Photodynamic Efficacy of Cercosporin in 3D Tumor Cell Cultures.

Abstract:

:In the present work, we study the photodynamic action of cercosporin (cerco), a naturally occurring photosensitizer, on human cancer multicellular spheroids. U87 spheroids exhibit double the uptake of cerco than T47D and T98G spheroids as shown by flow cytometry on the single cell level. Moreover, cerco is efficiently internalized by cells throughout the spheroid as shown by confocal microscopy, for all three cell lines. Despite their higher cerco uptake, U87 spheroids show the least vulnerability to cerco-PDT, in contrast to the other two cell lines (T47D and T98G). While 300 μm diameter spheroids consistently shrink and become necrotic after cerco PDT, bigger spheroids (>500 μm) start to regrow following blue-light PDT and exhibit high viability. Cerco-PDT was found to be effective on bigger spheroids reaching 1mm in diameter especially under longer exposure to yellow light (~590 nm). In terms of metabolism, T47D and T98G undergo a complete bioenergetic collapse (respiration and glycolysis) as a result of cerco-PDT. U87 spheroids also experienced a respiratory collapse following cerco-PDT, but retained half their glycolytic activity.

journal_name

Photochem Photobiol

authors

Grigalavicius M,Mastrangelopoulou M,Arous D,Juzeniene A,Ménard M,Skarpen E,Berg K,Theodossiou TA

doi

10.1111/php.13257

subject

Has Abstract

pub_date

2020-05-01 00:00:00

pages

699-707

issue

3

eissn

0031-8655

issn

1751-1097

journal_volume

96

pub_type

杂志文章
  • Dependence of fibroblast autofluorescence properties on normal and transformed conditions. Role of the metabolic activity.

    abstract::The dependence of autofluorescence properties on the metabolic and functional engagement and on the transformation condition was studied on single cells. Normal Galliera rat fibroblasts at low subculture passage (cell strain), at high subculture passage (stabilized cell line), and transformed cell line derived from a ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(1999)069<0364:dofapo>2.3.co;2

    authors: Croce AC,Spano A,Locatelli D,Barni S,Sciola L,Bottiroli G

    更新日期:1999-03-01 00:00:00

  • Indication for a radical intermediate preceding the signaling state in the LOV domain photocycle.

    abstract::The blue light photoreceptor phototropin mediates crucial processes in plants leading to optimization of photosynthesis. Phototropin comprises two flavin mononucleotide-binding LOV (light-, oxygen-, or voltage-sensitive) domains. The LOV domains undergo a photocycle upon illumination, in which two intermediates have b...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2011.00901.x

    authors: Bauer C,Rabl CR,Heberle J,Kottke T

    更新日期:2011-05-01 00:00:00

  • Synthesis and spectroscopic characterization of photo-affinity peptide ligands to study rhodopsin-G protein interaction.

    abstract::G protein-coupled receptors (GPCRs) are involved in the control of virtually all aspects of our behavior and physiology. Activated receptors catalyze nucleotide exchange in heterotrimeric G proteins (composed of alpha.GDP, beta and gamma subunits) on the inner surface of the cell membrane. The GPCR rhodopsin and the G...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2008.00304.x

    authors: Chen Y,Herrmann R,Fishkin N,Henklein P,Nakanishi K,Ernst OP

    更新日期:2008-07-01 00:00:00

  • Activities of the bimodal fluorescent protein produced by Photobacterium phosphoreum strain bmFP in the luciferase reaction in vitro.

    abstract::The activity of the bimodal fluorescent protein (bmFP) (lambda max, 488 and 517 nm) in the in vitro luciferase reaction has been studied. The bmFP that is produced by Photobacterium phosphoreum strain bmFP is a dimer of two homologous subunits binding four riboflavin 5'-phosphate (FMN)-myristate chromophores. The addi...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2000)071<0237:aotbfp>2.0.co;2

    authors: Karatani H,Konaka T

    更新日期:2000-02-01 00:00:00

  • Photosensitized oxidation and inactivation of pyocyanin, a virulence factor of Pseudomonas aeruginosa.

    abstract::Pyocyanin (PyO-) (1-hydroxy-5-methylphenazine) is a cytotoxic compound secreted by Pseudomonas aeruginosa, an omnipresent bacterium and a human pathogen. We report that visible light illumination in the presence of rose bengal, or riboflavin, in aerated solutions (pH 7.0-7.2) induces irreversible loss of the pigment's...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2005-07-29-RA-626

    authors: Reszka KJ,Denning GM,Britigan BE

    更新日期:2006-03-01 00:00:00

  • Hypericin-induced phototoxicity in cultured fibroblasts and swine erythrocytes.

