Abstract:
:The contribution of DNA strand breaks accumulating in the course of nucleotide excision repair to upregulation of the p53 tumor suppressor protein was investigated in human dermal fibroblast strains after treatment with 254 nm ultraviolet (UV) light. For this purpose, fibroblast cultures were exposed to UV and incubated for 3 h in the presence or absence of l-beta-D-arabinofuranosylcytosine (araC) and/or hydroxyurea (HU), and then assayed for DNA strand breakage and p53 protein levels. As expected from previous studies, incubation of normal and ataxia telangiectasia (AT) fibroblasts with araC and HU after UV irradiation resulted in an accumulation of DNA strand breaks. Such araC/HU-accumulated strand breaks (reflecting nonligated repair-incision events) following UV irradiation were not detected in xeroderma pigmentosum (XP) fibroblast strains belonging to complementation groups A and G. Western blot analysis revealed that normal fibroblasts exhibited little upregulation of p53 (approximately 1.2-fold) when incubated without araC after 5 J/m2 irradiation, but showed significant (three-fold) upregulation of p53 when incubated with araC after irradiation. AraC is known to inhibit nucleotide excision repair at both the damage removal and repair resynthesis steps. Therefore, the potentiation of UV-induced upregulation of p53 evoked by araC in normal cells may be a consequence of either persistent bulky DNA lesions or persistent incision-associated DNA strand breaks. To distinguish between these two possibilities, we determined p53 induction in AT fibroblasts (which do not upregulate p53 in response to DNA strand breakage) and in XP fibroblasts (which do not exhibit incision-associated breaks after UV irradiation). The p53 response after treatment with 5 J/m2 UV and incubation with araC was similar in AT, XPA, XPG and normal fibroblasts. In addition, exposure of XPA and XPG fibroblasts to UV (5, 10 or 20 J/m2) followed by incubation without araC resulted in a strong upregulation of p53. We further demonstrated that HU, an inhibitor of replicative DNA synthesis (but not of nucleotide excision repair), had no significant impact on p53 protein levels in UV irradiated and unirradiated human fibroblasts. We conclude that upregulation of p53 at early times after exposure of diploid human fibroblasts to UV light is triggered by persistent bulky DNA lesions, and that incision-associated DNA strand breaks accumulating in the course of nucleotide excision repair and breaks arising as a result of inhibition of DNA replication contribute little (if anything) to upregulation of p53.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Enns L,Murray D,Mirzayans Rdoi
10.1562/0031-8655(2000)072<0562:locbds>2.0.co;2subject
Has Abstractpub_date
2000-10-01 00:00:00pages
562-8issue
4eissn
0031-8655issn
1751-1097journal_volume
72pub_type
杂志文章abstract::The XPA gene-deficient mouse, an animal model of xeroderma pigmentosum (XP), develops enhanced photobiologic reactions including acute inflammation, immunosuppression and skin carcinogenesis, because of the defect in the excision repair of ultraviolet-induced DNA lesions. The results strongly suggest that nuclear DNA ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1562/2006-03-02-IR-829
更新日期:2007-01-01 00:00:00
abstract::We study the fluorescence lifetime of the well-known 1-pyrene butyric acid (PBA) to assess oxygen concentrations in living cells. The behavior of the probe is first studied in water, ethanol, protein solution and liposome suspension. The Stern-Volmer plot of these solutions is linear, and the bimolecular reaction rate...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-03-11-RA-109
更新日期:2004-09-01 00:00:00
abstract::Pyrimidine dimers were measured in epidermal DNA of SKH:HR1 mice following exposure to solar-simulated UV radiation (SSUV, 290-400 nm) or to UVA (320-400 nm). Mice were exposed to SSUV or UVA after topical application (2 mg/cm2) of vehicle, a UVB absorber (5% 2-ethylhexyl p-methoxycinnamate [2-EHMC]), or a broad-spect...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1997.tb07961.x
更新日期:1997-06-01 00:00:00
abstract::We report the preparation of aminopropyl-functionalized magnesium phyllosilicate (Mg-organoclay) conjugated to fluorescein isothiocyanate (FITC) functionality (FITC-organoclay) by one-pot sol-gel synthesis. The physical characteristics of the Mg- and FITC-organoclays were examined by scanning electron microscopy (SEM)...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2009.00701.x
更新日期:2010-05-01 00:00:00
abstract::Magnetic field effect studies of alkylcobalamin photolysis provide evidence for the formation of a reactive radical pair that is born in the singlet spin state. The radical pair recombination process that is responsible for the magnetic field dependence of the continuous-wave (CW) quantum yield is limited to the diffu...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1996.tb02460.x
更新日期:1996-08-01 00:00:00
