Abstract:
:Singlet oxygen ((1)O(2)) is the most important cytotoxic agent in photodynamic therapy (PDT). The feasibility of using a chemiluminescence (CL) probe, 3,7-dihydro-6-[4-(2-(N'-(5-fluoresceinyl)thioureido)ethoxy)phenyl]-2-methylimidazo{1,2-a}pyrazin-3-one sodium salt (fluoresceinyl Cypridina luciferin analog, FCLA), to monitor (1)O(2) production during PDT is evaluated in vitro. Lymphoma cells were treated with various protocols of PDT. The results show that the FCLA-CL production during PDT is linearly related to the corresponding cytotoxicity, regardless of the treatment protocol. With minimum cytotoxicity and interference to the PDT treatment outcome, the FCLA-CL system is an effective means to quantify PDT (1)O(2) production and may provide an alternative real-time dosimeter.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Qin Y,Xing D,Luo S,Zhou J,Zhong X,Chen Qdoi
10.1562/2005-05-20-RA-536subject
Has Abstractpub_date
2005-11-01 00:00:00pages
1534-8issue
6eissn
0031-8655issn
1751-1097pii
2005-05-20-RA-536journal_volume
81pub_type
杂志文章abstract::More scheduled outdoor stay is increasingly advocated for school children. This study measured 2(nd) , 5(th) and 8(th) graders' erythemal UV-exposure in September, March and May at four Swedish schools. We related those exposures, as fractions of total available ambient radiation, to the schools outdoor environments d...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12540
更新日期:2016-01-01 00:00:00
abstract::We study the fluorescence lifetime of the well-known 1-pyrene butyric acid (PBA) to assess oxygen concentrations in living cells. The behavior of the probe is first studied in water, ethanol, protein solution and liposome suspension. The Stern-Volmer plot of these solutions is linear, and the bimolecular reaction rate...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-03-11-RA-109
更新日期:2004-09-01 00:00:00
abstract::Near-UV (337 nm) photoactivation of the 5-hydroxytryptophan decarboxylation reaction producing serotonin has been observed. The photoactivation effect was investigated as a function of fluence rate and fluence, and pH. Photoactivation of decarboxylase activity was found to occur at nearly neutral pH values (low activi...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1989.tb09197.x
更新日期:1989-04-01 00:00:00
abstract::In current clinical practice, photodynamic therapy (PDT) is carried out with prescribed drug doses and light doses as well as fixed drug-light intervals and illumination fluence rates. This approach can result in undesirable treatment outcomes of either overtreatment or undertreatment because of biological variations ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2002)075<0398:mpfapb>2.0.co;2
更新日期:2002-04-01 00:00:00
abstract::Flash-induced absorbance spectroscopy was used to analyze the proton uptake and electron transfer properties of photosynthetic reaction centers (RC) of Rhodobacter capsulatus that have been genetically modified near the primary quinone electron acceptor (Q(A)). M246Ala and M247Ala, which are symmetry-related to the po...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2002)075<0126:puorcr>2.0.co;2
更新日期:2002-02-01 00:00:00
abstract::Excessive sun exposure can cause sunburn, suntan, skin photoaging, and even skin cancer. Skin photoaging conflicts with the human pursuit of a young and beautiful appearance. Some research data indicate that the incidence of skin cancer in the Chinese has been increasing, although it remains lower than in whites. To e...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12623
更新日期:2016-09-01 00:00:00
abstract::We calculated the integrated UVB and plant damage irradiances for Argentina, a country in the Southern Hemisphere spread over a large latitudinal range. The irradiances were calculated for clear sky days using the Madronich code for the average conditions of the months corresponding to the summer and winter solstices ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::Phototropin is a blue light-activated photoreceptor that plays a dominant role in the phototropism of plants. The protein contains two subunits that bind flavin mononucleotide (FMN), which are responsible for the initial steps of the light-induced reaction. It has been proposed that the photoexcited flavin molecule ad...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2003)077<0101:aiqcio>2.0.co;2
