Abstract:
:We report the spectroscopic properties of fluorescein, x-rhodamine, tetramethyl-rhodamine, attached to single strand, duplex DNA, and to the digestion products by DNAse I. The properties reported include: molar absorptivity, quantum yield, absorbance and fluorescence spectra, fluorescence lifetime, intrinsic lifetime (tau0), static quenching (S) and the Förster critical distances (R0) between fluorescein and x-rhodamine or tetramethyl-rhodamine (acceptors). These spectroscopic properties depend strongly on the local dye environment. Fluorescein was studied: (1) attached to biotin (BF), (2) BF bound to avidin; and attached to two positions in DNA. X-rhodamine and tetramethyl-rhodamine were studied as free dyes and attached at the 5'-end of DNA. We propose a general method to determine the molar absorptivity and tau0 of a dye attached to DNA based on the reaction of a biotinylated and dye-labeled oligomer with standardized avidin. The molar absorptivity of a second dye attached to a DNA duplex can be obtained by comparing spectra of doubly and singly labeled sequences. S, arising from dye-DNA interactions can then be determined. R0 for free and attached dyes showed differences from 1.1 to 4.2 A. We present evidence for the direct interaction of dyes attached to the termini of various single-stranded DNA sequences.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Delgadillo RF,Parkhurst LJdoi
10.1111/j.1751-1097.2009.00663.xsubject
Has Abstractpub_date
2010-03-01 00:00:00pages
261-72issue
2eissn
0031-8655issn
1751-1097pii
PHP663journal_volume
86pub_type
杂志文章abstract::Photosynthetic bacteria are attractive for biotechnology because they produce no oxygen and so H2 -production is not inhibited by oxygen as occurs in oxygenic photoorganisms. Rhodopseudomonas palustris and Afifella marina containing BChl a can use irradiances from violet near-UV (VNUV) to orange (350-650 nm) light and...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12124
更新日期:2013-09-01 00:00:00
abstract::The recently discovered photo-activated adenylyl cyclase (mPAC from Microcoleus chthonoplastes) is the first PAC that owes a light-, oxygen- and voltage-sensitive (LOV) domain for blue-light sensing. The photoreaction of the mPAC receptor was studied by time-resolved UV/vis and light-induced Fourier transform infrared...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12746
更新日期:2017-05-01 00:00:00
abstract::The protein environments surrounding the retinal tune electronic absorption maximum from 350 to 630 nm. Hybrid quantum mechanical/molecular mechanical (QM/MM) methods can be used in calculating excitation energies of retinal in its native protein environments and in studying the molecular basis of spectral tuning. We ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/j.1751-1097.2008.00308.x
更新日期:2008-07-01 00:00:00
abstract::Immunosuppressive doses of solar-simulated UV radiation activate lymph node B cells that can suppress primary immunity by inhibiting the function of dendritic cells. The aim of this study was to determine the waveband responsible for activation of these suppressor B cells. We exposed C57BL/6 mice to various doses of e...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2005-04-20-RA-495
更新日期:2005-11-01 00:00:00
abstract::UV rays may cause several degrees of skin damage, which makes sunscreen research necessary. In addition, skin sensitivity shows daily variations, which can interfere in the detection of the efficacy of the filters. Here, we studied the UV-induced erythema in hairless rats at two times of the day (light and darkness) u...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12670
更新日期:2017-03-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1994.tb05039.x
更新日期:1994-03-01 00:00:00
abstract::Urban environments can have high-risk spaces that can provide excess personal sun exposure, such as urban or street canyons, and the spaces between buildings, among others. In these urban spaces, sun exposure can be high or low depending on several factors. Polysulphone film (PSF) was used to assess possible daily sol...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13291
更新日期:2020-09-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12595
更新日期:2016-07-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1993.tb04974.x
更新日期:1993-12-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2001)073<0077:lisdin>2.0.co;2
更新日期:2001-01-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2012.01211.x
更新日期:2013-01-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1996.tb03008.x
更新日期:1996-02-01 00:00:00
abstract::Intracellular targets for the photosensitizer alpha-terthienyl (alpha T) were examined by fluorescence microscopy and microfluorospectrometry using human nonkeratinized buccal cells. Intracellular distribution of alpha T was observed as fluorescent patches widely dispersed in the cytoplasm. The distribution of the flu...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1993.tb09213.x
更新日期:1993-05-01 00:00:00
abstract::Sapphyrins and a series of related porphyrinoid macrocycles have been investigated as potential agents for the treatment of leishmaniasis. The effectiveness of the compounds was evaluated in vitro upon incubation with Leishmania tarentolae or L. panamensis amastigotes and promastigotes. Their effectiveness was also as...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2011.01034.x
更新日期:2012-01-01 00:00:00
abstract::This article is a highlight of the study by Stamenkovic and Hanelt in this issue of Photochemistry and Photobiology describing the high-irradiance photophysiology of several strains of freshwater chlorophyte microalgal genus, Cosmarium. These strains exhibit distinct differences in how combined thermal- high irradianc...
