Abstract:
:Channel and pump rhodopsins use energy from light absorbed by a covalently bound retinal chromophore to transport ions across membranes of microbial cells. Ion transfer steps, including proton transfer, can couple to changes in protein conformational dynamics and water positions. Although general principles of how microbial rhodopsins function are largely understood, key issues pertaining to reaction mechanisms remain unclear. In this review, we compare the protonation-coupled dynamics of pump and channelrhodopsins, highlighting the roles that water dynamics, protein electrostatics and protein flexibility can have in ion transport mechanisms. We discuss observations supporting important functional roles of inter- and intra-helical carboxylate/hydroxyl hydrogen-bonding motifs. As specific examples, we use the proton pump bacteriorhodopsin, the sodium pump KR2, channelrhodopsins and Anabaena sensory rhodopsin. We outline the usefulness of theoretic biophysics approaches to the study of retinal proteins, challenges in studying the hydrogen-bond dynamics of rhodopsin active sites, and implications for conformational coupling in membrane transporters.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Bondar AN,Smith JCdoi
10.1111/php.12790subject
Has Abstractpub_date
2017-11-01 00:00:00pages
1336-1344issue
6eissn
0031-8655issn
1751-1097journal_volume
93pub_type
杂志文章,评审abstract::Quantum yields of firefly bioluminescence reactions were determined for beetle luciferases from the three main families of luminous beetles emitting different bioluminescence colors. Quantum yield (QY) was significantly correlated with luminescence spectrum. The green light-emitting luciferase of the Brazilian click b...
journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:2015-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-12-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:2020-03-01 00:00:00
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journal_title:Photochemistry and photobiology
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更新日期:2020-01-01 00:00:00
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journal_title:Photochemistry and photobiology
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pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
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更新日期:2020-07-01 00:00:00
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:2000-09-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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journal_title:Photochemistry and photobiology
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更新日期:1999-06-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
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更新日期:2019-01-01 00:00:00