Abstract：
:Cultured cells accumulate acridine orange (AO), which is a weak basic dye and a photosensitizer, in lysosomes and other acidic compartments. During exposure to blue light, AO-loaded macrophages show decreasing red granular fluorescence and increasing green diffuse fluorescence. This is hypothesized to represent peroxidative damage to lysosomal membranes resulting in an impaired proton gradient with deprotonation of the AO to its uncharged form and subsequent leakage of the dye. Further damage to the lysosomal membranes will result in release of lytic enzymes from the lysosomal compartment into the cytosol, leading to degeneration and finally cell death. The survival of AO-loaded and light-exposed macrophages is controllable by varying the exposure times to blue light. Inhibition of lysosomal proteases by E-64 results in increased cell survival after AO and blue light-mediated damage, indicating a role of proteolytic enzymes in this type of damage. Morphological analysis shows 'rounding up' with formation of retraction fibrils and pronounced plasma membrane blebbing. The formation of autophagic vacuoles is an early and pronounced event. After protease inhibition, however, all these phenomena are inhibitable to a considerable degree. We have thus directed photooxidative damage selectively to lysosomal membranes and their contents. This technique will allow further detailed studies of the role of lysosomes in degeneration-regeneration processes.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Zdolsek JM,Olsson GM,Brunk UTdoi
10.1111/j.1751-1097.1990.tb01685.xsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
67-76issue
1eissn
0031-8655issn
1751-1097journal_volume
51pub_type
杂志文章abstract：:Channel and pump rhodopsins use energy from light absorbed by a covalently bound retinal chromophore to transport ions across membranes of microbial cells. Ion transfer steps, including proton transfer, can couple to changes in protein conformational dynamics and water positions. Although general principles of how mic...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.12790
更新日期：2017-11-01 00:00:00
abstract：:A new water-soluble porphyrin derivative, 2,4-bis(1-decyloxyethyl)-deuteroporphyrinyl-6,7-bisaspart ic acid (C10-DP), and its metal complexes (Ga, I(n), Zn, Mn, Cu, Ni and Fe) were examined for their physicochemical properties (absorption, fluorescence, triplet lifetime and partition coefficient) and photocytotoxicity...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb02928.x
更新日期：1993-04-01 00:00:00
abstract：:The photobiologic properties of a chlorin-thiobarbiturate conjugate were examined. In addition to the characteristic 670 nm chlorin absorbance, the conjugate exhibited new absorbance bands at 517 and 743 nm, but these were not a part of the fluorescence emission spectra, nor was fluorescence observed at wavelengths > ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb02981.x
更新日期：1994-05-01 00:00:00
abstract：:We conducted a thermovisual comparison of mean hand surface temperature changes upon local heating with two different IR sources. Sixty-six patients with rheumatoid arthritis (47 women and 19 men; average age, 56.1 ± 8.6 years) were subjected to topical heat therapy for one hand with either the standard IR radiator (S...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.00975.x
更新日期：2011-11-01 00:00:00
abstract：:A portable fluorimeter was developed and utilized to acquire fluorescence spectra from 381 cervical sites in 95 patients at 337, 380 and 460 nm excitation immediately prior to colposcopy. A multivariate statistical algorithm was used to extract clinically useful information from tissue spectra acquired in vivo. Two fu...
