Abstract:
:We study the fluorescence lifetime of the well-known 1-pyrene butyric acid (PBA) to assess oxygen concentrations in living cells. The behavior of the probe is first studied in water, ethanol, protein solution and liposome suspension. The Stern-Volmer plot of these solutions is linear, and the bimolecular reaction rate constant agrees with previous observations. In single living cells, the PBA lifetime decreases with oxygen concentration (185 to 55 ns). The probe lifetime differences between living cells and liposome suspension, especially under nitrogen atmosphere, suggest a supplemental pathway for the deactivation of the probe. We simplify further the complex living cells system by stopping the cell functions and studying freshly fixed cells. In this case, we obtained an increase of PBA lifetime under nitrogen atmosphere (215 ns).
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Ribou AC,Vigo J,Salmon JMdoi
10.1562/2004-03-11-RA-109subject
Has Abstractpub_date
2004-09-01 00:00:00pages
274-80issue
2eissn
0031-8655issn
1751-1097pii
2004-03-11-RA-109journal_volume
80pub_type
杂志文章abstract::Cellular autofluorescence spectra were monitored in a single-beam gradient force optical trap ("optical tweezers") in order to probe the physiological effects of near infrared and UVA (320-400 nm) microirradiation. Prior to trapping, Chinese hamster ovary cells exhibited weak UVA-excited autofluorescence with maxima a...
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journal_title:Photochemistry and photobiology
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pub_type: 杂志文章
doi:10.1111/j.1751-1097.1995.tb08624.x
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