Abstract：
:By a technique which combines rapid mixing of cells and hematoporphyrin (HP) with a short duration of illumination, the photodynamic inactivation of yeast cells was investigated, particularly, in seeking for the information of the location of HP at the time of action. The fluence-survival curves obtained under the conditions where the reaction mixture was kept in the dark for 1 s, 60 s and even 35 min before illumination were indistinguishable from each other, indicating no interaction between cells and sensitizers took place in about 30 min in such a way that the photodynamic efficiency could be modified. It is unlikely tha HP acted intracellularly, since the protective effect of N-3 was observed at concentrations as low as 0.5 mM. Furthermore, the rate constant kp related to the protective effect of N-3 was estimated to be 1 x 10(8) M(-1)s(-1) under the assumption that (1) O2 was the active intermediate and had a lifetime of 12 microseconds under the present conditions. This value of kp is rather close to that of kq' the quenching rate constant of N-3 for (1)O2, of which the accepted value is 2 x 10(8) M(-1)s(-1) in the homogeneous aqueous system. This information, together with the absence of uptake of HP by cells and a well response of survival upon illumination to the D2O fraction of the reaction mixture, provide strong bases for the argument that direct interaction of HP with yeast cells is of minor importance in the photodynamic processes, and the photodynamic action is largely mediated by an intermediated ((1)O2) generated in bulk medium.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Ito Tsubject
Has Abstractpub_date
1981-10-01 00:00:00pages
521-4issue
4eissn
0031-8655issn
1751-1097journal_volume
34pub_type
杂志文章abstract：:We study the fluorescence lifetime of the well-known 1-pyrene butyric acid (PBA) to assess oxygen concentrations in living cells. The behavior of the probe is first studied in water, ethanol, protein solution and liposome suspension. The Stern-Volmer plot of these solutions is linear, and the bimolecular reaction rate...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-03-11-RA-109
更新日期：2004-09-01 00:00:00
abstract：:Betanin (betanidin 5-O-β-D-glucoside) is a water-soluble plant pigment used as a color additive in food, drugs and cosmetic products. Despite its sensitivity to light and heat, betanin maintains appreciable tinctorial strength in low acidic and neutral conditions, where the color of other plant pigments, such as antho...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12897
更新日期：2018-09-01 00:00:00
abstract：:Quantum yields for the formation of superoxide ions, O2-., and singlet oxygen, 1O2, were determined during the photolyses of gilvocarcin M (GM) in air-saturated dry dimethylsulfoxide (DMSO) and in 45:55 (vol/vol) DMSO-water mixtures. The quantum yield for the photoreduction of methyl viologen by GM in nitrogen-saturat...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb02361.x
更新日期：1995-09-01 00:00:00
abstract：:Herein, we present a comparative study about the photoinactivation of Staphylococcus aureus (Gram-positive model) and Escherichia coli (Gram-negative model) employing a neutral and a dicationic axially functionalized SiIV -phthalocyanine. Depending on the charge of the siloxane moiety (neutral monosaccharide or cation...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12881
更新日期：2018-09-01 00:00:00
abstract：:The interaction of photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) and hyperthermia is not well understood. In the present study, significant enhancement of tumor damage was observed after simultaneous application of ALA-PDT and IR-induced hyperthermia using a broad-band incoherent light source. One hour a...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：:The Photochemical Research Group from San Luis (Argentina) highlights the contributions of Dr. Norman Andino García to the development of the group as a way to show him our gratitude for all his support. ...