    abstract::Hypericin is a naturally occurring photosensitizer, whose presence in plants has been responsible for cutaneous phototoxicity in grazing animals. The photosensitizing properties of this agent have recently been exploited in models for anti-tumor and anti-viral activity. The cytotoxicity of hypericin and light was asse...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1996.tb02438.x

    authors: Yu H,Wolford ST,Kegode R,Zhao W,Osweller GD

    更新日期:1996-07-01 00:00:00

  • The photochemistry of the retinoids as studied by steady-state and pulsed methods.

    abstract::The retina and retinal pigment epithelium contain a number of retinoids in a metabolic pathway that eventually forms the visual pigments. This study investigates the photochemistry of those retinoids that may contribute to light-induced damage to the retina. These include retinal (RAL), retinol (ROL), retinylpalmitate...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1996.tb05673.x

    authors: Dillon J,Gaillard ER,Bilski P,Chignell CF,Reszka KJ

    更新日期:1996-05-01 00:00:00

  • Triplet Excited States and Singlet Oxygen Production by Analogs of Red Wine Pyranoanthocyanins.

    abstract::During the maturation of red wines, the anthocyanins of grapes are transformed into pyranoanthocyanins, which possess a pyranoflavylium cation as their basic chromophore. Photophysical properties of the singlet and triplet excited states of a series of synthetic pyranoflavylium cations were determined at room temperat...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12973

    authors: Silva GTM,Thomas SS,Silva CP,Schlothauer JC,Baptista MS,Freitas AA,Bohne C,Quina FH

    更新日期:2019-01-01 00:00:00

  • Pomegranate fruit extract modulates UV-B-mediated phosphorylation of mitogen-activated protein kinases and activation of nuclear factor kappa B in normal human epidermal keratinocytes paragraph sign.

    abstract::Excessive exposure of solar ultraviolet (UV) radiation, particularly its UV-B component, to humans causes many adverse effects that include erythema, hyperplasia, hyperpigmentation, immunosuppression, photoaging and skin cancer. In recent years, there is increasing use of botanical agents in skin care products. Pomegr...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2004-08-06-RA-264

    authors: Afaq F,Malik A,Syed D,Maes D,Matsui MS,Mukhtar H

    更新日期:2005-01-01 00:00:00

  • UVB radiation affects growth, reproduction and tissue structure of Daphnia magna across several temperatures.

    abstract::We examined the effects of daily exposure to UVB on growth, reproduction and histological characteristics of Daphnia magna over two generations at 20, 22, 25 and 30°C. Animals were exposed to 16 h of UVA and photosynthetically active radiation daily. Treated animals received 6 h of UVB during the light phase. Parental...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2012.01197.x

    authors: Huebner JD,Loadman NL,Wiegand MD,Huebner E,Palitsky DJ,Husarewycz WH

    更新日期:2013-01-01 00:00:00

  • Low-level laser therapy and sodium diclofenac in acute inflammatory response induced by skeletal muscle trauma: effects in muscle morphology and mRNA gene expression of inflammatory markers.

    abstract::Pharmacological therapy is widely used in the treatment of muscle injuries. On the other hand, low-level laser therapy (LLLT) arises as a promising nonpharmacological treatment. The aim of this study was to analyze the effects of sodium diclofenac (topical application) and LLLT on morphological aspects and gene expres...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2012.01232.x

    authors: de Almeida P,Lopes-Martins RÁ,Tomazoni SS,Albuquerque-Pontes GM,Santos LA,Vanin AA,Frigo L,Vieira RP,Albertini R,de Carvalho Pde T,Leal-Junior EC

    更新日期:2013-03-01 00:00:00

  • Effect of preillumination on photomotile responses of the marine ciliate Fabrea salina.

    abstract::Fabrea salina is a marine ciliate that shows photomotile responses such as positive phototaxis and a step-down photophobic reaction. We found that preilluminated F. salina cells show a phototactic response significantly greater than that of dark-adapted cells when exposed to the same phototactic light stimulus. In par...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2003)077<0204:eopopr>2.0.co;2

    authors: Marangoni R,Colombetti G

    更新日期:2003-02-01 00:00:00

  • "Hot spots" associated with the photoinduced binding of cis-dichloro bis(1,10 phenanthroline)rhodium(III) chloride to HIV-1 and c-raf DNA.

    abstract::The octahedral rhodium complex, cis-dichloro bis(1,10 phenanthroline)rhodium(III) chloride (BISPHEN), is known to form covalent linkages with DNA involving the attachment of the metal to a base. In order to determine the sequence selectivity of this chemistry, solutions of the complex containing one of the double-stra...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2000)072<0731:hsawtp>2.0.co;2

    authors: Morrison H,Harmon H

    更新日期:2000-12-01 00:00:00

  • The effects of low density lipoproteins on uptake of Photofrin II.

