Abstract：
:A solution of iodouracil (0.2 mg/mL) and KI (0.1 M) in 0.23 M borate buffer (pH 7) forms triiodide upon exposure to UVB. In the presence of thyodene, the blue starch-iodine complex is formed, the intensity of which is proportional to the amount of UVB exposure. Studies were conducted using either a sunlamp or solar irradiation to generate the triiodide endpoint. Samples (1 mL each) were placed in a plastic 24 well sample holder for exposure, and the intensity of the light at each well was controlled by placing a template over the holder with apertures of various sizes (0.4-1.5 cm) to vary the amount of light incident on each sample. The endpoint was measured using a spectrometer to monitor the absorbance increase at 400 nm of each sample or a densitometer to monitor the optical density of a photograph of the resulting solutions. The selectivity for UVB was demonstrated by irradiating through a UVB cutoff filter that reduced the sunlamp yield by 98.5% and the solar radiation yield by 94%. The correlation between the observed absorbance changes at 400 nm and the amount of absorbed energy was shown to be approximately the same for both types of radiation and the resulting quantum yield calculated to be approximately 1%, consistent with the value obtained previously for 5-iododeoxyuridine using monochromatic radiation. It is proposed that this system be incorporated into a personal dosimeter for monitoring solar UVB exposures, where the degree of exposure can be estimated either by visual inspection or, for more quantitative analysis, by UV spectroscopy.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Rahn R,Lee MAsubject
Has Abstractpub_date
1998-08-01 00:00:00pages
173-8issue
2eissn
0031-8655issn
1751-1097journal_volume
68pub_type
杂志文章abstract：:The role of water molecules in spectral tuning of proteins has been left largely unexplored. This topic is important because changing hydrogen bond patterns during the activation process may lead to spectral shifts which can be of diagnostic value for the underlying structures. Arguments put forward in this article ar...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2008.00499.x
更新日期：2009-03-01 00:00:00
abstract：:Herein, we present a study of the pharmacokinetics and biodistribution of a butadiyne-linked conjugated porphyrin dimer (Oxdime) designed to have high near-infrared (NIR) 2-photon absorption cross-section for photodynamic therapy (PDT). Changes in biodistribution over time were monitored in mice carrying B16-F10 melan...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2012.01194.x
更新日期：2012-11-01 00:00:00
abstract：:Ultraviolet-A irradiation of a suprofen (2-[4-2(2-thenoyl) phenyl]propionic acid) (SPF) buffered solution (pH 7.4) in the presence of supercoiled pBR322 DNA leads to single strand breaks with the formation of an open circular form and subsequent linearization of the plasmid. On the basis of agarose gel electrophoresis...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb05252.x
更新日期：1995-07-01 00:00:00
abstract：:Direct irradiation of estrone aryl and methyl sulfonates in different organic solvents under nitrogen atmosphere was investigated under steady-state conditions. The estrone derivatives reacted efficiently through the photo-Fries rearrangement reaction involving [1;3]-sulfonyl migration providing the ortho-sulfonyl est...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13272
更新日期：2021-01-01 00:00:00
abstract：:Tetracycline molecules offer several sites for peroxidative metabolism of the type known to lead to oxygen consumption and electronic excitation. Accordingly, when tetracycline and chlortetracycline were exposed to horseradish peroxidase in the presence of hydrogen peroxide, oxygen was taken up and light emission was ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb09588.x
更新日期：1993-09-01 00:00:00
abstract：:Antisense oligodeoxynucleotides directed against a 24-mer RNA derived from the long terminal repeat (LTR) region of HIV were linked to proto- and methylpyrroporphyrin and their zinc derivatives. The oligonucleotide-porphyrin conjugates were tested for their ability to induce photodamage on the target RNA. Upon hybridi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05110.x
更新日期：1994-10-01 00:00:00
abstract：:The pyrimidine nucleobases contained in DNA undergo a variety of photoinduced reactions in which two moieties become joined to form a product (e.g. formation of cyclobutane dimers and [6-4] adducts). Herein, we describe a new type of photoconjugation reaction that has been shown to occur for 5-methylcytosine (5-MeC), ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.01070.x
更新日期：2012-03-01 00:00:00
