Theoretical Study of Fluorescence Spectra Utilizing the pKa Values of Acids in Their Excited States.


:Assignment of the fluorescence spectrum of firefly luciferin in aqueous solutions was achieved by utilizing not only emission energies but also theoretical absorption spectra and relative concentrations as estimated by pKa values. Calculated Gibbs free energies were utilized to estimate pKa values. These pKa values were then corrected by employing the experimental results. It was previously thought that the main peak near 550 nm observed in the experimental fluorescence spectra at all pH values corresponds to emission from the first excited state of the luciferin dianion [Ando et al. (2010) Jpn. J. Appl. Phys. 49, 117002-117008]. However, we found that the peak near 550 nm at low pH corresponds to emission from the first excited state of the phenolate monoanion of luciferin. Furthermore, we found that the causes of the red fluorescence at pH 1-2 are not only the emission from phenol monoanion but also the emission from the protonated species at nitrogen atom in the thiazoline ring of dianion.


Photochem Photobiol


Hiyama M,Akiyama H,Yamada K,Koga N




Has Abstract


2014-01-01 00:00:00












  • Ultraviolet radiation exposure, vitamin D, and cancer.

    abstract::This paper reviews briefly the evidence for an association between various measures of UV radiation (UVR) exposure and the development of cancer. Issues such as data quality, study design, measurement variation, comparability of studies, and quantification of UV exposure in relation to skin cancer are discussed. A ran...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审


    authors: Berwick M,Kesler D

    更新日期:2005-11-01 00:00:00

  • Photochemical linkage of antibodies to silicon chips.

    abstract::Antibodies and antigen binding fragments thereof were photochemically immobilized on surface-modified silicon chips of 5 x 5 mm size. Silicon surface-grafted diazirines and benzophenones formed covalent bonds with the immunoreagents on light activation. Photolithographic immobilization of monoclonal antibodies in aque...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Sundarababu G,Gao H,Sigrist H

    更新日期:1995-06-01 00:00:00

  • Effects of supplementary ultraviolet-B irradiance on maize yield and qualities: a field experiment.

    abstract::Stratospheric ozone depletion has caused an increase in the amount of ultraviolet-B (UV-B) radiation reaching the earth's surface. Numerous investigations have demonstrated that the effect of UV-B enhancements on plants includes reduction in grain yield, alteration in species competition, susceptibility to disease and...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Gao W,Zheng Y,Slusser JR,Heisler GM,Grant RH,Xu J,He D

    更新日期:2004-07-01 00:00:00

  • Effect of oxygen on photoautotrophic and heterotrophic growth of Chlamydomonas reinhardtii in an anoxic atmosphere.

    abstract::Photoautotrophic growth of Chlamydomonas reinhardtii was shown to be independent of the presence of atmospheric oxygen. Under constant light and photoautotrophic conditions, C. reinhardtii grew equally well in either air or 367 PPM CO2-in-He. During 12-h light-dark cycles, the cells in air grew substantially faster th...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Graves DA,Spradlin GM,Greenbaum E

    更新日期:1990-09-01 00:00:00

  • UVB-induced inflammatory cytokine release, DNA damage and apoptosis of human oral compared with skin tissue equivalents.

    abstract::People can get oral cancers from UV (290-400 nm) exposures. Besides high outdoor UV exposures, high indoor UV exposures to oral tissues can occur when consumers use UV-emitting tanning devices to either tan or whiten their teeth. We compared the carcinogenic risks of skin to oral tissue cells after UVB (290-320 nm) ex...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Breger J,Baeva L,Agrawal A,Shindell E,Godar DE

    更新日期:2013-05-01 00:00:00

  • Time-resolved singlet oxygen phosphorescence measurements from photosensitized experiments in single cells: effects of oxygen diffusion and oxygen concentration.

    abstract::Time-resolved singlet oxygen, O(2)(a(1)Delta(g)), phosphorescence experiments have been performed in single cells upon pulsed laser irradiation of a photosensitizer incorporated into the cell. Data recorded as a function of the partial pressure of ambient oxygen to which the cell is exposed reflect apparent values for...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Hatz S,Poulsen L,Ogilby PR

    更新日期:2008-09-01 00:00:00

  • Subcellular distribution of hypericin in human cancer cells.

