Molecular beacon probes of photodamage in thymine and uracil oligonucleotides.

Abstract:

:Molecular beacons (MB) are becoming more common as sequence-selective detectors of nucleic acids. Although they can easily detect single-base mismatches, they have never been used to directly detect DNA or RNA damage. To measure the degree of ultraviolet (UV) light damage in oligonucleotides, we report a novel MB approach for general detection of photoproducts in UV-irradiated rU17 and dT17 oligonucleotides. With monochromatic UV light irradiation at ca 280 nm under anoxic conditions, the oligonucleotide absorption decays with a single-exponential time constant of 123+/-1 min for rU17 and with double-exponential time constants of 78+/-0.5 min (99%) and 180+/-5 min (0.05%) for dT17 oligonucleotides. Under the same conditions, the MB fluorescence decays more quickly, with single-exponential time constants of 19+/-2 and 26+/-3 min for rU17 and dT17, respectively. Similar kinetics were observed with broadband UV light irradiation of oligonucleotides. The differences in the UV damage kinetics of dT17 and rU17 and their detection by absorption and fluorescence techniques will be discussed in the context of differential instabilities introduced in the nucleic acid-MB duplex by the different photoproducts formed.

journal_name

Photochem Photobiol

authors

Yarasi S,McConachie C,Loppnow GR

doi

10.1562/2004-09-02-RA-301

subject

Has Abstract

pub_date

2005-03-01 00:00:00

pages

467-73

issue

2

eissn

0031-8655

issn

1751-1097

pii

2004-09-02-RA-301

journal_volume

81

pub_type

杂志文章
  • Optical properties of human melanocytic nevi in vivo.

    abstract::We present an in vivo study of the optical properties of melanin present in melanocytic nevi of human subjects with Fitzpatrick skin type III (Caucasian descent) using optical spectroscopy. We show that the melanin absorption spectrum exhibits an exponential dependence on wavelength with a decay constant which follows...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2008.00436.x

    authors: Zonios G,Dimou A

    更新日期:2009-01-01 00:00:00

  • The use of solar radiation by the photosynthetic bacterium, Rhodopseudomonas palustris: model simulation of conditions found in a shallow pond or a flatbed reactor.

    abstract::Photosynthetic bacteria are attractive for biotechnology because they produce no oxygen and so H2 -production is not inhibited by oxygen as occurs in oxygenic photoorganisms. Rhodopseudomonas palustris and Afifella marina containing BChl a can use irradiances from violet near-UV (VNUV) to orange (350-650 nm) light and...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12124

    authors: Ritchie RJ

    更新日期:2013-09-01 00:00:00

  • Porphyrin-cellulose nanocrystals: a photobactericidal material that exhibits broad spectrum antimicrobial activity.

    abstract::Towards our overall objectives of developing potent antimicrobial materials to combat the escalating threat to human health posed by the transmission of surface-adhering pathogenic bacteria, we have investigated the photobactericidal activity of cellulose nanocrystals that have been modified with a porphyrin-derived p...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2012.01117.x

    authors: Carpenter BL,Feese E,Sadeghifar H,Argyropoulos DS,Ghiladi RA

    更新日期:2012-05-01 00:00:00

  • 5-Aminolaevulinic acid methyl ester transport on amino acid carriers in a human colon adenocarcinoma cell line.

    abstract::The transport mechanisms of 5-aminolevulinic acid methyl ester (5-ALA-ME) have been studied in a human adenocarcinoma cell line (WiDr) by means of 14[C]-labeled 5-ALA-ME. The transport was found to be partly Na+ dependent, while the extracellular Cl- concentration did not affect the uptake. The transport of 5-ALA-ME i...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2001)073<0164:aameto>2.0.co;2

    authors: Gederaas OA,Holroyd A,Brown SB,Vernon D,Moan J,Berg K

    更新日期:2001-02-01 00:00:00

  • A Comparison of Solar Ultraviolet Radiation Exposure in Urban Canyons in Venice, Italy and Johannesburg, South Africa.

    abstract::Urban environments can have high-risk spaces that can provide excess personal sun exposure, such as urban or street canyons, and the spaces between buildings, among others. In these urban spaces, sun exposure can be high or low depending on several factors. Polysulphone film (PSF) was used to assess possible daily sol...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13291

    authors: Wright CY,du Preez DJ,Martincigh BS,Allen MW,Millar DA,Wernecke B,Blesic S

    更新日期:2020-09-01 00:00:00

  • Electron transfer quenching of the rose bengal triplet state.

