Abstract:
:Quantum yields of firefly bioluminescence reactions were determined for beetle luciferases from the three main families of luminous beetles emitting different bioluminescence colors. Quantum yield (QY) was significantly correlated with luminescence spectrum. The green light-emitting luciferase of the Brazilian click beetle, Pyrearinus termitilluminans, whose luminescence spectrum had the shortest peak wavelength of all the luciferases investigated, had the highest QY (0.61). Mutant analyses of active site-substituted Pyrocoelia miyako luciferases showed that, although k(cat) was decreased by the mutations, the QY was not significantly affected.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Niwa K,Ichino Y,Kumata S,Nakajima Y,Hiraishi Y,Kato D,Viviani VR,Ohmiya Ydoi
10.1111/j.1751-1097.2010.00777.xsubject
Has Abstractpub_date
2010-09-01 00:00:00pages
1046-9issue
5eissn
0031-8655issn
1751-1097pii
PHP777journal_volume
86pub_type
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