    abstract::Confocal laser microspectrofluorometric measurements on human T47D mammary tumor cells have been performed to assess the intracellular distribution of hypericin within the various cell compartments: cytoplasmic membrane, cytoplasm and nucleus. Confocal fluorescence measurements obtained from microvolumes (approximatel...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Miskovsky P,Sureau F,Chinsky L,Turpin PY

    更新日期:1995-09-01 00:00:00

  • On the Formation of Thymine Photodimers in Thymine Single Strands and Calf Thymus DNA.

    abstract::Solar light leads to thymine dimers that are mutagenic and primary cause of skin cancer. Here, we report absorption and synchrotron radiation circular dichroism (CD) spectra of Tn single strands with different number n of bases (n = 2-7, 10, 11) recorded after various 254 nm irradiation times. From a principal compone...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Baggesen LM,Hoffmann SV,Nielsen SB

    更新日期:2014-01-01 00:00:00

  • A comparison of protoporphyrin IX and protoporphyrin IX dimethyl ester as a photosensitizer in poorly differentiated human nasopharyngeal carcinoma cells.

    abstract::Protoporphyrin IX dimethyl ester (PME), a dimethyl esterification of protoporphyrin IX (PpIX), exhibits higher intracellular uptake into NPC/CNE2 cells, a poorly differentiated human nasopharyngeal carcinoma, than does PpIX. Phototoxicity studies reveal PME to be a more potent photosensitizer than is PpIX, at the earl...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Yee KK,Soo KC,Bay BH,Olivo M

    更新日期:2002-12-01 00:00:00

  • Effect of Light Environment on Growth and Phenylpropanoids of Yarrow (Achillea collina cv. SPAK) Grown in the Alps.

    abstract::A 2-year field study on the effect of different light environments, obtained by using cladding materials (polyethylene films and shade net) able to cut off specific regions of the photosynthetically active radiation and ultraviolet wavebands, on the growth and phenylpropanoids content of Achillea collina grown in the ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Giorgi A,Manzo A,Vagge I,Panseri S

    更新日期:2014-01-01 00:00:00

  • Synthesis, structure and photophysical properties of ferrocenyl or mixed sandwich cobaltocenyl ester linked meso-tetratolylporphyrin dyads.

    abstract::We report here the design and synthesis of porphyrin-metallocene dyads consisting of a metallocene [either ferrocene or mixed sandwich η(5)-[C5H4(COOH)]Co(η(4)-C4Ph4) connected via an ester linkage at meso phenyl position of either free-base or zinc porphyrin. All these dyad systems were characterized by various spect...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Gokulnath S,Achary BS,Kumar CK,Trivedi R,Sridhar B,Giribabu L

    更新日期:2015-01-01 00:00:00

  • Excited state processes in 1-deazariboflavin studied by ultrafast fluorescence kinetics.

    abstract::The photochemical reactions of the 1-deaza derivative of riboflavin (RF) have been studied by sub-ps time-resolved fluorescence spectroscopy. A potential effect of the solvent polarity and hydrogen bonding capability on the excited state (ES) dynamics was tested by measuring 1-deaza RF in water and, after acetylation ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Slavov C,Mansurova M,Holzwarth AR,Gärtner W

    更新日期:2010-01-01 00:00:00

  • H-NMR studies of duplex DNA decamer containing a uracil cyclobutane dimer: implications regarding the high UV mutagenecity of CC photolesions.

    abstract::To determine the origin of the UV-specific CC to TT tandem mutation at the CC site, we made a duplex DNA decamer containing a uracil cis-syn cyclobutane dimer (CBD) as the deaminated model of a cytosine dimer. Two-dimensional 1H-NMR spectroscopy studies were performed on this sequence where two adenines (Ade) were opp...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Lee HM,Kim JK

    更新日期:2002-10-01 00:00:00

  • Effects of short- and long-term ultraviolet B irradiation on the immune system of the common carp (Cyprinus carpio).

    abstract::Carp (Cyprinus carpio) were repeatedly exposed to 0, 60, 120 and 240 mJ/cm2 ultraviolet B (UVB) radiation three times in 1 week (short-term exposure) or 12 times in 4 weeks (long-term exposure). The effect of UVB on the functioning of the carp immune system was studied on day 2 after the final irradiation. After short...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Markkula SE,Salo HM,Immonen AK,Jokinen EI

    更新日期:2005-05-01 00:00:00

  • 5-Aminolaevulinic acid methyl ester transport on amino acid carriers in a human colon adenocarcinoma cell line.

