Abstract：
:Mitochondria have been implicated as a primary subcellular site of porphyrin localization and photodestruction. However, other organelles including the cell membrane, lysosomes and nucleus have been shown to be damaged by hematoporphyrin derivative (HpD) photosensitized destruction as well. In this study we attempted to follow the translocation of the fluorescent components of HpD in human bladder tumor cells (MGH-U1) in culture to determine whether specific subcellular localization occurs over time. Following a 30 min exposure to HpD the cellular fluorescence was examined immediately and 1, 2, 4, and 24 h after HpD removal using fluorescence microscopy and an interactive laser cytometer. The in vitro translocation of dye appeared to be fairly rapid with fluorescence present at the cell membrane and later (1-2 h) within a perinuclear area of the cytoplasm. To determine whether HpD had become concentrated into a specific subcellular organelle, these fluorescence distribution patterns were compared with fluorescent marker dyes specific for mitochondria, endoplasmic reticulum and other membranous organelles. The HpD fluorescence did not appear to be as discrete as the dyes specific for mitochondria or endoplasmic reticulum but appeared similar to the diffuse cytomembrane stain. Finally, the interaction between the fluorescent components of HpD and the cellular constituents was evaluated using a "fluorescence redistribution after photobleaching" technique. The results indicated that the mean lateral diffusion for HpD in MGH-U1 cells was 1.05 x 10(-8) cm2/s, a rate closer to that of lipid diffusion (10(-8)) than that of protein diffusion (10(-10)).(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Shulok JR,Wade MH,Lin CWdoi
10.1111/j.1751-1097.1990.tb01736.xsubject
Has Abstractpub_date
1990-04-01 00:00:00pages
451-7issue
4eissn
0031-8655issn
1751-1097journal_volume
51pub_type
杂志文章abstract：:Protoporphyrin IX dimethyl ester (PME), a dimethyl esterification of protoporphyrin IX (PpIX), exhibits higher intracellular uptake into NPC/CNE2 cells, a poorly differentiated human nasopharyngeal carcinoma, than does PpIX. Phototoxicity studies reveal PME to be a more potent photosensitizer than is PpIX, at the earl...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)076<0678:acopia>2.0.co;2
更新日期：2002-12-01 00:00:00
abstract：:We analyzed the photoinactivation of the membrane functions of bacteria and erythrocytes induced by xanthene dyes. The dyes tested were rose bengal, phloxine B, erythrosine B and eosin B. These dyes induced the leakage of K(+) from Staphylococcus aureus cells within minutes of photoirradiation, in the order of rose be...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2012.01080.x
更新日期：2012-03-01 00:00:00
abstract：:The phototoxic anti-hyperlipoproteinemic drug fenofibrate was found to be photolabile under aerobic and anaerobic conditions. Irradiation under argon of a methanol solution of this drug produced the photoproducts isopropyl 4-(1-[4-chlorophenyl]-1,2-dihydroxy)ethylphenoxyisobutyrate, 1,2-bis(4-chlorophenyl)-1,2bis (4-[...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb04917.x
更新日期：1993-10-01 00:00:00
abstract：:We study the fluorescence lifetime of the well-known 1-pyrene butyric acid (PBA) to assess oxygen concentrations in living cells. The behavior of the probe is first studied in water, ethanol, protein solution and liposome suspension. The Stern-Volmer plot of these solutions is linear, and the bimolecular reaction rate...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-03-11-RA-109
更新日期：2004-09-01 00:00:00
abstract：:Systemic injection of hematoporphyrin derivative (HpD) in contribution with visible light (red or blue-green) delivered by laser was used to treat a patient with psoriasis. The psoriatic lesions responded vigorously to laser treatments, forming eschars by 1 week post irradiation. In contrast, only minimal erythema was...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1987.tb04739.x
更新日期：1987-07-01 00:00:00
abstract：:The mechanism of caspase-3-dependent apoptosis induced by photodynamic therapy (PDT) of cultured Chinese hamster V79 cells with pheophorbide a (PPa) was investigated. The PPa-PDT induced rapid apoptosis within 30 min after irradiation of cells. This apoptosis was inhibited by the 1O2 quencher N3- and caspase-3 inhibit...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
abstract：:The recently discovered photo-activated adenylyl cyclase (mPAC from Microcoleus chthonoplastes) is the first PAC that owes a light-, oxygen- and voltage-sensitive (LOV) domain for blue-light sensing. The photoreaction of the mPAC receptor was studied by time-resolved UV/vis and light-induced Fourier transform infrared...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12746
