Luciferin-Regenerating Enzyme Crystal Structure Is Solved but its Function Is Still Unclear.


:Contribution of luciferin-regenerating enzyme (LRE) for in vitro recycling of D-luciferin has been reported. According to crystal structure of LRE, it is a beta-propeller protein which is a type of all β-protein architecture. In this overview, reinvestigation of the luciferase-based LRE assays and its function is reported. Until now, sequence of LRE genes from four different species of firefly has been reported. In spite of previous reports, T-LRE (from Lampyris turkestanicus) was cloned and expressed in Escherichia coli as well as Pichia pastoris in a nonsoluble form as inclusion body. According to recent investigations, bioluminescent signal of soluble T-LRE-luciferase-coupled assay increased and then reached an equilibrium state in the presence of D-cysteine. In addition, the results revealed that both D- and L-cysteine in the absence of T-LRE caused a significant increase in bioluminescence intensity of luciferase over a long time. Based on activity measurements and spectroscopic results, D-cysteine increased the activity of luciferase due to its redox potential and induction of conformational changes in structure and kinetics properties. In conclusion, in spite of previous reports on the effect of LRE (at least T-LRE) on luciferase activity, most of the increase in luciferase activity is caused by direct effect of D-cysteine on structure and activity of firefly luciferase. Moreover, bioinformatics analysis cannot support the presence of LRE in peroxisome of photocytes in firefly lanterns.


Photochem Photobiol


Hosseinkhani S,Emamgholi Zadeh E,Sahebazzamani F,Ataei F,Hemmati R




Has Abstract


2017-03-01 00:00:00












  • Development of an in vitro system for the analysis of ultraviolet radiation-induced suppression of natural killer cell activity.

    abstract::Previous studies have shown that natural killer (NK) cell activity was suppressed in volunteer subjects exposed to ultraviolet radiation (UVR) from solarium lamps. The present studies were carried out to determine the spectrum of UVR responsible for suppression of NK activity and to develop in vitro methods to analyze...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Hersey P,Magrath H,Wilkinson F

    更新日期:1993-02-01 00:00:00

  • Activities of the bimodal fluorescent protein produced by Photobacterium phosphoreum strain bmFP in the luciferase reaction in vitro.

    abstract::The activity of the bimodal fluorescent protein (bmFP) (lambda max, 488 and 517 nm) in the in vitro luciferase reaction has been studied. The bmFP that is produced by Photobacterium phosphoreum strain bmFP is a dimer of two homologous subunits binding four riboflavin 5'-phosphate (FMN)-myristate chromophores. The addi...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Karatani H,Konaka T

    更新日期:2000-02-01 00:00:00

  • Sub-lethal photodynamic damage to ARPE-19 cells transiently inhibits their phagocytic activity.

    abstract::Efficient phagocytosis of photoreceptor outer segments (POS) membranes by retinal pigment epithelium (RPE) plays a key role in biological renewal of these highly peroxidizable structures. Here, we tested whether photodynamic treatment, mediated by merocyanine 540 (MC 540), rose Bengal or a zinc-substituted chlorophyll...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Olchawa M,Szewczyk G,Zareba M,Piłat A,Bzowska M,Mikołajczyk T,Sarna T

    更新日期:2010-07-01 00:00:00

  • Light reflected from red mulch to ripening strawberries affects aroma, sugar and organic acid concentrations.

    abstract::Strawberry (Fragaria ananassa) fruit size and flavor are important to both growers and consumers. Plastic mulches are frequently used in raised-bed culture to conserve water, control weeds with less herbicides, keep fruit clean and produce ripe berries earlier in the season. The most commonly used plastic mulch color ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Kasperbauer MJ,Loughrin JH,Wang SY

    更新日期:2001-07-01 00:00:00

  • Photosensitization reactions of fluoroquinolones and their biological consequences.

    abstract::This review focuses on damage photosensitized by the fluoroquinolone (FQ) antibacterial drugs. Different models are employed to study biosubstrate photodamage mediated by FQs (organisms, cells, isolated biomolecules and super molecules). Being that the effect of environment (polarity of the medium, ions, pH, binding w...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审


    authors: de Guidi G,Bracchitta G,Catalfo A

    更新日期:2011-11-01 00:00:00

  • Molecular beacon probes of photodamage in thymine and uracil oligonucleotides.

    abstract::Molecular beacons (MB) are becoming more common as sequence-selective detectors of nucleic acids. Although they can easily detect single-base mismatches, they have never been used to directly detect DNA or RNA damage. To measure the degree of ultraviolet (UV) light damage in oligonucleotides, we report a novel MB appr...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Yarasi S,McConachie C,Loppnow GR