journal_title：Photochemistry and photobiology
pub_type： 社论
doi：10.1111/php.12988
更新日期：2018-11-01 00:00:00
abstract：:Protoporphyrin IX dimethyl ester (PME), a dimethyl esterification of protoporphyrin IX (PpIX), exhibits higher intracellular uptake into NPC/CNE2 cells, a poorly differentiated human nasopharyngeal carcinoma, than does PpIX. Phototoxicity studies reveal PME to be a more potent photosensitizer than is PpIX, at the earl...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)076<0678:acopia>2.0.co;2
更新日期：2002-12-01 00:00:00
abstract：:A new water-soluble porphyrin derivative, 2,4-bis(1-decyloxyethyl)-deuteroporphyrinyl-6,7-bisaspart ic acid (C10-DP), and its metal complexes (Ga, I(n), Zn, Mn, Cu, Ni and Fe) were examined for their physicochemical properties (absorption, fluorescence, triplet lifetime and partition coefficient) and photocytotoxicity...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb02928.x
更新日期：1993-04-01 00:00:00
abstract：:Pharmacological therapy is widely used in the treatment of muscle injuries. On the other hand, low-level laser therapy (LLLT) arises as a promising nonpharmacological treatment. The aim of this study was to analyze the effects of sodium diclofenac (topical application) and LLLT on morphological aspects and gene expres...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2012.01232.x
更新日期：2013-03-01 00:00:00
abstract：:We report the spectroscopic properties of fluorescein, x-rhodamine, tetramethyl-rhodamine, attached to single strand, duplex DNA, and to the digestion products by DNAse I. The properties reported include: molar absorptivity, quantum yield, absorbance and fluorescence spectra, fluorescence lifetime, intrinsic lifetime ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2009.00663.x
更新日期：2010-03-01 00:00:00
abstract：:Photodynamic therapy (PDT) uses laser light to activate a photosensitizer that has been absorbed preferentially by cancer cells after systemic administration. A phototoxic reaction ensues resulting in cell death and tissue necrosis. Some cells, however, may survive PDT. This study was performed to determine if survivi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:Proteases like urokinase-type plasminogen activator (uPA) play an important role in tumor invasion. Cells derived from ultraviolet radiation (UVR)-induced corneal sarcomas of Monodelphis domestica produce relatively high levels of uPA compared to the untransformed keratocytes suggesting a mechanism for their invasiven...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2001)073<0318:uaicfm>2.0.co;2
更新日期：2001-03-01 00:00:00
abstract：:We previously reported that a low level of lysosomal photodamage potentiated the phototoxic effect of subsequent mitochondrial photodamage mediated by the benzoporphyrin derivative (BPD) in murine hepatoma 1c1c7 cells. This was attributed to release of Ca2+ from damaged lysosomes and a calpain-mediated conversion of t...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12805
更新日期：2017-11-01 00:00:00
abstract：:A novel antibody-fluorescence method has been developed to elucidate the chromophore topography in phytochrome as it undergoes a photochromic transformation. Förster energy transfer from N-terminal bound, fluorescently labeled Oat-25 Fab antibody fragments to the phytochrome chromophore was measured. The results sugge...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1992.tb02227.x
更新日期：1992-11-01 00:00:00
abstract：:Two-photon laser scanning microscopy (TPLSM) of endogenous reduced nicotinamide adenine dinucleotide (NAD(P)H) provides important information regarding the cellular metabolic state. When imaging the punctate mitochondrial fluorescence originating from NAD(P)H in a rat basophilic leukemia (RBL) cell at low laser powers...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-08-05-RA-263
更新日期：2005-03-01 00:00:00
abstract：:Flash-induced absorbance spectroscopy was used to analyze the proton uptake and electron transfer properties of photosynthetic reaction centers (RC) of Rhodobacter capsulatus that have been genetically modified near the primary quinone electron acceptor (Q(A)). M246Ala and M247Ala, which are symmetry-related to the po...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)075<0126:puorcr>2.0.co;2
更新日期：2002-02-01 00:00:00
abstract：:A solution of iodouracil (0.2 mg/mL) and KI (0.1 M) in 0.23 M borate buffer (pH 7) forms triiodide upon exposure to UVB. In the presence of thyodene, the blue starch-iodine complex is formed, the intensity of which is proportional to the amount of UVB exposure. Studies were conducted using either a sunlamp or solar ir...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-08-01 00:00:00
abstract：:The capacity of some linear and angular furocoumarins to induce DNA-protein cross-links by UVA (320-400 nm) irradiation has been evaluated in Chinese hamster ovary cells. Two linear furocoumarins, psoralen and 8-methoxypsoralen appeared to be capable of inducing DNA-protein cross-links to a noticeable extent. 4'-Methy...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb04914.x
