Abstract:
:SV40 DNA was irradiated in vitro and in vivo with UV-C (240-280 nm) and UV-B (280-320 nm) light, and damaged sites sensitive to digestion with Escherichia coli endonuclease III (endo III) and bacteriophage T4 endonuclease V (endo V) were quantified. The frequency of endo III-sensitive sites (primarily cytosine photohydrates) induced was 1-2% of the frequency of endo V-sensitive sites (cyclobutane dimers) in both purified SV40 DNA and intracellular episomal SV40 DNA. Endo III- and endo V-sensitive sites in DNA were induced in the same relative proportion at both UV-C and UV-B wavelengths. We found no evidence to support earlier inferences that intracellular conditions enhance the formation of cytosine photohydrates or other monobasic forms of DNA damage.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Mitchell DL,Jen J,Cleaver JEdoi
10.1111/j.1751-1097.1991.tb02084.xsubject
Has Abstractpub_date
1991-11-01 00:00:00pages
741-6issue
5eissn
0031-8655issn
1751-1097journal_volume
54pub_type
杂志文章abstract::An ongoing study in our laboratories is to examine the relationship of DNA repair defects to human cancer. Our underlying hypothesis has been that human tumors may arise that lack interesting DNA repair pathways if these pathways are important in preventing cancer. In this study, we found that the UV-irradiated adenov...
journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1991.tb03638.x
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00345.x
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2009.00570.x
更新日期:2009-09-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00359.x
更新日期:2008-09-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
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pub_type: 杂志文章
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更新日期:1990-05-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::We previously reported that Shewanella oneidensis MR-1 is extremely sensitive to natural solar radiation (NSR). Here we analyzed the global transcriptional profile of MR-1 during a 1-h recovering period after exposure to ambient solar light at a dose that yields about 20% survival rate on a Luria-Bertani (LB) plate. W...
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1991.tb08470.x
更新日期:1991-01-01 00:00:00
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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abstract::The objective of the present study was to investigate the treatment of 9L gliosarcoma brain tumor in the rat with the combination of surgical resection and photodynamic therapy (PDT). Nude rats with intracranial 7-day-old 9L gliomas were randomly subjected to no treatment, PDT alone (Photofrin: 2 mg kg(-1), optical: 8...
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