Feasibility of using fluoresceinyl Cypridina luciferin analog in a novel chemiluminescence method for real-time photodynamic therapy dosimetry.

Abstract:

:Singlet oxygen ((1)O(2)) is the most important cytotoxic agent in photodynamic therapy (PDT). The feasibility of using a chemiluminescence (CL) probe, 3,7-dihydro-6-[4-(2-(N'-(5-fluoresceinyl)thioureido)ethoxy)phenyl]-2-methylimidazo{1,2-a}pyrazin-3-one sodium salt (fluoresceinyl Cypridina luciferin analog, FCLA), to monitor (1)O(2) production during PDT is evaluated in vitro. Lymphoma cells were treated with various protocols of PDT. The results show that the FCLA-CL production during PDT is linearly related to the corresponding cytotoxicity, regardless of the treatment protocol. With minimum cytotoxicity and interference to the PDT treatment outcome, the FCLA-CL system is an effective means to quantify PDT (1)O(2) production and may provide an alternative real-time dosimeter.

journal_name

Photochem Photobiol

authors

Qin Y,Xing D,Luo S,Zhou J,Zhong X,Chen Q

doi

10.1562/2005-05-20-RA-536

subject

Has Abstract

pub_date

2005-11-01 00:00:00

pages

1534-8

issue

6

eissn

0031-8655

issn

1751-1097

pii

2005-05-20-RA-536

journal_volume

81

pub_type

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