更新日期：2017-05-01 00:00:00
abstract：:The retina and retinal pigment epithelium contain a number of retinoids in a metabolic pathway that eventually forms the visual pigments. This study investigates the photochemistry of those retinoids that may contribute to light-induced damage to the retina. These include retinal (RAL), retinol (ROL), retinylpalmitate...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1996.tb05673.x
更新日期：1996-05-01 00:00:00
abstract：:The incidence of skin cancer is increasing rapidly, particularly in the Caucasian population. Epidemiological and experimental studies demonstrated that ultraviolet radiation (UVR) is the primary cause for the increasing incidence of skin cancer. It is well known that UV irradiation induces DNA damage. If the damage i...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：:The aim of this investigation is the evaluation of DNA interaction of with tetraruthenated porphyrin (TRP) and of DNA damage in the presence of light. Direct-fluorescence and electronic absorption measurements after incubation of DNA with TRP indicate strong binding between pBR322 DNA or calf thymus DNA with the modif...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1996.tb03024.x
更新日期：1996-03-01 00:00:00
abstract：:Stratospheric ozone depletion has caused an increase in the amount of ultraviolet-B (UV-B) radiation reaching the earth's surface. Numerous investigations have demonstrated that the effect of UV-B enhancements on plants includes reduction in grain yield, alteration in species competition, susceptibility to disease and...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-05-03-RA-156.1
更新日期：2004-07-01 00:00:00
abstract：:Skin cancer studies depend on questionnaires to estimate exposure to ultraviolet light and subsequent risk but are limited by recall bias. We investigate the feasibility of developing a short checklist of categories comprising outdoor activities that can improve sun exposure questionnaires for use in epidemiologic stu...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2008.00393.x
更新日期：2009-01-01 00:00:00
abstract：:This paper studies the dye-sensitized photooxidation of tyrosine (tyr) and tyr di- and tripeptides (tyr-tyr and tyr-tyr-tyr) mediated by singlet molecular oxygen (O2[1 delta g]) in alkaline media. Photooxidation quantum efficiencies (phi r) were obtained by determining the overall and reactive rate constants of intera...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:Eumelanin plays a variety of important physiological roles in human skin. However, its structure and fundamental properties still remain poorly understood. Although the absorbance of eumelanin is broad and reveals little about its structure, a variety of techniques have revealed the presence of a disordered array of c...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00186.x
更新日期：2007-11-01 00:00:00
abstract：:We report the spectroscopic properties of fluorescein, x-rhodamine, tetramethyl-rhodamine, attached to single strand, duplex DNA, and to the digestion products by DNAse I. The properties reported include: molar absorptivity, quantum yield, absorbance and fluorescence spectra, fluorescence lifetime, intrinsic lifetime ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2009.00663.x
更新日期：2010-03-01 00:00:00
abstract：:It was shown that psoralen + UV-A inhibits the chemotactic activity of polymorphonuclear neutrophils towards anaphylatoxin C5a des Arg. This reaction required oxygen and there is a high possibility that the active oxygen species was in a singlet state. Oxygen did not act directly on C5a des Arg, but rather produced ox...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：:Use of near infrared instead of visible light would markedly improve tissue penetration, making larger tumors candidates for photochemotherapy. Because common photesensitizers exhibit virtually no absorption in this wavelength region, conditions are required where the simultaneous action of two photons is possible. He...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb05277.x
更新日期：1995-08-01 00:00:00
abstract：:Ultraviolet-A irradiation of a suprofen (2-[4-2(2-thenoyl) phenyl]propionic acid) (SPF) buffered solution (pH 7.4) in the presence of supercoiled pBR322 DNA leads to single strand breaks with the formation of an open circular form and subsequent linearization of the plasmid. On the basis of agarose gel electrophoresis...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb05252.x
更新日期：1995-07-01 00:00:00
abstract：:Photoreduction of cytochrome c (Cyt c) has been reinvestigated using femtosecond-to-nanosecond transient absorption and stationary spectroscopy. Femtosecond spectra of oxidized Cyt c, recorded in the probe range 270-1000 nm, demonstrate similar evolution upon 266 or 403 nm excitation: an ultrafast 0.3 ps internal conv...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00219.x