abstract::We calculated the integrated UVB and plant damage irradiances for Argentina, a country in the Southern Hemisphere spread over a large latitudinal range. The irradiances were calculated for clear sky days using the Madronich code for the average conditions of the months corresponding to the summer and winter solstices ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::Cumulative and intermittent sun exposure are risk factors for skin cancer, highlighting the need to monitor exposure during childhood. There is currently very little available information concerning the accuracy of self-reported levels of sun exposure, particularly for very young children. In this study, UV radiation ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2000)071<0060:cbramu>2.0.co;2
更新日期:2000-01-01 00:00:00
abstract::Cultured cells accumulate acridine orange (AO), which is a weak basic dye and a photosensitizer, in lysosomes and other acidic compartments. During exposure to blue light, AO-loaded macrophages show decreasing red granular fluorescence and increasing green diffuse fluorescence. This is hypothesized to represent peroxi...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1990.tb01685.x
更新日期:1990-01-01 00:00:00
abstract::In this study, we investigated whether the spectrum of p53 mutations in skin tumors induced in hairless SKH-hr1 mice by a solar simulator (290-400 nm) are similar to those found in skin tumors induced in C3H mice by UV radiation from unfiltered (250-400 nm) and Kodacel-filtered (290-400 nm) FS40 sunlamps. Analysis of ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(1998)067<0227:mihshm>2.3.co;2
更新日期:1998-02-01 00:00:00
abstract::This study outlines the potential for Computational Fluid Dynamics (CFD) simulation to be used to predict upper-room ultraviolet germicidal irradiation (UVGI) effectiveness to aid system design and the development of future guidance. A numerical study of two wall-mounted UVGI lamps in a mechanically ventilated test ch...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12013
更新日期:2013-07-01 00:00:00
abstract::Muscle injuries represent ca 30% of sports injuries and excessive stretching of muscle causes more than 90% of injuries. Currently the most used treatments are nonsteroidal anti-inflammatory drugs (NSAIDs), however, in last years, low-level laser therapy (LLLT) is becoming an interesting therapeutic modality. The aim ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2012.01236.x
更新日期:2013-03-01 00:00:00
abstract::Continuous wave and pulsed laser ablation of tissue is described as an explosive event. A subsurface temperature maximum and superheated tissue produce high pressures that eject fragments from the tissue. Decreased water content due to dehydration and vaporization decreases thermal conductivity which reduces heat cond...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/j.1751-1097.1991.tb09896.x
更新日期:1991-06-01 00:00:00
abstract::Differences between global radiation UVER (erythemal ultraviolet solar radiation) received under full sun and diffuse radiation received under the shadow of two types of tree are analyzed to check the importance of these components on human exposure to UV radiation. Blue Line spores dosimeters of VioSpor were used for...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12020
更新日期:2013-05-01 00:00:00
abstract::A set of linear and asymmetric coumarin-imidazole hybrid compounds having a N,N-diethylamine at 7-position and imidazole at 3-position on the coumarin were synthesized. Insertion of thiophene π-spacer between coumarin and imidazole moieties (5b, 5d) leads to redshifted absorption and emission compared to 5a and 5c. Al...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13024
更新日期:2019-05-01 00:00:00
abstract::Exposure to ultraviolet radiation is closely linked to the development of skin cancers in humans. The ultraviolet B (UVB) radiation wavelength (280-320 nm), in particular, causes DNA damage in epidermal keratinocytes, which are linked to the generation of signature premalignant mutations. Interactions between dermal f...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12317
更新日期:2014-11-01 00:00:00
abstract::In cultured human keratinocytes, the tumor suppressor p53 acts as a control element in the protective response to UVB radiation and is affected by a variety of factors linked to cellular adhesion and differentiation. Because keratinocytes within the epidermis are not a homogeneous population but differ in their prolif...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2000)071<0321:pduroh>2.0.co;2
更新日期:2000-03-01 00:00:00
abstract::Pharaonis phoborhodopsin (ppR or sensory rhodopsin II) is a negative phototaxis receptor of Natronomonas pharaonis, and forms a complex, which transmits the photosignal into cytoplasm, with its cognate transducer (pHtrII). We examined a possible local dynamics change of ppR and its D75N mutant complexed with pHtrII, u...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00326.x
更新日期:2008-07-01 00:00:00
abstract::Ultraviolet (UV) radiation is among the most prevalent environmental factors that influence human health and disease. Even 1 h of UV irradiation extensively damages the genome. To cope with resulting deleterious DNA lesions, cells activate a multitude of DNA damage response pathways, including DNA repair. Strikingly, ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13245