abstract：:Molecular beacons (MB) are becoming more common as sequence-selective detectors of nucleic acids. Although they can easily detect single-base mismatches, they have never been used to directly detect DNA or RNA damage. To measure the degree of ultraviolet (UV) light damage in oligonucleotides, we report a novel MB appr...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-09-02-RA-301
更新日期：2005-03-01 00:00:00
abstract：:The photophysical properties of Piroxicam, a nonsteroidal anti-inflammatory drug (NSAID), were investigated at different pHext values in reversed micelles of Aerosol-OT (AOT) in iso-octane, using both steady-state and picosecond time-resolved fluorescence spectroscopy. In contrast with the very complex data obtained i...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:The significance of human cutaneous pigmentation lies in its protective role against sun-induced DNA damage and photocarcinogenesis. Fair skin and red hair are characterized by a low eumelanin to pheomelanin ratio, and have been associated with increased risk of skin cancer. Cutaneous pigmentation is a complex genetic...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2011.00970.x
更新日期：2011-09-01 00:00:00
abstract：:The fluoroquinolone (FQ) antibiotics photosensitize human skin to solar UV radiation and are reported to photosensitize tumor formation in mouse skin. As tumor initiation will not occur without genotoxic insult, we examined the potential of ciprofloxacin, lomefloxacin, fleroxacin, BAYy3118 (a recently developed monofl...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1997.tb03160.x
更新日期：1997-09-01 00:00:00
abstract：:Singlet oxygen reacts preferentially with three amino acids in proteins, His, Trp and Met. In order to study the specific molecular events that result from such oxidations, calf alpha-crystallin was photooxidized in the presence of uroporphyrin and the reactions were investigated by high performance liquid chromatogra...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb02330.x
更新日期：1993-03-01 00:00:00
abstract：:A novel In(3+)-doped TiO(2) and TiO(2)/In(2)S(3) nanocomposites for photocatalytic degradation of environmental pollutants and stoichiometric degradation of warfare agents were prepared by a homogeneous hydrolysis with urea and thioacetamide, respectively. The prepared samples series TiInTAA were annealed at 600°C. Th...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.01052.x
更新日期：2012-03-01 00:00:00
abstract：:The energy and oscillator strength of electronic transitions of chlorophyll (Chl)-amino acid complexes were calculated by using molecular orbital methods. The energies varied widely with coordinated amino acids and the difference between the maximum and minimum energy was about 830 cm-1. This energy difference was com...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2001)073<0245:aomoct>2.0.co;2
更新日期：2001-03-01 00:00:00
abstract：:The UVA and UVB components of sunlight can produce three classes of bipyrimidine DNA photolesions [cyclobutane pyrimidine dimers (CPDs), pyrimidine (6-4) pyrimidone photoproducts (6-4PPs) and related Dewar valence isomers (DewarPPs)]. The UVA/UVB ratio of sunlight is high in winter and low in summer in the Northern He...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13361
更新日期：2020-12-03 00:00:00
abstract：:The XPA gene-deficient mouse, an animal model of xeroderma pigmentosum (XP), develops enhanced photobiologic reactions including acute inflammation, immunosuppression and skin carcinogenesis, because of the defect in the excision repair of ultraviolet-induced DNA lesions. The results strongly suggest that nuclear DNA ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1562/2006-03-02-IR-829
更新日期：2007-01-01 00:00:00
abstract：:The immunological consequences of exposure to UVA (320-400 nm) radiation are unclear. This study describes the relationship between the generation of epidermal cis-urocanic acid and the ability to respond to a contact-sensitizing agent, in hairless mice exposed to different UV radiation sources, which incorporate succ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05103.x
更新日期：1994-09-01 00:00:00
abstract：:Plant responses to light spectral quality can be exploited to deliver a range of agronomically desirable end points in protected crops. This can be achieved using plastics with specific spectral properties as crop covers. We have studied the responses of a range of crops to plastics that have either (a) increased tran...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-12-06-RA-392
更新日期：2005-09-01 00:00:00
abstract：:Polyenic systems are involved in light perception of numerous living organisms. Although a π-conjugated backbone is a common feature of all polyenes, their photophysics may vary. We provide a comparative quantum mechanical study of low-lying S1 and S2 excited states in short (3-5 double bonds) symmetric all-trans line...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12833