journal_title：Photochemistry and photobiology
pub_type： 临床试验,杂志文章
doi：10.1111/j.1751-1097.1996.tb03130.x
更新日期：1996-10-01 00:00:00
abstract：:When Escherichia coli cells were irradiated by UVA in the presence of 6-mercaptopurine (6-MP) or 2-thiouracil (S2Ura), two kinds of repair-deficient strains of recA- and uvrA- were killed more efficiently than the parental wild-type strain having normal repair capacities. In addition, these agents with UVA exposure gr...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1997.tb01885.x
更新日期：1997-01-01 00:00:00
abstract：:Three Donor-π-Acceptor-π-Donor type styryl dyes (5a-c) with different secondary donors are synthesized and characterized to study their nonlinear and linear optical properties. The structure-property relationships of the dyes are described in the light of systematic photophysical and theoretical investigations. The ph...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13089
更新日期：2019-07-01 00:00:00
abstract：:Polyenic systems are involved in light perception of numerous living organisms. Although a π-conjugated backbone is a common feature of all polyenes, their photophysics may vary. We provide a comparative quantum mechanical study of low-lying S1 and S2 excited states in short (3-5 double bonds) symmetric all-trans line...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12833
更新日期：2017-11-01 00:00:00
abstract：:UV light leads to release of different secretory factors from irradiated cells of which some of them have been characterized. We have reported earlier that cells exposed to the supernatant medium from irradiated cells were resistant to killing by some genotoxic agents. In this study, we present our finding that demons...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2012.01105.x
更新日期：2012-05-01 00:00:00
abstract：:We performed in situ experiments during the austral summer of 1998 to quantify the mortality of the fresh-water copepod Boeckella gracilipes as a function of the UV dose. The copepods were exposed to solar radiation at the water-surface for approximately 24-34 h. Long-pass cut-off filters (Schott) were used in the exp...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)072<0314:bwfftm>2.0.co;2
更新日期：2000-09-01 00:00:00
abstract：:Sapphyrins and a series of related porphyrinoid macrocycles have been investigated as potential agents for the treatment of leishmaniasis. The effectiveness of the compounds was evaluated in vitro upon incubation with Leishmania tarentolae or L. panamensis amastigotes and promastigotes. Their effectiveness was also as...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.01034.x
更新日期：2012-01-01 00:00:00
abstract：:Use of near infrared instead of visible light would markedly improve tissue penetration, making larger tumors candidates for photochemotherapy. Because common photesensitizers exhibit virtually no absorption in this wavelength region, conditions are required where the simultaneous action of two photons is possible. He...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb05277.x
更新日期：1995-08-01 00:00:00
abstract：:The effect of charge on the cellular uptake, localization and phototoxicity of conjugates between chlorin(e6) (ce6) and poly-L-lysine was studied in vitro. These conjugates (average MW 35-55 kDa) were synthesized to have polycationic, polyanionic or neutral charges. Two human cell lines (A431 epidermoid carcinoma cell...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1997.tb01916.x
更新日期：1997-04-01 00:00:00
abstract：:Oculocutaneous albinism (OCA) is a genetically inherited autosomal recessive condition. Individuals with OCA lack melanin and therefore are susceptible to the harmful effects of solar ultraviolet radiation, including extreme sun sensitivity, photophobia and skin cancer. OCA is a grave public health issue in sub-Sahara...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.12359
更新日期：2015-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00206.x
更新日期：2008-01-01 00:00:00
abstract：:Neutral filter elution at pH 7.2 and 9.6 was used to measure the induction of DNA lesions in human P3 teratocarcinoma cells by monochromatic 254-, 270-, 313-, 334-, 365-, and 405-nm radiation and by 60 gamma rays. In this assay DNA double-strand breaks (dsb) increase the rate of elution of DNA from cell lysates on a f...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1990.tb04194.x
更新日期：1990-08-01 00:00:00
abstract：:Action spectroscopy has a long history and is of central importance to photobiological studies. Action spectra were among the first assays to point to chlorophyll as the molecule most responsible for plant growth and to DNA as the genetic material. It is useful to construct action spectra early in the investigation of...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.1991.tb02103.x
更新日期：1991-11-01 00:00:00
abstract：:The recently discovered photo-activated adenylyl cyclase (mPAC from Microcoleus chthonoplastes) is the first PAC that owes a light-, oxygen- and voltage-sensitive (LOV) domain for blue-light sensing. The photoreaction of the mPAC receptor was studied by time-resolved UV/vis and light-induced Fourier transform infrared...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12746
