Abstract：
:This study compares the abilities of the glutathione (GSH) and thioredoxin (Trx) antioxidant systems in defending cultured human lens epithelial cells (LECs) against UVA light. Levels of GSH were depleted with either L-buthionine-(S,R)-sulfoximine (BSO) or 1-chloro-2,4-dinitrobenzene (CDNB). CDNB treatment also inhibited the activity of thioredoxin reductase (TrxR). Two levels of O2 , 3% and 20%, were employed during a 1 h exposure of the cells to 25 J cm(-2) of UVA radiation (338-400 nm wavelength, peak at 365 nm). Inhibition of TrxR activity by CDNB, combined with exposure to UVA light, produced a substantial loss of LECs and cell damage, with the effects being considerably more severe at 20% O2 compared to 3%. In contrast, depletion of GSH by BSO, combined with exposure to UVA light, produced only a slight cell loss, with no apparent morphological effects. Catalase was highly sensitive to UVA-induced inactivation, but was not essential for protection. Although UVA light presented a challenge for the lens epithelium, it was well tolerated under normal conditions. The results demonstrate an important role for TrxR activity in defending the lens epithelium against UVA light, possibly related to the ability of the Trx system to assist DNA synthesis following UVA-induced cell damage.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Padgaonkar VA,Leverenz VR,Bhat AV,Pelliccia SE,Giblin FJdoi
10.1111/php.12404subject
Has Abstractpub_date
2015-03-01 00:00:00pages
387-96issue
2eissn
0031-8655issn
1751-1097journal_volume
91pub_type
杂志文章abstract：:The XPA gene-deficient mouse, an animal model of xeroderma pigmentosum (XP), develops enhanced photobiologic reactions including acute inflammation, immunosuppression and skin carcinogenesis, because of the defect in the excision repair of ultraviolet-induced DNA lesions. The results strongly suggest that nuclear DNA ...
journal_title：Photochemistry and photobiology
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journal_title：Photochemistry and photobiology
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journal_title：Photochemistry and photobiology
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
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更新日期：2012-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：:We report the preparation of aminopropyl-functionalized magnesium phyllosilicate (Mg-organoclay) conjugated to fluorescein isothiocyanate (FITC) functionality (FITC-organoclay) by one-pot sol-gel synthesis. The physical characteristics of the Mg- and FITC-organoclays were examined by scanning electron microscopy (SEM)...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2009.00701.x
更新日期：2010-05-01 00:00:00