    更新日期:2005-03-01 00:00:00

  • Wavelength dependence of DNA incision by a human ultraviolet endonuclease.

    abstract::The wavelength dependence of an ultraviolet irradiation of the DNA substrate for a human endonuclease was determined. Sites of DNA incision for all UVB and UVC wavelengths examined were at cytosines which were neither cyclobutane pyrimidine dimers nor 6-4'-(pyrimidin-2-one)pyrimidines. The optimal wavelengths for form...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Gallagher PE,Weiss RB,Brent TP,Duker NJ

    更新日期:1989-03-01 00:00:00

  • Targeted photochemical modification of HIV-derived oligoribonucleotides by antisense oligodeoxynucleotides linked to porphyrins.

    abstract::Antisense oligodeoxynucleotides directed against a 24-mer RNA derived from the long terminal repeat (LTR) region of HIV were linked to proto- and methylpyrroporphyrin and their zinc derivatives. The oligonucleotide-porphyrin conjugates were tested for their ability to induce photodamage on the target RNA. Upon hybridi...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


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    更新日期:1994-10-01 00:00:00

  • Chronology of the apoptotic events induced in the K562 cell line by photodynamic treatment with hematoporphyrin and monoglucosylporphyrin.

    abstract::Photodynamic treatment of promyelocytic K562 cells in the presence of a monoglucosylporphyrin or hematoporphyrin leads to a sequence of events recognized as hallmarks of apoptosis: a drop in mitochondrial potential, concurrent with a drop in ATP level and a decrease in cell respiration, translocation of phosphatidylse...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


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    更新日期:1999-01-01 00:00:00

  • Theoretical Study of Fluorescence Spectra Utilizing the pKa Values of Acids in Their Excited States.

    abstract::Assignment of the fluorescence spectrum of firefly luciferin in aqueous solutions was achieved by utilizing not only emission energies but also theoretical absorption spectra and relative concentrations as estimated by pKa values. Calculated Gibbs free energies were utilized to estimate pKa values. These pKa values we...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Hiyama M,Akiyama H,Yamada K,Koga N

    更新日期:2014-01-01 00:00:00

  • Effect of vitamin E on cytotoxicity, DNA single strand breaks, chromosomal aberrations, and mutation in Chinese hamster V-79 cells exposed to ultraviolet-B light.

    abstract::The effect of pretreatment with vitamin E on cytotoxicity, DNA single strand breaks, and chromosomal aberrations as well as on mutation induced by ultraviolet-B light (UV-B) was investigated in Chinese hamster V-79 cells. Cellular pretreatment with non-toxic levels of 25 microM alpha-tocopherol succinate (vitamin E) f...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Sugiyama M,Tsuzuki K,Matsumoto K,Ogura R

    更新日期:1992-07-01 00:00:00

  • Ring opening photoreactions of 5-bromouracil and 5-bromo-2'-deoxyuridine with selected alkylamines.

    abstract::Several studies in the literature indicate that histones (lysine rich proteins found associated with DNA in eukaryotic chromatin), as well as poly-L-lysine, can be photocross-linked by ultraviolet (UV) light to DNA in which 5-bromo-2'-deoxyuridine has been substituted for thymidine. To gain some insight into the possi...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Shetlar MD,Rose RB,Hom K,Shaw AA

    更新日期:1991-05-01 00:00:00

  • Photochemical linkage of antibodies to silicon chips.

    abstract::Antibodies and antigen binding fragments thereof were photochemically immobilized on surface-modified silicon chips of 5 x 5 mm size. Silicon surface-grafted diazirines and benzophenones formed covalent bonds with the immunoreagents on light activation. Photolithographic immobilization of monoclonal antibodies in aque...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Sundarababu G,Gao H,Sigrist H

    更新日期:1995-06-01 00:00:00

  • Photoactivation of hypericin generates singlet oxygen in mitochondria and inhibits succinoxidase.

    abstract::Photosensitized inhibition of mitochondrial succinoxidase by hypericin was measured in vitro and found to be drug-dose, light-dose, and wavelength dependent. Singlet oxygen generation, monitored using the singlet oxygen trap tetramethylethylene, and oxygen consumption in isolated mitochondria sensitized by hypericin w...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Thomas C,MacGill RS,Miller GC,Pardini RS

    更新日期:1992-01-01 00:00:00

  • Photochemical inactivation of Pseudomonas aeruginosa.

