Surface Functionalization of Green-synthesized Reduced Graphene Oxide with PPIX Enhances Photosensitization of Cancer Cells.

Abstract:

:Photosensitizer-functionalized reduced graphene oxide (rGO) nanoparticles are promising materials for photodynamic therapy in cancer management. In this study, rGO is synthesized by a green route employing glucose as the reducing agent and functionalized with photosensitizer, protoporphyrin IX (PPIX) in a convenient, single-step procedure. PPIX-functionalized rGO exhibits photodynamic effect against cancer cells (HeLa) at 0.001 mg mL-1 under visible light illumination (635 nm). A 50% elimination of HeLa cells after 5 min irradiation is observed while very low phototoxicity (80% cell viability) is noted against normal dermal fibroblast cells. A positive correlation with ROS accumulation and increased expression of caspase-3 in PPIX-functionalized rGO-treated cancer cells is also established. The results evidence a simple and cost-effective route for developing photosensitizer-functionalized rGO for effective and selective killing of cancer cells.

journal_name

Photochem Photobiol

authors

Shuvra Smita S,Das A,Barui A

doi

10.1111/php.13316

subject

Has Abstract

pub_date

2020-11-01 00:00:00

pages

1283-1293

issue

6

eissn

0031-8655

issn

1751-1097

journal_volume

96

pub_type

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