Abstract:
:A new type of photochromic molecule that can be used for diffusion coefficient (D) measurements of various proteins in solution is described. The absorption spectrum of this molecule is changed upon photoexcitation by the trans-cis isomerization reaction. Target proteins were labeled by this photochromic molecule in the dark and the translational motion of the proteins was detected by the transient grating (TG) method. The TG signal was simple enough to determine D accurately and was stable even for long-time irradiation by the laser light. The TG method using this probe molecule improves many drawbacks of the other techniques.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Eitoku T,Terazima Mdoi
10.1111/j.1751-1097.2008.00315.xsubject
Has Abstractpub_date
2008-07-01 00:00:00pages
869-73issue
4eissn
0031-8655issn
1751-1097pii
PHP315journal_volume
84pub_type
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