    abstract::Adaptability to a broad range of environments together with relatively high resistance to antibiotics and to disinfectants makes Pseudomonas aeruginosa a concern in hospitals and in public health. We investigated whether UVA-mediated photochemical inactivation of P. aeruginosa could be accomplished with high efficienc...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Sagripanti JL,Grote G,Niederwöhrmeier B,Hülseweh B,Marschall HJ

    更新日期:2012-01-01 00:00:00

  • Contact transcleral ciliary body photodynamic therapy with verteporfin in pigmented rabbits: effect of repeated treatments.

    abstract::We studied the effect on the intraocular pressure (IOP) and the ciliary body (CB) morphology after four consecutive contact transcleral photodynamic treatments of the ciliary body (CB-PDT) with verteporfin in pigmented rabbits. Twenty-two pigmented rabbits underwent CB-PDT (study group), performed once (six rabbits) o...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Charisis SK,Naoumidi II,Ginis HS,Detorakis ET,Tsilimbaris MK

    更新日期:2010-01-01 00:00:00

  • Calculation of singlet oxygen dose using explicit and implicit dose metrics during benzoporphyrin derivative monoacid ring A (BPD-MA)-PDT in vitro and correlation with MLL cell survival.

    abstract::Photodynamic therapy (PDT) oxygen consumption, clonogenic cell survival, fluorescence photobleaching and photoproduct formation were investigated during benzoporphyrin derivative monoacid (BPD-MA)-PDT of MAT-LyLu cells in vitro. Cells were incubated with BPD-MA concentrations of 0.1, 0.5 or 2.5 μg mL(-1) for 2 h and t...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Weston MA,Patterson MS

    更新日期:2011-09-01 00:00:00

  • The Different Resistance of Two Astragalus Plants to UV-B Stress is Tightly Associated with the Organ-specific Isoflavone Metabolism.

    abstract::In this work, the changes in isoflavone levels and the expression of genes involved in their biosynthesis were studied in two Astragalus by UPLC-MS and real-time PCR after 10 days of UV-B treatment (λmax  = 313 nm, 804 J m-2 ). Isoflavones were significantly induced by UV-B irradiation. The influence might be activate...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Liu Y,Liu J,Wang Y,Abozeid A,Tian DM,Zhang XN,Tang ZH

    更新日期:2018-01-01 00:00:00

  • Cloning of the Orange Light-Producing Luciferase from Photinus scintillans-A New Proposal on how Bioluminescence Color is Determined.

    abstract::Unlike the enchanting yellow-green flashes of light produced on warm summer evenings by Photinus pyralis, the most common firefly species in North America, the orange lights of Photinus scintillans are infrequently observed. These Photinus species, and likely all bioluminescent beetles, use the same substrates beetle ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Branchini BR,Southworth TL,Fontaine DM,Murtiashaw MH,McGurk A,Talukder MH,Qureshi R,Yetil D,Sundlov JA,Gulick AM

    更新日期:2017-03-01 00:00:00

  • Revisiting the Mechanism of Hydrolysis of Betanin.

    abstract::Betanin (betanidin 5-O-β-D-glucoside) is a water-soluble plant pigment used as a color additive in food, drugs and cosmetic products. Despite its sensitivity to light and heat, betanin maintains appreciable tinctorial strength in low acidic and neutral conditions, where the color of other plant pigments, such as antho...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Esteves LC,Pinheiro AC,Pioli RM,Penna TC,Baader WJ,Correra TC,Bastos EL

    更新日期:2018-09-01 00:00:00

  • Pheophorbide a-induced photo-oxidation of cytochrome c: implication for photodynamic therapy.

    abstract::Pheophorbide a-induced photo-oxidation, in vitro, of cytochrome c oxidase and cytochrome c results in irreversible modifications to both protein components. Photo-oxidation of cytochrome c, as exhibited by change in its heme oxidation state, displays exponential kinetics and is detected with a lag period. Both the pho...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Chernomorsky S,Wong C,Poretz RD

    更新日期:1992-02-01 00:00:00

  • Psoralen Derivatives with Enhanced Potency.

    abstract::Psoralen is a furocoumarin natural product that intercalates within DNA and forms covalent adducts when activated by ultraviolet radiation. It is well known that this property contributes to psoralen's clinical efficacy in several disease contexts, which include vitiligo, psoriasis, graft-versus-host disease and cutan...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Buhimschi AD,Gooden DM,Jing H,Fels DR,Hansen KS,Beyer WF Jr,Dewhirst MW,Walder H,Gasparro FP

    更新日期:2020-09-01 00:00:00

  • Do melanoidins induced by topical 9% dihydroxyacetone sunless tanning spray inhibit vitamin d production? A pilot study.