更新日期：2017-11-01 00:00:00
abstract：:Although ultraviolet-B (UV-B) radiation is potentially harmful, it is an important component of terrestrial radiation to which plants have been exposed since invading land. Since then, plants have evolved mechanisms to avoid and repair UV radiation damage; therefore, it is not surprising that photomorphogenic response...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1562/0031-8655(2003)078<0529:hduprc>2.0.co;2
更新日期：2003-12-01 00:00:00
abstract：:The incidence of skin cancer is increasing rapidly, particularly in the Caucasian population. Epidemiological and experimental studies demonstrated that ultraviolet radiation (UVR) is the primary cause for the increasing incidence of skin cancer. It is well known that UV irradiation induces DNA damage. If the damage i...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：:The pH-insensitive beetle luciferases cloned from Rhagophthalmidae, Phengodidae, and Elateridae exhibit great potential application as reporter assays for monitoring gene expression. At present, however, only one luciferase has been reported from the enigmatic and predominantly Asian distributed luminous family Rhagop...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13172
更新日期：2020-01-01 00:00:00
abstract：:Ring-fused retinal analogs were designed to examine the hula-twist mode of the photoisomerization of the 9-cis retinylidene chromophore. Two 9-cis retinal analogs, the C11-C13 five-membered ring-fused and the C12-C14 five-membered ring-fused retinal derivatives, formed the pigments with opsin. The C11-C13 ring-fused a...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)076<0606:coosot>2.0.co;2
更新日期：2002-12-01 00:00:00
abstract：:High temperature can promote cyanobacterial blooms, whereas ultraviolet radiation (UVR) can potentially depress cyanobacterial growth by damaging their photosynthetic apparatus. Although the damaging effect of UVR has been well documented, reports on the interactive effects of UV radiation exposure and warming on cyan...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13377
更新日期：2021-01-04 00:00:00
abstract：:Within this study, the erythemal ultraviolet (UV) exposure received by different parts of the body during four different activities is determined. Optoelectronic devices were used to measure the erythemal UV exposure at 10 different positions of the body. The measuring devices were fixed on the forehead, on the should...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12085
更新日期：2013-07-01 00:00:00
abstract：:It has previously been demonstrated that chronic low-dose solar-simulated UV radiation could induce both local and systemic immunosuppression as well as tolerance to a topically applied hapten. In this study, we have used a chronic low-dose UV-irradiation protocol to investigate the effects of UVA on the skin immune s...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1996.tb01863.x
更新日期：1996-12-01 00:00:00
abstract：:Limited trypsinization of large pea (Pisum sativum cv. Alaska) phytochrome and subsequent size-exclusion chromatography (SEC) in 0.1 M Na phosphate, pH 7.8, yielded a high-molecular-mass aggregate of tryptic fragments of phytochrome. Further SEC in 0.1 M Tris-HCl, pH 7.5, plus various concentrations of NaSCN, indicate...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1989.tb04136.x
更新日期：1989-07-01 00:00:00
abstract：:The phototransduction cascade is perhaps the best understood model system for G protein-coupled receptor (GPCR) signaling. Phototransduction links the absorption of a single photon of light to a decrease in cytosolic cGMP. Depletion of the cGMP pool induces closure of cGMP-gated cation channels resulting in the hyperp...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2008.00516.x
更新日期：2009-03-01 00:00:00
abstract：:The photodegradation of the herbicide clomazone in the presence of S(2)O(8) (2-) or of humic substances of different origin was investigated. A value of (9.4 +/- 0.4) x 10(8) m(-1) s(-1) was measured for the bimolecular rate constant for the reaction of sulfate radicals with clomazone in flash-photolysis experiments. ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2008.00467.x
更新日期：2009-05-01 00:00:00
abstract：:It is generally assumed that a central metal is essential for the efficiency of phthalocyanines in photodynamic therapy (PDT) of cancer. Contrary to the set opinion, the results of the present study indicate that the metal-free sulfonated phthalocyanines (H2PcSn, where n is the number of sulfonate groups per molecule)...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)075<0527:cwmine>2.0.co;2
更新日期：2002-05-01 00:00:00