journal_title:Photochemistry and photobiology
pub_type: 评论,杂志文章
doi:10.1111/php.12081
更新日期:2013-07-01 00:00:00
abstract::The kinetics of transients formed under photoexcitation of benzophenone (B) dissolved in three different polymers was studied by ns laser flash photolysis. These polymers were the soft rubbers poly (ethylene-co-butylene) (EB), polystyrene block-poly(ethylene-ran-butylene)-block-polystyrene (SEBS) and hard polystyrene ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12170
更新日期:2014-03-01 00:00:00
abstract::Near-infrared spectroscopy has been used to quantify the composition of healthy female breast tissue in vivo. By collecting transillumination spectra in the wavelength range 680-1100 nm at 7-9 positions on the breasts of five female volunteers, an attempt was made to quantify the intra- and intersubject variability of...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::A 24 h preculture of HT29-18 human colonic adenocarcinoma cells with the sterol synthesis inhibitor lovastatin at concentrations of 0.1-0.5 microM markedly increased the photocytotoxic effect of photofrin II delivered to cells by low density lipoproteins. Under the same conditions, LDL binding and photofrin II (PII) u...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1993.tb02303.x
更新日期:1993-02-01 00:00:00
abstract::The retinal pigment epithelium (RPE) is a highly metabolic layer of postmitotic cells lining Bruch's membrane in the retina. While these cells contain endogenous photosensitizers that mediate blue light-induced damage, it has also been shown that blue light exposure damages mitochondrial DNA in RPE cells resulting in ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12683
更新日期:2017-03-01 00:00:00
abstract::Two-photon laser scanning microscopy (TPLSM) of endogenous reduced nicotinamide adenine dinucleotide (NAD(P)H) provides important information regarding the cellular metabolic state. When imaging the punctate mitochondrial fluorescence originating from NAD(P)H in a rat basophilic leukemia (RBL) cell at low laser powers...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-08-05-RA-263
更新日期:2005-03-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2001)073<0014:dbftot>2.0.co;2
更新日期:2001-01-01 00:00:00
abstract::A key challenge for microbiology is to understand how evolution has shaped the wiring of regulatory networks. This is amplified by the paucity of information of power-spectra of physicochemical stimuli to which microorganisms are exposed. Future studies of genome evolution, driven by altered stimulus regimes, will the...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/php.12499
更新日期:2015-09-01 00:00:00
abstract::Topical photodynamic therapy is used for a variety of malignant and pre-malignant skin disorders, including Bowen's Disease and Superficial Basal Cell Carcinoma. A haem precursor, typically 5-aminolevulinic acid (ALA), acting as a prodrug, is absorbed and converted by the haem biosynthetic pathway to photoactive proto...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1562/2004-08-23-IR-283
更新日期:2005-07-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2001)074<0164:ivsotp>2.0.co;2
更新日期:2001-08-01 00:00:00
abstract::Advanced glycation endproducts (AGEs) have been suggested as photosensitizers that are capable of mediating eye lens photo-damage during aging. In the present work, we investigate the photo-crosslinking and oxidation of bovine lens proteins sensitized by AGEs, with special regard to low oxygen conditions. A mechanisti...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00415.x
更新日期:2009-01-01 00:00:00
abstract::Generating accurate population-specific public health messages regarding sun protection requires knowledge about seasonal variation in sun exposure in different environments. To address this issue for a subtropical area of Australia, we used polysulphone badges to measure UVR for the township of Nambour (26 degrees la...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2009.00686.x
更新日期:2010-03-01 00:00:00
abstract::Action spectroscopy has a long history and is of central importance to photobiological studies. Action spectra were among the first assays to point to chlorophyll as the molecule most responsible for plant growth and to DNA as the genetic material. It is useful to construct action spectra early in the investigation of...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/j.1751-1097.1991.tb02103.x
更新日期:1991-11-01 00:00:00
abstract::During the maturation of red wines, the anthocyanins of grapes are transformed into pyranoanthocyanins, which possess a pyranoflavylium cation as their basic chromophore. Photophysical properties of the singlet and triplet excited states of a series of synthetic pyranoflavylium cations were determined at room temperat...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12973
更新日期:2019-01-01 00:00:00
abstract::Ultraviolet A radiation participates in cytotoxicity and carcinogenesis of the skin by a mechanism involving the generation of reactive oxygen species. Endogenous antiradical defense systems utilize metalloenzymes including Se-dependent glutathione peroxidase and Cu and Zn superoxide dismutase. The aim of the present ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1993.tb04930.x
更新日期:1993-10-01 00:00:00