. The addi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)071<0237:aotbfp>2.0.co;2
更新日期：2000-02-01 00:00:00
abstract：:In aqueous solution, amphiphilic ionenes such as the [3,22]-ionene spontaneously adopt globular conformations and form microdomains that are highly micelle-like, i.e. are capable of solubilizing organic molecules, binding and exchanging counterions and accelerating or inhibiting the rates of bimolecular reactions. Tim...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2006-09-09-RA-1036
更新日期：2007-05-01 00:00:00
abstract：:The wavelength dependence of an ultraviolet irradiation of the DNA substrate for a human endonuclease was determined. Sites of DNA incision for all UVB and UVC wavelengths examined were at cytosines which were neither cyclobutane pyrimidine dimers nor 6-4'-(pyrimidin-2-one)pyrimidines. The optimal wavelengths for form...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1989.tb04120.x
更新日期：1989-03-01 00:00:00
abstract：:Carp (Cyprinus carpio) were repeatedly exposed to 0, 60, 120 and 240 mJ/cm2 ultraviolet B (UVB) radiation three times in 1 week (short-term exposure) or 12 times in 4 weeks (long-term exposure). The effect of UVB on the functioning of the carp immune system was studied on day 2 after the final irradiation. After short...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-07-13-RA-231
更新日期：2005-05-01 00:00:00
abstract：:This study compares the abilities of the glutathione (GSH) and thioredoxin (Trx) antioxidant systems in defending cultured human lens epithelial cells (LECs) against UVA light. Levels of GSH were depleted with either L-buthionine-(S,R)-sulfoximine (BSO) or 1-chloro-2,4-dinitrobenzene (CDNB). CDNB treatment also inhibi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12404
更新日期：2015-03-01 00:00:00
abstract：:A new type of photochromic molecule that can be used for diffusion coefficient (D) measurements of various proteins in solution is described. The absorption spectrum of this molecule is changed upon photoexcitation by the trans-cis isomerization reaction. Target proteins were labeled by this photochromic molecule in t...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2008.00315.x
更新日期：2008-07-01 00:00:00
abstract：:Gel sequencing experiments with the 5'- and 3'-end-labeled oligonucleotides d(A3GA4GA5GA6GA3G) and d(AT)10 have demonstrated that dimeric adenine photoproducts and thymine-adenine photoadducts constitute alkali-labile lesions in UV-irradiated DNA. On treatment with hot piperidine, DNA strand breakage occurs predominan...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05022.x
更新日期：1994-02-01 00:00:00
abstract：:alpha-Crystallin, a major protein of the mammalian lens, plays a vital role in maintaining the structural stability and transparency of the lens. It performs this function through chaperone-like activity; it has recently been reported that heating alpha-crystallin enhances this ability. The present studies, using both...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)071<0470:haceot>2.0.co;2
更新日期：2000-04-01 00:00:00
abstract：:Aspects of different calibration procedures for erythemally weighing broadband radiometers are presented in this study. These instruments are common in projects dealing with ultraviolet radiation effects on humans. Many erythemally weighing broadband radiometers are still operated using a single calibration factor (cf...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2003)078<0603:SCFBSU>2.0.CO;2
更新日期：2003-12-01 00:00:00
abstract：:High-altitude Andean lakes (HAAL) are a treasure chest for microbiological research in South America. Their indigenous microbial communities are exposed to extremely high UV irradiation and to multiple chemical extremes (Arsenic, high salt content, alkalinity). Microbes are found both, free-living or associated into m...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.12555
更新日期：2016-01-01 00:00:00
abstract：:It has previously been demonstrated that chronic low-dose solar-simulated UV radiation could induce both local and systemic immunosuppression as well as tolerance to a topically applied hapten. In this study, we have used a chronic low-dose UV-irradiation protocol to investigate the effects of UVA on the skin immune s...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1996.tb01863.x
更新日期：1996-12-01 00:00:00
abstract：:We present the detailed characterization on the reconstitution of two cyclic tetrapyrrole pigments that have the same substituents but differ in the degree of hydrogenation in the macrocycles from bacteriochlorophyll (BChl) a (7,8,17,18-tetrahydroporphyrin) into the binding sites of B800 BChl a in light-harvesting com...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12919
更新日期：2018-07-01 00:00:00
abstract：:Fibrous proteins discolor on exposure to the UV component of sunlight. This effect is exacerbated in the presence of fluorescent whitening agents (FWAs), which are often applied to textiles to improve product brightness. Tryptophan photoproducts have been identified as significant contributors to protein photoyellowin...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2009.00614.x
更新日期：2009-11-01 00:00:00
abstract：:Eumelanin plays a variety of important physiological roles in human skin. However, its structure and fundamental properties still remain poorly understood. Although the absorbance of eumelanin is broad and reveals little about its structure, a variety of techniques have revealed the presence of a disordered array of c...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00186.x
更新日期：2007-11-01 00:00:00
abstract：:We report the synthesis of twelve indole derivatives bearing nitro or amide groups via Fischer indole methodology followed by reduction/acetylation and amidation reactions. After thorough characterization, these indoles were subjected to a number of studies in order to evaluate their bioactive potential as photosynthe...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13295
更新日期：2020-11-01 00:00:00
abstract：:Detergent solubilized bacteriorhodopsin (BR) proteins which contain alterations made by site-directed mutagenesis (Asp-96----Asn, D96N; Asp-85----Asn, D85N; and Arg-82----Gln, R82Q) have been studied with resonance Raman spectroscopy. Raman spectra of the light-adapted (BRLA) and M species in D96N are identical to tho...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1991.tb03638.x
更新日期：1991-03-01 00:00:00
abstract：:The contribution of DNA strand breaks accumulating in the course of nucleotide excision repair to upregulation of the p53 tumor suppressor protein was investigated in human dermal fibroblast strains after treatment with 254 nm ultraviolet (UV) light. For this purpose, fibroblast cultures were exposed to UV and incubat...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)072<0562:locbds>2.0.co;2
更新日期：2000-10-01 00:00:00
abstract：:The two-photon photolysis of liquid CCl4 with 25 ps pulses of 266 nm light has been studied and compared with similar studies with high energy radiation. Both neutral and ionic species are produced from excited states and ionization. The emphasis of the study is on the ionic processes, while some data related to excit...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2005-06-03-RA-563
更新日期：2006-01-01 00:00:00
abstract：:Photosynthetic bacteria are attractive for biotechnology because they produce no oxygen and so H2 -production is not inhibited by oxygen as occurs in oxygenic photoorganisms. Rhodopseudomonas palustris and Afifella marina containing BChl a can use irradiances from violet near-UV (VNUV) to orange (350-650 nm) light and...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12124
更新日期：2013-09-01 00:00:00
abstract：:These experiments tested the hypothesis that phototropic bending arises when a light gradient across the stem differentially inhibits cell elongation because of direct inhibition of cell elongation by light (the Blaauw hypothesis). Continuous irradiation of dark-grown cucumber seedlings (Cucumis sativus L.) with unil...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1985.tb01642.x
更新日期：1985-01-01 00:00:00
