Abstract:
:Photodynamic treatment of promyelocytic K562 cells in the presence of a monoglucosylporphyrin or hematoporphyrin leads to a sequence of events recognized as hallmarks of apoptosis: a drop in mitochondrial potential, concurrent with a drop in ATP level and a decrease in cell respiration, translocation of phosphatidylserine of the plasma membrane, DNA fragmentation, appearance of apoptotic bodies and eventually loss of plasma membrane integrity. The chronology of these events is in accordance with sequential events induced by other known proapoptotic agents; in contrast to these agents that induce apoptosis in a restricted part of the cell population, we observed that the entire cell population (apart from a small percentage of cells that endured rapid necrosis during phototreatment) underwent apoptosis after irradiation in the presence of porphyrins. It appears that photodynamic treatment allows the bypass of early apoptotic signals in K562 cells that are otherwise renowned for their resistance to drug-induced apoptosis (A. McGahon, R. Bissonnette, M. Schmitt, K. M. Cotter, D. R. Green and T. G. Cotter, Blood 83, 1179-1187, 1994). Singlet oxygen is believed to be the proximate reactive species generated by porphyrin illumination. Because this molecule reacts with almost every cellular constituent, the way that singlet oxygen or its reactive oxygen species byproducts trigger apoptosis remains to be elucidated.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Carré V,Jayat C,Granet R,Krausz P,Guilloton Msubject
Has Abstractpub_date
1999-01-01 00:00:00pages
55-60issue
1eissn
0031-8655issn
1751-1097journal_volume
69pub_type
杂志文章abstract::Continuous red light controls starch degradation in turions of Spirodela polyrhiza [Dölger, K., U. K. Tirlapur and K.-J. Appenroth [1997] Photochem. Photobiol. 66, 126-127 (1997)]. This light could be replaced by repeated red light pulses with the reciprocity law fulfilled over a large range of fluence rates. The effe...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2001)073<0077:lisdin>2.0.co;2
更新日期:2001-01-01 00:00:00
abstract::Advanced glycation endproducts (AGEs) have been suggested as photosensitizers that are capable of mediating eye lens photo-damage during aging. In the present work, we investigate the photo-crosslinking and oxidation of bovine lens proteins sensitized by AGEs, with special regard to low oxygen conditions. A mechanisti...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00415.x
更新日期:2009-01-01 00:00:00
abstract::Mitochondria have been implicated as a primary subcellular site of porphyrin localization and photodestruction. However, other organelles including the cell membrane, lysosomes and nucleus have been shown to be damaged by hematoporphyrin derivative (HpD) photosensitized destruction as well. In this study we attempted ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1990.tb01736.x
更新日期:1990-04-01 00:00:00
abstract::In horticultural crops, the use of screens to protect plants is the usual strategy in the Mediterranean area. Screen manufacturers offer a range of netting that vary in their UV-absorbing properties. We compared the photoeffects of seven different screens. Sweet pepper trials were conducted at the Gilat Research Cente...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2009.00657.x
更新日期:2010-03-01 00:00:00
abstract::Elemental sulfur is a low-cost and abundant substance as one of the largest by-products of the oil industry which was widely used in many industrial activities. Cyclo-octasulfur (S8 ) is one of the sulfur allotropes that is a very stable substance in standard conditions. In this study, we report a low-cost and fast me...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13301
更新日期:2020-11-01 00:00:00
abstract::During the past several years there has been debate about the origins of nonexponential intensity decays of intrinsic tryptophan (trp) fluorescence of proteins, especially for single tryptophan proteins (STP). In this review we summarize the data from diverse sources suggesting that time-dependent spectral relaxation ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1562/0031-8655(2000)072<0421:OSRIP>2.0.CO;2
更新日期:2000-10-01 00:00:00
abstract::Photodynamic therapy (PDT) is an established therapeutic modality that uses nonionizing near-infrared light to activate photocytotoxicity of endogenous or exogenous photosensitizers (PSs). An ongoing avenue of cancer research involves leveraging PDT to stimulate antitumor immune responses; however, these effects appea...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13153
更新日期:2020-03-01 00:00:00