更新日期：1993-07-01 00:00:00
abstract：:Under continuous photolysis at 675 nm, liposomal zinc phthalocyanine associated with nitrosyl ruthenium complex [Ru(NH.NHq)(tpy)NO](3+) showed the detection and quantification of nitric oxide (NO) and singlet oxygen ((1)O(2)) release. Photophysical and photochemical results demonstrated that the interaction between th...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2008.00481.x
更新日期：2009-05-01 00:00:00
abstract：:It is generally assumed that a central metal is essential for the efficiency of phthalocyanines in photodynamic therapy (PDT) of cancer. Contrary to the set opinion, the results of the present study indicate that the metal-free sulfonated phthalocyanines (H2PcSn, where n is the number of sulfonate groups per molecule)...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)075<0527:cwmine>2.0.co;2
更新日期：2002-05-01 00:00:00
abstract：:Latia neritoides is a small limpet-like snail that produces a bright green bioluminescence (BL) via a unique light-emitting system. The process, mechanism, and even light emitter of its light emission remain unknown, although this BL has been known for decades. Unlike the other BL systems, neither the luciferin (Luc) ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12876
更新日期：2018-05-01 00:00:00
abstract：:Iranian firefly larvae, Lampyris turkestanicus collected from north of Iran and their luciferin were analyzed by HPLC, TLC, MS and spectroscopic Fourier transform ([FT]-IR, FT-NMR, UV and fluorescence) methods. The results showed that luciferin in L turkestanicus lanterns was the same as in the American firefly, Photi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1996.tb01841.x
更新日期：1996-11-01 00:00:00
abstract：:In the present work, we study the photodynamic action of cercosporin (cerco), a naturally occurring photosensitizer, on human cancer multicellular spheroids. U87 spheroids exhibit double the uptake of cerco than T47D and T98G spheroids as shown by flow cytometry on the single cell level. Moreover, cerco is efficiently...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13257
更新日期：2020-05-01 00:00:00
abstract：:The UVA and UVB components of sunlight can produce three classes of bipyrimidine DNA photolesions [cyclobutane pyrimidine dimers (CPDs), pyrimidine (6-4) pyrimidone photoproducts (6-4PPs) and related Dewar valence isomers (DewarPPs)]. The UVA/UVB ratio of sunlight is high in winter and low in summer in the Northern He...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13361
更新日期：2020-12-03 00:00:00
abstract：:Previous studies have shown that natural killer (NK) cell activity was suppressed in volunteer subjects exposed to ultraviolet radiation (UVR) from solarium lamps. The present studies were carried out to determine the spectrum of UVR responsible for suppression of NK activity and to develop in vitro methods to analyze...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb02287.x
更新日期：1993-02-01 00:00:00
abstract：:The photochemistry of caged compounds of the o-nitrobenzyl type has been investigated thoroughly in the past. However, even recently new side reactions have been discovered. Earlier, we reported [Bley, F., K. Schaper, and H. Görner (2008), Photochem. Photobiol.84 162-171] that we found long-lived triplet states which ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2009.00560.x
更新日期：2009-09-01 00:00:00
abstract：:Aqueous solutions of KI were examined for use as chemical actinometers to measure 254 nm (germicidal) radiation. Irradiation results in electron ejection from iodide such that aqueous electrons and iodine atoms are formed. In the presence of N2O, an electron scavenger, recombination of these two reactive species is el...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb04986.x
更新日期：1993-12-01 00:00:00
abstract：:Cyclooxygenase (COX)-2 inhibitors are known to be used as chemopreventative agents against certain malignancies. Thus far, there has been very limited information on whether COX-2 inhibitors protect against chronic narrow-band UVB (NB-UVB)-induced immunosuppression. The present study investigated the effect of nonsele...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00289.x
更新日期：2008-03-01 00:00:00
abstract：:From June to September 2005, we carried out experiments to determine the ultraviolet radiation (UVR) -induced photoinhibition of summer phytoplankton assemblages from a coastal site of the South China Sea. Variability in taxonomic composition was determined throughout the summer, with a peak chlorophyll a (chl a appro...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00154.x
更新日期：2007-07-01 00:00:00
abstract：:A 2-year field study on the effect of different light environments, obtained by using cladding materials (polyethylene films and shade net) able to cut off specific regions of the photosynthetically active radiation and ultraviolet wavebands, on the growth and phenylpropanoids content of Achillea collina grown in the ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12150
更新日期：2014-01-01 00:00:00
