Abstract：
:There are controversial reports in the literature concerning the reactivity of singlet oxygen ((1)O(2)) with the redox probe 2',7'-dichlorodihydrofluorescein (DCFH). By carefully preparing solutions in which (1)O(2) is quantitatively generated in the presence of DCFH, we were able to show that the formation rate of the fluorescent molecule derived from DCFH oxidation, which is 2',7'-dichlorofluorescein (DCF), increases in D(2)O and decreases in sodium azide, proving the direct role of (1)O(2) in this process. We have also prepared solutions in which either (1)O(2) or dication (MB(2+)) and semi-reduced (MB) radicals of the sensitizer and subsequently super-oxide radical (O(2)(-)) are generated. The absence of any effect of SOD and catalase ruled out the DCFH oxidation by O(2)(-), indicating that both (1)O(2) and MB(2+) react with DCFH. Although the formation of DCF was 1 order of magnitude larger in the presence of MB(2+) than in the presence of (1)O(2), considering the rate of spontaneous decays of these species in aqueous solution, we were able to conclude that the reactivity of (1)O(2) with DCFH is actually larger than that of MB(2+). We conclude that DCFH can continue to be used as a probe to monitor general redox misbalance induced in biologic systems by oxidizing radicals and (1)O(2).
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Daghastanli NA,Itri R,Baptista MSdoi
10.1111/j.1751-1097.2008.00345.xsubject
Has Abstractpub_date
2008-09-01 00:00:00pages
1238-43issue
5eissn
0031-8655issn
1751-1097pii
PHP345journal_volume
84pub_type
杂志文章abstract：:Schinus terebinthifolius is a plant rich in phenolic compounds, which have antioxidant properties and can provide new opportunities for treatment and prevention of diseases mediated by ultraviolet radiation like photoaging and skin cancer. The aim of this study was to evaluate the photoprotective potential and ex vivo...
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abstract：:All-trans retinoic acid (ATRA) plays essential roles in the normal biological processes and the treatment of cancer and skin diseases. Considering its photosensitive property, many studies have been focused on the photochemistry of ATRA. In this study, we investigated the transient phenomena in the laser flash photoly...
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journal_title：Photochemistry and photobiology
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pub_type： 杂志文章
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更新日期：1989-12-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2014-11-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
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更新日期：2015-11-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 评论,杂志文章
doi：10.1111/php.13102
更新日期：2019-09-01 00:00:00
