Abstract：
:The proteins induced by heat and other stressors, called heat shock proteins (HSP) or stress proteins, are considered to play a general role in protection from cellular injury. Exposure to UVA (320-400 nm) following application of 8-methoxypsoralen (8-MOP), termed PUVA is commonly used in the field of dermatology. In order to understand the induction of HSP in PUVA-treated human skin, indirect immunofluorescence using a monoclonal antibody specific for the 72 kDa HSP (HSP 72) was carried out in organ-cultured normal human skin that was treated with PUVA. When the organ-cultured skin was treated at 37 degrees for 1 h with 8-MOP at a final concentration of 10 or 100 micrograms/mL and exposed to UVA (51.3 kJ/m2), nuclear immunofluorescence of HSP 72 was detected in the epidermal cells 12 h after UVA irradiation. In contrast, the induction of HSP 72 was not detected either by UVA irradiation or 8-MOP treatment. These results suggest that PUVA treatment is one of the stressors for human skin, and DNA damage caused by PUVA induces HSP 72.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Muramatsu T,Yamashina Y,Tada H,Kobayashi N,Yamaji M,Ohno H,Shirai T,Takahashi A,Ohnishi Tdoi
10.1111/j.1751-1097.1993.tb04974.xsubject
Has Abstractpub_date
1993-12-01 00:00:00pages
809-12issue
6eissn
0031-8655issn
1751-1097journal_volume
58pub_type
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