Abstract：
:The energetic characteristics of selected reaction steps in the bacterial luciferase-catalyzed luminescence reaction were examined by computation using the MNDO-PM3 method. Specifically, a three-step model was proposed to account for the reaction between oxygen and reduced riboflavin 5'-phosphate (1,5H2-FMN) to generate first the 5-hydroFMN-4a-peroxide (5H-FMN-4aOO-) and then the 5-hydro-4a-hydroperoxyFMN (5H-FMN-4aOOH) intermediates. Lysine (Lys-H+) and aspartate (Asp-) were chosen as representative catalytic residues involved in the protonation and deprotonation processes. Results show that deprotonation at the N1 site of 1,5H2-FMN by a basic amino acid residue at the luciferase active site would efficiently accelerate the reaction rate of O2 addition to form 5H-FMN-4aOO-. The most favored site of oxygen attack is at the flavin C4a. With the aid of a catalytic acid group, the 5H-FMN-4aOO- so formed tends to undergo a spontaneous protonation reaction to yield the 5H-FMN-4aOOH.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Wada N,Sugimoto T,Watanabe H,Tu SCsubject
Has Abstractpub_date
1999-07-01 00:00:00pages
116-22issue
1eissn
0031-8655issn
1751-1097journal_volume
70pub_type
杂志文章abstract：:The phototransduction cascade is perhaps the best understood model system for G protein-coupled receptor (GPCR) signaling. Phototransduction links the absorption of a single photon of light to a decrease in cytosolic cGMP. Depletion of the cGMP pool induces closure of cGMP-gated cation channels resulting in the hyperp...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2008.00516.x
更新日期：2009-03-01 00:00:00
abstract：:In this manuscript, we demonstrate the control of film thickness and surface wettability in the photochemical immobilization of poly (vinyl alcohol) (PVA) to a self-assembled monolayer (SAM) containing a phthalimide chromophore. Surface attachment is characterized by ellipsometry and contact angle measurements. The we...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12751
更新日期：2017-10-01 00:00:00
abstract：:Four chemical preservatives commonly used in ophthalmic solutions were tested for their toxic and mutagenic potential in mouse lymphoma cells with and without exposure of the cells to ultraviolet A (UVA) radiation. The preservatives tested were benzalkonium chloride (BAK), chlorhexidine, thimerosal and ethylenediamine...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1989.tb04174.x
更新日期：1989-09-01 00:00:00
abstract：:The mechanism of caspase-3-dependent apoptosis induced by photodynamic therapy (PDT) of cultured Chinese hamster V79 cells with pheophorbide a (PPa) was investigated. The PPa-PDT induced rapid apoptosis within 30 min after irradiation of cells. This apoptosis was inhibited by the 1O2 quencher N3- and caspase-3 inhibit...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
abstract：:Channel and pump rhodopsins use energy from light absorbed by a covalently bound retinal chromophore to transport ions across membranes of microbial cells. Ion transfer steps, including proton transfer, can couple to changes in protein conformational dynamics and water positions. Although general principles of how mic...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.12790
更新日期：2017-11-01 00:00:00
abstract：:Near-UV (337 nm) photoactivation of the 5-hydroxytryptophan decarboxylation reaction producing serotonin has been observed. The photoactivation effect was investigated as a function of fluence rate and fluence, and pH. Photoactivation of decarboxylase activity was found to occur at nearly neutral pH values (low activi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1989.tb09197.x
更新日期：1989-04-01 00:00:00
abstract：:To determine the origin of the UV-specific CC to TT tandem mutation at the CC site, we made a duplex DNA decamer containing a uracil cis-syn cyclobutane dimer (CBD) as the deaminated model of a cytosine dimer. Two-dimensional 1H-NMR spectroscopy studies were performed on this sequence where two adenines (Ade) were opp...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)076<0417:hnsodd>2.0.co;2
更新日期：2002-10-01 00:00:00
