Abstract:
:An ongoing study in our laboratories is to examine the relationship of DNA repair defects to human cancer. Our underlying hypothesis has been that human tumors may arise that lack interesting DNA repair pathways if these pathways are important in preventing cancer. In this study, we found that the UV-irradiated adenoviruses showed hypersensitivity when assayed on monolayers of certain human colon tumor cell lines, including three that are reported to have defects in long patch DNA mismatch repair genes and one with no reported defect in mismatch repair. The survival curves showed two components. The first sensitive component was characteristic of 77-95% of the infections depending upon the cell line and the experiment and had an average slope indicating 4.8-fold hypersensitivity to UV. The average of the second-component slopes indicated that the remainder of the infections was accompanied by near-normal repair. Although the value of the first component indicated that the colon tumor lines supported the growth of UV-damaged adenoviruses poorly, the cell lines themselves showed the same post-UV colony-forming ability as did normal human fibroblasts, and their ability to support the growth of N-methyl-N'-nitro-N-nitrosoguanidine-damaged adenoviruses was normal, i.e. it parallelled their ability to repair O6-methylguanine in vitro. We previously observed two-component survival curves when assaying UV-irradiated adenovirus on monolayers of all of seven strains of fibroblasts from Cockayne's syndrome patients. By contrast, single-component curves have been obtained using 21 strains of normal human fibroblasts and seven other tumor lines. We interpret the two-component survival curves in terms of the defective transcription-coupled repair of UV-induced DNA damage that is characteristic both of Cockayne's and certain colon tumor cell lines. In addition, four mismatch repair-deficient colon tumor lines were resistant to killing by elevated levels of dG.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Day RS 3rd,Rasouli-Nia A,Meservy J,Lari SU,Dobler K,Tsunoda S,Miyakoshi J,Takebe H,Murray Dsubject
Has Abstractpub_date
1999-08-01 00:00:00pages
217-27issue
2eissn
0031-8655issn
1751-1097journal_volume
70pub_type
杂志文章abstract::Reactive nitrogen species, produced during the process of inflammation induced by various factors including UV radiation, modify amino acids in crucial proteins. It is assumed that skin tissue is more likely to be modified, as it is located at the outer layer of a body that is exposed to UV radiation on a daily basis....
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
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doi:10.1111/j.1751-1097.1994.tb05000.x
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
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pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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doi:
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:1990-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
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pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:1999-06-01 00:00:00