    abstract::The potential for electron transfer quenching of rose bengal triplet (3RB2-) to compete with energy transfer quenching by oxygen was evaluated. Rate constants for oxidative and reductive quenching were measured in buffered aqueous solution, acetonitrile and in small unilamellar liposomes using laser flash photolysis. ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1997.tb03133.x

    authors: Lambert CR,Kochevar IE

    更新日期:1997-07-01 00:00:00

  • Photochemical syn-anti isomerization reactions in N2-hydroxyisocytosines--an experimental matrix isolation and theoretical study.

    abstract::N2-hydroxyisocytosine and 1-methyl-N2-hydroxyisocytosine were studied using a matrix isolation technique combined with infrared absorption spectroscopy. For N2-hydroxyisocytosine isolated in an Ar matrix (at 10 K), two imino-oxo isomers, one with the hydroxyimino =N-OH group directed toward the N1-H group (the form ca...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2003)077<0243:psairi>2.0.co;2

    authors: Lapinski L,Nowak MJ,Kwiatkowski JS,Leszczynski J

    更新日期:2003-03-01 00:00:00

  • FTIR spectroscopic and theoretical study of the photochemistry of matrix-isolated coumarin.

    abstract::The infrared spectrum of monomeric unsubstituted coumarin (C9H6O2; 2H-1-benzopyran-2-one), isolated in solid argon at 10 K is presented and assigned. The UV-induced (lambda>200 nm) unimolecular photochemistry of the matrix-isolated compound was studied experimentally. Three main photoreactions were observed: (a) decar...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2007.00152.x

    authors: Kuş N,Breda S,Reva I,Tasal E,Ogretir C,Fausto R

    更新日期:2007-09-01 00:00:00

  • Spectroscopic studies of cutaneous photosensitizing agents--XIV. The spin trapping of free radicals formed during the photolysis of halogenated salicylanilide antibacterial agents.

    abstract::Several antibacterial halogenated salicylanilides, including 3,3',4',5-tetrachlorosalicylanilide (TCSA) and 3,4',5-tribromosalicylanilide (TBSA) are known to cause photoallergy. We have carried out photochemical and spin trapping studies to determine whether free radicals may be involved in the photoallergic response....

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1989.tb04162.x

    authors: Chignell CF,Sik RH

    更新日期:1989-09-01 00:00:00

  • Phototaxis of Haloarcula marismortui revealed through a novel microbial motion analysis algorithm.

    abstract::Haloarcula marismortui has been described to be nonmotile prior to the recent identification of flagellar filaments, suggesting the motile nature of H. marismortui. Here we observed the locomotion of freshly cultured H. marismortui cells and tracked the swimming trajectories via ImageJ. Trajectories of H. marismortui ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2010.00768.x

    authors: Lin YC,Fu HY,Yang CS

    更新日期:2010-09-01 00:00:00

  • Solar UV exposure of seafarers along subtropical and tropical shipping routes.

    abstract::Seafarers working on decks of vessels at low latitudes are exposed to extremely high solar UV radiation. Their risk of developing skin cancer may be enhanced. Solar erythemal UV irradiance and exposure were measured for the first time on merchant vessels going along typical international routes at low latitudes. The m...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12144

    authors: Feister U,Meyer G,Kirst U

    更新日期:2013-11-01 00:00:00

  • Photointeraction of benzophenone triplet with lysozyme.

    abstract::The quenching of the benzophenone triplet by lysozyme and its constituent amino acids in aqueous solutions have been studied. Native lysozyme quenches the benzophenone triplet with a high rate constant, 4 x 10(9) M-1 s-1. The quenching process takes place with production of significant amounts of free ketyl radicals, ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1989.tb08424.x

    authors: Encinas MV,Lissi EA,Vasquez M,Olea AF,Silva E

    更新日期:1989-05-01 00:00:00

  • Multidimensional reaction coordinate for the excited-state H-atom transfer in perylene quinones: importance of the 7-membered ring in hypocrellins A and B.

    abstract::The excited-state intramolecular H-atom transfer reactions of hypocrellins B and A are compared by using time-resolved absorption and fluorescence upconversion techniques. The hypocrellin B photophysics are well described by a simple model involving one ground-state species and excited-state forward and reverse H-atom...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Datta A,Smirnov AV,Wen J,Chumanov G,Petrich JW

    更新日期:2000-02-01 00:00:00

  • New Perspectives on an Old Issue: A Comparative MS-CASPT2 and OM2-MRCI Study of Polyenes and Protonated Schiff Bases.

    abstract::Polyenic systems are involved in light perception of numerous living organisms. Although a π-conjugated backbone is a common feature of all polyenes, their photophysics may vary. We provide a comparative quantum mechanical study of low-lying S1 and S2 excited states in short (3-5 double bonds) symmetric all-trans line...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12833

    authors: Dokukina I,Marian CM,Weingart O

    更新日期:2017-11-01 00:00:00

  • The effects of low density lipoproteins on uptake of Photofrin II.