    abstract::The cellular uptake of Photofrin II (PII) was studied using fluorescence imaging and chemical extraction. The influence of serum and low density lipoproteins (LDL) was examined under a variety of experimental conditions employing cultured human cells of different origins as well as a subcutaneously SMT-F tumor implant...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1990.tb01702.x

    authors: Korbelik M,Hung J,Lam S,Palcic B

    更新日期:1990-02-01 00:00:00

  • Photodynamic therapy with photofrin II induces programmed cell death in carcinoma cell lines.

    abstract::The mode of cell death following photodynamic therapy was investigated from the perspective of programmed cell death or apoptosis. Human prostate carcinoma cells (PC3), human non-small cell lung carcinoma (H322a) and rat mammary carcinoma (MTF7) were treated by photodynamic therapy. An examination of extracted cellula...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1994.tb05066.x

    authors: He XY,Sikes RA,Thomsen S,Chung LW,Jacques SL

    更新日期:1994-04-01 00:00:00

  • Photochemistry of DNA using 193 nm excimer laser radiation.

    abstract::Photoproducts in double-stranded DNA induced by 193 nm radiation have been investigated. Double-stranded, supercoiled pBR322 DNA in buffered aqueous solution was exposed to varying fluences of 193 nm radiation from an ArF excimer laser. The quantum yields for formation of cyclobutylpyrimidine dimers, frank strand brea...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1990.tb01961.x

    authors: Kochevar IE,Buckley LA

    更新日期:1990-05-01 00:00:00

  • Type I and Type II Photosensitized Oxidation Reactions: Guidelines and Mechanistic Pathways.

    abstract::Here, 10 guidelines are presented for a standardized definition of type I and type II photosensitized oxidation reactions. Because of varied notions of reactions mediated by photosensitizers, a checklist of recommendations is provided for their definitions. Type I and type II photoreactions are oxygen-dependent and in...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/php.12716

    authors: Baptista MS,Cadet J,Di Mascio P,Ghogare AA,Greer A,Hamblin MR,Lorente C,Nunez SC,Ribeiro MS,Thomas AH,Vignoni M,Yoshimura TM

    更新日期:2017-07-01 00:00:00

  • Sunscreens and Cosmetic Formulations Containing Ascorbyl Tetraisopalmitate and Rice Peptides for the Improvement of Skin Photoaging: a Double-blind, Randomized Placebo-controlled Clinical Study.

    abstract::Photoprotective formulations containing substances with antioxidant properties in combination have been used as a strategy for the improvement of photoaged skin conditions. However, there is a lack of studies evaluating the clinical efficacy of these substances in young women with signs of photoaging. Thus, the object...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13390

    authors: Fossa Shirata MM,Maia Campos PMBG

    更新日期:2021-02-02 00:00:00

  • Reactions of excited states of phenoxazin-3-one dyes with amino acids.

    abstract::The interaction with amino acids of the excited states of the N-oxide resazurin and its deoxygenation product resorufin, has been studied in aqueous solution at pH 7.5. Steady-state and time-resolved studies show that the fluorescence is quenched by amino acids. Complexation of the dyes in the ground state with aromat...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2004-10-12-RA-342

    authors: Villegas ML,Bertolotti SG,Previtali CM,Encinas MV

    更新日期:2005-07-01 00:00:00

  • Optical properties of fluorescein-labeled organoclay.

    abstract::We report the preparation of aminopropyl-functionalized magnesium phyllosilicate (Mg-organoclay) conjugated to fluorescein isothiocyanate (FITC) functionality (FITC-organoclay) by one-pot sol-gel synthesis. The physical characteristics of the Mg- and FITC-organoclays were examined by scanning electron microscopy (SEM)...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2009.00701.x

    authors: Lee YC,Lee TH,Han HK,Go WJ,Yang JW,Shin HJ

    更新日期:2010-05-01 00:00:00

  • Photooxidation products of merocyanine 540 formed under preactivation conditions for tumor therapy.

    abstract::In order to gain insight into the preactivation of merocyanine 540 (MC540) 1 for the photodynamic therapy (Gulliya et al., 1990a, Photochem. Photobiol. 52, 831-838) its photo-oxidation was investigated. After irradiation of MC540 1 on a preparative scale three main photodegradation products were isolated with 16-20% y...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1992.tb02157.x

    authors: Franck B,Schneider U

    更新日期:1992-08-01 00:00:00

  • On the Formation of Thymine Photodimers in Thymine Single Strands and Calf Thymus DNA.

    abstract::Solar light leads to thymine dimers that are mutagenic and primary cause of skin cancer. Here, we report absorption and synchrotron radiation circular dichroism (CD) spectra of Tn single strands with different number n of bases (n = 2-7, 10, 11) recorded after various 254 nm irradiation times. From a principal compone...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12183

    authors: Baggesen LM,Hoffmann SV,Nielsen SB

    更新日期:2014-01-01 00:00:00

  • Relationship between skin color and sun exposure history: a statistical classification approach.

