Abstract:
:A spectral resolution procedure was used to resolve the absorption, excitation and emission spectra of the fluorescein monoanion in a number of solvent-water mixtures. This permitted an analysis of the effect of the solvent environment on the spectral properties of the monoanion and on the lactone/monoanion/dianion transitions of fluorescein. The monoanion excitation and emission spectra show relatively small changes with changing environment, a behavior that is related to the hydrogen-bonding environment of the solvent-water mixtures. There is also a general increase in the quantum yield of the monoanion from 0.36 in water to values up to 0.49 in the solvent-water mixtures. The presence of solvent also results in a general increase in the lactone content and in the monoanion:dianion and lactone:monoanion ratios. General polarity effects alone cannot account for the observed effects on the prototropic transitions indicating that specific solute-solvent effects involving hydrogen bonding perturb the prototropic equilibria of fluorescein.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Klonis N,Sawyer WHdoi
10.1562/0031-8655(2000)072<0179:eoswmo>2.0.co;2subject
Has Abstractpub_date
2000-08-01 00:00:00pages
179-85issue
2eissn
0031-8655issn
1751-1097journal_volume
72pub_type
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