    abstract::The cellular uptake of Photofrin II (PII) was studied using fluorescence imaging and chemical extraction. The influence of serum and low density lipoproteins (LDL) was examined under a variety of experimental conditions employing cultured human cells of different origins as well as a subcutaneously SMT-F tumor implant...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1990.tb01702.x

    authors: Korbelik M,Hung J,Lam S,Palcic B

    更新日期:1990-02-01 00:00:00

  • A Sharp Jump in Photocatalytic Activity of Elemental Sulfur for Dye Degradation in Alkaline Solution.

    abstract::Elemental sulfur is a low-cost and abundant substance as one of the largest by-products of the oil industry which was widely used in many industrial activities. Cyclo-octasulfur (S8 ) is one of the sulfur allotropes that is a very stable substance in standard conditions. In this study, we report a low-cost and fast me...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13301

    authors: Peyman VA,Azizian S

    更新日期:2020-11-01 00:00:00

  • Relative induction of cyclobutane dimers and cytosine photohydrates in DNA irradiated in vitro and in vivo with ultraviolet-C and ultraviolet-B light.

    abstract::SV40 DNA was irradiated in vitro and in vivo with UV-C (240-280 nm) and UV-B (280-320 nm) light, and damaged sites sensitive to digestion with Escherichia coli endonuclease III (endo III) and bacteriophage T4 endonuclease V (endo V) were quantified. The frequency of endo III-sensitive sites (primarily cytosine photohy...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1991.tb02084.x

    authors: Mitchell DL,Jen J,Cleaver JE

    更新日期:1991-11-01 00:00:00

  • Comparison of UV-absorbing nets in pepper crops: spectral properties, effects on plants and pest control.

    abstract::In horticultural crops, the use of screens to protect plants is the usual strategy in the Mediterranean area. Screen manufacturers offer a range of netting that vary in their UV-absorbing properties. We compared the photoeffects of seven different screens. Sweet pepper trials were conducted at the Gilat Research Cente...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2009.00657.x

    authors: Legarrea S,Karnieli A,Fereres A,Weintraub PG

    更新日期:2010-03-01 00:00:00

  • Time-resolved and steady-state fluorescence spectroscopy of eumelanin and indolic polymers.

    abstract::Eumelanin plays a variety of important physiological roles in human skin. However, its structure and fundamental properties still remain poorly understood. Although the absorbance of eumelanin is broad and reveals little about its structure, a variety of techniques have revealed the presence of a disordered array of c...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2007.00186.x

    authors: Nighswander-Rempel SP,Mahadevan IB,Rubinsztein-Dunlop H,Meredith P

    更新日期:2007-11-01 00:00:00

  • Role of nuclear factor-kappa B in colon cancer cell apoptosis mediated by aminopyropheophorbide photosensitization.

    abstract::Aminopyropheophorbide (APP) is a second generation of photosensitizer for photodynamic therapy (PDT). We demonstrated that APP strongly absorbed red light and, after being taken up by colon cancer cells (HCT-116 cells), was localized in cytoplasmic and internal membranes but not in mitochondria. The APP-mediated photo...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Matroule JY,Hellin AC,Morlière P,Fabiano AS,Santus R,Merville MP,Piette J

    更新日期:1999-10-01 00:00:00

  • Low-light-induced violaxanthin de-epoxidation in shortly preheated leaves: uncoupling from Delta pH-dependent nonphotochemical quenching.

    abstract::Plants protect themselves against excessive light by the induction of Delta pH-dependent nonphotochemical quenching (qE) that is associated with de-epoxidation of violaxanthin (V) to zeaxanthin (Z) in thylakoid membranes. In this work, we report that low light (12 micromol photons m(-2) s(-1)) is sufficient for a mark...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2009.00699.x

    authors: Ilík P,Kotabová E,Spundová M,Novák O,Kana R,Strzałka K

    更新日期:2010-05-01 00:00:00

  • Baicalin Protects Keratinocytes from Toll-like Receptor-4 Mediated DNA Damage and Inflammation Following Ultraviolet Irradiation.

    abstract::UVB radiation contributes to both direct and indirect damage to the skin including the generation of free radicals and reactive oxygen species (ROS), inflammatory responses, immunosuppression and gene mutations, which can ultimately lead to photocarcinogenesis. A plant-derived flavonoid, baicalin, has been shown to ha...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12505

    authors: Min W,Ahmad I,Chang ME,Burns EM,Qian Q,Yusuf N

    更新日期:2015-11-01 00:00:00

  • Computational analysis of the oxygen addition at the C4a site of reduced flavin in the bacterial luciferase bioluminescence reaction.