更新日期：2017-05-01 00:00:00
abstract：:This paper focuses on the estimation of the UV Index (UVI) for all sky conditions using a simple analytical parameterization involving three independent variables: the solar zenith angle, the total ozone column and the clearness index. Measurements of the UVI made at Badajoz and Cáceres (Southwestern Spain) from Janua...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2010.00860.x
更新日期：2011-03-01 00:00:00
abstract：:Topical 6,4,4'-trimethylangelicin (TMA) plus UVA was used to induce intense epidermal pigmentation in inbred HRA.HRII-c/+/Skh hairless pigmented mice over a 13 day period. Subsequent UVB/UVA exposure was used to assess the photoprotective properties of this tan using skin tumors as an endpoint. Comparisons were always...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:We studied the effect on the intraocular pressure (IOP) and the ciliary body (CB) morphology after four consecutive contact transcleral photodynamic treatments of the ciliary body (CB-PDT) with verteporfin in pigmented rabbits. Twenty-two pigmented rabbits underwent CB-PDT (study group), performed once (six rabbits) o...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2009.00638.x
更新日期：2010-01-01 00:00:00
abstract：:Plant responses to light spectral quality can be exploited to deliver a range of agronomically desirable end points in protected crops. This can be achieved using plastics with specific spectral properties as crop covers. We have studied the responses of a range of crops to plastics that have either (a) increased tran...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-12-06-RA-392
更新日期：2005-09-01 00:00:00
abstract：:Betanin (betanidin 5-O-β-D-glucoside) is a water-soluble plant pigment used as a color additive in food, drugs and cosmetic products. Despite its sensitivity to light and heat, betanin maintains appreciable tinctorial strength in low acidic and neutral conditions, where the color of other plant pigments, such as antho...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12897
更新日期：2018-09-01 00:00:00
abstract：:Bacteriochlorophyll (BChl) e was coassembled with BChl c in Triton X-100 micelles in aqueous solutions. The Qy absorption bands of the coaggregates were positioned between those of aggregates consisting solely of BChl c or e. The electronic absorption spectra of the coaggregates could not be reproduced by linear combi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12219
更新日期：2014-05-01 00:00:00
abstract：:Syntheses for the new photosensitizers HOSiPcOSi(CH3)2(CH2)3N(CH1)1 or 3(CH3)2, Pc 34 and Pc 25, have been developed and the order of activity of these photosensitizers and the previously reported photosensitizer Pc 4, HOSiPcOSi(CH3)2(CH2)3N(CH3)2, in the dark and with broad-band red light toward Plasmodium falciparum...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1997.tb08656.x
更新日期：1997-08-01 00:00:00
abstract：:The mutant T203V of the light driven chloride pump halorhodopsin from Halobacterium salinarum was crystallized and the X-ray structure was solved at 1.6 angstroms resolution. The T203V structure turned out to be nearly identical to the wild type protein with a root mean square deviation of 0.43 angstroms for the carbo...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2006-06-23-RA-947
更新日期：2007-03-01 00:00:00
abstract：:Cumulative and intermittent sun exposure are risk factors for skin cancer, highlighting the need to monitor exposure during childhood. There is currently very little available information concerning the accuracy of self-reported levels of sun exposure, particularly for very young children. In this study, UV radiation ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)071<0060:cbramu>2.0.co;2
更新日期：2000-01-01 00:00:00
abstract：:The photophysical properties of 1,1'-diethyl-4,4'-carbocyanine chloride (kryptocyanine) have been measured in methanol solution and for the dye bound to human serum albumin, incorporated in neutral micelles and after incubation with leukemia cells. In all cases, it is found that formation of the triplet state of the d...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1990.tb08674.x
更新日期：1990-10-01 00:00:00
abstract：:We examined the effects of daily exposure to UVB on growth, reproduction and histological characteristics of Daphnia magna over two generations at 20, 22, 25 and 30°C. Animals were exposed to 16 h of UVA and photosynthetically active radiation daily. Treated animals received 6 h of UVB during the light phase. Parental...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2012.01197.x
更新日期：2013-01-01 00:00:00
abstract：:A targeting strategy based on the selective enzyme-mediated activation of polymeric photosensitizer prodrugs (PPP) within pathological tissue has led to the development of agents with the dual ability to detect and treat cancer. Herein, a detailed study of a simple model system for these prodrugs is described. We prep...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00090.x
更新日期：2007-07-01 00:00:00
