Effect of solvent-water mixtures on the prototropic equilibria of fluorescein and on the spectral properties of the monoanion

Abstract:

:A spectral resolution procedure was used to resolve the absorption, excitation and emission spectra of the fluorescein monoanion in a number of solvent-water mixtures. This permitted an analysis of the effect of the solvent environment on the spectral properties of the monoanion and on the lactone/monoanion/dianion transitions of fluorescein. The monoanion excitation and emission spectra show relatively small changes with changing environment, a behavior that is related to the hydrogen-bonding environment of the solvent-water mixtures. There is also a general increase in the quantum yield of the monoanion from 0.36 in water to values up to 0.49 in the solvent-water mixtures. The presence of solvent also results in a general increase in the lactone content and in the monoanion:dianion and lactone:monoanion ratios. General polarity effects alone cannot account for the observed effects on the prototropic transitions indicating that specific solute-solvent effects involving hydrogen bonding perturb the prototropic equilibria of fluorescein.

journal_name

Photochem Photobiol

authors

Klonis N,Sawyer WH

doi

10.1562/0031-8655(2000)072<0179:eoswmo>2.0.co;2

subject

Has Abstract

pub_date

2000-08-01 00:00:00

pages

179-85

issue

2

eissn

0031-8655

issn

1751-1097

journal_volume

72

pub_type

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