更新日期:2003-01-01 00:00:00
abstract::In photodynamic therapy with topically applied delta-aminolevulinic acid porphyrins are acting as photosensitizers. The profile of porphyrin metabolites in normal or in neoplastic skin after administration of delta-aminolevulinic acid has not been determined in detail yet. Thus, to study porphyrin biosynthesis in huma...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1997.tb03146.x
更新日期:1997-07-01 00:00:00
abstract::The retinal protonated Schiff base of bacteriorhodopsin is photoreactive to reducing agents such as NaBH4. In the present work we have studied the effect of different protein hydration levels on the photoreductive reaction, as well as the consequences of preventing isomerization around the critical C13=C14 retinal dou...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2002)075<0668:pobsba>2.0.co;2
更新日期:2002-06-01 00:00:00
abstract::Ring-fused retinal analogs were designed to examine the hula-twist mode of the photoisomerization of the 9-cis retinylidene chromophore. Two 9-cis retinal analogs, the C11-C13 five-membered ring-fused and the C12-C14 five-membered ring-fused retinal derivatives, formed the pigments with opsin. The C11-C13 ring-fused a...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2002)076<0606:coosot>2.0.co;2
更新日期:2002-12-01 00:00:00
abstract::The kinetics of transients formed under photoexcitation of benzophenone (B) dissolved in three different polymers was studied by ns laser flash photolysis. These polymers were the soft rubbers poly (ethylene-co-butylene) (EB), polystyrene block-poly(ethylene-ran-butylene)-block-polystyrene (SEBS) and hard polystyrene ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12170
更新日期:2014-03-01 00:00:00
abstract::Within this study, the erythemal ultraviolet (UV) exposure received by different parts of the body during four different activities is determined. Optoelectronic devices were used to measure the erythemal UV exposure at 10 different positions of the body. The measuring devices were fixed on the forehead, on the should...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12085
更新日期:2013-07-01 00:00:00
abstract::Iranian firefly larvae, Lampyris turkestanicus collected from north of Iran and their luciferin were analyzed by HPLC, TLC, MS and spectroscopic Fourier transform ([FT]-IR, FT-NMR, UV and fluorescence) methods. The results showed that luciferin in L turkestanicus lanterns was the same as in the American firefly, Photi...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1996.tb01841.x
更新日期:1996-11-01 00:00:00
abstract::This paper focuses on the estimation of the UV Index (UVI) for all sky conditions using a simple analytical parameterization involving three independent variables: the solar zenith angle, the total ozone column and the clearness index. Measurements of the UVI made at Badajoz and Cáceres (Southwestern Spain) from Janua...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2010.00860.x
更新日期:2011-03-01 00:00:00
abstract::This paper analyzes the influence of infrared radiation (IR) on regeneration, after autotomy of limb buds of Neohelice granulata and consequently the time molt. Eyestalks were ablated to synchronize the start of molt. Afterward, animals were autotomized of five pereopods and divided into control and irradiated groups....
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2009.00570.x
更新日期:2009-09-01 00:00:00
abstract::Solar radiation is known to be a major contributor to the development of skin cancer. Most sunscreen formulations, including those with broad spectrum, offer minimal protection in long-wavelength ultraviolet A1 (UVA1; 370-400 nm) and visible light (VL; 400-700 nm) domain. There is limited information regarding the imp...