    abstract::We report here preliminary pilot study data of the effect of sunless tanning spray with 9% [Correction added after online publication (August 24th, 2009): The concentration of Dihydroxyacetone used in the study was 9% and not 3% as previously stated] dihydroxyacetone (DHA) on 25-hydroxyvitamin D [25(OH)D] serum levels...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Armas LA,Fusaro RM,Sayre RM,Huerter CJ,Heaney RP

    更新日期:2009-09-01 00:00:00

  • Quantum efficiencies of the reversible photoreaction of octopus rhodopsin.

    abstract::The classic method of photometric curves for photosensitivity determination has been extended to the case of photoreversible reactions and applied to the octopus rhodopsin --> acid metarhodopsin photoreaction. In such cases, measurements at one irradiation wavelength yield the sum of the photosensitivities of the forw...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Dixon SF,Cooper A

    更新日期:1987-07-01 00:00:00

  • Computational analysis of the oxygen addition at the C4a site of reduced flavin in the bacterial luciferase bioluminescence reaction.

    abstract::The energetic characteristics of selected reaction steps in the bacterial luciferase-catalyzed luminescence reaction were examined by computation using the MNDO-PM3 method. Specifically, a three-step model was proposed to account for the reaction between oxygen and reduced riboflavin 5'-phosphate (1,5H2-FMN) to genera...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Wada N,Sugimoto T,Watanabe H,Tu SC

    更新日期:1999-07-01 00:00:00

  • Multispectral imaging autofluorescence microscopy for the analysis of lymph-node tissues.

    abstract::Although histochemical and immunohistochemical methods are the standard procedures in diagnosis of lymphoproliferative disorders, useful improvements in evidencing histopathologic manifestations can be obtained with the introduction of tissue autofluorescence analyses. We used microspectrofluorometry and a Multispectr...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Rigacci L,Alterini R,Bernabei PA,Ferrini PR,Agati G,Fusi F,Monici M

    更新日期:2000-06-01 00:00:00

  • Resonance Raman and surface-enhanced resonance Raman spectra of LH2 antenna complex from Rhodobacter sphaeroides and Ectothiorhodospira sp. excited in the Qx and Qy transitions.

    abstract::Well-resolved vibrational spectra of LH2 complex isolated from two photosynthetic bacteria, Rhodobacter sphaeroides and Ectothiorhodospira sp., were obtained using surface-enhanced resonance Raman scattering (SERRS) exciting into the Qx and the Qy transitions of bacteriochlorophyll a. High-quality SERRS spectra in the...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Chumanov G,Picorel R,Ortiz de Zarate I,Cotton TM,Seibert M

    更新日期:2000-05-01 00:00:00

  • Cyclooxygenase-2 expression in murine and human nonmelanoma skin cancers: implications for therapeutic approaches.

    abstract::Inflammatory stimuli result in the production of cutaneous eicosanoids, which are known to contribute to the process of tumor promotion. Cyclooxygenase (COX), the rate-limiting enzyme for the production of prostaglandins (PG) from arachidonic acid, exists in at least two isoforms, COX-1 and COX-2. COX-1 is constitutiv...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: An KP,Athar M,Tang X,Katiyar SK,Russo J,Beech J,Aszterbaum M,Kopelovich L,Epstein EH Jr,Mukhtar H,Bickers DR

    更新日期:2002-07-01 00:00:00

  • Cryptococcus gattii: in vitro susceptibility to photodynamic inactivation.

    abstract::Cryptococus gattii is an emergent primary human pathogen that causes meningismus, papilledema, high intracranial pressure and focal involvement of the central nervous system in immunocompetent hosts. Prolonged antifungal therapy is the conventional treatment, but it is highly toxic, selects for resistant strains, cont...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Soares BM,Alves OA,Ferreira MV,Amorim JC,Sousa GR,Silveira Lde B,Prates RA,Avila TV,Baltazar Lde M,de Souza Dda G,Santos DA,Modolo LV,Cisalpino PS,Pinotti M

    更新日期:2011-03-01 00:00:00

  • A Photoprotective Effect by Cation-π-Interaction? Quenching of Singlet Oxygen by an Indole Cation-π Model System.

    abstract::We investigated the effect of the cation-π interaction on the susceptibility of a tryptophan model system toward interaction with singlet oxygen, that is, type II photooxidation. The model system consists of two indole units linked to a lariat crown ether to measure the total rate of removal of singlet oxygen by the i...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Arevalo GE,Cagan DA,Monsour CG,Garcia AC,McCurdy A,Selke M

    更新日期:2020-11-01 00:00:00