Abstract：
:The fluoroquinolone (FQ) antibiotics photosensitize human skin to solar UV radiation and are reported to photosensitize tumor formation in mouse skin. As tumor initiation will not occur without genotoxic insult, we examined the potential of ciprofloxacin, lomefloxacin, fleroxacin, BAYy3118 (a recently developed monofluorinated quinolone) and a nalidixic acid to photosensitize DNA damage in V79 hamster fibroblasts in vitro. Cells were exposed to 37.5 kJ/m2 UVA (320-400 nm; glass filtered Sylvania psoralen + UVA (PUVA) tubes; calibrated Waldmann radiometer) at 4 degrees C in the presence of FQ and immediately afterwards embedded in agarose, lysed and placed in an electrophoretic field at pH 12. Under these denaturing conditions, the presence of DNA single-strand breaks (SSB), alkali-labile sites (ALS) and double-strand breaks (DSB) can be visualized as DNA migrating away from the nucleus (characteristic "comet" appearance) after staining with a specific fluorochrome. At FQ concentrations that induced minimal loss of cell viability (neutral red uptake assay) the compounds tested induced comets with a rank order of BAYy3118 > norfloxacin > ciprofloxacin > lomefloxacin > fleroxacin > nalidixic acid. If cells were incubated after treatment for 1 h at 37 degrees C, the comet score decreased, suggesting efficient removal of SSB/ALS/DSB. Addition of the DNA polymerase(alpha) inhibitor, aphidicolin, to cells treated with either ciprofloxacin alone or ciprofloxacin + UVA resulted in an accumulation of SSB due to the endo/exonuclease steps of excision repair. We have demonstrated that the FQ are photogenotoxic in mammalian cells but the FQ-photosensitized SSB are efficiently repaired. Preliminary evidence that ciprofloxacin photosensitizes the formation of DNA lesions warranting excision repair may indicate production of more mutagenic lesions.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Reavy HJ,Traynor NJ,Gibbs NKdoi
10.1111/j.1751-1097.1997.tb03160.xsubject
Has Abstractpub_date
1997-09-01 00:00:00pages
368-73issue
3eissn
0031-8655issn
1751-1097journal_volume
66pub_type
杂志文章abstract：:The multifunctional polypeptide cyclosporin synthetase (CySyn) remains one of the most complex nonribosomal peptide synthetase described. In this study we used a highly specific photoaffinity labeling procedure with the natural cofactor S-adenosyl-L-methionine (AdoMet), 14C-isotopically labeled at the Sdelta methyl gr...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2003)077<0129:plotnm>2.0.co;2
更新日期：2003-02-01 00:00:00
abstract：:The infrared spectrum of monomeric unsubstituted coumarin (C9H6O2; 2H-1-benzopyran-2-one), isolated in solid argon at 10 K is presented and assigned. The UV-induced (lambda>200 nm) unimolecular photochemistry of the matrix-isolated compound was studied experimentally. Three main photoreactions were observed: (a) decar...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00152.x
更新日期：2007-09-01 00:00:00
abstract：:It has been proposed that photodegradation of folates may be the reason for the pigmentation of races living under high fluence rates of ultraviolet radiation. The photodegradation of folic acid (FA) induced by ultraviolet-A (UV-A) radiation, in solution and in the presence of human serum albumin (HSA), was studied wi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2005-11-23-RA-739
更新日期：2006-05-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1992.tb04226.x
更新日期：1992-02-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-03-17-RA-117
更新日期：2004-09-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12317
更新日期：2014-11-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2001)074<0712:iosnri>2.0.co;2
更新日期：2001-11-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)072<0731:hsawtp>2.0.co;2
更新日期：2000-12-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12419
更新日期：2015-05-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb02928.x
更新日期：1993-04-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 临床试验,杂志文章
doi：10.1111/j.1751-1097.1996.tb03130.x
更新日期：1996-10-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2011.00970.x
更新日期：2011-09-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb02382.x
更新日期：1995-09-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.12723
更新日期：2017-03-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12169
更新日期：2014-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13245
更新日期：2020-05-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)076<0417:hnsodd>2.0.co;2
更新日期：2002-10-01 00:00:00
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doi：10.1111/php.13301
更新日期：2020-11-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb05277.x
更新日期：1995-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb09881.x
更新日期：1995-06-01 00:00:00
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doi：10.1562/2003-11-19-ra.1
更新日期：2004-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2018-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：2015-09-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1989.tb04136.x
更新日期：1989-07-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb04914.x
更新日期：1993-07-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12107
更新日期：2013-11-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1989.tb09197.x
更新日期：1989-04-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2001)074<0760:qosmoo>2.0.co;2
更新日期：2001-12-01 00:00:00