更新日期:2020-05-01 00:00:00
abstract::This work evaluated the photoprotective and antigenotoxic effects against ultraviolet B (UVB) radiation of flavonoid compounds apigenin, naringenin and pinocembrin. The photoprotective efficacy of these compounds was estimated using in vitro photoprotection indices, and the antigenotoxicity against UVB radiation was e...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13085
更新日期:2019-07-01 00:00:00
abstract::Excessive exposure of solar ultraviolet (UV) radiation, particularly its UV-B component, to humans causes many adverse effects that include erythema, hyperplasia, hyperpigmentation, immunosuppression, photoaging and skin cancer. In recent years, there is increasing use of botanical agents in skin care products. Pomegr...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-08-06-RA-264
更新日期:2005-01-01 00:00:00
abstract::The effect of cucurbit[7]uril (CB7) on the spiropyran-merocyanine photochromic interconversion was studied in acidic and alkaline aqueous solutions. The merocyanine (MC) isomer was found to be the thermodynamically most stable form both in water and in the presence of CB7. A preferential binding of the protonated mero...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2012.01183.x
更新日期:2012-11-01 00:00:00
abstract::The efficiency of ruthenium complexes for photosensitizing DNA damage depends on the oxidizing character of their ligands. Here we report on the difference in behavior of tris(2.2'-bipyrazyl)ruthenium(II) (Ru[bpz]3(2+)), tris(2,2'-bipyridyl)ruthenium(II) (Ru[bipy]3(2+)) and cis-dichlorobis (2,2'-bipyrazyl)ruthenium(II...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1997.tb01906.x
更新日期:1997-04-01 00:00:00
abstract::Photodynamic therapy (PDT) is a way of treating malignant tumors and hyperproliferative diseases. It is based on the use of photosensitizer, herein the chlorophyll a (chl a), and a light of an appropriate wavelength. The interaction of the photosensitizer (PS) with the light produces reactive oxygen species (ROS), pow...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2012.01238.x
更新日期:2013-03-01 00:00:00
abstract::The incidence and prevalence of inflammatory bowel disease (IBD) are increasing worldwide. Some ecological studies show increasing incidence with increasing latitude. Ambient ultraviolet radiation varies inversely with latitude, and sun exposure of the skin is a major source of vitamin D. Vitamin D deficiency is commo...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/php.13007
更新日期:2019-01-01 00:00:00
abstract::The temperature dependence of the uptake phase of 5-aminolevulinic acid (ALA) and the following production phase of protoporphyrin IX (PpIX) in normal mouse skin was investigated. A cream containing 20% ALA was topically applied on the skin for 10 min. The amount of ALA-induced PpIX was evaluated by measuring the fluo...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::A new type of photochromic molecule that can be used for diffusion coefficient (D) measurements of various proteins in solution is described. The absorption spectrum of this molecule is changed upon photoexcitation by the trans-cis isomerization reaction. Target proteins were labeled by this photochromic molecule in t...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00315.x
更新日期:2008-07-01 00:00:00
abstract::The photochemistry of Ru(bpy)(3)+2 in the presence of amines was investigated in water by laser flash photolysis. N,N'-Dimethylaniline and p-phenylenediamine quench the luminescent metal to ligand charge transfer (MLCT) excited state of the complex by an electron transfer reaction that produces the semireduced form Ru...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2005-05-31-RA-558
更新日期:2006-01-01 00:00:00
abstract::To determine the impact of delivery vehicles in photosensitizing efficacy of HPPH, a hydrophobic photosensitizer was dissolved in various formulations: 1% Tween 80/5% dextrose, Pluronic P-123 and Pluronic F-127 in 0.5%, 1% and 2% phosphate buffer solutions (PBS). HPPH was also conjugated to Pluronic F-127, and the res...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13183
更新日期:2020-05-01 00:00:00
abstract::We examined the effects of daily exposure to UVB on growth, reproduction and histological characteristics of Daphnia magna over two generations at 20, 22, 25 and 30°C. Animals were exposed to 16 h of UVA and photosynthetically active radiation daily. Treated animals received 6 h of UVB during the light phase. Parental...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2012.01197.x
更新日期:2013-01-01 00:00:00
abstract::The dependence of autofluorescence properties on the metabolic and functional engagement and on the transformation condition was studied on single cells. Normal Galliera rat fibroblasts at low subculture passage (cell strain), at high subculture passage (stabilized cell line), and transformed cell line derived from a ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(1999)069<0364:dofapo>2.3.co;2
更新日期:1999-03-01 00:00:00