更新日期：2008-01-01 00:00:00
abstract：:Photolysis of ester 1 in argon-saturated methanol and acetonitrile does not produce any product, whereas irradiation of 1 in oxygen-saturated methanol yields peroxide 2. Laser flash photolysis studies demonstrate that 1 undergoes intramolecular H atom abstraction to form biradical 3 (λmax ~340 nm), which intersystem c...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12590
更新日期：2016-05-01 00:00:00
abstract：:Photosensitizer-functionalized reduced graphene oxide (rGO) nanoparticles are promising materials for photodynamic therapy in cancer management. In this study, rGO is synthesized by a green route employing glucose as the reducing agent and functionalized with photosensitizer, protoporphyrin IX (PPIX) in a convenient, ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13316
更新日期：2020-11-01 00:00:00
abstract：:The octahedral rhodium complex, cis-dichloro bis(1,10 phenanthroline)rhodium(III) chloride (BISPHEN), is known to form covalent linkages with DNA involving the attachment of the metal to a base. In order to determine the sequence selectivity of this chemistry, solutions of the complex containing one of the double-stra...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)072<0731:hsawtp>2.0.co;2
更新日期：2000-12-01 00:00:00
abstract：:Light emitted by electronic photographic flash units is shown to damage bacteria and human skin fibroblasts deficient in repair systems, with survival curves very similar to those produced by 254 nm short UV. The lesions induced by these flashes are as photorepairable by the photolyase enzyme as those induced by 254 n...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1996.tb03103.x
更新日期：1996-09-01 00:00:00
abstract：:Photodynamic therapy (PDT), carried out at low fluence rates, may enhance tumor response as well as affect treatment selectivity. We have studied the effects of fluence rate on the response of the murine radiation-induced fibrosarcoma (RIF) to PDT using Photofrin (5 mg/kg). Tumor response was tested over a large range...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：:The photosolvolysis of several biphenyl methanols (Ph-PhCH[Ph]OH) substituted with hydroxy or methoxy groups on the benzene ring not containing the -CH(Ph)OH moiety has been studied in aqueous solution. This work is a continuation of our studies of photosolvolysis of hydroxy-substituted arylmethanols that generate qui...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2005-02-17-RA-444
更新日期：2006-01-01 00:00:00
abstract：:The total ozone column (TOC) is the most significant quantity for estimating the erythemally effective UV radiation under clear sky conditions. Uncertainties in TOC measurements and a limited spatial and temporal resolution therefore influence the quality of calculated erythemally effective radiation. The UV Index, th...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00285.x
更新日期：2008-09-01 00:00:00
abstract：:Herein, we present a study of the pharmacokinetics and biodistribution of a butadiyne-linked conjugated porphyrin dimer (Oxdime) designed to have high near-infrared (NIR) 2-photon absorption cross-section for photodynamic therapy (PDT). Changes in biodistribution over time were monitored in mice carrying B16-F10 melan...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2012.01194.x
更新日期：2012-11-01 00:00:00
abstract：:Oculocutaneous albinism (OCA) is a genetically inherited autosomal recessive condition. Individuals with OCA lack melanin and therefore are susceptible to the harmful effects of solar ultraviolet radiation, including extreme sun sensitivity, photophobia and skin cancer. OCA is a grave public health issue in sub-Sahara...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.12359
更新日期：2015-01-01 00:00:00
abstract：:A careful study of the linoleic acid hydroperoxide (LOOH) profile obtained upon peroxidation of linoleic acid (LA) photosensitized by tiaprofenic acid (TPA) and analogous ketones has been undertaken to distinguish between type-I and type-II photoperoxidation mechanisms. 1,4-Cyclohexadiene and 1,2-dimethylcyclohexa-2,5...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2001)073<0359:molppb>2.0.co;2
更新日期：2001-04-01 00:00:00
abstract：:The broad-band normal incidence UVB beam radiation has been measured at Neve Zohar, Dead Sea basin, using a prototype tracking instrument composed of a Model 501A UV-Biometer mounted on an Eppley Solar Tracker Model St-1. The diffuse and beam fraction of the solar global UVB radiation have been determined using the co...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2010.00842.x
更新日期：2011-01-01 00:00:00