    abstract::The transport mechanisms of 5-aminolevulinic acid methyl ester (5-ALA-ME) have been studied in a human adenocarcinoma cell line (WiDr) by means of 14[C]-labeled 5-ALA-ME. The transport was found to be partly Na+ dependent, while the extracellular Cl- concentration did not affect the uptake. The transport of 5-ALA-ME i...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Gederaas OA,Holroyd A,Brown SB,Vernon D,Moan J,Berg K

    更新日期:2001-02-01 00:00:00

  • In vitro photodynamic effects of lysyl chlorin p6: cell survival, localization and ultrastructural changes.

    abstract::The in vitro cell survival, localization and ultrastructural changes following irradiation were examined in 9L glioma cells sensitized with a new photosensitizer, lysyl chlorin p6 (LCP). In clonogenic assays, LCP was 10-100-fold more phototoxic than photofrin II on a microgram/mL basis. Lysyl chlorin p6 uptake was blo...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Leach MW,Higgins RJ,Autry SA,Boggan JE,Lee SJ,Smith KM

    更新日期:1993-11-01 00:00:00

  • Protein diffusion probed by the transient grating method with a new type of photochromic molecule.

    abstract::A new type of photochromic molecule that can be used for diffusion coefficient (D) measurements of various proteins in solution is described. The absorption spectrum of this molecule is changed upon photoexcitation by the trans-cis isomerization reaction. Target proteins were labeled by this photochromic molecule in t...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Eitoku T,Terazima M

    更新日期:2008-07-01 00:00:00

  • Mutants of Ca2+ -regulated Photoprotein Obelin for Site-specific Conjugation.

    abstract::Color variants of Ca2+ -regulated photoprotein obelin were shown to be an important tool for dual-analyte binding assay. To provide site-directed conjugation with biospecific molecules, several obelin color mutants carrying unique cysteine residues were obtained and characterized for their novel properties. A pair of ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审


    authors: Krasitskaya VV,Burakova LP,Komarova AA,Bashmakova EE,Frank LA

    更新日期:2017-03-01 00:00:00

  • Ultraviolet germicidal irradiation: future directions for air disinfection and building applications.

    abstract::Ultraviolet germicidal irradiation (UVGI) for air disinfection applications has relied on low-pressure mercury vapor lamps for decades. New design requirements have generated the need for alternatives in some uses. This study describes the current state of UVGI technology and describes future directions for technology...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审


    authors: Miller SL,Linnes J,Luongo J

    更新日期:2013-07-01 00:00:00

  • Photophysics and photochemistry of z-chlorprothixene in acetonitrile.

    abstract::Chlorprothixene (CPTX, Taractan) is a low potency antipsychotic mainly used for the treatment of psychotic disorders (e.g. schizophrenia) and acute mania occurring as part of bipolar disorders. As in the case of other numerous drugs used in the treatment of psychiatric disorders, CPTX presents geometric isomerism. The...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Piñero LE,García C,Lhiaubet-Vallet V,Oyola R,Miranda MA

    更新日期:2009-07-01 00:00:00

  • Oxygen dependence of hypericin-induced phototoxicity to EMT6 mouse mammary carcinoma cells.

    abstract::The photodynamic effect of hypericin on EMT6 mouse mammary carcinoma cells was investigated in vitro under aerobic and hypoxic conditions. Under aerobic conditions, hypericin-induced photocytotoxicity was dose dependent within a 1-50 microM range. Under hypoxic conditions, cells were resistant to hypericin-induced pho...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Thomas C,Pardini RS

    更新日期:1992-06-01 00:00:00

  • Furocoumarin-induced epidermal melanogenesis does not protect against skin photocarcinogenesis in hairless mice.

    abstract::Topical 6,4,4'-trimethylangelicin (TMA) plus UVA was used to induce intense epidermal pigmentation in inbred HRA.HRII-c/+/Skh hairless pigmented mice over a 13 day period. Subsequent UVB/UVA exposure was used to assess the photoprotective properties of this tan using skin tumors as an endpoint. Comparisons were always...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Kipp C,Lewis EJ,Young AR

    更新日期:1998-01-01 00:00:00

  • Ultraviolet B but not A radiation activates suppressor B cells in draining lymph nodes.