abstract::Photodimerization of cocrystals of four bispyridylethylenes and two stilbazoles with urea as a template in the solid state has been investigated following our success with thiourea. Four investigated olefins photodimerized quantitatively to a single dimer in the crystalline state only. The reactivity of urea-olefin cr...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12353
更新日期:2015-05-01 00:00:00
abstract::The photophysical properties of two energy-transfer dyads that are potential candidates for near-infrared (NIR) imaging probes are investigated as a function of solvent polarity. The dyads (FbC-FbB and ZnC-FbB) contain either a free base (Fb) or zinc (Zn) chlorin (C) as the energy donor and a free base bacteriochlorin...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00532.x
更新日期:2009-07-01 00:00:00
abstract::Photodynamic therapy (PDT) using the second-generation photosensitizer phthalocyanine (Pc) 4 causes mitochondrial damage and induces apoptosis through the release of cytochrome c to the cytosol. Another protein of the mitochondrial intermembrane space, Smac/DIABLO (second mitochondria-derived activator of caspase/dire...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2002)076<0217:poptia>2.0.co;2
更新日期:2002-08-01 00:00:00
abstract::The relationship between levels of in vivo accumulated photosensitizer (Photofrin II), photodynamic cell inactivation upon in vitro or in vivo illumination, and changing tumor oxygenation was studied in the radiation-induced fibrosarcoma (RIF) mouse tumor model. In vivo porphyrin uptake by tumor cells was assessed by ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1989.tb04110.x
更新日期:1989-03-01 00:00:00
abstract::By a technique which combines rapid mixing of cells and hematoporphyrin (HP) with a short duration of illumination, the photodynamic inactivation of yeast cells was investigated, particularly, in seeking for the information of the location of HP at the time of action. The fluence-survival curves obtained under the con...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::Albino hairless mice (Skh: HR-1) exposed chronically to sub-erythemal doses of UV radiation display physical, visible and histological alterations. Using narrow bandwidth radiation covering the UV radiation spectrum from 280-380 nm, the wavelength dependence of these alterations was determined. The wavelength dependen...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1989.tb02907.x
更新日期:1989-12-01 00:00:00
abstract::Topical photodynamic therapy is used for a variety of malignant and pre-malignant skin disorders, including Bowen's Disease and Superficial Basal Cell Carcinoma. A haem precursor, typically 5-aminolevulinic acid (ALA), acting as a prodrug, is absorbed and converted by the haem biosynthetic pathway to photoactive proto...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1562/2004-08-23-IR-283
更新日期:2005-07-01 00:00:00
abstract::This paper studies the dye-sensitized photooxidation of tyrosine (tyr) and tyr di- and tripeptides (tyr-tyr and tyr-tyr-tyr) mediated by singlet molecular oxygen (O2[1 delta g]) in alkaline media. Photooxidation quantum efficiencies (phi r) were obtained by determining the overall and reactive rate constants of intera...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::Pheophorbide a-induced photo-oxidation, in vitro, of cytochrome c oxidase and cytochrome c results in irreversible modifications to both protein components. Photo-oxidation of cytochrome c, as exhibited by change in its heme oxidation state, displays exponential kinetics and is detected with a lag period. Both the pho...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1992.tb04229.x
更新日期:1992-02-01 00:00:00
abstract::The photodegradation of the S(+)- and R(-)-ketoprofen (KP) enantiomers in the bovine serum albumin matrix was studied by steady-state photolysis with the use of lambda(irr) > 320 nm and transient absorption spectroscopy with lambda(exc) = 355 nm, at 1/1 and 2/1 KP/BSA molar ratios. R(-)-KP was found to be more labile ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2005-07-11-RA-608
更新日期:2006-01-01 00:00:00
abstract::Vitamin A palmitate has been used in cosmetics; however, studies report that this substance shows photoreactivity that can lead to loss of safety and efficacy. On the other hand, photostabilizers have been used to increase sunscreen photostability and consequently their safety and effectiveness. Thus, this study aimed...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2010.00806.x
更新日期:2010-11-01 00:00:00
abstract::Plastoglobules are lipoprotein particles contained in chloroplasts and other plastids. They have long been regarded as lipid storage droplets. New results now indicate that plastoglobules actively participate in prenylquinone and other metabolic pathways. Structural work shows physical attachment of plastoglobules to ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/j.1751-1097.2008.00459.x