abstract：:Many viral infections reach clinical significance in winter, when it is cold, relative humidity is lowest and vitamin D production from solar ultraviolet-B irradiation is at its nadir. Several autoimmune diseases, such as multiple sclerosis, type 1 diabetes mellitus and asthma, are linked to viral infections. Vitamin ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2007.00266.x
更新日期：2008-03-01 00:00:00
abstract：:Direct irradiation of estrone aryl and methyl sulfonates in different organic solvents under nitrogen atmosphere was investigated under steady-state conditions. The estrone derivatives reacted efficiently through the photo-Fries rearrangement reaction involving [1;3]-sulfonyl migration providing the ortho-sulfonyl est...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13272
更新日期：2021-01-01 00:00:00
abstract：:High temperature can promote cyanobacterial blooms, whereas ultraviolet radiation (UVR) can potentially depress cyanobacterial growth by damaging their photosynthetic apparatus. Although the damaging effect of UVR has been well documented, reports on the interactive effects of UV radiation exposure and warming on cyan...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13377
更新日期：2021-01-04 00:00:00
abstract：:We study the fluorescence lifetime of the well-known 1-pyrene butyric acid (PBA) to assess oxygen concentrations in living cells. The behavior of the probe is first studied in water, ethanol, protein solution and liposome suspension. The Stern-Volmer plot of these solutions is linear, and the bimolecular reaction rate...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-03-11-RA-109
更新日期：2004-09-01 00:00:00
abstract：:These experiments tested the hypothesis that phototropic bending arises when a light gradient across the stem differentially inhibits cell elongation because of direct inhibition of cell elongation by light (the Blaauw hypothesis). Continuous irradiation of dark-grown cucumber seedlings (Cucumis sativus L.) with unil...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1985.tb01642.x
更新日期：1985-01-01 00:00:00
abstract：:The dependence of autofluorescence properties on the metabolic and functional engagement and on the transformation condition was studied on single cells. Normal Galliera rat fibroblasts at low subculture passage (cell strain), at high subculture passage (stabilized cell line), and transformed cell line derived from a ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(1999)069<0364:dofapo>2.3.co;2
更新日期：1999-03-01 00:00:00
abstract：:Gel sequencing experiments with the 5'- and 3'-end-labeled oligonucleotides d(A3GA4GA5GA6GA3G) and d(AT)10 have demonstrated that dimeric adenine photoproducts and thymine-adenine photoadducts constitute alkali-labile lesions in UV-irradiated DNA. On treatment with hot piperidine, DNA strand breakage occurs predominan...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05022.x
更新日期：1994-02-01 00:00:00
abstract：:Effects of solvent, pH and hydrogen bonding with N-methylimidazole (MIm) on the photophysical properties of 1-hydroxyfluorenone (1HOF) have been studied. Fluorescence lifetime, fluorescence quantum yield and triplet yield measurements demonstrated that intersystem crossing was the dominant process in apolar media and ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-01-27RA-067.1
更新日期：2004-07-01 00:00:00
abstract：:The response of normal brain to photodynamic therapy (PDT) was investigated in 62 Fisher rats. The animals were injected i.p. with Photofrin II (12.5 mg/kg). Forty-eight hours following injection, an area of dura 5 mm in diameter over the frontal cortex was photoactivated with red light (632 +/- 2 nm) at 100 mW cm-2, ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1991.tb01992.x
更新日期：1991-07-01 00:00:00
abstract：:This article is a highlight of the study by Stamenkovic and Hanelt in this issue of Photochemistry and Photobiology describing the high-irradiance photophysiology of several strains of freshwater chlorophyte microalgal genus, Cosmarium. These strains exhibit distinct differences in how combined thermal- high irradianc...