    abstract::The energetic characteristics of selected reaction steps in the bacterial luciferase-catalyzed luminescence reaction were examined by computation using the MNDO-PM3 method. Specifically, a three-step model was proposed to account for the reaction between oxygen and reduced riboflavin 5'-phosphate (1,5H2-FMN) to genera...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Wada N,Sugimoto T,Watanabe H,Tu SC

    更新日期:1999-07-01 00:00:00

  • Generation of free radicals by emodic acid and its [D-Lys6]GnRH-conjugate.

    abstract::In an attempt to develop an efficient chemotherapeutic agent targeted at malignant cells that express receptors to gonadotropin releasing hormone (GnRH) we coupled [D-Lys6]GnRH covalently to an emodin derivative, i.e. emodic acid (Emo) to yield [D-Lys6(Emo)]GnRH. Emodin is a naturally occurring anthraquinone which is ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2001)074<0226:gofrbe>2.0.co;2

    authors: Rahimipour S,Bilkis I,Péron V,Gescheidt G,Barbosa F,Mazur Y,Koch Y,Weiner L,Fridkin M

    更新日期:2001-08-01 00:00:00

  • Cancer Cell-targeted and Activatable Photoimmunotherapy Spares T Cells in a 3D Coculture Model.

    abstract::Photodynamic therapy (PDT) is an established therapeutic modality that uses nonionizing near-infrared light to activate photocytotoxicity of endogenous or exogenous photosensitizers (PSs). An ongoing avenue of cancer research involves leveraging PDT to stimulate antitumor immune responses; however, these effects appea...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13153

    authors: Kercher EM,Nath S,Rizvi I,Spring BQ

    更新日期:2020-03-01 00:00:00

  • Substituent effects on the photophysical properties of pterin derivatives in acidic and alkaline aqueous solutions.

    abstract::Pterins are heterocyclic compounds with important biological functions, and most of them may exist in two acid-base forms in the pH range between 3 and 13 in aqueous solution. In this work, the photophysical properties of acid and basic forms of six compounds of the pterin family (6-hydroxymethylpterin [HPT], 6-methyl...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2005-05-10-RA-522

    authors: Cabrerizo FM,Petroselli G,Lorente C,Capparelli AL,Thomas AH,Braun AM,Oliveros E

    更新日期:2005-09-01 00:00:00

  • Resonance Raman and surface-enhanced resonance Raman spectra of LH2 antenna complex from Rhodobacter sphaeroides and Ectothiorhodospira sp. excited in the Qx and Qy transitions.

    abstract::Well-resolved vibrational spectra of LH2 complex isolated from two photosynthetic bacteria, Rhodobacter sphaeroides and Ectothiorhodospira sp., were obtained using surface-enhanced resonance Raman scattering (SERRS) exciting into the Qx and the Qy transitions of bacteriochlorophyll a. High-quality SERRS spectra in the...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2000)071<0589:rraser>2.0.co;2

    authors: Chumanov G,Picorel R,Ortiz de Zarate I,Cotton TM,Seibert M

    更新日期:2000-05-01 00:00:00

  • Fusion-fission transport of probes and quenchers in microdomains of an amphiphilic ionene polyelectrolyte.

    abstract::In aqueous solution, amphiphilic ionenes such as the [3,22]-ionene spontaneously adopt globular conformations and form microdomains that are highly micelle-like, i.e. are capable of solubilizing organic molecules, binding and exchanging counterions and accelerating or inhibiting the rates of bimolecular reactions. Tim...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2006-09-09-RA-1036

    authors: Tcacenco CM,Quina FH

    更新日期:2007-05-01 00:00:00

  • Transient and stationary spectroscopy of cytochrome c: ultrafast internal conversion controls photoreduction.

    abstract::Photoreduction of cytochrome c (Cyt c) has been reinvestigated using femtosecond-to-nanosecond transient absorption and stationary spectroscopy. Femtosecond spectra of oxidized Cyt c, recorded in the probe range 270-1000 nm, demonstrate similar evolution upon 266 or 403 nm excitation: an ultrafast 0.3 ps internal conv...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2007.00219.x

    authors: Löwenich D,Kleinermanns K,Karunakaran V,Kovalenko SA

    更新日期:2008-01-01 00:00:00

  • Relationship between skin color and sun exposure history: a statistical classification approach.

    abstract::In this study our aim was to determine the biophysical values of constitutive skin color in Caucasians and to define the correlation between skin color and phototype assessed according to the Fitzpatrick method. Constitutive skin color was measured on the buttock, with a Minolta CR-200 colorimeter, in a population-of ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1997.tb08569.x

    authors: Rubegni P,Cevenini G,Flori ML,Fimiani M,Stanghellini E,Molinu A,Barbini P,Andreassi L

    更新日期:1997-02-01 00:00:00