Abstract：
:The blue light photoreceptor phototropin mediates crucial processes in plants leading to optimization of photosynthesis. Phototropin comprises two flavin mononucleotide-binding LOV (light-, oxygen-, or voltage-sensitive) domains. The LOV domains undergo a photocycle upon illumination, in which two intermediates have been detected by UV/Vis spectroscopy. The triplet excited state of flavin is formed and decays within a few microseconds into a photoadduct with an adjacent cysteine, which represents the signaling state of the LOV domain. For bond formation of the photoadduct, several reaction pathways have been proposed, but evidence for an intermediate at ambient conditions has not been found. Here, we performed nanosecond time-resolved UV/Vis spectroscopy on the phototropin-LOV1 domain from Chlamydomonas reinhardtii. We designed a flow cell which was used to efficiently replace the sample after each photoexcitation because the cycling time is in the order of hundreds of seconds. The comparison of difference spectra of the wild type with those of the C57S mutant that produces only the triplet excited state revealed the existence of an additional intermediate between the triplet and the adduct state. This intermediate exhibits spectral properties similar to a neutral flavin radical. This finding supports a reaction mechanism involving a neutral radical pair.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Bauer C,Rabl CR,Heberle J,Kottke Tdoi
10.1111/j.1751-1097.2011.00901.xsubject
Has Abstractpub_date
2011-05-01 00:00:00pages
548-53issue
3eissn
0031-8655issn
1751-1097journal_volume
87pub_type
杂志文章abstract：:Aqueous solutions of KI were examined for use as chemical actinometers to measure 254 nm (germicidal) radiation. Irradiation results in electron ejection from iodide such that aqueous electrons and iodine atoms are formed. In the presence of N2O, an electron scavenger, recombination of these two reactive species is el...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb04986.x
更新日期：1993-12-01 00:00:00
abstract：:The broad-band normal incidence UVB beam radiation has been measured at Neve Zohar, Dead Sea basin, using a prototype tracking instrument composed of a Model 501A UV-Biometer mounted on an Eppley Solar Tracker Model St-1. The diffuse and beam fraction of the solar global UVB radiation have been determined using the co...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2010.00842.x
更新日期：2011-01-01 00:00:00
abstract：:Time-resolved reflectance and transmittance spectroscopy was applied to measure in vivo the absorption and transport scattering spectra of the female breast from 610 to 1010 nm. Three measurement configurations were used to probe different breast regions, and data were collected two or three times in each of the five ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12166
更新日期：2014-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1751-1097.1990.tb01688.x
更新日期：1990-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2012.01197.x
更新日期：2013-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2010.00817.x
更新日期：2010-11-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)071<0237:aotbfp>2.0.co;2
更新日期：2000-02-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.12080
更新日期：2013-07-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12430
更新日期：2015-07-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1989.tb04162.x
更新日期：1989-09-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2009.00580.x
更新日期：2009-09-01 00:00:00
abstract：:A novel In(3+)-doped TiO(2) and TiO(2)/In(2)S(3) nanocomposites for photocatalytic degradation of environmental pollutants and stoichiometric degradation of warfare agents were prepared by a homogeneous hydrolysis with urea and thioacetamide, respectively. The prepared samples series TiInTAA were annealed at 600°C. Th...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.01052.x
更新日期：2012-03-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2012.01231.x
更新日期：2013-03-01 00:00:00
abstract：:Depletion of the ozone layer leads to increasing UV-B radiation on the earth's surface, which may affect weeds and their responses to herbicides. However, the effect of increased UV-B radiation on weeds and the interaction of weeds and herbicides are still obscure. The objective of this study was to compare glyphosate...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2012.01234.x
更新日期：2012-11-01 00:00:00
abstract：:The significance of human cutaneous pigmentation lies in its protective role against sun-induced DNA damage and photocarcinogenesis. Fair skin and red hair are characterized by a low eumelanin to pheomelanin ratio, and have been associated with increased risk of skin cancer. Cutaneous pigmentation is a complex genetic...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2011.00970.x
更新日期：2011-09-01 00:00:00
abstract：:Excessive exposure of solar ultraviolet (UV) radiation, particularly its UV-B component, to humans causes many adverse effects that include erythema, hyperplasia, hyperpigmentation, immunosuppression, photoaging and skin cancer. In recent years, there is increasing use of botanical agents in skin care products. Pomegr...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-08-06-RA-264
更新日期：2005-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2005-08-08-RA-641
更新日期：2006-05-01 00:00:00
abstract：:Channelrhodopsins act as photoreceptors for control of motility behavior in flagellates and are widely used as genetically targeted tools to optically manipulate the membrane potential of specific cell populations ("optogenetics"). The first two channelrhodopsins were obtained from the model organism Chlamydomonas rei...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.01027.x
更新日期：2012-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2005-05-10-RA-522
更新日期：2005-09-01 00:00:00
abstract：:Unlike the enchanting yellow-green flashes of light produced on warm summer evenings by Photinus pyralis, the most common firefly species in North America, the orange lights of Photinus scintillans are infrequently observed. These Photinus species, and likely all bioluminescent beetles, use the same substrates beetle ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12671
更新日期：2017-03-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2008.00315.x
更新日期：2008-07-01 00:00:00
abstract：:The incidence and prevalence of inflammatory bowel disease (IBD) are increasing worldwide. Some ecological studies show increasing incidence with increasing latitude. Ambient ultraviolet radiation varies inversely with latitude, and sun exposure of the skin is a major source of vitamin D. Vitamin D deficiency is commo...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.13007
更新日期：2019-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2001)074<0712:iosnri>2.0.co;2
更新日期：2001-11-01 00:00:00
abstract：:By a technique which combines rapid mixing of cells and hematoporphyrin (HP) with a short duration of illumination, the photodynamic inactivation of yeast cells was investigated, particularly, in seeking for the information of the location of HP at the time of action. The fluence-survival curves obtained under the con...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1981-10-01 00:00:00
abstract：:Changes in the emission fluorescence intensity of pheophorbide-a (PHEO) in the presence of carboquone (CARBOQ) were used to obtain the association constant, the number of CARBOQ molecules interacting with PHEO, and the fluorescence quantum yield of the complex. Excitation spectra of mixtures of PHEO and CARBOQ in etha...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2010.00869.x
更新日期：2011-03-01 00:00:00
abstract：:Photodynamic therapy (PDT), carried out at low fluence rates, may enhance tumor response as well as affect treatment selectivity. We have studied the effects of fluence rate on the response of the murine radiation-induced fibrosarcoma (RIF) to PDT using Photofrin (5 mg/kg). Tumor response was tested over a large range...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：:Solid-state NMR spectroscopy gives a powerful avenue for investigating G protein-coupled receptors and other integral membrane proteins in a native-like environment. This article reviews the use of solid-state (2)H NMR to study the retinal cofactor of rhodopsin in the dark state as well as the meta I and meta II photo...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2008.00510.x
更新日期：2009-03-01 00:00:00