journal_title:Photochemistry and photobiology
pub_type: 信件
doi:10.1111/php.13143
更新日期:2019-11-01 00:00:00
abstract::We previously reported that Shewanella oneidensis MR-1 is extremely sensitive to natural solar radiation (NSR). Here we analyzed the global transcriptional profile of MR-1 during a 1-h recovering period after exposure to ambient solar light at a dose that yields about 20% survival rate on a Luria-Bertani (LB) plate. W...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2005-04-15-RA-490
更新日期:2005-11-01 00:00:00
abstract::Pterins are heterocyclic compounds with important biological functions, and most of them may exist in two acid-base forms in the pH range between 3 and 13 in aqueous solution. In this work, the photophysical properties of acid and basic forms of six compounds of the pterin family (6-hydroxymethylpterin [HPT], 6-methyl...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2005-05-10-RA-522
更新日期:2005-09-01 00:00:00
abstract::Two-photon laser scanning microscopy (TPLSM) of endogenous reduced nicotinamide adenine dinucleotide (NAD(P)H) provides important information regarding the cellular metabolic state. When imaging the punctate mitochondrial fluorescence originating from NAD(P)H in a rat basophilic leukemia (RBL) cell at low laser powers...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-08-05-RA-263
更新日期:2005-03-01 00:00:00
abstract::The phototoxic effects of demethylchlortetracycline (DMCT) with UVA radiation on isolated rat liver mitochondria were studied. DMCT at concentration of 21.5 microM with 5.53 x 10(-2) J cm-3 of UVA was found to be a potent uncoupler of oxidative phosphorylation. DMCT alone also uncoupled mitochondria but at higher conc...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1990.tb01700.x
更新日期:1990-02-01 00:00:00
abstract::1-Hydroxybenzotriazole and 1-hydroxypyridine-2-thione were incorporated as ligands with the cluster Ru3(CO)10 (NCMe)2 to give [(mu-H)Ru3(CO)10(mu2-2,3-eta2-NNN(O)C6 H4)] and [(mu-H)Ru3(CO)9(mu2-eta1 : eta2-C5H4N(O)S)], respectively. Irradiation of these two new triruthenium metal clusters individually with 350 nm UV l...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2002)075<0457:ntcapd>2.0.co;2
更新日期:2002-05-01 00:00:00
abstract::The cellular uptake of Photofrin II (PII) was studied using fluorescence imaging and chemical extraction. The influence of serum and low density lipoproteins (LDL) was examined under a variety of experimental conditions employing cultured human cells of different origins as well as a subcutaneously SMT-F tumor implant...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1990.tb01702.x
更新日期:1990-02-01 00:00:00
abstract::The maximum erythemal dose rate (EDRmax ) at the Earth's surface tends to occur at local noon. However, clouds can make the timing of EDRmax significantly away from local noon. In fact, EDRmax and its time of occurrence depend mainly on the solar zenith angle at noon (SZAn ), site's altitude, the total ozone column (T...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13054
更新日期:2019-05-01 00:00:00
abstract::Supported silver nanoparticles (Ag NPs) were prepared by chemical reduction method with a sol-gel method. The structure, morphology, and interconnectivity of Ag/TiO2 nanocomposites (NCs) were analyzed using different instrumental techniques. Transmission electron microscopy reveals the Ag NPs have uniformly distribute...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12983
更新日期:2018-11-01 00:00:00
abstract::Molecular beacons (MB) are becoming more common as sequence-selective detectors of nucleic acids. Although they can easily detect single-base mismatches, they have never been used to directly detect DNA or RNA damage. To measure the degree of ultraviolet (UV) light damage in oligonucleotides, we report a novel MB appr...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-09-02-RA-301
更新日期:2005-03-01 00:00:00
abstract::The efficiency of ruthenium complexes for photosensitizing DNA damage depends on the oxidizing character of their ligands. Here we report on the difference in behavior of tris(2.2'-bipyrazyl)ruthenium(II) (Ru[bpz]3(2+)), tris(2,2'-bipyridyl)ruthenium(II) (Ru[bipy]3(2+)) and cis-dichlorobis (2,2'-bipyrazyl)ruthenium(II...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1997.tb01906.x
更新日期:1997-04-01 00:00:00
abstract::To clarify the role of the Golgi apparatus in photodynamic therapy-induced apoptosis, its signaling pathway was studied after photodynamic treatment of human cervix carcinoma cell line HeLa, in which a photosensitizer, 2,4,5,7-tetrabromorhodamine 123 bromide (TBR), was incorporated into the Golgi apparatus. Laser scan...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2003)078<0241:cacacb>2.0.co;2
更新日期:2003-09-01 00:00:00
abstract::In the present study, hypericin analogs with an increased hydrophilic character were synthesized. As chemical modifications alter the lipophilicity/hydrophilicity balance together with the photophysical/chemical background of the molecule the influence of these structural changes on the cellular uptake, retention and ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2001)074<0164:ivsotp>2.0.co;2
更新日期:2001-08-01 00:00:00
abstract::We investigated the effect of the cation-π interaction on the susceptibility of a tryptophan model system toward interaction with singlet oxygen, that is, type II photooxidation. The model system consists of two indole units linked to a lariat crown ether to measure the total rate of removal of singlet oxygen by the i...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13287
更新日期:2020-11-01 00:00:00