更新日期:2008-11-01 00:00:00
abstract::We recently reported that variations in cellular phototoxicity among a series of alkynyl-substituted zinc trisulfophthalocyanines (ZnPcS3Cn) correlates with their hydrophobicity, with the most amphiphilic derivatives showing the highest cell uptake and phototoxicity. In this study we address the role of the plasma mem...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2005-12-13-RA-752
更新日期:2006-11-01 00:00:00
abstract::We examined the effects of daily exposure to UVB on growth, reproduction and histological characteristics of Daphnia magna over two generations at 20, 22, 25 and 30°C. Animals were exposed to 16 h of UVA and photosynthetically active radiation daily. Treated animals received 6 h of UVB during the light phase. Parental...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2012.01197.x
更新日期:2013-01-01 00:00:00
abstract::Stratospheric ozone depletion has caused an increase in the amount of ultraviolet-B (UV-B) radiation reaching the earth's surface. Numerous investigations have demonstrated that the effect of UV-B enhancements on plants includes reduction in grain yield, alteration in species competition, susceptibility to disease and...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-05-03-RA-156.1
更新日期:2004-07-01 00:00:00
abstract::The proteins induced by heat and other stressors, called heat shock proteins (HSP) or stress proteins, are considered to play a general role in protection from cellular injury. Exposure to UVA (320-400 nm) following application of 8-methoxypsoralen (8-MOP), termed PUVA is commonly used in the field of dermatology. In ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1993.tb04974.x
更新日期:1993-12-01 00:00:00
abstract::On direct photoexcitation, subpicosecond time-resolved absorption spectroscopy revealed that the 1B(u)-type singlet excited state of all-trans-lycopene in chloroform was about seven times more efficient than all-trans-beta-carotene in generating the radical cation. The time constant of radical cation generation from t...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-04-22-RA-147
更新日期:2004-09-01 00:00:00
abstract::Seafarers working on decks of vessels at low latitudes are exposed to extremely high solar UV radiation. Their risk of developing skin cancer may be enhanced. Solar erythemal UV irradiance and exposure were measured for the first time on merchant vessels going along typical international routes at low latitudes. The m...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12144
更新日期:2013-11-01 00:00:00
abstract::Positively charged zinc containing or metal free phthalocyanines 6a-c and 7a-c were prepared via a three step procedure starting from 4-nitrophthalonitrile. The phthalocyanines contain alkyl chains of different length in order to influence the hydrophilic vs lipophilic character of the compounds. The partition between...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1990.tb01721.x
更新日期:1990-03-01 00:00:00
abstract::Schinus terebinthifolius is a plant rich in phenolic compounds, which have antioxidant properties and can provide new opportunities for treatment and prevention of diseases mediated by ultraviolet radiation like photoaging and skin cancer. The aim of this study was to evaluate the photoprotective potential and ex vivo...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12419
更新日期:2015-05-01 00:00:00
abstract::Light signals trigger precise changes in gene expression networks that activate distinctive developmental programs in plants. The transcriptome is shaped at different stages, both by the regulation of gene expression and also by posttranscriptional mechanisms that alter the sequence or abundance of the transcripts gen...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/php.12762
更新日期:2017-05-01 00:00:00
abstract::As a new class of sequence-specific regulators of gene expression, the microRNAs (miRNA) form a regulatory network with growth factors and transcription factors participating in various biological processes. It is now being recognized that the various key processes involved in cancer induction are under the control of...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/php.12346
更新日期:2015-01-01 00:00:00
abstract::Recent developments in optical technologies have the potential to improve the speed and accuracy of screening and diagnosis of curable precancerous lesions and early cancer, thereby decreasing the costs of detection and management of epithelial malignancies. The development of molecular-specific contrast agents for ma...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/fr-03-15.1
更新日期:2004-03-01 00:00:00