journal_title：Photochemistry and photobiology
pub_type： 评论,杂志文章
doi：10.1111/php.12081
更新日期：2013-07-01 00:00:00
abstract：:This review deals with the biophysical aspects of flavin-based photosensors, comprising cryptochromes, LOV (Light, Oxygen and Voltage) and BLUF (Blue Light sensing Using FAD) proteins. Special emphasis is given to structural issues, photocycle quantum yields and energetics, mechanism of the light-triggered reactions, ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2007.00196.x
更新日期：2007-11-01 00:00:00
abstract：:Limited trypsinization of large pea (Pisum sativum cv. Alaska) phytochrome and subsequent size-exclusion chromatography (SEC) in 0.1 M Na phosphate, pH 7.8, yielded a high-molecular-mass aggregate of tryptic fragments of phytochrome. Further SEC in 0.1 M Tris-HCl, pH 7.5, plus various concentrations of NaSCN, indicate...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1989.tb04136.x
更新日期：1989-07-01 00:00:00
abstract：:Vitamin D production in human skin occurs only when incident UV radiation exceeds a certain threshold. From simulations of UV irradiances worldwide and throughout the year, we have studied the dependency of the extent and duration of cutaneous vitamin D production in terms of latitude, time, total ozone, clouds, aeros...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-11-19-RN-375
更新日期：2005-11-01 00:00:00
abstract：:Photodynamic therapy (PDT) is an increasingly popular anticancer treatment that uses photosensitizer, light and tissue oxygen to generate cytotoxic reactive oxygen species (ROS) within illuminated cells. Acting to counteract ROS-mediated damage are various cellular antioxidant pathways. In this study, we combined PDT ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.01022.x
更新日期：2012-01-01 00:00:00
abstract：:Mitochondria have been implicated as a primary subcellular site of porphyrin localization and photodestruction. However, other organelles including the cell membrane, lysosomes and nucleus have been shown to be damaged by hematoporphyrin derivative (HpD) photosensitized destruction as well. In this study we attempted ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1990.tb01736.x
更新日期：1990-04-01 00:00:00
abstract：:The classic method of photometric curves for photosensitivity determination has been extended to the case of photoreversible reactions and applied to the octopus rhodopsin --> acid metarhodopsin photoreaction. In such cases, measurements at one irradiation wavelength yield the sum of the photosensitivities of the forw...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1987.tb04744.x
更新日期：1987-07-01 00:00:00
abstract：:Among beetle luciferases, the pH-sensitive firefly luciferases have been studied extensively. Much less is known about pH-insensitive luciferases, which include click beetle and railroad worm luciferases. Previously, we found that the residues R215 and T226 (N230) are important for green light emission. Here we show t...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)076<0538:tioaga>2.0.co;2
更新日期：2002-11-01 00:00:00
abstract：:Antibodies and antigen binding fragments thereof were photochemically immobilized on surface-modified silicon chips of 5 x 5 mm size. Silicon surface-grafted diazirines and benzophenones formed covalent bonds with the immunoreagents on light activation. Photolithographic immobilization of monoclonal antibodies in aque...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb09867.x
更新日期：1995-06-01 00:00:00
abstract：:This work evaluated the photoprotective and antigenotoxic effects against ultraviolet B (UVB) radiation of flavonoid compounds apigenin, naringenin and pinocembrin. The photoprotective efficacy of these compounds was estimated using in vitro photoprotection indices, and the antigenotoxicity against UVB radiation was e...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13085
更新日期：2019-07-01 00:00:00
abstract：:Spatio-temporal patterns in sun exposure underlie variations in skin cancer incidence and vitamin D deficiency, indicate effectiveness of sun protection programs and provide insights into future health risks. From 558 adults across four regions of Australia (Brisbane (27°S), Newcastle (33°S), Geelong and the Western D...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12044
更新日期：2013-05-01 00:00:00
abstract：:Persister bacteria tolerate bactericidal antibiotics due to transient and reversible phenotypic changes. As these bacteria can limit the effectiveness of antibiotics to eradicate certain infections, their elimination is a relevant issue. Photodynamic therapy seems suitable for this purpose, but phenotypic tolerance to...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12843
更新日期：2018-01-01 00:00:00
abstract：:Analysis of phosphorescence lifetimes using the Stern-Volmer equation is a reliable means of determining quencher concentration for a uniform sample. Methods of analysis for heterogeneous systems are based on the assumption that the excitation is produced by a momentary flash. This condition is an idealization because...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：:Lymphocytes treated with delta-aminolevulinic acid (ALA) can accumulate the photoactive, fluorescent heme precursor, protoporphyrin IX (PpIX). With visible light illumination, PpIX can be used in photodynamic therapy (ALA-PDT) to kill or functionally alter cells. The aim of this study was to characterize the effects o...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
