Genome-wide examination of the natural solar radiation response in Shewanella oneidensis MR-1.

Abstract:

:We previously reported that Shewanella oneidensis MR-1 is extremely sensitive to natural solar radiation (NSR). Here we analyzed the global transcriptional profile of MR-1 during a 1-h recovering period after exposure to ambient solar light at a dose that yields about 20% survival rate on a Luria-Bertani (LB) plate. We observed the induction of DNA damage-repair genes, the SOS response as well as detoxification strategies that we previously observed in MR-1 following artificial UV-A irradiation. Few prophage-related genes were induced by natural solar UV radiation, however, in contrast to what was observed following artificial UV-B irradiation. Overall, the cellular response to NSR in MR-1 was more similar to that of UV-A than that of UV-B, but additional genes involved in detoxification were induced compared with induction by either UV-B or UV-A or their sum. Thus, oxidative stress appeared to contribute greatly to the NSR-induced cytotoxic effects in MR-1. A total of 29.1% of genome showed differential expression following NSR exposure, which is much greater than following exposure by UV-B (4.0%), UV-A (8.2%) or their sum (10.7%). Our data suggest that NSR may impact biological processes in a much more complex manner than previously thought.

journal_name

Photochem Photobiol

authors

Qiu X,Tiedje JM,Sundin GW

doi

10.1562/2005-04-15-RA-490

subject

Has Abstract

pub_date

2005-11-01 00:00:00

pages

1559-68

issue

6

eissn

0031-8655

issn

1751-1097

pii

2005-04-15-RA-490

journal_volume

81

pub_type

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