    abstract::Immunosuppressive doses of solar-simulated UV radiation activate lymph node B cells that can suppress primary immunity by inhibiting the function of dendritic cells. The aim of this study was to determine the waveband responsible for activation of these suppressor B cells. We exposed C57BL/6 mice to various doses of e...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Byrne SN,Ahmed J,Halliday GM

    更新日期:2005-11-01 00:00:00

  • Steric hindrance between chromophore substituents as the driving force of rhodopsin photoisomerization: 10-methyl-13-demethyl retinal containing rhodopsin.

    abstract::A visual chromophore analogue, 10-methyl-13-demethyl (dm) retinal, was synthesized and reconstituted with bleached bovine rhodopsin to form a visual pigment derivative with absorbance maximum at 505 nm. The investigations with this new compound were stimulated from recent results using 13-dm retinal as a chromophore t...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Koch D,Gärtner W

    更新日期:1997-01-01 00:00:00

  • Iodouracil as a personal dosimeter for solar UVB.

    abstract::A solution of iodouracil (0.2 mg/mL) and KI (0.1 M) in 0.23 M borate buffer (pH 7) forms triiodide upon exposure to UVB. In the presence of thyodene, the blue starch-iodine complex is formed, the intensity of which is proportional to the amount of UVB exposure. Studies were conducted using either a sunlamp or solar ir...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Rahn R,Lee MA

    更新日期:1998-08-01 00:00:00

  • The influence of water on the photophysical and photochemical properties of Piroxicam in AOT/iso-octane/water reversed micelles

    abstract::The photophysical properties of Piroxicam, a nonsteroidal anti-inflammatory drug (NSAID), were investigated at different pHext values in reversed micelles of Aerosol-OT (AOT) in iso-octane, using both steady-state and picosecond time-resolved fluorescence spectroscopy. In contrast with the very complex data obtained i...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Andrade SM,Costa SM,Pansu R

    更新日期:2000-04-01 00:00:00

  • Photodecomposition of vitamin A and photobiological implications for the skin.

    abstract::Vitamin A (retinol), an essential human nutrient, plays an important role in cellular differentiation, regulation of epidermal cell growth and normal cell maintenance. In addition to these physiological roles, vitamin A has a rich photochemistry. Photoisomerization of vitamin A, involved in signal transduction for vis...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审


    authors: Fu PP,Xia Q,Yin JJ,Cherng SH,Yan J,Mei N,Chen T,Boudreau MD,Howard PC,Wamer WG

    更新日期:2007-03-01 00:00:00

  • Comparative study of photodynamic properties of 13,15-N-cycloimide derivatives of chlorin p6.

    abstract::Comparative study of 13,15-[N-(2-hydroxyethyl)]cycloimide chlorin p6 (2), 13,15-(N-acetoxy)cycloimide chlorin p6 (3), 13,15-(N-hydroxy)cycloimide chlorin p6 methyl ester (4) and 13,15-(N-methoxy)cycloimide chlorin p6 methyl ester (5) together with the previously investigated 13,15-[N-(3-hydroxypropyl)]cycloimide chlor...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Feofanov A,Sharonov G,Grichine A,Karmakova T,Pljutinskaya A,Lebedeva V,Ruziyev R,Yakubovskaya R,Mironov A,Refregier M,Maurizot JC,Vigny P

    更新日期:2004-02-01 00:00:00

  • Spectral shift of ultraweak photon emission from sweet potato during a defense response.

    abstract::Consecutive spectral analyses of ultraweak photon emission from sweet potato showing a defense response were conducted to observe the process of physiological transition. The spectrum showed a drastic transition from 2 to 10 h after inoculation with Fusarium oxysporum, during which the emission intensity increased slo...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Iyozumi H,Kato K,Makino T

    更新日期:2002-03-01 00:00:00

  • A comparison of fluorescence methods used in the pharmacokinetic studies of Zn(II)phthalocyanine in mice.

    abstract::The pharmacokinetics of Zn phthalocyanine (ZnPc) encapsulated in dipalmitoyl-phosphatidylcholine (DPPC) liposomes, injected intravenously in Skh:HR-1 nude mice, was monitored by two in vitro techniques and one in vivo technique, all based on fluorescence spectroscopy. The in vitro methods involve either fluorescence m...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Biolo R,Jori G,Kennedy JC,Nadeau P,Pottier R,Reddi E,Weagle G

    更新日期:1991-01-01 00:00:00