    abstract::In this study our aim was to determine the biophysical values of constitutive skin color in Caucasians and to define the correlation between skin color and phototype assessed according to the Fitzpatrick method. Constitutive skin color was measured on the buttock, with a Minolta CR-200 colorimeter, in a population-of ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1997.tb08569.x

    authors: Rubegni P,Cevenini G,Flori ML,Fimiani M,Stanghellini E,Molinu A,Barbini P,Andreassi L

    更新日期:1997-02-01 00:00:00

  • Thermal recovery of iodopsin from photobleaching intermediates.

    abstract::The chloride effect on the photobleaching process of iodopsin, a chicken red-sensitive cone visual pigment, was studied in detail by time-resolved low-temperature spectroscopy at -40 degrees C to -10 degrees C. Decay-associated difference spectra obtained by kinetic analysis using the singular value decomposition meth...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2008.00332.x

    authors: Imamoto Y,Shichida Y

    更新日期:2008-07-01 00:00:00

  • Why is rose bengal more phototoxic to fibroblasts in vitro than in vivo?

    abstract::Photosensitized protein cross-linking has been recently developed to seal wounds and strengthen tissue. Although the photosensitizing dye, Rose Bengal (RB), is phototoxic to cultured cells, cytotoxicity does not accompany RB-photosensitized tissue repair in vivo. We investigated whether the environment surrounding cel...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12215

    authors: Yao M,Gu C,Doyle FJ Jr,Zhu H,Redmond RW,Kochevar IE

    更新日期:2014-03-01 00:00:00

  • Photosensitization by Photofrin II delivered to WI26VA4 SV40-transformed human fibroblasts by low density lipoproteins: inhibition of lipid synthesis and fatty acid uptake.

    abstract::Irradiation with 365 nm light of Wi26VA4 SV40-transformed human fibroblasts cultured for 24 h in the presence of low density lipoproteins loaded with the anticancer porphyrin mixture Photofrin II resulted in a near complete inhibition of [14C]oleic acid incorporation into triacylglycerols, cholesteryl esters and phosp...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1992.tb04209.x

    authors: Biade S,Mazière JC,Mora L,Santus R,Morlière P,Mazière C,Salmon S,Gatt S,Dubertret L

    更新日期:1992-01-01 00:00:00

  • Photoproducts formed in the photoyellowing of collagen in the presence of a fluorescent whitening agent.

    abstract::Fibrous proteins discolor on exposure to the UV component of sunlight. This effect is exacerbated in the presence of fluorescent whitening agents (FWAs), which are often applied to textiles to improve product brightness. Tryptophan photoproducts have been identified as significant contributors to protein photoyellowin...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2009.00614.x

    authors: Dyer JM,Clerens S,Cornellison CD,Murphy CJ,Maurdev G,Millington KR

    更新日期:2009-11-01 00:00:00

  • Photointeraction of benzophenone triplet with lysozyme.

    abstract::The quenching of the benzophenone triplet by lysozyme and its constituent amino acids in aqueous solutions have been studied. Native lysozyme quenches the benzophenone triplet with a high rate constant, 4 x 10(9) M-1 s-1. The quenching process takes place with production of significant amounts of free ketyl radicals, ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1989.tb08424.x

    authors: Encinas MV,Lissi EA,Vasquez M,Olea AF,Silva E

    更新日期:1989-05-01 00:00:00

  • Photodynamic therapy with topical delta-aminolevulinic acid delays UV photocarcinogenesis in hairless mice.

    abstract::Photodynamic therapy (PDT) with topical application of delta-aminolevulinic acid (ALA) followed by irradiation with visible light (ALA-PDT) is a relatively new and promising experimental treatment of superficial premalignant and malignant skin neoplasms. The purpose of this study was to determine whether ALA-PDT can p...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1997.tb03178.x

    authors: Stender IM,Bech-Thomsen N,Poulsen T,Wulf HC

    更新日期:1997-10-01 00:00:00

  • Facile Fabrication of Porous ZnS and ZnO Films by Coaxial Electrospinning for Highly Efficient Photodegradation of Organic Dyes.

    abstract::Porous ZnS and ZnO nano-crystal films were fabricated via a three-step procedure. First, Zn(CH3 COO)2 /Silk Fibroin nanofiber mats were prepared by coaxial electrospinning. Second, Zn(CH3 COO)2 /Silk Fibroin mats were immersed in NaS solution to react with S2- to obtain ZnS/Silk Fibroin nanofiber mats. Finally, ZnO po...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12836

    authors: Wang H,Liang L,Cheng X,Luo Y,Sun S

    更新日期:2018-01-01 00:00:00